pY26TEFP-CV23
$113.00
In stock
SKU
PL48389
100μg liquid plasmid(Instantly):$134
| name | pY26TEFP-CV23 | another name | apY26TEFP-CV23 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Multiple frames unloaded | Prokaryotic resistance | Amp |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | TEF1,GAP | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGTATGA TCCAATATCA AAGGAAATGA TAGCATTGAA GGATGAGACT AATCCAATTG AGGAGTGGCA GCATATAGAA CAGCTAAAGG GTAGTGCTGA AGGAAGCATA CGATACCCCG CATGGAATGG GATAATATCA CAGGAGGTAC TAGACTACCT TTCATCCTAC ATAAATAGAC GCATATAAGT ACGCATTTAA GCATAAACAC GCACTATGCC GTTCTTCTCA TGTATATATA TATACAGGCA ACACGCAGAT ATAGGTGCGA CGTGAACAGT GAGCTGTATG TGCGCAGCTC GCGTTGCATT TTCGGAAGCG CTCGTTTTCG GAAACGCTTT GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGAAAGTATA GGAACTTCAG AGCGCTTTTG AAAACCAAAA GCGCTCTGAA GACGCACTTT CAAAAAACCA AAAACGCACC GGACTGTAAC GAGCTACTAA AATATTGCGA ATACCGCTTC CACAAACATT GCTCAAAAGT ATCTCTTTGC TATATATCTC TGTGCTATAT CCCTATATAA CCTACCCATC CACCTTTCGC TCCTTGAACT TGCATCTAAA CTCGACCTCT ACATTTTTTA TGTTTATCTC TAGTATTACT CTTTAGACAA AAAAATTGTA GTAAGAACTA TTCATAGAGT GAATCGAAAA CAATACGAAA ATGTAAACAT TTCCTATACG TAGTATATAG AGACAAAATA GAAGAAACCG TTCATAATTT TCTGACCAAT GAAGAATCAT CAACGCTATC ACTTTCTGTT CACAAAGTAT GCGCAATCCA CATCGGTATA GAATATAATC GGGGATGCCT TTATCTTGAA AAAATGCACC CGCAGCTTCG CTAGTAATCA GTAAACGCGG GAAGTGGAGT CAGGCTTTTT TTATGGAAGA GAAAATAGAC ACCAAAGTAG CCTTCTTCTA ACCTTAACGG ACCTACAGTG CAAAAAGTTA TCAAGAGACT GCATTATAGA GCGCACAAAG GAGAAAAAAA GTAATCTAAG ATGCTTTGTT AGAAAAATAG CGCTCTCGGG ATGCATTTTT GTAGAACAAA AAAGAAGTAT AGATTCTTTG TTGGTAAAAT AGCGCTCTCG CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTTT ACAAAAATGA AGCACAGATT CTTCGTTGGT AAAATAGCGC TTTCGCGTTG CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT GTTCTACAAA ATGAAGCACA GATGCTTCGT TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT ACCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCCT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CGCTATTACG CCAGCTGAAT TGGAGCGACC TCATGCTATA CTGAGAAAGC AACCTGACCT ACAGGAAAGA GTTACTCAAG AATAAGAATT TTCGTTTTAA AACCTAAGAG TCACTTTAAA ATTTGTATAC ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA TTTAGAAGTG TCAACAACGT ATCTACCAAC GATTTGACCC TTTTCCATCT TTTCGTAAAT