NM_000426.4(1point mutation);LAMM; MDC1A
100μg liquid plasmid(Instantly):$207
| name | pENTR223.1-LAMA2(human)(1point mutation) | another name | apENTR223.1-LAMA2(human)(1point mutation) |
| Insert Size (bp) | 9369bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGCCGGGA GCCGCCGGGG TCCTCCTCCT TCTGCTGCTC TCCGGAGGCC TCGGGGGCGT ACAGGCGCAG CGGCCGCAGC AGCAGCGGCA GTCACAGGCA CATCAGCAAA GAGGTTTATT CCCTGCTGTC CTGAATCTTG CTTCTAATGC TCTTATCACG ACCAATGCAA CATGTGGAGA AAAAGGACCT GAAATGTACT GCAAATTGGT AGAACATGTC CCTGGGCAGC CTGTGAGGAA CCCGCAGTGT CGAATCTGCA ATCAAAACAG CAGCAATCCA AACCAGAGAC ACCCGATTAC AAATGCTATT GATGGAAAGA ACACTTGGTG GCAGAGTCCC AGTATTAAGA ATGGAATCGA ATACCATTAT GTGACAATTA CCCTGGATTT ACAGCAGGTG TTCCAGATCG CGTATGTGAT TGTGAAGGCA GCTAACTCCC CCCGGCCTGG AAACTGGATT TTGGAACGCT CTCTTGATGA TGTTGAATAC AAGCCCTGGC AGTATCATGC TGTGACAGAC ACGGAGTGCC TAACGCTTTA CAATATTTAT CCCCGCACTG GGCCACCGTC ATATGCCAAA GATGATGAGG TCATCTGCAC TTCATTTTAC TCCAAGATAC ACCCCTTAGA AAATGGAGAG ATTCACATCT CTTTAATCAA TGGGAGACCA AGTGCCGATG ATCCTTCTCC AGAACTGCTA GAATTTACCT CCGCTCGCTA TATTCGCCTG AGATTTCAGA GGATCCGCAC ACTGAATGCT GACTTGATGA TGTTTGCTCA CAAAGACCCA AGAGAAATTG ACCCCATTGT CACCAGAAGA TATTACTACT CGGTCAAGGA TATTTCAGTT GGAGGGATGT GCATCTGCTA TGGTCATGCC AGGGCTTGTC CACTTGATCC AGCGACAAAT AAATCTCGCT GTGAGTGTGA GCATAACACA TGTGGCGATA GCTGTGATCA GTGCTGTCCA GGATTCCATC AGAAACCCTG GAGAGCTGGA ACTTTTCTAA CTAAAACTGA ATGTGAAGCA TGCAATTGTC ATGGAAAAGC TGAAGAATGC TATTATGATG AAAATGTTGC CAGAAGAAAT CTGAGTTTGA ATATACGTGG AAAGTACATT GGAGGGGGTG TCTGCATTAA TTGTACCCAA AACACTGCTG GTATAAACTG CGAGACATGT ACTGATGGCT TCTTCAGACC CAAAGGGGTA TCTCCAAATT ATCCAAGGCC ATGCCAGCCA TGTCATTGCG ATCCAATTGG TTCCTTAAAT GAAGTCTGTG TCAAGGATGA GAAACATGCT CGACGAGGTT TGGCACCTGG ATCCTGTCAT TGCAAAACTG GTTTTGGAGG TGTGAGCTGT GATCGGTGTG CCAGGGGCTA CACTGGCTAC CCGGACTGCA AAGCCTGTAA CTGCAGTGGG TTAGGGAGCA AAAATGAGGA TCCTTGTTTT GGCCCCTGTA TCTGCAAGGA AAATGTTGAA GGAGGAGACT