pCMV-FLAG-CIITA(mouse)-Neo
$99.00
In stock
SKU
PL48595
NM_001302618.1;C2ta; EG669998; Gm9475; Mhc2ta
100μg liquid plasmid(Instantly):$120
| name | pCMV-FLAG-CIITA(mouse)-Neo | another name | apCMV-FLAG-CIITA(mouse)-Neo |
| Insert Size (bp) | 3468bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGCCACC ATGGACTACA AGGACGACGA TGATAAGGGT ACCATGAACC ACTTCCAGGC CATCCTGGCC CAAGTACAGA CACTGCTCTC CAGCCAGAAG CCCAGGCAGG TGCGGGCCCT CCTGGATGGC CTGCTGGAAG AAGAGCTGCT CTCACGGGAA TACCACTGTG CCTTGCTGCA TGAGCCTGAT GGTGATGCCC TGGCCCGGAA GATTTCCCTG ACCCTGCTGG AGAAAGGGGA CTTAGACTTG ACTTTCTTGA GCTGGGTCTG CAACAGTCTG CAGGCTCCCA CGGTAGAGAG GGGCACCAGC TACAGGGACC ATGGAGACCA TAGTCTGTGT GCCACCATGG ATCTGGGATC TCCAGAGGGC AGCTACCTGG AACTCCTTAA CAGTGATGCC GACCCCCTAC ATCTCTACCA CCTCTATGAC CAGATGGACC TGGCTGGGGA GGAGGAGATC GAACTCAGCT CAGAGCCAGA CACAGATACC ATCAACTGCG ACCAGTTCAG CAAGCTGTTG CAGGACATGG AACTGGATGA AGAGACCCGG GAGGCCTATG CCAACATTGC GGAACTGGAT CAGTACGTGT TCCAGGATAC CCAGCTCGAG GGCCTGAGCA AGGACCTCTT CATAGAGCAC ATTGGAGCAG AGGAAGGCTT TGGTGAGAAC ATAGAGATCC CTGTAGAAGC AGGACAGAAG CCTCAGAAGA GACGCTTCCC GGAAGAGCAT GCTATGGACT CAAAGCACAG GAAGCTAGTG CCCACCTCTA GGACCTCACT GAACTATTTG GATCTCCCCA CTGGGCACAT CCAGATCTTC ACCACTCTGC CCCAGGGACT CTGGCAAATC TCAGGGGCTG GCACAGGTCT CTCCAGTGTC CTAATCTACC ACGGTGAGAT GCCCCAGGTC AACCAAGTGC TCCCTTCAAG CAGCCTCAGT ATCCCCAGTC TCCCCGAGTC CCCAGACCGG CCTGGCTCCA CCAGCCCCTT CACACCATCT GCAGCTGACC TGCCCAGCAT GCCCGAACCT GCGCTGACCT CCCGTGTAAA TGAGACAGAG GACACATCTC CCTCCCCATG CCAAGAGGGT CCCGAGTCTT CCATCAAGCT TCCAAAATGG CCAGAGGCTG TGGAGCGATT CCAGCACTCC CTACAGGACA AATACAAGGC ATTGCCCCAG AGCCCAAGGG GTCCTCTGGT GGCCGTGGAG CTGGTACGGG CCAGGCTGGA AAGAGGCAGC AACAAGAGCC AGGAAAGGGA GCTGGCCACT CCCGACTGGA CAGAGCGCCA GCTAGCCCAC GGTGGTCTGG CAGAGGTACT TCAGGTTGTC AGTGACTGCA GGCGACCAGG AGAGACACAG GTGGTCGCTG TGCTGGGCAA GGCTGGCCAG GGAAAGAGCC ACTGGGCCAG GACAGTGAGT CACACCTGGG CATGTGGCCA GTTGCTACAA TATGACTTTG TCTTCTATGT CCCCTGTCAT TGCTTGGATC GTCCCGGGGA CACCTACCAC CTGCGGGATC TGCTCTGTCC CCCGAGCCTG CAGCCACTGG CCATGGATGA CGAGGTCCTT GATTATATCG TGAGGCAGCC AGACCGTGTT CTGCTCATCC TAGATGCTTT CGAGGAGCTA GAGGCCCAAG ATGGCCTCCT GCACGGACCC TGTGGATCTC TGTCCCCAGA GCCCTGCTCC CTCCGAGGAC TGCTGGCTGG