pmini35S-MCS-GUS-6×His-Hyg
$84.00
In stock
SKU
PL48962
100μg liquid plasmid(Instantly):$105
| name | pmini35S-MCS-GUS-6×His-Hyg | another name | apmini35S-MCS-GUS-6×His-Hyg |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Transcription factor reporting | Fragment type | Element |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | Gus |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCAAAAGGA CAGTAGAAAA GGAAGGTGGC ACCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGGTAATA TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCAA AAGGACAGTA GAAAAGGAAG GTGGCACCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCGTTCAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGATAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTGAAAT CACCAGTCTC TCTCTACAAA TCTATCTCTC TCGAGCTTTC GCAGATCCCG GGGGGCAATG AGATATGAAA AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAG TTTAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTA CAACCGGTCG CGGAGGCTAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCACGACTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGAAATAG AGTAGATGCC GACCGGATCT GTCGATCGAC AAGCTCGAGT TTCTCCATAA TAATGTGTGA GTAGTTCCCA GATAAGGGAA TTAGGGTTCC TATAGGGTTT CGCTCATGTG TTGAGCATAT AAGAAACCCT TAGTATGTAT TTGTATTTGT AAAATACTTC TATCAATAAA ATTTCTAATT CCTAAAACCA AAATCCAGTA CTAAAATCCA GATCCCCCGA ATTAATTCGG CGTTAATTCA GTACATTAAA AACGTCCGCA ATGTGTTATT AAGTTGTCTA AGCGTCAATT TGTTTACACC ACAATATATC CTGCCACCAG CCAGCCAACA GCTCCCCGAC CGGCAGCTCG GCACAAAATC ACCACTCGAT ACAGGCAGCC CATCAGTCCG GGACGGCGTC AGCGGGAGAG CCGTTGTAAG GCGGCAGACT TTGCTCATGT TACCGATGCT ATTCGGAAGA ACGGCAACTA AGCTGCCGGG TTTGAAACAC GGATGATCTC GCGGAGGGTA GCATGTTGAT TGTAACGATG ACAGAGCGTT GCTGCCTGTG ATCACCGCGG TTTCAAAATC GGCTCCGTCG ATACTATGTT ATACGCCAAC TTTGAAAACA ACTTTGAAAA AGCTGTTTTC TGGTATTTAA GGTTTTAGAA TGCAAGGAAC AGTGAATTGG AGTTCGTCTT GTTATAATTA GCTTCTTGGG GTATCTTTAA ATACTGTAGA AAAGAGGAAG GAAATAATAA ATGGCTAAAA TGAGAATATC ACCGGAATTG AAAAAACTGA TCGAAAAATA CCGCTGCGTA AAAGATACGG AAGGAATGTC TCCTGCTAAG GTATATAAGC TGGTGGGAGA AAATGAAAAC CTATATTTAA AAATGACGGA CAGCCGGTAT AAAGGGACCA CCTATGATGT GGAACGGGAA AAGGACATGA TGCTATGGCT GGAAGGAAAG CTGCCTGTTC CAAAGGTCCT GCACTTTGAA CGGCATGATG GCTGGAGCAA TCTGCTCATG AGTGAGGCCG ATGGCGTCCT TTGCTCGGAA GAGTATGAAG ATGAACAAAG CCCTGAAAAG ATTATCGAGC TGTATGCGGA GTGCATCAGG CTCTTTCACT CCATCGACAT ATCGGATTGT CCCTATACGA ATAGCTTAGA CAGCCGCTTA GCCGAATTGG ATTACTTACT GAATAACGAT CTGGCCGATG TGGATTGCGA AAACTGGGAA GAAGACACTC CATTTAAAGA TCCGCGCGAG CTGTATGATT TTTTAAAGAC