GTAGTCGTTG CAAATCCGGC TTCTTCAATT TGCAAGAGGA TAATTGGAAA GGCTGCGATG AGTGTTTCTG TTCAGGGGTT TCAAACAGAT GTCAGAGTTC CTACTGGACC TATGGCAAAA TACAAGATAT GAGTGGCTGG TATCTGACTG ACCTTCCTGG CCGCATTCGA GTGGCTCCCC AGCAGGACGA CTTGGACTCA CCTCAGCAGA TCAGCATCAG TAACGCGGAG GCCCGGCAAG CCCTGCCGCA CAGCTACTAC TGGAGCGCGC CGGCTCCCTA TCTGGGAAAC AAACTCCCAG CAGTAGGAGG ACAGTTGACA TTTACCATAT CATATGACCT TGAAGAAGAG GAAGAAGATA CAGAACGTGT TCTCCAGCTT ATGATTATCT TAGAGGGTAA TGACTTGAGC ATCAGCACAG CCCAAGATGA GGTGTACCTG CACCCATCTG AAGAACATAC TAATGTATTG TTACTTAAAG AAGAATCATT TACCATACAT GGCACACATT TTCCAGTCCG TAGAAAGGAA TTTATGACAG TGCTTGCGAA TTTGAAGAGA GTCCTCCTAC AAATCACATA CAGCTTTGGG ATGGATGCCA TCTTCAGGTT GAGCTCTGTT AACCTTGAAT CCGCTGTCTC CTATCCTACT GATGGAAGCA TTGCAGCAGC TGTAGAAGTG TGTCAGTGCC CACCAGGGTA TACTGGCTCC TCTTGTGAAT CTTGTTGGCC TAGGCACAGG CGAGTTAACG GCACTATTTT TGGTGGCATC TGTGAGCCAT GTCAGTGCTT TGGTCATGCG GAGTCCTGTG ATGACGTCAC TGGAGAATGC CTGAACTGTA AGGATCACAC AGGTGGCCCA TATTGTGATA AATGTCTTCC TGGTTTCTAT GGCGAGCCTA CTAAAGGAAC CTCTGAAGAC TGTCAACCCT GTGCCTGTCC ACTCAATATC CCATCCAATA ACTTTAGCCC AACGTGCCAT TTAGACCGGA GTCTTGGATT GATCTGTGAT GGATGCCCTG TCGGGTACAC AGGACCACGC TGTGAGAGGT GTGCAGAAGG CTATTTTGGA CAACCCTCTG TACCTGGAGG ATCATGTCAG CCATGCCAAT GCAATGACAA CCTTGACTTC TCCATCCCTG GCAGCTGTGA CAGCTTGTCT GGCTCCTGTC TGATATGTAA ACCAGGTACA ACAGGCCGGT ACTGTGAGCT CTGTGCTGAT GGATATTTTG GAGATGCAGT TGATGCGAAG AACTGTCAGC CCTGTCGCTG TAATGCCGGT GGCTCTTTCT CTGAGGTTTG CCACAGTCAA ACTGGACAGT GTGAGTGCAG AGCCAACGTT CAGGGTCAGA GATGTGACAA ATGCAAGGCT GGGACCTTTG GCCTACAATC AGCAAGGGGC TGTGTTCCCT GCAACTGCAA TTCTTTTGGG TCTAAGTCAT TCGACTGTGA AGAGAGTGGA CAATGTTGGT GCCAACCTGG AGTCACAGGG AAGAAATGTG ACCGCTGTGC CCACGGCTAT TTCAACTTCC AAGAAGGAGG CTGCACAGCT TGTGAATGTT CTCATCTGGG TAATAATTGT GACCCAAAGA CTGGGCGATG CATTTGCCCT CCCAATACCA TTGGAGAGAA ATGTTCTAAA TGTGCACCCA ATACCTGGGG CCACAGCATT ACCACTGGTT GTAAGGCTTG TAACTGCAGC ACAGTGGGAT CCTTGGATTT