pMS2-GST
$99.00
In stock
SKU
PL48074
100μg liquid plasmid(Instantly):$120
| name | pMS2-GST | another name | apMS2-GST |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCTAGAGTCG ACCTGCAGCC CAAGCTTGGG CTGAACATCG AGCGCCAGGG CTCCGTAAAG CTACTAGAGC ACAGGCGGTG CCCCAACGTC CTGGGGCCTC TCCACTAATA ACGGCTACTT CCAATTGATT GGACGCGCCA TCTTGCCTGC CTTATGCATA TTCAGCGGTG AACTGAATAT TCATGAACGA GGCCCGTCCC GTCCCTCCCT CCCTTCCCCC CCCCCCGGAA CCCGCTCCGG AGGACCCGAA GGGCCCCGCC TTCATTACCG ATGCGTAGGA CAAACCATTT TCCCGATGTG TGTGGGGGGA TACTAATGAG AGACTTTAGC TGAAAATGAG CCTGAACTCC GAAGCTGAGT AAAAATGGCC TAACTTTATC CTCCGTTCTG TAAGTCCTCG GTTTGAGTGC ACGGGAAACC CGAAAGGAGG ACGACAGGAC CAGGAACATT CTCCTCCTCC TGTCGCGTCA GAAAGAACAC CCAACCAGGG AGCCGGAGCC CTAGCGTCAA CAACTCCGCC GCGCGCGCTC CGTGTAGGCC GGTGCGGGCG GCCCCGTAGC GCAAGGGAGG GCGGGAAAGG AAGGGGCGGG ACACAAGGGC GAATCTATAA AGGGCGTCAC TCAGCCAGTT CTCTCCTCAG AAGCGCCGAG AGCGCGACCG GGACGGTTGG AGAAGAAGGT GGCTCCCGGA AGGGGGAGAG ACAAACTGCC GTAACCTCTG CCGTTCAGGA TCACTAGTCC ACCATGGGCT ACCCCTACGA CGTGCCCGAC TACGCCATCG AAGGCCGCCA TATGCTAGCC GTTAAAATGG CTTCTAACTT TACTCAGTTC GTTCTCGTCG ACAATGGCGG AACTGGCGAC GTGACTGTCG CCCCAAGCAA CTTCGCTAAC GGGATCGCTG AATGGATCAG CTCTAACTCG CGTTCACAGG CTTACAAAGT AACCTGTAGC GTTCGTCAGA GCTCTGCGCA GAATCGCAAA TACACCATCA AAGTCGAGGT GCCTAAAGGC GCCTGGCGTT CGTACTTAAA TATGGAACTA ACCATTCCAA TTTTCGCCAC GAATTCCGAC TGCGAGCTTA TTGTTAAGGC AATGCAAGGT CTCCTAAAAG ATGGAAACCC GATTCCCTCA GCAATCGCAG CAAACTCCGG CATCTACGCG GATCCACCGG TCGCCACCAT GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AACCAAAAAA GAAGAGAAAG GTATAAATCG ATTCTGCAGT AGCTCGCCGG CAGCTGACTA GCTAGAGCTG AGAACTTCAG GGTGAGTTTG GGGACCCTTG ATTGTTCTTT CTTTTTCGCT ATTGTAAAAT TCATGTTATA TGGAGGGGGC AAAGTTTTCA GGGTGTTGTT TAGAATGGGA AGATGTCCCT TGTATCACCA TGGACCCTCA TGATAATTTT GTTTCTTTCA CTTTCTACTC TGTTGACAAC CATTGTCTCC TCTTATTTTC TTTTCATTTT CTGTAACTTT TTCGTTAAAC TTTAGCTTGC ATTTGTAACG AATTTTTAAA TTCACTTTTG TTTATTTGTC AGATTGTAAG TACTTTCTCT AATCACTTTT TTTTCAAGGC AATCAGGGTA TATTATATTG TACTTCAGCA CAGTTTTAGA GAACAATTGT TATAATTAAA TGATAAGGTA GAATATTTCT GCATATAAAT TCTGGCTGGC GTGGAAATAT TCTTATTGGT AGAAACAACT ACACCCTGGT CATCATCCTG CCTTTCTCTT TATGGTTACA ATGATATACA CTGTTTGAGA TGAGGATAAA ATACTCTGAG TCCAAACCGG GCCCCTCTGC TAACCATGTT CATGCCTTCT TCTCTTTCCT ACAGCTCCTG GGCAACGTGC TGGTTGTTGT GCTGTCTCAT CATTTTGGCA AAGAATTCAC TCCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG CCCTGGCTCA CAAATACCAC TGAGATCTTT TTCCCTCTGC CAAAAATTAT GGGGACATCA TGAAGCCCCT TGAGCATCTG ACTTCTGGCT AATAAAGGAA ATTTATTTTC ATTGCAATAG TGTGTTGGAA TTTTTTGTGT CTCTCACTCG GAAGGACATA TGGGAGGGCA AATCATTTAA AACATCAGAA TGAGTATTTG GTTTAGAGTT TGGCAACATA TGCCCATATG CTGGCTGCCA TGAACAAAGG TTGGCTATAA AGAGGTCATC AGTATATGAA ACAGCCCCCT GCTGTCCATT CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT TTTTATATTT TGTTTTGTGT TATTTTTTTC TTTAACATCC CTAAAATTTT CCTTACATGT TTTACTAGCC AGATTTTTCC TCCTCTCCTG ACTACTCCCA GTCATAGCTG TCCCTCTTCT CTTATGGAGA TCCCTCGAGC GGCCGCGGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGC
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