TTCTGGCAAG GTAGACAAGC CGACAACCTT GATTGGAGAC TTGACCAAAC CTCTGGCGAA GAATTGTTAA TTAAAGATCT CCGCGGTTAT GGCTTCAATG GTGGGTCCTT CAAGTTGAAT TCTCTGAATT GAACGTACAT AGTGATTCTG ATGTTGTAGT CTTGGTCGTA GATAGAGTTT TCGAAAGCAG TACCCAAACC AACGTGGTCA ACGTTACCAG TAGTTTGCAA TCTCAACCAC AATTGGTTTC TCTTGTTGTT TGGTTGGAAG TAGTCGATAG TTCTGTCCAA AACTGGCTTT GGAGTGAAGT ATCTAGAGTG GTAGTAGAAT GGTTGAGTGA TAGTGTGTCT AGAAGAGTAG TTAACGTATG GGTCGTAAGT CAAAGCGTTA GCCTTAGTAA CGAAGTTGTC GTCCAAGATA ACAGCAGTAG AACCAACACC TCTGTCACCT TGAGTGATTG GAGAACATGG CCAGAATTCA ACCTTAACCT TTCTGATTCT GTAGTATTCG AATGGAACAG TCAATGGGTT AGAACCACCA CCTGGTGGCA AGAAGTCGTT GATGTTGAAT CTCATCATGT CAACGTTCCA AGATGGAGTT CTAACAGTAG TCTTCTTAAC AGTGTAACCG ATAGTTCTAG ACAATCTAGT GTTGAAGATA CCGTTTTCAG CCTCTCTCTT CTCGAGAGAT ACCCCTTCTT CTTTAGCAGC AATGCTGGCA ATAGTAGTAT TTATAAACAA TAACCCGTTA TTTGTGCTGT TGGAAAATGG CAAAACAGCA ACATCGAAAT CCCCTTCTAA ATCTGAGTAA CCGATGACAG CTTCAGCCGG AATTTGTGCC GTTTCATCTT CTGTTGTAGT GTTGACTGGA GCAGCTAATG CGGAGGATGC TGCGAATAAA ACAGCAGTAA AAATTGAAGG AAATCTCATA GCGGCCGCGC TAGTTCTAGA AAACTTAGAT TAGATTGCTA TGCTTTCTTT CTAATGAGCA AGAAGTAAAA AAAGTTGTAA TAGAACAAGA AAAATGAAAC TGAAACTTGA GAAATTGAAG ACCGTTTATT AACTTAAATA TCAATGGGAG GTCATCGAAA GAGAAAAAAA TCAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA TTTTTATTGC CTTTTTCGAC GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA ACCTTTAGTA CGGGTAATTA ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC TGTGCTTGGG TGTTTTGAAG TGGTACGGCG ATGCGCGGAG TCCGAGAAAA TCTGGAAGAG TAAAAAAGGA GTAGAAACAT TTTGAAGCTA TGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCCGAGCT CAGTTTATCA TTATCAATAC TCGCCATTTC AAAGAATACG TAAATAATTA ATAGTAGTGA TTTTCCTAAC TTTATTTAGT CAAAAAATTA GCCTTTTAAT TCTGCTGTAA CCCGTACATG CCCAAAATAG GGGGCGGGTT ACACAGAATA TATAACATCG TAGGTGTCTG GGTGAACAGT TTATTCCTGG CATCCACTAA ATATAATGGA GCCCGCTTTT TAAGCTGGCA TCCAGAAAAA AAAAGAATCC CAGCACCAAA ATATTGTTTT CTTCACCAAC CATCAGTTCA TAGGTCCATT CTCTTAGCGC AACTACAGAG AACAGGGGCA CAAACAGGCA AAAAACGGGC ACAACCTCAA TGGAGTGATG CAACCTGCCT GGAGTAAATG ATGACACAAG GCAATTGACC CACGCATGTA TCTATCTCAT TTTCTTACAC CTTCTATTAC CTTCTGCTCT CTCTGATTTG GAAAAAGCTG AAAAAAAAGG TTGAAACCAG TTCCCTGAAA TTATTCCCCT ACTTGACTAA TAAGTATATA AAGACGGTAG GTATTGATTG TAATTCTGTA AATCTATTTC TTAAACTTCT TAAATTCTAC TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATTCTA GAACTAGTAT GAGATTTCCT TCAATTTTTA CTGCTGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAG AGAGAGGCTG AACCAACTGC TGGTACTTAC TACACTAAGA AGTACTCTAC TATGAACGTT ATCTCTGTTG GTACTCCACA AAACAACAAG CCATGGCACG CTAACCACTT CATCACTAGA TTGAACGAAT GGGAAACTGC TATCACTTTC GAATACTACA AGATCTTGAA GATGAAGGTT ACTTTGTCTC CAGTTATCTC TCCAGCTCAA CAAACTAAGA CTATGTTCGG TCACACTGCT ATCGACTTGG ACGGTGCTTG GACTACTAAC ACTTGGTTGC AAGACGACCC ATACGCTGAA TCTTCTACTA GAAAGGTTAT GACTTCTAAG AAGAAGCACT CTAGATACTT CACTCCAAAG CCATTGTTGG CTGGTACTAC TTCTGCTCAC CCAGGTCAAT CTTTGTTCTT CTTCTCTAGA CCAACTCCAT GGTTGAACAC TTACGACCCA ACTGTTCAAT GGGGTGCTTT GTTGTGGTCT ATCTACGTTC CAGAAAAGAC TGGTATGACT GACTTCTACG GTACTAAGGA AGTTTGGATC AGATACAAGT CTTAAGCTTA TCGATACCGT CGACCTCGAG TCATGTAATT AGTTATGTCA CGCTTACATT CACGCCCTCC CCCCACATCC GCTCTAACCG AAAAGGAAGG AGTTAGACAA CCTGAAGTCT AGGTCCCTAT TTATTTTTTT ATAGTTATGT TAGTATTAAG AACGTTATTT ATATTTCAAA TTTTTCTTTT TTTTCTGTAC AGACGCGTGT ACGCATGTAA CATTATACTG AAAACCTTGC TTGAGAAGGT TTTGGGACGC TCGAAGGCTT TAATTTGCGG CCGGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAGGGTAAT AACTGATATA ATTAAATTGA AGCTCTAATT TGTGAGTTTA GTATACATGC ATTTACTTAT AATACAGTTT TTCATGAGAT GCCTGCAAGC AATTCGTTCT GTATCAGGCG CAGGAGCGTC CCGTCCGGGT CGACCAAAGC GGCCATCGTG CCTCCCCACT CCTGCAGTTC GGGGGCATGG ATGCGCGGAT AGCCGCTGCT GGTTTCCTGG ATGCCGACGG ATTTGCACTG CCGGTAGAAC TCCGCGAGGT CGTCCAGCCT CAGGCAGCAG CTGAACCAAC TCGCGAGGGG ATCGAGCCCG GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAATT CGAGCTCGCC CTTAGATTAG ATTGCTATGC TTTCTTTCTA ATGAGCAAGA AGTAAAAAAA GTTGTAATAG AACAAGAAAA ATGAAACTGA AACTTGAGAA ATTGAAGACC GTTTATTAAC TTAAATATCA ATGGGAGGTC ATCGAAAGAG AAAAAAATCA AAAAAAAAAA TTTTCAAGAA AAAGAAACGT GATAAAAATT TTTATTGCCT TTTTCGACGA AGAAAAAGAA ACGAGGCGGT CTCTTTTTTC TTTTCCAAAC CTTTAGTACG GGTAATTAAC GACACCCTAG AGGAAGAAAG AGGGGAAATT TAGTATGCTG TGCTTGGGTG TTTTGAAGTG GTACGGCGAT GCGCGGAGTC CGAGAAAATC TGGAAGAGTA AAAAAGGAGT AGAAACATTT TGAAGCTATG GTGTGTGGCA TATGCGTATA TATACCAATC TAAGTCTGTG CTCCTTCCTT CGTTCTTCCT TCTGTTCGGA GATTACCGAA TCAAAAAAAT TTCAAAGAAA CCGAAATCAA AAAAAAGAAT AAAAAAAAAA TGATGAATTG AATTGAAAAG CTGTGGTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GA
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