CCAATGCAAT GTAAATACAG GCCAATGCAA CTGTCATCCA AAATTCTCTG GTGCAAAATG TACAGAGTGC AGTCGAGGTC ACTGGAACTA CCCTCGCTGC AATCTCTGTG ACTGCTTCCT CCCTGGGACA GATGCCACAA CCTGTGATTC AGAGACTAAA AAATGCTCCT GTAGTGATCA AACTGGGCAG TGCACTTGTA AGGTGAATGT GGAAGGCATC CACTGTGACA GATGCCGGCC TGGCAAATTC GGACTCGATG CCAAGAATCC ACTTGGCTGC AGCAGCTGCT ATTGCTTCGG CACTACTACC CAGTGCTCTG AAGCAAAAGG ACTGATCCGG ACGTGGGTGA CTCTGAAGGC TGAGCAGACC ATTCTACCCC TGGTAGATGA GGCTCTGCAG CACACGACCA CCAAGGGCAT TGTTTTTCAA CATCCAGAGA TTGTTGCCCA CATGGACCTG ATGAGAGAAG ATCTCCATTT GGAACCTTTT TATTGGAAAC TTCCAGAACA ATTTGAAGGA AAGAAGTTGA TGGCCTATGG GGGCAAACTC AAGTATGCAA TCTATTTCGA GGCTCGGGAA GAAACAGGTT TCTCTACATA TAATCCTCAA GTGATCATTC GAGGTGGGAC ACCTACTCAT GCTAGAATTA TCGTCAGGCA TATGGCTGCT CCTCTGATTG GCCAATTGAC AAGGCATGAA ATTGAAATGA CAGAGAAAGA ATGGAAATAT TATGGGGATG ATCCTCGAGT CCATAGAACT GTGACCCGAG AAGACTTCTT GGATATACTA TATGATATTC ATTACATTCT TATCAAAGCT ACTTATGGAA ATTTCATGCG ACAAAGCAGG ATTTCTGAAA TCTCAATGGA GGTAGCTGAA CAAGGACGTG GAACAACAAT GACTCCTCCA GCTGACTTGA TTGAAAAATG TGATTGTCCC CTGGGCTATT CTGGCCTGTC CTGTGAGGCA TGCTTGCCGG GATTTTATCG ACTGCGTTCT CAACCAGGTG GCCGCACCCC TGGACCAACC CTGGGCACCT GTGTTCCATG TCAATGTAAT GGACACAGCA GCCTGTGTGA CCCTGAAACA TCGATATGCC AGAATTGTCA ACATCACACT GCTGGTGACT TCTGTGAACG ATGTGCTCTT GGATACTATG GAATTGTCAA GGGATTGCCA AATGACTGTC AGCAATGTGC CTGCCCTCTG ATTTCTTCCA GTAACAATTT CAGCCCCTCT TGTGTCGCAG AAGGACTTGA CGACTACCGC TGCACGGCTT GTCCACGGGG ATATGAAGGC CAGTACTGTG AAAGGTGTGC CCCTGGCTAT ACTGGCAGTC CAGGCAACCC TGGAGGCTCC TGCCAAGAAT GTGAGTGTGA TCCCTATGGC TCACTGCCTG TGCCCTGTGA CCCTGTCACA GGATTCTGCA CGTGCCGACC TGGAGCCACG GGAAGGAAGT GTGACGGCTG CAAGCACTGG CATGCACGCG AGGGCTGGGA GTGTGTTTTT TGTGGAGATG AGTGCACTGG CCTTCTTCTC GGTGACTTGG CTCGCCTGGA GCAGATGGTC ATGAGCATCA ACCTCACTGG TCCGCTGCCT GCGCCATATA AAATGCTGTA TGGTCTTGAA AATATGACTC AGGAGCTAAA GCACTTGCTG TCACCTCAGC GGGCCCCAGA GAGGCTTATT CAGCTGGCAG AGGGCAATCT GAATACACTC GTGACCGAAA