pLV3-CMV-IGF1R(human)-CopGFP-Puro
$113.00
In stock
SKU
PL49174
NM_000875.5;CD221; IGFIR; IGFR; JTK13
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-IGF1R(human)-CopGFP-Puro | another name | apLV3-CMV-IGF1R(human)-CopGFP-Puro |
| Insert Size (bp) | 4104bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGA AGTCTGGCTC CGGAGGAGGG TCCCCGACCT CGCTGTGGGG GCTCCTGTTT CTCTCCGCCG CGCTCTCGCT CTGGCCGACG AGTGGAGAAA TCTGCGGGCC AGGCATCGAC ATCCGCAACG ACTATCAGCA GCTGAAGCGC CTGGAGAACT GCACGGTGAT CGAGGGCTAC CTCCACATCC TGCTCATCTC CAAGGCCGAG GACTACCGCA GCTACCGCTT CCCCAAGCTC ACGGTCATTA CCGAGTACTT GCTGCTGTTC CGAGTGGCTG GCCTCGAGAG CCTCGGAGAC CTCTTCCCCA ACCTCACGGT CATCCGCGGC TGGAAACTCT TCTACAACTA CGCCCTGGTC ATCTTCGAGA TGACCAATCT CAAGGATATT GGGCTTTACA ACCTGAGGAA CATTACTCGG GGGGCCATCA GGATTGAGAA AAATGCTGAC CTCTGTTACC TCTCCACTGT GGACTGGTCC CTGATCCTGG ATGCGGTGTC CAATAACTAC ATTGTGGGGA ATAAGCCCCC AAAGGAATGT GGGGACCTGT GTCCAGGGAC CATGGAGGAG AAGCCGATGT GTGAGAAGAC CACCATCAAC AATGAGTACA ACTACCGCTG CTGGACCACA AACCGCTGCC AGAAAATGTG CCCAAGCACG TGTGGGAAGC GGGCGTGCAC CGAGAACAAT GAGTGCTGCC ACCCCGAGTG CCTGGGCAGC TGCAGCGCGC CTGACAACGA CACGGCCTGT GTAGCTTGCC GCCACTACTA CTATGCCGGT GTCTGTGTGC CTGCCTGCCC GCCCAACACC TACAGGTTTG AGGGCTGGCG CTGTGTGGAC CGTGACTTCT GCGCCAACAT CCTCAGCGCC GAGAGCAGCG ACTCCGAGGG GTTTGTGATC CACGACGGCG AGTGCATGCA GGAGTGCCCC TCGGGCTTCA TCCGCAACGG CAGCCAGAGC ATGTACTGCA TCCCTTGTGA AGGTCCTTGC CCGAAGGTCT GTGAGGAAGA AAAGAAAACA AAGACCATTG ATTCTGTTAC TTCTGCTCAG ATGCTCCAAG GATGCACCAT CTTCAAGGGC AATTTGCTCA TTAACATCCG ACGGGGGAAT AACATTGCTT CAGAGCTGGA GAACTTCATG GGGCTCATCG AGGTGGTGAC GGGCTACGTG AAGATCCGCC ATTCTCATGC CTTGGTCTCC TTGTCCTTCC TAAAAAACCT TCGCCTCATC CTAGGAGAGG AGCAGCTAGA AGGGAATTAC TCCTTCTACG TCCTCGACAA CCAGAACTTG CAGCAACTGT GGGACTGGGA CCACCGCAAC CTGACCATCA AAGCAGGGAA AATGTACTTT GCTTTCAATC CCAAATTATG TGTTTCCGAA ATTTACCGCA TGGAGGAAGT GACGGGGACT AAAGGGCGCC AAAGCAAAGG GGACATAAAC ACCAGGAACA ACGGGGAGAG AGCCTCCTGT GAAAGTGACG TCCTGCATTT CACCTCCACC ACCACGTCGA AGAATCGCAT CATCATAACC TGGCACCGGT ACCGGCCCCC TGACTACAGG GATCTCATCA GCTTCACCGT TTACTACAAG GAAGCACCCT TTAAGAATGT CACAGAGTAT GATGGGCAGG ATGCCTGCGG CTCCAACAGC TGGAACATGG TGGACGTGGA CCTCCCGCCC AACAAGGACG TGGAGCCCGG CATCTTACTA CATGGGCTGA AGCCCTGGAC TCAGTACGCC GTTTACGTCA AGGCTGTGAC CCTCACCATG GTGGAGAACG ACCATATCCG TGGGGCCAAG AGTGAGATCT TGTACATTCG CACCAATGCT TCAGTTCCTT CCATTCCCTT GGACGTTCTT TCAGCATCGA ACTCCTCTTC TCAGTTAATC GTGAAGTGGA ACCCTCCCTC TCTGCCCAAC GGCAACCTGA GTTACTACAT TGTGCGCTGG CAGCGGCAGC CTCAGGACGG CTACCTTTAC CGGCACAATT ACTGCTCCAA AGACAAAATC CCCATCAGGA AGTATGCCGA CGGCACCATC GACATTGAGG AGGTCACAGA GAACCCCAAG ACTGAGGTGT GTGGTGGGGA GAAAGGGCCT TGCTGCGCCT GCCCCAAAAC TGAAGCCGAG AAGCAGGCCG AGAAGGAGGA GGCTGAATAC CGCAAAGTCT TTGAGAATTT CCTGCACAAC TCCATCTTCG TGCCCAGACC TGAAAGGAAG CGGAGAGATG