pBUS1-Pcap-HC-GFP
$113.00
In stock
SKU
PL48948
100μg liquid plasmid(Instantly):$134
| name | pBUS1-Pcap-HC-GFP | another name | apBUS1-Pcap-HC-GFP |
| Insert Size (bp) | Plasmid host | Golden Staphylococcus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Pcap | Replicon | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGA TTGCTGAGTA AAAGATACGA GAGACCTCTC TTGTATCTTT TTTATTTTGA GTGGTTTTGT CCGTTACACT AGAAAACCGA AAGACAATAA AAATTTTATT CTTGCTGAGT CTGGCTTTCG GTAAGCTAGA CAAAACGGAC AAAATAAAAA TTGGCAAGGG TTTAAAGGTG GAGATTTTTT GAGTGATCTT CTCAAAAAAT ACTACCTGTC CCTTGCTGAT TTTTAAACGA GCACGAGAGC AAAACCCCCC TTTGCTGAGG TGGCAGAGGG CAGGTTTTTT TGTTTCTTTT TTCTCGTAAA AAAAAGAAAG GTCTTAAAGG TTTTATGGTT TTGGTCGGCA CTGCCGACAG CCTCGCAGAG CACACACTTT ATGAATATAA AGTATAGTGT GTTATACTTT ACTTGGAAGT GTACAGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGGCC TACGTACCAT TACAGCGGAT CAATTCCCAA TTCCCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGAAT TAATTCCCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG AATTGGGGAT CGGAATTAAT TTTACAAGCC CAGAGCTCCA CCGCGGTGGC GGCCGCTCTA GATTAGTGAT GGTGATGGTG ATGAGAACCT CTCGAGCATG CGGATCCAGC AAAGCTTTAT TTGTATAATT CATCCATACC TAAAGTAATA CCAGCAGCAG TTACGAATTC TAATAATACC ATGTGATCAC GTTTTTCGTT TGGATCTTTT GATAATTTTG ATTGAGTTGA TAAGTAGTGG TTATCTGGTA ATAATACTGG ACCATCACCA ATTGGAGTGT TTTGTTGGTA GTGGTAAGCT AATTGTACAC CACCATCTTC AATGTTGTGA CGAATTTTGA AGTTAGCTTT AATACCGTTA CGTTGTTTAT CAGCCATAAT GTATACGTTG TGTGAGTTGA AGTTGTATTC TAATTTGTGA CCTAAAATGT TACCATCTTC TTTGAAATCA ATACCTTTTA ATTCAATACG GTTTACTAAA GTATCACCTT CGAATTTTAC TTCAGCACGA GTTTTGTAGT TACCATCATC TTTGAAGAAA ATAGTACGTT CTTGAATGTA ACCTTCTGGC ATAGCTGATT TGAAGAAATC GTGTTGTTTC ATGTGATCTG GGTAACGTGA GAAACATTGT ACACCGTAAG TTAAAGTAGT TACTAAAGTT GGCCATGGTA CTGGTAATTT ACCAGTAGTA CAAATGAATT TTAAAGTTAA TTTACCGTAA GTAGCATCAC CTTCACCTTC ACCTGATACT GAGAATTTGT GACCGTTTAC ATCACCATCT AATTCTACTA AAATTGGTAC TACACCAGTG AATAATTCTT CACCTTTTGA TACCATATGA TAAACCTCCT ATTTTCCTTT CTTGTTTTCC ATTATATATA ATCCCCTGTA TATTTTGCAA ACTCTGGTAC CGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGGACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTAGA AAGCACTAAG GAATAATTCC TTAAGGAACG TACAGACGCT TAAAAGCCTT TAAAAACGTT TTTAAGGGGT TTGTAGACAA GGTAAAGGAT AAAACAGCAC AATTCCAAGA AAAACACGAT TTAGAACCTA AAAAGAACGA ATTTGAACTA ACTCATAACC GAGAGGTAAA AAAAGAACGA AGTCGAGATC AGGGAATGAG TTTATAAAAT AAAAAAAGCA CCTGAAAAGG TGTCTTTTTT TGATGGTTTT GAACTTGTTC TTTCTTATCT TGATACATAT AGAAATAACG TCATTTTTAT TTTAGTTGCT GAAAGGTGCG TTGAAGTGTT GGTATGTATG TGTTTTAAAG TATTGGAAAA CCCTTAAAAT TGGTTGCACA GAAAAACCCC ATCTGTTAAA GTTATAAGTG ACCAAACAAA TAACTAAATA GATGGGGGTT TCTTTTAATA TTATGTGTCC TAATAGTAGC ATTTATTCAG ATGAAAAATC AAGGGTTTTA GTGGACAAGA CAAAAAGTGG AAAAGTGAGA CCATGGAGAG AAAAGAAAAT CGCTAATGTT GATTACTTTG AACTTCTGCA TATTCTTGAA TTTAAAAAGG