NM_001167910.1;0610038M03Rik; AV003995; mKIAA0091; S1P;SKI-1; Ski1
100μg liquid plasmid(Instantly):$120
| name | pCMV-MBTPS1(mouse)-3×FLAG-Neo | another name | apCMV-MBTPS1(mouse)-3×FLAG-Neo |
| Insert Size (bp) | 3159bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC ACCATGAAGC TCGTCAGCAC CTGGCTTCTT GTGCTGGTGG TTTTGCTCTG TGGGAAACGG CACCTGGGCG ACAGGCTGGG GACGAGAGCT TTGGAAAAGG CCCCGTGCCC CAGCTGCTCC CACCTGACTT TGAAGGTGGA ATTCTCTTCA ACTGTGGTGG AGTACGAATA TATTGTGGCT TTCAACGGAT ACTTCACAGC CAAAGCTAGA AACTCATTTA TTTCAAGTGC TCTGAAAAGC AGTGAAGTGG AAAACTGGAG AATAATACCT CGGAACAACC CATCCAGTGA CTACCCTAGT GATTTTGAGG TGATTCAGAT AAAAGAGAAG CAGAAGGCGG GGCTGCTCAC ACTTGAAGAT CACCCCAACA TCAAGCGGGT GACACCCCAG CGGAAAGTCT TTCGTTCCCT CAAGTTTGCT GAATCCAACC CCATCGTGCC CTGTAATGAA ACCCGGTGGA GCCAGAAGTG GCAGTCATCA CGTCCCCTGA AAAGAGCCAG TCTCTCCCTG GGCTCTGGAT TCTGGCATGC AACAGGAAGA CATTCAAGTC GGCGATTGCT GAGAGCCATT CCTCGCCAGG TCGCCCAGAC ACTGCAGGCA GATGTGCTGT GGCAGATGGG ATACACAGGT GCTAATGTCA GAGTTGCTGT TTTTGATACT GGGCTCAGTG AGAAGCATCC GCATTTTAAG AATGTGAAGG AGAGAACCAA CTGGACCAAT GAGCGGACCC TGGATGATGG GCTAGGCCAT GGCACATTCG TTGCAGGTGT GATTGCCAGC ATGAGGGAGT GCCAAGGATT TGCTCCAGAT GCAGAGCTGC ACATCTTCAG GGTCTTTACC AACAATCAGG TGTCTTACAC ATCTTGGTTT CTGGATGCCT TCAACTATGC CATCCTAAAG AAGATGGACG TTCTCAACCT TAGCATCGGT GGGCCCGACT TCATGGATCA TCCGTTTGTT GACAAGGTGT GGGAATTAAC AGCTAACAAT GTAATTATGG TTTCTGCTAT TGGCAATGAT GGACCTCTCT ATGGCACTCT GAATAACCCT GCTGATCAGA TGGATGTGAT TGGAGTGGGT GGCATTGACT TTGAAGATAA CATCGCTCGC TTTTCTTCCA GGGGAATGAC TACCTGGGAA TTACCAGGAG GCTATGGTCG TGTGAAGCCT GACATTGTCA CCTATGGTGC TGGAGTGCGG GGTTCCGGTG TGAAAGGGGG CTGCCGTGCA CTCTCAGGGA CCAGTGTCGC TTCCCCAGTG GTCGCTGGGG CCGTCACCTT GTTAGTAAGC ACAGTACAGA AGCGGGAGCT GGTGAATCCT GCCAGTGTGA AGCAAGCTTT GATAGCGTCA GCCCGGAGAC TTCCTGGGGT CAACATGTTC GAGCAAGGTC ATGGCAAGTT GGATCTGCTG CGAGCTTATC AGATCCTCAG CAGCTATAAA CCGCAGGCAA GCCTGAGTCC TAGCTACATC GACCTGACTG AGTGTCCCTA CATGTGGCCC TACTGCTCCC AGCCTATCTA CTATGGAGGA ATGCCAACAA TCGTTAATGT CACCATCCTC AATGGCATGG GCGTCACAGG AAGAATTGTG GATAAGCCTG AGTGGCGACC CTATTTACCA CAGAATGGAG ACAACATTGA AGTGGCCTTC TCCTACTCCT CAGTGTTGTG GCCCTGGTCA GGTTACCTTG CCATCTCCAT TTCTGTGACC AAGAAGGCAG CTTCCTGGGA AGGCATCGCT CAGGGCCACA TCATGATCAC AGTGGCGTCC CCAGCAGAGA CAGAGTTACA CAGTGGTGCG GAGCACACTT CCACCGTGAA GCTGCCCATC AAGGTGAAGA TCATTCCCAC CCCTCCTCGG AGCAAGAGAG TCCTCTGGGA CCAGTACCAC AACCTCCGCT ACCCACCTGG CTACTTCCCC AGGGACAACT TGCGGATGAA GAATGACCCT TTAGACTGGA ATGGCGACCA CGTCCACACC AACTTCAGGG ACATGTACCA GCATCTGCGC AGCATGGGCT ACTTCGTGGA GGTGCTCGGC GCCCCATTCA CATGTTTTGA CGCCACACAG TATGGCACTT TGCTGCTGGT GGACAGTGAG GAAGAGTACT TCCCTGAGGA GATTGCTAAG CTGAGGAGGG ATGTGGACAA TGGCCTTTCC CTCGTCATCT TCAGTGACTG GTACAACACT TCTGTTATGA GAAAAGTGAA GTTTTATGAT GAAAACACCA GGCAGTGGTG GATGCCAGAC ACCGGAGGAG CGAACATCCC AGCTCTGAAT GAGCTGCTGT CTGTGTGGAA CATGGGGTTC AGTGACGGCC TATATGAAGG GGAGTTTGTC CTGGCAAACC ATGACATGTA CTATGCGTCG GGGTGCAGCA TCGCCAAGTT TCCAGAAGAT GGCGTCGTGA TCACACAGAC TTTCAAGGAC CAAGGATTGG AGGTCTTAAA ACAAGAGACA GCAGTTGTGG AAAATGTTCC CATTTTGGGG CTTTATCAGA TTCCATCTGA AGGTGGAGGC CGGATCGTGC TGTATGGAGA CTCCAACTGC TTGGATGACA GTCACAGACA GAAGGACTGC TTTTGGCTTC TGGATGCGCT CCTTCAGTAC ACATCCTATG GCGTGACCCC TCCCAGCCTC AGCCATTCAG GGAACCGGCA GCGCCCACCT AGCGGAGCCG GCTTGGCCCC TCCTGAAAGG ATGGAAGGAA ACCACCTCCA TCGGTACTCC AAAGTTCTTG AAGCCCACTT GGGAGACCCG AAACCTCGGC CCCTGCCAGC CTGTCCACAT TTGTCATGGG CCAAGCCACA GCCTTTGAAT GAGACGGCAC CCAGTAATCT TTGGAAACAT CAGAAGCTGC TCTCCATTGA CCTGGACAAA GTAGTGTTAC CCAACTTTCG ATCCAATCGC CCTCAAGTGA GACCTTTGTC CCCTGGAGAG AGTGGTGCCT GGGACATTCC TGGAGGGATC ATGCCTGGCC GCTACAACCA GGAGGTGGGA CAGACCATCC CCGTCTTCGC CTTCCTCGGA GCCATGGTGG CCCTGGCCTT CTTTGTGGTA CAGATCAGCA AGGCCAAGAG CCGGCCGAAG CGGAGGAGGC CCAGGGCAAA GCGTCCACAA CTTGCACAGC AGGCCCACCC TGCAAGGACC CCATCAGTGC TCGAGGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