GATCTTCCAG CGGAAGCTAC TGCGAGGCTG CACACTGCTC CTCACAGCCC GGCCCCGGGG CCGCCTGGCT CAGAGCCTGA GCAAGGCAGA TGCCATCTTT GAGGTGCCCA GCTTCTCTAC CAAGCAGGCC AAGACTTACA TGAGGCACTA CTTTGAGAAC TCAGGGACAG CGGGGAACCA AGACAAGGCC CTGGGCCTCC TGGAGGGCCA GCCTCTTCTC TGCAGCTATA GTCACAGCCC TGTTGTGTGC AGGGCTGTGT GCCAGCTCTC CAAGGCCCTG CTAGAACAGG GCACAGAGGC CCAGCTACCT TGTACACTTA CAGGACTCTA TGTCAGCCTG CTAGGTCCTG CAGCTCAGAA CAGTCCTCCC GGAGCCTTAG TCGAGCTGGC CAAGCTGGCC TGGGAGCTGG GACGAAGACA CCAAAGCACC TTGCAAGAAA CCCGGTTTTC ATCCGTGGAG GTGAAAACCT GGGCAGTGAC CCAAGGCTTG ATGCAGCAGA CCCTGGAGAC CACGGAGGCT CAACTGGCCT TCTCCAGTTT TCTGCTACAG TGTTTCCTGG GTGCTGTGTG GCTGGCACAG TGCAATGAAA TCAAAGACAA GGAGCTGCCA CAGTACCTGG CCTTGACTCC GAGGAAGAAG AGACCCTATG ACAACTGGCT GGAGGGTGTA CCACGCTTTC TGGCTGGATT AGTTTTCCAG CCTCGAGCCC ACTGCCTGGG AGCTCTGGTG GAGCCTGCAG TGGCTGCAGT GGCGGATAGG AAACAGAAGG TTCTTACCAG GTACCTGAAG CGCCTGAAGC TGGGGACACT CCGGGCAGGG AGGCTGCTGG AGCTGCTCCA CTGTGCCCAC GAGACACAGC AACCTGGGAT ATGGGAGCAT GTTGCACACC AGCTCCCTGG CCACCTCTCC TTCCTGGGCA CCCGGCTCAC ACCCCCAGAT GTGTATGTGC TGGGCAGGGC CTTGGAGACA GCCAGCCAGG ACTTCTCCTT GGACCTTCGT CAGACTGGCG TTGAGCCTTC TGGACTGGGA AACCTCGTGG GACTCAGCTG TGTCACCAGT TTCAGGGCCT CCTTGAGTGA TACAATGGCA TTATGGGAGT CCCTTCAGCA GCAGGGAGAA GCCCAGCTAC TCCAGGCGGC AGAGGAGAAG TTCACCATTG AGCCATTTAA AGCCAAATCC CCAAAGGATG TGGAAGACCT GGATCGTCTC GTGCAGACCC AGAGGCTGAG AAACCCCTCA GAAGATGCAG CCAAGGATCT TCCTGCCATC CGGGACCTTA AGAAGCTAGA GTTTGCGTTG GGCCCCATCT TGGGCCCCCA GGCTTTCCCC ACACTGGCAA AGATCCTTCC AGCCTTCTCT TCTCTGCAAC ACCTGGACCT GGACTCACTT AGTGAGAACA AGATCGGAGA CAAGGGTGTG TCGAAGCTCT CAGCCACCTT CCCTCAGCTG AAGGCCCTGG AGACGCTCAA CTTGTCCCAA AACAACATCA CTGATGTGGG TGCCTGCAAG CTTGCAGAAG CTCTGCCAGC CCTAGCCAAG TCCCTCCTAA GGCTGAGCTT GTACAATAAC TGCATCTGTG ACAAAGGAGC CAAGAGCCTG GCACAAGTAC TTCCGGACAT GGTGTCCCTG CGTGTGATGG ATGTCCAGTT CAACAAGTTC ACGGCTGCCG GTGCCCAGCA ACTGGCCTCC AGCCTTCAGA AGTGCCCTCA GGTGGAAACA CTGGCAATGT GGACACCCAC TATCCCCTTT GGGGTTCAGG AACACCTGCA GCAGCTGGAT GCCAGGATCA GTCTGAGATG AGAATTCTGC AGATATCCAT CACACTGGCG GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT GCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC
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