pENTR223.1-TPR(human)
$157.00
In stock
SKU
PL47780
NM_003292.3
100μg liquid plasmid(Instantly):$178
| name | pENTR223.1-TPR(human) | another name | apENTR223.1-TPR(human) |
| Insert Size (bp) | 7092bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGGCGGCG GTGTTGCAGC AAGTCCTGGA GCGCACGGAG CTGAACAAGC TGCCCAAGTC TGTCCAGAAC AAACTTGAAA AGTTCCTTGC TGATCAGCAA TCCGAGATCG ATGGCCTGAA GGGGCGGCAT GAGAAATTTA AGGTGGAGAG CGAACAACAG TATTTTGAAA TAGAAAAGAG GTTGTCCCAC AGTCAGGAGA GACTTGTGAA TGAAACCCGA GAGTGTCAAA GCTTGCGGCT TGAGCTAGAG AAACTCAACA ATCAACTGAA GGCACTAACT GAGAAAAACA AAGAACTTGA AATTGCTCAG GATCGCAATA TTGCCATTCA GAGCCAATTT ACAAGAACAA AGGAAGAATT AGAAGCTGAG AAAAGAGACT TAATTAGAAC CAATGAGAGA CTATCTCAAG AACTTGAATA CTTAACAGAG GATGTTAAAC GTCTGAATGA AAAACTTAAA GAAAGCAATA CAACAAAGGG TGAACTTCAG TTAAAATTGG ATGAACTTCA AGCTTCTGAT GTTTCTGTTA AGTATCGAGA AAAACGCTTG GAGCAAGAAA AGGAATTGCT ACATAGTCAG AATACATGGC TGAATACAGA GTTGAAAACC AAAACTGATG AACTTCTGGC TCTTGGAAGA GAAAAAGGGA ATGAGATTCT AGAGCTTAAA TGTAATCTTG AAAATAAAAA AGAAGAGGTT TCTAGACTGG AAGAACAAAT GAATGGCTTA AAAACATCAA ATGAACATCT TCAAAAGCAT GTGGAGGATC TGTTGACCAA ATTAAAAGAG GCCAAGGAAC AACAGGCCAG TATGGAAGAG AAATTCCACA ATGAATTAAA TGCCCACATA AAACTTTCTA ATTTGTACAA GAGTGCCGCT GATGACTCAG AAGCAAAGAG CAATGAACTA ACCCGGGCAG TAGAGGAACT ACACAAACTT TTGAAAGAAG CTGGTGAAGC CAACAAAGCA ATACAAGATC ATCTTCTAGA GGTGGAGCAA TCCAAAGATC AAATGGAAAA AGAAATGCTT GAGAAAATAG GGAGATTGGA GAAGGAATTA GAGAATGCAA ATGACCTTCT TTCTGCCACA AAACGTAAAG GAGCCATATT GTCTGAAGAA GAGCTTGCCG CCATGTCTCC TACTGCAGCA GCTGTAGCTA AGATAGTGAA ACCTGGGATG AAACTAACTG AGCTCTATAA TGCTTATGTG GAAACTCAGG ATCAGTTGCT TTTGGAGAAA CTAGAGAACA AAAGAATTAA TAAGTACCTA GATGAAATAG TGAAAGAAGT GGAAGCCAAA GCACCAATTT TGAAACGCCA GCGTGAGGAA TATGAACGTG CACAGAAAGC TGTAGCAAGT TTATCTGTTA AGCTTGAACA AGCTATGAAG GAGATTCAGC GATTGCAGGA GGACACTGAT AAAGCCAACA AGCAATCATC TGTACTTGAG AGAGATAATC GAAGAATGGA AATACAAGTA AAAGATCTTT CACAACAGAT TAGAGTGCTT TTGATGGAAC