pAAVS1-MSCV-MCS-EF1a-CopGFP-Puro
$84.00
In stock
SKU
PL48889
100μg liquid plasmid(Instantly):$105
| name | pAAVS1-MSCV-MCS-EF1a-CopGFP-Puro | another name | apAAVS1-MSCV-MCS-EF1a-CopGFP-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | EF1a | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCCTTGGTAC CTGCTTTCTC TGACCAGCAT TCTCTCCCCT GGGCCTGTGC CGCTTTCTGT CTGCAGCTTG TGGCCTGGGT CACCTCTACG GCTGGCCCAG ATCCTTCCCT GCCGCCTCCT TCAGGTTCCG TCTTCCTCCA CTCCCTCTTC CCCTTGCTCT CTGCTGTGTT GCTGCCCAAG GATGCTCTTT CCGGAGCACT TCCTTCTCGG CGCTGCACCA CGTGATGTCC TCTGAGCGGA TCCTCCCCGT GTCTGGGTCC TCTCCGGGCA TCTCTCCTCC CTCACCCAAC CCCATGCCGT CTTCACTCGC TGGGTTCCCT TTTCCTTCTC CTTCTGGGGC CTGTGCCATC TCTCGTTTCT TAGGATGGCC TTCTCCGACG GATGTCTCCC TTGCGTCCCG CCTCCCCTTC TTGTAGGCCT GCATCATCAC CGTTTTTCTG GACAACCCCA AAGTACCCCG TCTCCCTGGC TTTAGCCACC TCTCCATCCT CTTGCTTTCT TTGCCTGGAC ACCCCGTTCT CCTGTGGATT CGGGTCACCT CTCACTCCTT TCATTTGGGC AGCTCCCCTA CCCCCCTTAC CTCTCTAGTC TGTGCTAGCT CTTCCAGCCC CCTGTCATGG CATCTTCCAG GGGTCCGAGA GCTCAGCTAG TCTTCTTCCT CCAACCCGGG CCCCTATGTC CACTTCAGGA CAGCATGTTT GCTGCCTCCA GGGATCCTGT GTCCCCGAGC TGGGACCACC TTATATTCCC AGGGCCGGTT AATGTGGCTC TGGTTCTGGG TACTTTTATC TGTCCCCTCC ACCCCACAGT GGGGCACGCG TAAGGGCGAA TTCTGCAGAT ATCAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC TAGGCGCCGG AATTACCGGT CACGTGATTC GAATTTAAAT CGGATCCCTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGAGATCTGC GGCCGCAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGCTAGACG CCACCATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCAAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGGTGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GTGCTGGTGG GCAGCTTCGC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTTC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGCTGCACA GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCC ATCGCCTTCG CCAGATCCCG CGCTCAGTCG TCCAATTCTG CCGTGGACGG CACCGCCGGA CCCGGCTCCA CCGGATCTCG CGAGGGCAGA GGAAGTCTTC TAACATGCGG TGACGTGGAG GAGAATCCCG GCCCTATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT CTATCAGAAG CTCATCTGGT CTCCCTTCCG GGGGACAAGA CATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCAAACTG GGTTCTTATA TCCCTTGCTC TGGTCAACCA GGTTGCAGGG TTTCCTGTCC TCACAGGAAC GAAGTCCCTA AAGAAACAGT GGCAGCCAGG TTTAGCCCCG GAATTGACTG GATTCCTTTT TTAGGGCCCA TTGGTATGGT GTACACTACT AGGGACAGGA TTGGTGACAG AAAAGCCCCA TCCTTAGGCC TCCTCCTTCC TAGTCTCCTG ATATTGGGTC TAACCCCCAC CTCCTGTTAG GCAGATTCCT TATCTGGTGA CACACCCCCA TTTCCTGGAG CCATCTCTCT CCTTGCCAGA ACCTCTAAGG TTTGCTTACG ATGGAGCCAG AGAGGATCCT GGGAGGGAGA GCTTGGCAGG GGGTGGGAGG GAAGGGGGGG ATGCGTGACC TGCCCGGTTC TCAGTGGCCA CCCTGCGCTA CCCTCTCCCA GAACCTGAGC TGCTCTGACG CGGCTGTCTG GTGCGTTTCA CTGATCCTGG TGCTGCAGCT TCCTTACACT TCCCAAGAGG AGAAGCAGTT TGGAAAAACA AAATCAGAAT AAGTTGGTCC TGAGTTCTAA CTTTGGCTCT TCACCTTTCT AGTCCCCAAT TTATATTGTT CCTCCGTGCG TCAGTTTTAC CTGTGAGATA AGGCCAGTAG CCAGCCCCGT CCTGGCAGGG CTGTGGTGAG GAGGGGGGTG TCCGTGTGGA AAACTCCCTT TGTGAGAATG GTGCGTCCTA GGTGTTCACC AGGTCGTGGC CGCCTCTACT CCCTTTCTCT TTCTCCATCC TTCTTTCCTT AAAGAGTCCC CAGTGCTATC TGGGACATAT TCCTCCGCCC AGAGCAGGGT CCCGCTTCCC TAAGGCCCTG CTCTGGGCTT CTGGGTTTGA GTCCTTGGCA AGCCCAGGAG AGGCGCTCAG GCTTCCCTGT CCCCCTTCCT CGTCCACCAT CTCATGCCCC TGGCTCTCCT GCCCCTTCCC TACAGGTGTT CCTGGCTCTG CTCTCTCGAG ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT AATCTAGCTG CATCAGGATC ATATCGTCGG GTCTTTTTTC CGGCTCAGTC ATCGCCCAAG CTGGCGCTAT CTGGGCATCG GGGAGGAAGA AGCCCGTGCC TTTTCCCGCG AGGTTGAAGC GGCATGGAAA GAGTTTGCCG AGGATGACTG CTGCTGCATT GACGTTGAGC GAAAACGCAC GTTTACCATG ATGATTCGGG AAGGTGTGGC CATGCACGCC TTTAACGGTG AACTGTTCGT TCAGGCCACC TGGGATACCA GTTCGTCGCG GCTTTTCCGG ACACAGTTCC GGATGGTCAG CCCGAAGCGC ATCAGCAACC CGAACAATAC CGGCGACAGC CGGAACTGCC GTGCCGGTGT GCAGATTAAT GACAGCGGTG CGGCGCTGGG ATATTACGTC AGCGAGGACG GGTATCCTGG CTGGATGCCG CAGAAATGGA CATGGATACC CCGTGAGTTA CCCGGCGGGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
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