pHCMC04-xylA-STHA(enterica-opt-bacillus)
$113.00
In stock
SKU
PL47877
100μg liquid plasmid(Instantly):$134
| name | pHCMC04-xylA-STHA(enterica-opt-bacillus) | another name | apHCMC04-xylA-STHA(enterica-opt-bacillus) |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | xylA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGAGGCCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACTGCCGGGC CTCTTGCGGG ATCGGCGAGG CTAGTTACCC TTAAGTTATT GGTATGACTG GTTTTAAGCG CAAAAAAAGT TGCTTTTTCG TACCTATTAA TGTATCGTTT TAGAAAACCG ACTGTAAAAA GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA GAATAAAGAC CACATTAAAA AATGTGGTCT TTTGTGTTTT TTTAAAGGAT TTGAGCGTAG CGAAAAATCC TTTTCTTTCT TATCTTGATA ATAAGGGTAA CTATTGCCGA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCCTAA CTTATAGGGG TAACACTTAA AAAAGAATCA ATAACGATAG AAACCGCTCC TAAAGCAGGT GCATTTTTTC CTAACGAAGA AGGCAATAGT TCACATTTAT TGTCTAAATG AGAATGGACT CTAGAAGAAA CTTCGTTTTT AATCGTATTT AAAACAATGG GATGAGATTC AATTATATGA TTTCTCAAGA TAACAGCTTC TATATCAAAT GTATTAAGGA TATTGGTTAA TCCAATTCCG ATATAAAAGC CAAAGTTTTG AAGTGCATTT AACATTTCTA CATCATTTTT ATTTGCGCGT TCCACAATCT CTTTTCGAGA AATATTCTTT TCTTCTTTAG AGAGCGAAGC CAGTAACGCT TTTTCAGAAG CATATAATTC CCAACAGCCT CGATTTCCAC AGCTGCATTT GGGTCCATTA AAATCTATCG TCATATGACC CATTTCCCCA GAAAAACCCT GAACACCTTT ATACAATTCG TTGTTAATAA CAAGTCCAGT TCCAATTCCG ATATTAATAC TGATGTAAAC GATGTTTTCA TAGTTTTTTG TCATACCAAA TACTTTTTCA CCGTATGCTC CTGCATTAGC TTCATTTTCA ACAAAAACCG GAACATTAAA CTCACTCTCA ATTAAAAACT GCAAATCTTT GATATTCCAA TTTAAGTTAG GCATGAAAAT AATTTGCTGA TGACGATCTA CAAGGCCTGG AACACAAATT CCTATTCCGA CTAGACCATA AGGGGACTCA GGCATATGGG TTACAAAACC ATGAATAAGT GCAAATAAAA TCTCTTTTAC TTCACTAGCG GAAGAACTAG ACAAGTCAGA AGTCTTCTCG AGAATAATAT TTCCTTCTAA GTCGGTTAGA ATTCCGTTAA GATAGTCGAC TCCTATATCA ATACCAATCG AGTAGCCTGC ATTCTTATTA AAAACAAGCA TTACAGGTCT TCTGCCGCCT CTAGATTGCC CTGCCCCAAT TTCAAAAATA AAATCTTTTT CAAGCAGTGT ATTTACTTGA GAGGAGACAG TAGACTTGTT TAATCCTGTA ATCTCAGAGA GAGTTGCCCT GGAGACAGGG GAGTTCTTCA AAATTTCATC TAATATTAAT TTTTGATTCA TTTTTTTTAC TAAAGCTTGA TCTGCAATTT GAATAATAAC CACTCCTTTG TTTATCCACC GAACTAAGTT GGTGTTTTTT GAAGCTTGAA TTAGATATTT AAAAGTATCA TATCTAATAT TATAACTAAA TTTTCTAAAA AAAACATTGA AATAAACATT TATTTTGTAT ATGATGAGAT AAAGTTAGTT TATTGGATAA ACAAACTAAC TCAATTAAGA TAGTTGATGG ATAAACTTGT TCACTTAAAT CAAAGGGGGA AATGACAAAT GGTCCAAACT AGTAAGAGGA GGAGAAATAT GCCCCACAGC TGGGACTATG ATGCCGTTGT CATTGGATCT GGGCCGGGTG GGGAAGGAGC AGCGATGGGA TTGGTCAAAC AGGGCGCAAG AGTCGCTGTC ATAGAACGTT ATCATAACGT