TGAACGAGCT GCTGACCAGG GCTACCAAAG TGACAGCAGA TGGCGAGCAG ACCGGACAGG ATGCTGAGAG GACCAACACA AGAGCAAAGT CCCTGGGAGA ATTCATTAAG GAGCTTGCCC GGGATGCAGA AGCTGTAAAT GAAAAAGCTA TAAAACTAAA TGAAACTCTA GGAACTCGAG ACGAGGCCTT TGAGAGAAAT TTGGAAGGGC TTCAGAAAGA GATTGACCAG ATGATTAAAG AACTGAGGAG GAAAAATCTA GAGACACAAA AGGAAATTGC TGAAGATGAG TTGGTAGCTG CAGAAGCCCT TCTGAAAAAA GTGAAGAAGC TGTTTGGAGA GTCCCGGGGG GAAAATGAAG AAATGGAGAA GGATCTCCGG GAAAAACTGG CTGACTACAA AAACAAAGTT GATGATGCTT GGGACCTTTT GAGAGAAGCC ACAGATAAAA TCAGAGAAGC TAATCGCCTA TTTGCAGTAA ATCAGAAAAA CATGACTGCA TTGGAGAAAA AGAAGGAGGC TGTTGAAAGC GGCAAACGAC AAATTGAGAA CACTTTAAAA GAGGGCAATG ACATACTCGA TGAAGCCAAC CGTCTTGCAG ATGAAATCAA CTCCATCATA GACTATGTTG AAGACATCCA AACTAAATTG CCACCTATGT CTGAGGAGCT TAATGATAAA ATAGATGACC TCTCCCAAGA AATAAAGGAC AGGAAGCTTG CTGAGAAGGT GTCCCAGGCT GAGAGCCACG CAGCTCAGTT GAATGACTCA TCTGCTGTCC TTGATGGAAT CCTTGATGAG GCTAAAAACA TCTCCTTCAA TGCCACTGCA GCCTTCAAAG CTTACAGCAA TATTAAGGAC TATATTGATG AAGCTGAGAA AGTTGCCAAA GAAGCCAAAG ATCTTGCACA TGAAGCTACA AAACTGGCAA CAGGTCCTCG GGGTTTATTA AAGGAAGATG CCAAAGGCTG TCTTCAGAAA AGCTTCAGGA TTCTTAACGA AGCCAAGAAG TTAGCAAATG ATGTAAAAGA AAATGAAGAC CATCTAAATG GCTTAAAAAC CAGGATAGAA AATGCTGATG CTAGAAATGG GGATCTCTTG AGAACTTTGA ATGACACTTT GGGAAAGTTA TCAGCTATTC CAAATGATAC AGCTGCTAAA CTGCAAGCTG TTAAGGACAA AGCCAGACAA GCCAACGACA CAGCTAAAGA TGTACTGGCA CAGATTACAG AGCTCCACCA GAACCTCGAT GGCCTGAAGA AGAATTACAA TAAACTAGCA GACAGCGTCG CCAAAACGAA TGCTGTGGTT AAAGATCCTT CCAAGAACAA AATCATTGCC GATGCAGATG CCACTGTCAA AAATTTAGAA CAGGAAGCTG ACCGGCTAAT AGATAAACTC AAACCCATCA AGGAACTTGA GGATAACCTA AAGAAAAACA TCTCTGAGAT AAAGGAATTG ATAAACCAAG CTCGGAAACA AGCCAATTCT ATCAAAGTAT CTGTGTCTTC AGGAGGTGAC TGCATTCGAA CATACAAACC AGAAATCAAG AAAGGAAGTT ACAATAATAT TGTTGTCAAC GTAAAGACAG CTGTTGCTGA TAACCTCCTC TTTTATCTTG GAAGTGCCAA ATTTATTGAC TTTCTGGCTA TAGAAATGCG TAAAGGCAAA GTCAGCTTCC TCTGGGATGT TGGATCTGGA GTTGGACGTG TAGAGTACCC AGATTTGACT