pAAV-RUNX2(mouse)-Promoter-EGFP
$84.00
In stock
SKU
PL49190
100μg liquid plasmid(Instantly):$105
| name | pAAV-RUNX2(mouse)-Promoter-EGFP | another name | apAAV-RUNX2(mouse)-Promoter-EGFP |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | RUNX2 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGGAGC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG ACAATACATA AAGGCCACGT TCAGCAACCT CTACTAAACT CTTGTATCAT GAGATACAGA CCATAACTCA CAGACACAGG AAATAGGGTT AGGTTACCTG CAAAACAGAC CATGTGACTA CTCATCTGAT AAATGAGATG GTGGTAAAAT TTATTCAAAT TCATTTTAGA TCAATTTGAA TACCAAAATG TATATACCTT TTTTTTAAAA AATGTAAGGG AAAAATTATT GCATGAAAAA TTAGGACATA AAAGACCTGG GCACTCTAAA AGAAAAGCAT TTGCTTACTA TCCTATAGCA ACTACGCAAA CATCTTCAAC TGCCAAGTGC TGCGATTCCT TGTACATATG GAACTAAGTT CAGAAACTCC ACAAATTATA TAGACAAAAC CCTTTTTTTA TTTACTTTGA ATAATAGAGA TAAAGATCAC ACTGGCACAC TTTATTTATG AAAGAGGATA ATAGAGTAAC TTTTTTCTCC TCTGCATGAA TAATGACCCT AAATGAAAAC TTCAGTATAA ATATCTGTTT TACAGTAAAA CATGAGTCTA GCCTCAAAAA TCAAACAAAA GAATGTATTT CTGTGGTTTT GTCATTAAAA CTTTATTCTG AAAAATTAAA TAAATAAACC TAGATTCTTG AAAAATAAGG GGTTAAAAGC ATTACCATGT CTTTCCAGTA TATAGAGAAT AAATGTTTAA AGAATCTTAT GAACATGATT TCATAGATAA CTTTAACTAA GAGGAAACAA AAACAGACAA TGAGTTATTT TGGGGTGTAC AGACACAAGA ATATTTTACT TCTGTCACCC TCTAAGTCAC TCCCTCTTAC CTCCACTGTG CACCCCAAAT AATTTCTTGT ACTTCTGTGC CCCCACCCAC CATCACAGTC ATCCGTTCCA TGCCACTCCT GGTTACCATC ACACTAGGAA GAAATCTAAC ATGCAAATTC AGAGTGGCGT GGATAAATGG CAAAAAATGC CTAGGAAATT GGTCTGCTCG CCTTTATAAT GTTTGTTGAA AAATCCTCCA TCGCTCCCAA CTAATGAAAA CAGGAAGCTC TATTCATAAA TGTGAAATTC ACTGCCTATG ATATATAATC ATCCTAATAA GAAAATGAGC TCTAGACATA CATGTCCAAG AGGGCAAAAG AAGAGATAGT TTCCCAAAGA TGGTTTCAAT TTTCTTCTGA ATCAGAATTA GCAAATCAAG ACGACTAACA TACTCTGTCT GTGTGCATTA TTCCTTACTA CACACAGCAT TTTGTAATTT ATTTCAAAGC TTCCATTATA AACAACAAAA ACTTACAGTT TCTGTTAACC CCCTCTATTC TGAGCTATGG AAATTACTGC ATATTTCATT ATATATGCAG AACTGCACCC AAAGTCCTGT TACAGTCACT GTCCACGCTG ATGAAAGAAT TATACAAAAC ATTTCTTTGA AAGATAAAAT CCAATCATAC AGAAAACTAA CATTAGTCCA ACAAAATGTC CACCACAATT CCTGACATTT GTTTTTTAAG ATCTTCAAAG TAACCATGGG ATGATGGCAA AAATAATGTA AACGATACTA ATTACATTTA ATCTTTATTG TAAGAGCCGC CACGTAATAA AAAAAAAAAA ATCAACTACA CAGCCATGAT TTAATATTTG TAAAGGAATC CCCAGGCTAA CACTTTTGTG ACAGCCAATT ACAGTCGATC CCGATCCCGG CAAGGAGTTT GCAAGCAGAG CTCTGGAAAG GTAAACTCCT TTTTACAATG AGTTACAGAT CCCCAAGCTT AGGAAGACAA GCAAAAGGCA AACAGAAGGA AGCAGCCACC CTGGGAAATC CGAAGCAGCC TTGCAAGTGA TACAATCCCA AGATGCGAAT TACTGCAAAG CAGCACTGTT GCTCAGAACG CCACACACTC AGTTGAGACA ATTTTGCTCA CTTTTCCATA GACATAATAA TGAAGGAAAG GGAGGAGGGG TAGAGAAGAG AGATGAAAAA GCAGAGGAGG GAAGGGGGAG TAGGGAGGTG GCAGAAAGGA AAAGCCTTAG CTACAGAGTT CTGCTCTCCA GAGGCTTAAC CTTACAGGAG TGTGGGCTCC TTCAGCATTT GTGTTCTAGC CAAATCCTCA TGAGTCACAA AAATTAAAAA GCTATAACCT TCTGAATGCC AGGAAGGCCT TACCACAAGC CTTTTGTCAG AGGGAGAAAG GGAGAGAGAG AGGGAGAGAG AGAGGGAGAG AGAGAGGGAG AGAGGAAGGG AAGGAGAGAC AGAGGAACAC CCATAAGTAA AGAGACAGAA GGAAGGAAAG GGAGAGGACA AGAGAAGAGA AAGGAGGGAG GGGAGGGGAG AAGGAAAAAG ATTGAGAAAG AGGGAGGGAA GAGAGCAAGG GGGAAGCCAC AGTGGTAGGC AGTCCCACTT TACTTTGAGT ACTGTGAGGT CACAAACCAC ATGATTCTGT CTCTCCAGTA ATAGTGCTTG CAAAAAATAG GAGTTTTAAA GCTTTTGCTT TTTTGGATTG TGTGTTCGAA CATCGATTGA ATTCTGAATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTACTCA GATCTCGAGC TCAAGTAGGG ATCCTCTAGA GTCGACCTGC AGAAGCTTGC CTCGAGCAGC GCTGCTCGAG AGATCTACGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTGTAGGTA ACCACGTGCG GACCGAGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GT
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