GGAAAAGCCC GAAGAGGAAC TTGTCTTTTC CCACGGCGAC CTGGGAGACA GCAACATCTT TGTGAAAGAT GGCAAAGTAA GTGGCTTTAT TGATCTTGGG AGAAGCGGCA GGGCGGACAA GTGGTATGAC ATTGCCTTCT GCGTCCGGTC GATCAGGGAG GATATCGGGG AAGAACAGTA TGTCGAGCTA TTTTTTGACT TACTGGGGAT CAAGCCTGAT TGGGAGAAAA TAAAATATTA TATTTTACTG GATGAATTGT TTTAGTACCT AGAATGCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGGGT GCCTTGATGT GGGCGCCGGC GGTCGAGTGG CGACGGCGCG GCTTGTCCGC GCCCTGGTAG ATTGCCTGGC CGTAGGCCAG CCATTTTTGA GCGGCCAGCG GCCGCGATAG GCCGACGCGA AGCGGCGGGG CGTAGGGAGC GCAGCGACCG AAGGGTAGGC GCTTTTTGCA GCTCTTCGGC TGTGCGCTGG CCAGACAGTT ATGCACAGGC CAGGCGGGTT TTAAGAGTTT TAATAAGTTT TAAAGAGTTT TAGGCGGAAA AATCGCCTTT TTTCTCTTTT ATATCAGTCA CTTACATGTG TGACCGGTTC CCAATGTACG GCTTTGGGTT CCCAATGTAC GGGTTCCGGT TCCCAATGTA CGGCTTTGGG TTCCCAATGT ACGTGCTATC CACAGGAAAG AGACCTTTTC GACCTTTTTC CCCTGCTAGG GCAATTTGCC CTAGCATCTG CTCCGTACAT TAGGAACCGG CGGATGCTTC GCCCTCGATC AGGTTGCGGT AGCGCATGAC TAGGATCGGG CCAGCCTGCC CCGCCTCCTC CTTCAAATCG TACTCCGGCA GGTCATTTGA CCCGATCAGC TTGCGCACGG TGAAACAGAA CTTCTTGAAC TCTCCGGCGC TGCCACTGCG TTCGTAGATC GTCTTGAACA ACCATCTGGC TTCTGCCTTG CCTGCGGCGC GGCGTGCCAG GCGGTAGAGA AAACGGCCGA TGCCGGGATC GATCAAAAAG TAATCGGGGT GAACCGTCAG CACGTCCGGG TTCTTGCCTT CTGTGATCTC GCGGTACATC CAATCAGCTA GCTCGATCTC GATGTACTCC GGCCGCCCGG TTTCGCTCTT TACGATCTTG TAGCGGCTAA TCAAGGCTTC ACCCTCGGAT ACCGTCACCA GGCGGCCGTT CTTGGCCTTC TTCGTACGCT GCATGGCAAC GTGCGTGGTG TTTAACCGAA TGCAGGTTTC TACCAGGTCG TCTTTCTGCT TTCCGCCATC GGCTCGCCGG CAGAACTTGA GTACGTCCGC AACGTGTGGA CGGAACACGC GGCCGGGCTT GTCTCCCTTC CCTTCCCGGT ATCGGTTCAT GGATTCGGTT AGATGGGAAA CCGCCATCAG TACCAGGTCG TAATCCCACA CACTGGCCAT GCCGGCCGGC CCTGCGGAAA CCTCTACGTG CCCGTCTGGA AGCTCGTAGC GGATCACCTC GCCAGCTCGT CGGTCACGCT TCGACAGACG GAAAACGGCC ACGTCCATGA TGCTGCGACT ATCGCGGGTG CCCACGTCAT AGAGCATCGG AACGAAAAAA TCTGGTTGCT CGTCGCCCTT GGGCGGCTTC CTAATCGACG GCGCACCGGC TGCCGGCGGT TGCCGGGATT CTTTGCGGAT TCGATCAGCG GCCGCTTGCC ACGATTCACC GGGGCGTGCT TCTGCCTCGA TGCGTTGCCG CTGGGCGGCC TGCGCGGCCT TCAACTTCTC CACCAGGTCA TCACCCAGCG CCGCGCCGAT TTGTACCGGG CCGGATGGTT TGCGACCGCT CACGCCGATT CCTCGGGCTT GGGGGTTCCA GTGCCATTGC AGGGCCGGCA GACAACCCAG CCGCTTACGC CTGGCCAACC GCCCGTTCCT CCACACATGG GGCATTCCAC GGCGTCGGTG CCTGGTTGTT CTTGATTTTC CATGCCGCCT CCTTTAGCCG CTAAAATTCA TCTACTCATT TATTCATTTG CTCATTTACT CTGGTAGCTG CGCGATGTAT TCAGATAGCA GCTCGGTAAT GGTCTTGCCT TGGCGTACCG CGTACATCTT CAGCTTGGTG TGATCCTCCG CCGGCAACTG AAAGTTGACC CGCTTCATGG CTGGCGTGTC TGCCAGGCTG GCCAACGTTG CAGCCTTGCT GCTGCGTGCG CTCGGACGGC CGGCACTTAG CGTGTTTGTG