TTGAAGAAGC AAGGGGTAAC CACGTAATTC GTGATGAGGA AGTAAGCTCT GCTGATATAA GTAGTTCATC TGAGGTAATA TCACAGCATC TAGTATCTTA CAGAAATATT GAAGAGCTTC AACAACAAAA TCAACGTCTC TTAGTGGCCC TTAGAGAGCT TGGGGAAACC AGAGAAAGAG AAGAACAAGA AACAACTTCA TCCAAAATCA CTGAGCTTCA GCTCAAACTT GAGAGTGCCC TTACTGAACT AGAACAACTC CGCAAATCAC GACAGCATCA AATGCAGCTT GTTGATTCCA TAGTTCGTCA GCGTGATATG TACCGTATTT TATTGTCACA AACAACAGGA GTTGCCATTC CATTACATGC TTCAAGCTTA GATGATGTTT CTCTTGCATC AACTCCAAAA CGTCCAAGTA CATCACAGAC TGTTTCCACT CCTGCTCCAG TACCTGTTAT TGAATCAACA GAGGCTATAG AGGCTAAGGC TGCCCTTAAA CAGTTGCAGG AAATTTTTGA GAACTACAAA AAAGAAAAAG CAGAAAATGA AAAAATACAA AATGAGCAGC TTGAGAAACT TCAAGAACAA GTTACAGATT TGCGATCACA AAATACCAAA ATTTCTACCC AGCTAGATTT TGCTTCTAAA CGTTATGAAA TGCTGCAAGA TAATGTTGAA GGATATCGTC GAGAAATAAC ATCACTTCAT GAGAGAAATC AGAAACTCAC TGCCACAACT CAAAAGCAAG AACAGATTAT CAATACGATG ACTCAAGATT TGAGAGGAGC AAATGAGAAG CTAGCTGTCG CAGAAGTAAG AGCAGAAAAT TTGAAGAAGG AAAAGGAAAT GCTTAAATTG TCTGAAGTTC GTCTTTCTCA GCAAAGAGAG TCTTTGTTAG CTGAACAAAG GGGGCAAAAC TTACTGCTAA CTAATCTGCA AACAATTCAG GGAATACTGG AGCGATCTGA AACAGAAACC AAACAAAGGC TTAGTAGCCA GATAGAAAAA CTGGAACATG AGATCTCTCA TCTAAAGAAG AAGTTGGAAA ATGAGGTGGA ACAAAGGCAT ACACTTACTA GAAATCTAGA TGTTCAACTT TTAGATACAA AGAGACAACT GGATACAGAG ACAAATCTTC ATCTTAACAC AAAAGAACTA TTAAAAAATG CTCAAAAAGA AATTGCCACA TTGAAACAGC ACCTCAGTAA TATGGAAGTC CAAGTTGCTT CTCAGTCTTC ACAGAGAACT GGTAAAGGTC AGCCTAGCAA CAAAGAAGAT GTGGATGATC TTGTGAGTCA GCTAAGACAG ACAGAAGAGC AGGTGAATGA CTTAAAGGAG AGACTCAAAA CAAGTACGAG CAATGTGGAA CAATATCAAG CAATGGTTAC TAGTTTAGAA GAATCCCTGA ACAAGGAAAA ACAGGTGACA GAAGAAGTGC GTAAGAATAT TGAAGTTCGT TTAAAAGAGT CAGCTGAATT TCAGACACAG TTGGAAAAGA AGTTGATGGA AGTAGAGAAG GAAAAACAAG AACTTCAGGA TGATAAAAGA AGAGCCATAG AGAGCATGGA ACAACAGTTA TCTGAATTGA AGAAAACACT TTCTAGTGTT CAGAATGAAG TACAAGAAGC TCTTCAGAGA GCAAGCACAG CTTTAAGTAA TGAGCAGCAA GCCAGACGTG ACTGTCAGGA ACAAGCTAAA ATAGCTGTGG AAGCTCAGAA TAAGTATGAG AGAGAATTGA