AGGTGGAGGC TGCACACATT GGGGGACCAT TCCTTCAAAA GCGCTGCGCC ATGCTGTTTC AAGAATCATT GAATTTAATC AAAATCCGCT TTATTCAGAT CATAGTAGGC TGTTGCGGTC CAGCTTCGCA GACATTCTGA ATCACGCTGA CAATGTGATC AATCAGCAAA CAAGAATGAG ACAGGGATTT TATGAGCGGA ACCATTGTGA GATTTTGCAA GGAAATGCAC ATTTTATTGA TGAGCATACA CTCGCTTTAG AATGCCACGA TGGCACTGTT GAAACCCTGA CGGCCGAAAA ATTCGTCATT GCATGCGGAA GCCGGCCTTA TCACCCAAAC GATGTTGATT TCTCTCATCC GCGCATCTAC GACAGTGACA GCATCCTGAG CCTGCATCAT GAACCACGAC ACGTCATTAT ATATGGCGCC GGAGTGATCG GATGTGAATA CGCATCGATT TTCCGCGGCA TGGATGTGAA AGTGGACTTA ATTAACACGC GTGACCGCCT TTTAGCTTTT TTAGATCAGG AAATGTCCGA TTCACTTTCT TACCACTTTT GGAATTCGGG TGTGGTAATA CGGCACAATG AGGAATATGA GAAAATCGAA GGCTGTGATG ACGGTGTAAT CATGCATCTT AAATCAGGCA AGAAATTGAA GGCTGATTGT TTACTCTACG CTAACGGAAG GACAGGCAAT ACAGATAGTC TTGCATTGGA AAACATAGGG CTTGAAACGG ACAGCCGCGG CCAGCTGAAG GTTAACTCAA TGTATCAAAC CGCCTTGCCA CATGTGTATG CGGTTGGAGA TGTAATTGGT TATCCTTCTC TGGCTTCCGC AGCCTATGAT CAAGGCCGAA TTGCGGCACA GGCGCTTGTT AAAGGAGAGG CGACTGCCCA CCTTATTGAA GATATCCCGA CTGGCATTTA CACGATCCCT GAAATCTCAT CTGTAGGGAA GACAGAACAG CAGCTTACGG CGATGAAAGT GCCGTACGAA GTAGGGCGGG CGCAATTTAA ACATTTAGCC AGAGCTCAAA TCGTGGGAAT GAATGTCGGC ACCTTAAAGA TTTTATTTCA TAGAGAAACA AAAGAGATCT TAGGTATTCA TTGCTTTGGC GAGCGTGCCG CTGAGATCAT ACATATTGGC CAAGCGATTA TGGAACAAAA AGGCGGCGGG AACACAATTG AGTATTTTGT CAATACAACA TTTAATTATC CGACGATGGC AGAAGCATAC CGAGTTGCTG CGCTAAATGG TCTCAACCGT CTCTTCTAAG GATCCTCTAG AGTCGACGTC CCCGGGGCAG CCCGCCTAAT GAGCGGGCTT TTTTCACGTC ACGCGTCCAT GGAGATCTTT GTCTGCAACT GAAAAGTTTA TACCTTACCT GGAACAAATG GTTGAAACAT ACGAGGCTAA TATCGGCTTA TTAGGAATAG TCCCTGTACT AATAAAATCA GGTGGATCAG TTGATCAGTA TATTTTGGAC GAAGCTCGGA AAGAATTTGG AGATGACTTG CTTAATTCCA CAATTAAATT AAGGGAAAGA ATAAAGCGAT TTGATGTTCA AGGAATCACG GAAGAAGATA CTCATGATAA AGAAGCTCTA AAACTATTCA ATAACCTTAC AATGGAATTG ATCGAAAGGG TGGAAGGTTA ATGGTACGAA AATTAGGGGA TCTACCTAGA AAGCCACAAG GCGATAGGTC AAGCTTAAAG AACCCTTACA TGGATCTTAC AGATTCTGAA AGTAAAGAAA CAACAGAGGT TAAACAAACA GAACCAAAAA GAAAAAAAGC ATTGTTGAAA ACAATGAAAG TTGATGTTTC AATCCATAAT AAGATTAAAT CGCTGCACGA AATTCTGGCA GCATCCGAAG GGAATTCATA TTACTTAGAG GATACTATTG AGAGAGCTAT TGATAAGATG GTTGAGACAT TACCTGAGAG CCAAAAAACT TTTTATGAAT ATGAATTAAA AAAAAGAACC AACAAAGGCT GAGACAGACT CCAAACGAGT CTGTTTTTTT AAAAAAAATA TTAGGAGCAT TGAATATATA TTAGAGAATT AAGAAAGACA TGGGAATAAA AATATTTTAA ATCCAGTAAA AATATGATAA GATTATTTCA GAATATGAAG AACTCTGTTT GTTTTTGATG AAAAAACAAA CAAAAAAAAT CCACCTAACG GAATCTCAAT TTAACTAACA GCGGCCAAAC TGAGAAGTTA AATTTGAGAA GGGGAAAAGG