TCATGCAAGT GGCCAACACC ACCATGTCCA GCCGAAGCAG GAACACCACG GCCGCAGACA CCTACAACAT CACCGACCCG GAAGAGCTGG AGACAGAGTA CCCTTTCTTT GAGAGCAGAG TGGATAACAA GGAGAGAACT GTCATTTCTA ACCTTCGGCC TTTCACATTG TACCGCATCG ATATCCACAG CTGCAACCAC GAGGCTGAGA AGCTGGGCTG CAGCGCCTCC AACTTCGTCT TTGCAAGGAC TATGCCCGCA GAAGGAGCAG ATGACATTCC TGGGCCAGTG ACCTGGGAGC CAAGGCCTGA AAACTCCATC TTTTTAAAGT GGCCGGAACC TGAGAATCCC AATGGATTGA TTCTAATGTA TGAAATAAAA TACGGATCAC AAGTTGAGGA TCAGCGAGAA TGTGTGTCCA GACAGGAATA CAGGAAGTAT GGAGGGGCCA AGCTAAACCG GCTAAACCCG GGGAACTACA CAGCCCGGAT TCAGGCCACA TCTCTCTCTG GGAATGGGTC GTGGACAGAT CCTGTGTTCT TCTATGTCCA GGCCAAAACA GGATATGAAA ACTTCATCCA TCTGATCATC GCTCTGCCCG TCGCTGTCCT GTTGATCGTG GGAGGGTTGG TGATTATGCT GTACGTCTTC CATAGAAAGA GAAATAACAG CAGGCTGGGG AATGGAGTGC TGTATGCCTC TGTGAACCCG GAGTACTTCA GCGCTGCTGA TGTGTACGTT CCTGATGAGT GGGAGGTGGC TCGGGAGAAG ATCACCATGA GCCGGGAACT TGGGCAGGGG TCGTTTGGGA TGGTCTATGA AGGAGTTGCC AAGGGTGTGG TGAAAGATGA ACCTGAAACC AGAGTGGCCA TTAAAACAGT GAACGAGGCC GCAAGCATGC GTGAGAGGAT TGAGTTTCTC AACGAAGCTT CTGTGATGAA GGAGTTCAAT TGTCACCATG TGGTGCGATT GCTGGGTGTG GTGTCCCAAG GCCAGCCAAC ACTGGTCATC ATGGAACTGA TGACACGGGG CGATCTCAAA AGTTATCTCC GGTCTCTGAG GCCAGAAATG GAGAATAATC CAGTCCTAGC ACCTCCAAGC CTGAGCAAGA TGATTCAGAT GGCCGGAGAG ATTGCAGACG GCATGGCATA CCTCAACGCC AATAAGTTCG TCCACAGAGA CCTTGCTGCC CGGAATTGCA TGGTAGCCGA AGATTTCACA GTCAAAATCG GAGATTTTGG TATGACGCGA GATATCTATG AGACAGACTA TTACCGGAAA GGAGGGAAAG GGCTGCTGCC CGTGCGCTGG ATGTCTCCTG AGTCCCTCAA GGATGGAGTC TTCACCACTT ACTCGGACGT CTGGTCCTTC GGGGTCGTCC TCTGGGAGAT CGCCACACTG GCCGAGCAGC CCTACCAGGG CTTGTCCAAC GAGCAAGTCC TTCGCTTCGT CATGGAGGGC GGCCTTCTGG ACAAGCCAGA CAACTGTCCT GACATGCTGT TTGAACTGAT GCGCATGTGC TGGCAGTATA ACCCCAAGAT GAGGCCTTCC TTCCTGGAGA TCATCAGCAG CATCAAAGAG GAGATGGAGC CTGGCTTCCG GGAGGTCTCC TTCTACTACA GCGAGGAGAA CAAGCTGCCC GAGCCGGAGG AGCTGGACCT GGAGCCAGAG AACATGGAGA GCGTCCCCCT GGACCCCTCG GCCTCCTCGT CCTCCCTGCC ACTGCCCGAC AGACACTCAG GACACAAGGC CGAGAACGGC CCCGGCCCTG GGGTGCTGGT CCTCCGCGCC AGCTTCGACG AGAGACAGCC TTACGCCCAC ATGAACGGGG GCCGCAAGAA CGAGCGGGCC TTGCCGCTGC CCCAGTCTTC GACCTGCTGA GGATCCCTGC GATATCCAGC GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ACGCCACCAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCAAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGGTG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGTGCTGG TGGGCAGCTT CGCCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TTCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGCTGC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCCATCGCCT TCGCCAGATC CCGCGCTCAG TCGTCCAATT CTGCCGTGGA CGGCACCGCC GGACCCGGCT CCACCGGATC TCGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTCCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
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