CTGAAAGAGT AAAAGATTGT GCTGAAATAT TAGAGTATAA ACAAAATCGT GAAACAGGCG AAAGAAAGTT GTATCGAGTG TGGTTTTGTA AATCCAGGCT TTGTCCAATG TGCAACTGGA GGAGAGCAAT GAAACATGGC ATTCAGTCAC AAAAGGTTGT TGCTGAAGTT ATTAAACAAA AGCCAACAGT TCGTTGGTTG TTTCTCACAT TAACAGTTAA AAATGTTTAT GATGGCGAAG AATTAAATAA GAGTTTGTCA GATATGGCTC AAGGATTTCG CCGAATGATG CAATATAAAA AAATTAATAA AAATCTTGTT GGTTTTATGC GTGCAACGGA AGTGACAATA AATAATAAAG ATAATTCTTA TAATCAGCAC ATGCATGTAT TGGTATGTGT GGAACCAACT TATTTTAAGA ATACAGAAAA CTACGTGAAT CAAAAACAAT GGATTCAATT TTGGAAAAAG GCAATGAAAT TAGACTATGA TCCAAATGTA AAAGTTCAAA TGATTCGACC GAAAAATAAA TATAAATCGG ATATACAATC GGCAATTGAC GAAACTGCAA AATATCCTGT AAAGGATACG GATTTTATGA CCGATGATGA AGAAAAGAAT TTGAAACGTT TGTCTGATTT GGAGGAAGGT TTACACCGTA AAAGGTTAAT CTCCTATGGT GGTTTGTTAA AAGAAATACA TAAAAAATTA AACCTTGATG ACACAGAAGA AGGCGATTTG ATTCATACAG ATGATGACGA AAAAGCCGAT GAAGATGGAT TTTCTATTAT TGCAATGTGG AATTGGGAAC GGAAAAATTA TTTTATTAAA GAGTAGTTCA ACAAACGGGC CATATTGTTG TATAAGTGAT GAAATACTGA ATTTAAAACT TAGTTTATAT GTGGTAAAAT GTTTTAATCA AGTTTAGGAG GAATTAATTA TGAAGTGTAA TGAATAATGA ATGTAACAGG GTTCAATTAA AAGAGGGAAG CGTATCATTA ACCCTATAAA CTACGTCTGC CCTCATTATT GGAGGGTGAA ATGTGAATAC ATCCTATTCA CAATCGAATT TACGACACAA CCAAATTTTA ATTTGGCTTT GCATTTTATC TTTTTTTAGC GTATTAAATG AAATGGTTTT GAACGTCTCA TTACCTGATA TTGCAAATGA TTTTAATAAA CCACCTGCGA GTACAAACTG GGTGAACACA GCCTTTATGT TAACCTTTTC CATTGGAACA GCTGTATATG GAAAGCTATC TGATCAATTA GGCATCAAAA GGTTACTCCT ATTTGGAATT ATAATAAATT GTTTCGGGTC GGTAATTGGG TTTGTTGGCC ATTCTTTCTT TTCCTTACTT ATTATGGCTC GTTTTATTCA AGGGGCTGGT GCAGCTGCAT TTCCAGCACT CGTAATGGTT GTAGTTGCGC GCTATATTCC AAAGGAAAAT AGGGGTAAAG CATTTGGTCT TATTGGATCG ATAGTAGCCA TGGGAGAAGG AGTCGGTCCA GCGATTGGTG GAATGATAGC CCATTATATT CATTGGTCCT ATCTTCTACT CATTCCTATG ATAACAATTA TCACTGTTCC GTTTCTTATG AAATTATTAA AGAAAGAAGT AAGGATAAAA GGTCATTTTG ATATCAAAGG AATTATACTA ATGTCTGTAG GCATTGTATT TTTTATGTTG TTTACAACAT CATATAGCAT TTCTTTTCTT ATCGTTAGCG TGCTGTCATT CCTGATATTT GTAAAACATA TCAGGAAAGT AACAGATCCT TTTGTTGATC CCGGATTAGG GAAAAATATA CCTTTTATGA TTGGAGTTCT TTGTGGGGGA ATTATATTTG GAACAGTAGC AGGGTTTGTC TCTATGGTTC CTTATATGAT GAAAGATGTT CACCAGCTAA GTACTGCCGA AATCGGAAGT GTAATTATTT TCCCTGGAAC AATGAGTGTC ATTATTTTCG GCTACATTGG TGGGATACTT GTTGATAGAA GAGGTCCTTT ATACGTGTTA AACATCGGAG TTACATTTCT TTCTGTTAGC TTTTTAACTG CTTCCTTTCT TTTAGAAACA ACATCATGGT TCATGACAAT TATAATCGTA TTTGTTTTAG GTGGGCTTTC GTTCACCAAA ACAGTTATAT CAACAATTGT TTCAAGTAGC TTGAAACAGC AGGAAGCTGG TGCTGGAATG AGTTTGCTTA ACTTTACCAG CTTTTTATCA GAGGGAACAG GTATTGCAAT TGTAGGTGGT TTATTATCCA TACCCTTACT TGATCAAAGG TTGTTACCTA TGGAAGTTGA TCAGTCAACT TATCTGTATA GTAATTTGTT ATTACTTTTT TCAGGAATCA TTGTCATTAG TTGGCTGGTT ACCTTGAATG TATATAAACA TTCTCAAAGG GATTTCTAAA TCGTTAAGGG ATCAACTTTG GGAGAGAGTT CAAAATTGAT CCTTTTTTTA TAACAG
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