CTTTTGCTCA TTTTCTCTTT ACCTCATTAA CTCAAATGAG TTTTGATTTA ATTTCAGCGG CCAGCGCCTG GACCTCGCGG GCAGCGTCGC CCTCGGGTTC TGATTCAAGA ACGGTTGTGC CGGCGGCGGC AGTGCCTGGG TAGCTCACGC GCTGCGTGAT ACGGGACTCA AGAATGGGCA GCTCGTACCC GGCCAGCGCC TCGGCAACCT CACCGCCGAT GCGCGTGCCT TTGATCGCCC GCGACACGAC AAAGGCCGCT TGTAGCCTTC CATCCGTGAC CTCAATGCGC TGCTTAACCA GCTCCACCAG GTCGGCGGTG GCCCATATGT CGTAAGGGCT TGGCTGCACC GGAATCAGCA CGAAGTCGGC TGCCTTGATC GCGGACACAG CCAAGTCCGC CGCCTGGGGC GCTCCGTCGA TCACTACGAA GTCGCGCCGG CCGATGGCCT TCACGTCGCG GTCAATCGTC GGGCGGTCGA TGCCGACAAC GGTTAGCGGT TGATCTTCCC GCACGGCCGC CCAATCGCGG GCACTGCCCT GGGGATCGGA ATCGACTAAC AGAACATCGG CCCCGGCGAG TTGCAGGGCG CGGGCTAGAT GGGTTGCGAT GGTCGTCTTG CCTGACCCGC CTTTCTGGTT AAGTACAGCG ATAACCTTCA TGCGTTCCCC TTGCGTATTT GTTTATTTAC TCATCGCATC ATATACGCAG CGACCGCATG ACGCAAGCTG TTTTACTCAA ATACACATCA CCTTTTTAGA CGGCGGCGCT CGGTTTCTTC AGCGGCCAAG CTGGCCGGCC AGGCCGCCAG CTTGGCATCA GACAAACCGG CCAGGATTTC ATGCAGCCGC ACGGTTGAGA CGTGCGCGGG CGGCTCGAAC ACGTACCCGG CCGCGATCAT CTCCGCCTCG ATCTCTTCGG TAATGAAAAA CGGTTCGTCC TGGCCGTCCT GGTGCGGTTT CATGCTTGTT CCTCTTGGCG TTCATTCTCG GCGGCCGCCA GGGCGTCGGC CTCGGTCAAT GCGTCCTCAC GGAAGGCACC GCGCCGCCTG GCCTCGGTGG GCGTCACTTC CTCGCTGCGC TCAAGTGCGC GGTACAGGGT CGAGCGATGC ACGCCAAGCA GTGCAGCCGC CTCTTTCACG GTGCGGCCTT CCTGGTCGAT CAGCTCGCGG GCGTGCGCGA TCTGTGCCGG GGTGAGGGTA GGGCGGGGGC CAAACTTCAC GCCTCGGGCC TTGGCGGCCT CGCGCCCGCT CCGGGTGCGG TCGATGATTA GGGAACGCTC GAACTCGGCA ATGCCGGCGA ACACGGTCAA CACCATGCGG CCGGCCGGCG TGGTGGTGTC GGCCCACGGC TCTGCCAGGC TACGCAGGCC CGCGCCGGCC TCCTGGATGC GCTCGGCAAT GTCCAGTAGG TCGCGGGTGC TGCGGGCCAG GCGGTCTAGC CTGGTCACTG TCACAACGTC GCCAGGGCGT AGGTGGTCAA GCATCCTGGC CAGCTCCGGG CGGTCGCGCC TGGTGCCGGT GATCTTCTCG GAAAACAGCT TGGTGCAGCC GGCCGCGTGC AGTTCGGCCC GTTGGTTGGT CAAGTCCTGG TCGTCGGTGC TGACGCGGGC ATAGCCCAGC AGGCCAGCGG CGGCGCTCTT GTTCATGGCG TAATGTCTCC GGTTCTAGTC GCAAGTATTC TACTTTATGC GACTAAAACA CGCGACAAGA AAACGCCAGG AAAAGGGCAG GGCGGCAGCC TGTCGCGTAA CTTAGGACTT GTGCGACATG TCGTTTTCAG AAGACGGCTG CACTGAACGT CAGAAGCCGA CTGCACTATA GCAGCGGAGG GGTTGGATCA AAGTACTTTG ATCCCGAGGG GAACCCTGTG GTTGGCATGC ACATACAAAT GGACGAACGG ATAAACCTTT TCACGCCCTT TTAAATATCC GTTATTCTAA TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA ATTCCCGATC TAGTAACATA GATGACACCG CGCGCGATAA TTTATCCTAG TTTGCGCGCT ATATTTTGTT TTCTATCGCG TATTAAATGT ATAATTGCGG GACTCTAATC ATAAAAACCC ATCTCATAAA TAACGTCATG CATTACATGT TAATTATTAC ATGCTTAACG TAATTCAACA GAAATTATAT GATAATCATC GCAAGACCGG CAACAGGATT CAATCTTAAG AAACTTTATT GCCAAATGTT TGAACGATCG GGGAAATTCG