TGCTGCATGC TGCTGATGTT GAAGCTCTAC AAGCTGCGAA GGAGCAGGTT TCAAAAATGG CATCAGTCCG TCAGCATTTG GAAGAAACAA CACAGAAAGC AGAATCACAG TTGTTGGAGT GTAAAGCATC TTGGGAGGAA AGAGAGAGAA TGTTAAAGGA TGAAGTTTCC AAATGTGTAT GTCGCTGTGA AGATCTGGAG AAACAAAACA GATTACTTCA TGATCAGATC GAAAAATTAA GTGACAAGGT CGTTGCCTCT GTGAAGGAAG GTGTACAAGG TCCACTGAAT GTATCTCTCA GTGAAGAAGG AAAATCTCAA GAACAAATTT TGGAAATTCT CAGATTTATA CGACGAGAAA AAGAAATTGC TGAAACTAGG TTTGAGGTGG CTCAGGTTGA GAGTCTGCGT TATCGACAAA GGGTTGAACT TTTAGAAAGA GAGCTGCAGG AACTGCAAGA TAGTCTAAAT GCTGAAAGGG AGAAAGTCCA GGTAACTGCA AAAACAATGG CTCAGCATGA AGAACTGATG AAGAAAACTG AAACAATGAA TGTAGTTATG GAGACCAATA AAATGCTAAG AGAAGAGAAG GAGAGACTAG AACAGGATCT ACAGCAAATG CAAGCAAAGG TGAGGAAACT GGAGTTAGAT ATTTTACCCT TACAAGAAGC AAATGCTGAG CTGAGTGAGA AAAGCGGTAT GTTGCAGGCA GAGAAGAAGC TCTTAGAAGA GGATGTCAAA CGTTGGAAAG CACGTAACCA GCATCTAGTA AGTCAACAGA AAGATCCAGA TACAGAAGAA TATCGGAAGC TCCTTTCTGA AAAGGAAGTT CATACTAAGC GTATTCAACA ATTGACAGAA GAAATTGGTA GACTTAAAGC TGAAATTGCA AGATCAAATG CATCTTTGAC TAACAACCAG AACTTAATTC AGAGTCTGAA GGAAGATCTA AATAAAGTAA GAACTGAAAA GGAAACCATC CAGAAGGACT TAGATGCCAA AATAATTGAT ATCCAAGAAA AAGTCAAAAC TATTACTCAA GTTAAGAAAA TTGGACGTAG GTACAAGACT CAATATGAAG AACTTAAAGC ACAACAGGAT AAGGTTATGG AGACATCGGC TCAGTCCTCT GGAGACCATC AGGAGCAGCA TGTTTCAGTC CAGGAAATGC AGGAACTCAA AGAAACGCTC AACCAAGCTG AAACAAAATC AAAATCACTT GAAAGTCAAG TAGAGAATCT GCAGAAGACA TTATCTGAAA AAGAGACAGA AGCAAGAAAT CTCCAGGAAC AGACTGTGCA ACTTCAGTCT GAACTTTCAC GACTTCGTCA GGATCTTCAA GATAGAACCA CACAGGAGGA GCAGCTCCGA CAACAGATAA CTGAAAAGGA AGAAAAAACC AGAAAGGCTA TTGTAGCAGC AAAGTCAAAA ATTGCACACT TAGCTGGTGT AAAAGATCAG CTAACTAAAG AAAATGAGGA GCTTAAACAA AGGAATGGAG CCTTAGATCA GCAGAAAGAT GAATTGGATG TTCGCATTAC TGCGCTAAAG TCCCAATATG AAGGTCGAAT TAGTCGCTTG GAAAGAGAAC TCAGGGAGCA TCAAGAGAGA CACCTTGAGC AGAGAGATGA GCCTCAAGAA CCTTCTAATA AGGTCCCTGA ACAGCAGAGA CAGATCACAT TGAAAACAAC TCCAGCTTCT GGTGAAAGAG GAATTGCCAG CACATCAGAC CCACCAACAG