CGGATTTATA CTTGTATTTA ACTATCTCCA TTTTAACATT TTATTAAACC CCATACAAGT GAAAATCCTC TTTTACACTG TTCCTTTAGG TGATCGCGGA GGGACATTAT GAGTGAAGTA AACCTAAAAG GAAATACAGA TGAATTAGTG TATTATCGAC AGCAAACCAC TGGAAATAAA ATCGCCAGGA AGAGAATCAA AAAAGGGAAA GAAGAAGTTT ATTATGTTGC TGAAACGGAA GAGAAGATAT GGACAGAAGA GCAAATAAAA AACTTTTCTT TAGACAAATT TGGTACGCAT ATACCTTACA TAGAAGGTCA TTATACAATC TTAAATAATT ACTTCTTTGA TTTTTGGGGC TATTTTTTAG GTGCTGAAGG AATTGCGCTC TATGCTCACC TAACTCGTTA TGCATACGGC AGCAAAGACT TTTGCTTTCC TAGTCTACAA ACAATCGCTA AAAAAATGGA CAAGACTCCT GTTACAGTTA GAGGCTACTT GAAACTGCTT GAAAGGTACG GTTTTATTTG GAAGGTAAAC GTCCGTAATA AAACCAAGGA TAACACAGAG GAATCCCCGA TTTTTAAGAT TAGACGTAAG GTTCCTTTGC TTTCAGAAGA ACTTTTAAAT GGAAACCCTA ATATTGAAAT TCCAGATGAC GAGGAAGCAC ATGTAAAGAA GGCTTTAAAA AAGGAAAAAG AGGGTCTTCC AAAGGTTTTG AAAAAAGAGC ACGATGAATT TGTTAAAAAA ATGATGGATG AGTCAGAAAC AATTAATATT CCAGAGGCCT TACAATATGA CACAATGTAT GAAGATATAC TCAGTAAAGG AGAAATTCGA AAAGAAATCA AAAAACAAAT ACCTAATCCT ACAACATCTT TTGAGAGTAT ATCAATGACA ACTGAAGAGG AAAAAGTCGA CAGTACTTTA AAAAGCGAAA TGCAAAATCG TGTCTCTAAG CCTTCTTTTG ATACCTGGTT TAAAAACACT AAGATCAAAA TTGAAAATAA AAATTGTTTA TTACTTGTAC CGAGTGAATT TGCATTTGAA TGGATTAAGA AAAGATATTT AGAAACAATT AAAACAGTCC TTGAAGAAGC TGGATATGTT TTCGAAAAAA TCGAACTAAG AAAAGTGCAA TAAACTGCTG AAGTATTTCA GCAGTTTTTT TTATTTAGAA ATAGTGAAAA AAATATAATC AGGGAGGTAT CAATATTTAA TGAGTACTGA TTTAAATTTA TTTAGACTGG AATTAATAAT TAACACGTAG ACTAATTAAA ATTTAATGAG GGATAAAGAG GATACAAAAA TATTAATTTC AATCCCTATT AAATTTTAAC AAGGGGGGGA TTAAAATTTA ATTAGAGGTT TATCCACAAG AAAAGACCCT AATAAAATTT TTACTAGGGT TATAACACTG ATTAATTTCT TAATGGGGGA GGGATTAAAA TTTAATGACA AAGAAAACAA TCTTTTAAGA AAAGCTTTTA AAAGATAATA ATAAAAAGAG CTTTGCGATT AAGCAAAACT CTTTACTTTT TCATTGACAT TATCAAATTC ATCGATTTCA AATTGTTGTT GTATCATAAA GTTAATTCTG TTTTGCACAA CCTTTTCAGG AATATAAAAC ACATCTGAGG CTTGTTTTAT AAACTCAGGG TCGCTAAAGT CAATGTAACG TAGCATATGA TATGGTATAG CTTCCACCCA AGTTAGCCTT TCTGCTTCTT CTGAATGTTT TTCATATACT TCCATGGGTA TCTCTAAATG ATTTTCCTCA TGTAGCAAGG TATGAGCAAA AAGTTTATGG AATTGATAGT TCCTCTCTTT TTCTTCAACT TTTTTATCTA AAACAAACAC TTTAACATCT GAGTCAATGT AAGCATAAGA TGTTTTTCCA GTCATAATTT CAATCCCAAA TCTTTTAGAC AGAAATTCTG GACGTAAATC TTTTGGTGAA AGAATTTTTT TATGTAGCAA TATATCCGAT ACAGCACCTT CTAAAAGCGT TGGTGAATAG GGCATTTTAC CTATCTCCTC TCATTTTGTG GAATAAAAAT AGTCATATTC GTCCATCTAC CTATCCTATT ATCGAACAGT TGAACTTTTT AATCAAGGAT CAGTCCTTTT TTTCATTATT CTTAAACTGT GCTCTTAACT TTAACAACTC GATTTGTTTT TCCAGATC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