AGCTGGTCAC CAATTCACAC GTGGTGGTGG TGGTGGTGGC TAGCTTGTTT GCCTCCCTGC TGCGGTTTTT CACCGAAGTT CATGCCAGTC CAGCGTTTTT GCAGCAGAAA AGCCGCCGAC TTCGGTTTGC GGTCGCGAGT GAAGATCCCT TTCTTGTTAC CGCCAACGCG CAATATGCCT TGCGAGGTCG CAAAATCGGC GAAATTCCAT ACCTGTTCAC CGACGACGGC GCTGACGCGA TCAAAGACGC GGTGATACAT ATCCAGCCAT GCACACTGAT ACTCTTCACT CCACATGTCG GTGTACATTG AGTGCAGCCC GGCTAACGTA TCCACGCCGT ATTCGGTGAT GATAATCGGC TGATGCAGTT TCTCCTGCCA GGCCAGAAGT TCTTTTTCCA GTACCTTCTC TGCCGTTTCC AAATCGCCGC TTTGGACATA CCATCCGTAA TAACGGTTCA GGCACAGCAC ATCAAAGAGA TCGCTGATGG TATCGGTGTG AGCGTCGCAG AACATTACAT TGACGCAGGT GATCGGACGC GTCGGGTCGA GTTTACGCGT TGCTTCCGCC AGTGGCGCGA AATATTCCCG TGCACCTTGC GGACGGGTAT CCGGTTCGTT GGCAATACTC CACATCACCA CGCTTGGGTG GTTTTTGTCA CGCGCTATCA GCTCTTTAAT CGCCTGTAAG TGCGCTTGCT GAGTTTCCCC GTTGACTGCC TCTTCGCTGT ACAGTTCTTT CGGCTTGTTG CCCGCTTCGA AACCAATGCC TAAAGAGAGG TTAAAGCCGA CAGCAGCAGT TTCATCAATC ACCACGATGC CATGTTCATC TGCCCAGTCG AGCATCTCTT CAGCGTAAGG GTAATGCGAG GTACGGTAGG AGTTGGCCCC AATCCAGTCC ATTAATGCGT GGTCGTGCAC CATCAGCACG TTATCGAATC CTTTGCCACG CAAGTCCGCA TCTTCATGAC GACCAAAGCC AGTAAAGTAG AACGGTTTGT GGTTAATCAG GAACTGTTCG CCCTTCACTG CCACTGACCG GATGCCGACG CGAAGCGGGT AGATATCACA CTCTGTCTGG CTTTTGGCTG TGACGCACAG TTCATAGAGA TAACCTTCAC CCGGTTGCCA GAGGTGCGGA TTCACCACTT GCAAAGTCCC GCTAGTGCCT TGTCCAGTTG CAACCACCTG TTGATCCGCA TCACGCAGTT CAACGCTGAC ATCACCATTG GCCACCACCT GCCAGTCAAC AGACGCGTGG TTACAGTCTT GCGCGACATG CGTCACCACG GTGATATCGT CCACCCAGGT GTTCGGCGTG GTGTAGAGCA TTACGCTGCG ATGGATTCCG GCATAGTTAA AGAAATCATG GAAGTAAGAC TGCTTTTTCT TGCCGTTTTC GTCGGTAATC ACCATTCCCG GCGGGATAGT CTGCCAGTTC AGTTCGTTGT TCACACAAAC GGTGATACGT ACACTTTTCC CGGCAATAAC ATACGGCGTG ACATCGGCTT CAAATGGCGT ATAGCCGCCC TGATGCTCCA TCACTTCCTG ATTATTGACC CACACTTTGC CGTAATGAGT GACCGCATCG AAACGCAGCA CGATACGCTG GCCTGCCCAA CCTTTCGGTA TAAAGACTTC GCGCTGATAC CAGACGTTGC CCGCATAATT ACGAATATCT GCATCGGCGA ACTGATCGTT AAAACTGCCT GGCACAGCAA TTGCCCGGCT TTCTTGTAAC GCGCTTTCCC ACCAACGCTG ATCAATTCCA CAGTTTTCGC GATCCAGACT GAATGCCCAC AGGCCGTCGA GTTTTTTGAT TTCACGGGTT GGGGTTTCTA CAGGACGGAC GAGTCGTCGG TTCTGTAACT ATCATCATCA TCATAGACAC ACGAAATAAA GTAATCAGAT TATCAGTTAA AGCTATGTAA TATTTACACC ATAACCAATC AATTAAAAAA TAGATCAGTT TAAAGAAAGA TCAAAGCTCA AAAAAATAAA AAGAGAAAAG GGTCCTAACC AAGAAAATGA AGGAGAAAAA CTAGAAATTT ACCCTCAGAT CTACCATGGA CTAGTGATGG AATTCCCGGC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG GATCCATCGA CCTGCAGCCA AGCTTGGCGC GCCGGGCCCA ACATGGTGGA GCACGACACT CTCGTCTACT CCA
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