CCAATATCAA GCCAACTCCT GTTGTGTCTA CTCCAAGTAA AGTGACAGCT GCAGCTATGG CTGGAAATAA GTCAACACCC AGGGCTAGTA TCCGCCCAAT GGTTACACCT GCAACTGTTA CAAATCCCAC TACTACCCCA ACAGCTACAG TGATGCCCAC TACACAAGTG GAATCACAGG AAGCTATGCA GTCAGAAGGG CCTGTGGAAC ATGTTCCAGT TTTTGGAAGC ACAAGTGGAT CCGTTCGTTC TACTAGTCCT AATGTCCAGC CTTCTATCTC TCAACCTATT TTAACTGTTC AGCAACAAAC ACAGGCTACA GCTTTTGTGC AACCCACTCA ACAGAGTCAT CCTCAGATTG AGCCTGCCAA TCAAGAGTTA TCTTCAAACA TAGTAGAGGT TGTTCAGAGT TCACCAGTTG AGCGGCCTTC TACTTCCACA GCAGTATTTG GCACAGTTTC GGCTACCCCC AGTTCTTCTT TGCCAAAGCG TACACGTGAA GAGGAAGAGG ATAGCACCAT AGAAGCATCA GACCAAGTCT CTGATGATAC AGTGGAAATG CCTCTTCCAA AGAAGTTGAA AAGTGTCACA CCTGTAGGAA CTGAGGAAGA AGTTATGGCA GAAGAAAGTA CTGATGGAGA GGTAGAGACT CAGGTATACA ACCAGGATTC TCAAGATTCC ATTGGAGAAG GAGTTACCCA GGGAGATTAT ACACCTATGG AAGACAGTGA AGAAACCTCT CAGTCTCTAC AAATAGATCT TGGGCCACTT CAATCAGATC AGCAGACGAC AACTTCATCC CAGGATGGTC AAGGCAAAGG AGATGATGTC ATTGTAATTG ACAGTGATGA TGAAGAAGAG GATGATGATG AAAATGATGG AGAACATGAG GATTATGAAG AGGATGAGGA AGATGATGAT GATGATGAAG ATGACACAGG GATGGGAGAT GAGGGTGAAG ATAGTAATGA AGGAACTGGT AGTGCCGATG GCAATGATGG TTATGAAGCT GATGATGCTG AGGGTGGTGA TGGGACTGAT CCAGGTACAG AAACAGAAGA AAGTATGGGT GGAGGTGAAG GTAATCACAG AGCTGCTGAT TCTCAAAACA GTGGTGAAGG AAATACAGGT GCTGCAGAAT CTTCTTTTTC TCAGGAGGTT TCTAGAGAAC AACAGCCATC ATCAGCATCT GAAAGACAGG CCCCTCGAGC ACCTCAGTCA CCGAGACGCC CACCACATCC ACTTCCCCCA AGACTGACCA TTCATGCCCC ACCTCAGGAG TTGGGACCAC CAGTTCAGAG AATTCAGATG ACCCGAAGGC AGTCTGTAGG ACGTGGCCTT CAGTTGACTC CAGGAATAGG TGGCATGCAA CAGCATTTTT TTGATGATGA AGACAGAACA GTTCCAAGTA CTCCAACTCT TGTGGTGCCA CATCGTACTG ATGGATTTGC TGAAGCAATT CATTCGCCGC AGGTTGCTGG TGTCCCTAGA TTCCGGTTTG GGCCACCTGA AGATATGCCA CAAACAAGTT CTAGTCACTC TGATCTTGGC CAGCTTGCTT CTCAAGGAGG TTTAGGAATG TATGAAACAC CCCTGTTCCT AGCTCATGAA GAAGAGTCAG GTGGCCGAAG TGTTCCCACT ACTCCACTAC AAGTAGCAGC CCCAGTGACT GTATTTACTG AGAGCACCAC CTCTGATGCT TCGGAACATG CCTCTCAATC TGTTCCAATG GTGACTACAT CCACTGGCAC TTTATCTACA ACAAATGAAA CAGCAACAGG TGATGATGGA GATGAAGTAT TTGTGGAGGC AGAATCTGAA GGTATTAGTT CAGAAGCAGG CCTAGAAATT GATAGCCAGC AGGAAGAAGA GCCGGTTCAA GCATCTGATG AGTCAGATCT CCCCTCCACC AGCCAGGATC CTCCTTCTAG CTCATCTGTA GATACTAGTA GTAGTCAACC AAAGCCTTTC AGACGAGTAA GACTTCAGAC AACATTGAGA CAAGGTGTCC GTGGTCGTCA GTTTAACAGA CAGAGAGGTG TGAGCCATGC AATGGGAGGG AGAGGAGGAA TAAACAGAGG AAATATTAAT TCAGGCCTCA TGGGCCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAAAAA TAATTGCTCA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGCCTC CTCTAGACCA GCCAGGACAG AAATGCCTCG ACTTCGCTGC TGCCCAAGGT TGCCGGGTGA CGCACACCGT GGAAACGGAT GAAGGCACGA ACCCAGTGGA CATAAGCCTG TTCGGTTCGT AAGCTGTAAT GCAAGTAGCG TATGCGCTCA CGCAACTGGT CCAGAACCTT GACCGAACGC AGCGGTGGTA ACGGCGCAGT GGCGGTTTTC ATGGCTTGTT ATGACTGTTT TTTTGGGGTA CAGTCTATGC CTCGGGCATC CAAGCAGCAA GCGCGTTACG CCGTGGGTCG ATGTTTGATG TTATGGAGCA GCAACGATGT TACGCAGCAG GGCAGTCGCC CTAAAACAAA GTTAAACATC ATGAGGGAAG CGGTGATCGC CGAAGTATCG ACTCAACTAT CAGAGGTAGT TGGCGTCATC GAGCGCCATC TCGAACCGAC GTTGCTGGCC GTACATTTGT ACGGCTCCGC AGTGGATGGC GGCCTGAAGC CACACAGTGA TATTGATTTG CTGGTTACGG TGACCGTAAG GCTTGATGAA ACAACGCGGC GAGCTTTGAT CAACGACCTT TTGGAAACTT CGGCTTCCCC TGGAGAGAGC GAGATTCTCC GCGCTGTAGA AGTCACCATT GTTGTGCACG ACGACATCAT TCCGTGGCGT TATCCAGCTA AGCGCGAACT GCAATTTGGA GAATGGCAGC GCAATGACAT TCTTGCAGGT ATCTTCGAGC CAGCCACGAT CGACATTGAT CTGGCTATCT TGCTGACAAA AGCAAGAGAA CATAGCGTTG CCTTGGTAGG TCCAGCGGCG GAGGAACTCT TTGATCCGGT TCCTGAACAG GATCTATTTG AGGCGCTAAA TGAAACCTTA ACGCTATGGA ACTCGCCGCC CGACTGGGCT GGCGATGAGC GAAATGTAGT GCTTACGTTG TCCCGCATTT GGTACAGCGC AGTAACCGGC AAAATCGCGC CGAAGGATGT CGCTGCCGAC TGGGCAATGG AGCGCCTGCC GGCCCAGTAT CAGCCCGTCA TACTTGAAGC TAGACAGGCT TATCTTGGAC AAGAAGAAGA TCGCTTGGCC TCGCGCGCAG ATCAGTTGGA AGAATTTGTC CACTACGTGA AAGGCGAGAT CACCAAGGTA GTCGGCAAAT AACCCTCGAG CCACCCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAA
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