ATTGATGACT CATATTGGTA CCGTATCGTA GCATCAAGAA CTGGGAGAAA TGGAACTATT TCTGTGAGAG CCCTGGATGG ACCCAAAGCC AGCATTGTGC CCAGCACACA CCATTCGACG TCTCCTCCAG GGTACACGAT TCTAGATGTG GATGCAAATG CAATGCTGTT TGTTGGTGGC CTGACTGGGA AATTAAAGAA GGCTGATGCT GTACGTGTGA TTACATTCAC TGGCTGCATG GGAGAAACAT ACTTTGACAA CAAACCTATA GGTTTGTGGA ATTTCCGAGA AAAAGAAGGT GACTGCAAAG GATGCACTGT CAGTCCTCAG GTGGAAGATA GTGAGGGGAC TATTCAATTT GATGGAGAAG GTTATGCATT GGTCAGCCGT CCCATTCGCT GGTACCCCAA CATCTCCACT GTCATGTTCA AGTTCAGAAC ATTTTCTTCG AGTGCTCTTC TGATGTATCT TGCCACACGA GACCTGAGAG ATTTCATGAG TGTGGAGCTC ACTGATGGGC ACATAAAAGT CAGTTACGAT CTGGGCTCAG GAATGGCTTC CGTTGTCAGC AATCAAAACC ATAATGATGG GAAATGGAAA TCATTCACTC TGTCAAGAAT TCAAAAACAA GCCAATATAT CAATTGTAGA TATAGATACT AATCAGGAGG AGAATATAGC AACTTCGTCT TCTGGAAACA ACTTTGGTCT TGACTTGAAA GCAGATGACA AAATATATTT TGGTGGCCTG CCAACGCTGA GAAACTTGAG TATGAAAGCA AGGCCAGAAG TAAATCTGAA GAAATATTCC GGCTGCCTCA AAGATATTGA AATTTCAAGA ACTCCGTACA ATATACTCAG TAGTCCCGAT TATGTTGGTG TTACCAAAGG ATGTTCCCTG GAGAATGTTT ACACAGTTAG CTTTCCTAAG CCTGGTTTTG TGGAGCTCTC CCCTGTGCCA ATTGATGTAG GAACAGAAAT CAACCTGTCA TTCAGCACCA AGAATGAGTC CGGCATCATT CTTTTGGGAA GTGGAGGGAC ACCAGCACCA CCTAGGAGAA AACGAAGGCA GACTGGACAG GCCTATTATG CAATACTCCT CAACAGGGGC CGTCTGGAAG TGCATCTCTC CACAGGGGCA CGAACAATGA GGAAAATTGT GATCAGACCA GAGCCGAATC TGTTTCATGA TGGAAGAGAA CATTCCGTTC ATGTAGAGCG AACTAGAGGC ATCTTTACAG TTCAAGTGGA TGAAAACAGA AGATACATGC AAAACCTGAC AGTTGAACAG CCTATCGAAG TTAAAAAGCT TTTCGTTGGG GGTGCTCCAC CTGAATTTCA ACCTTCCCCA CTCAGAAATA TTCCTCCTTT TGAAGGCTGC ATATGGAATC TTGTTATTAA CTCTGTCCCC ATGGACTTTG CAAGGCCTGT GTCCTTCAAA AATGCTGACA TTGGTCGCTG TGCCCATCAG AAACTCCGTG AAGATGAAGA TGGAGCAGCT CCAGCTGAAA TAGTTATCCA GCCTGAGCCA GTTCCCACCC CAGCCTTTCC TACGCCCACC CCAGTTCTGA CACATGGTCC TTGTGCTGCA GAATCAGAAC CAGCTCTTTT GATAGGGAGC AAGCAGTTCG GGCTTTCAAG AAACAGTCAC ATTGCAATTG CATTTGATGA CACCAAAGTT AAAAACCGTC TCACAATTGA GTTGGAAGTA AGAACCGAAG CTGAATCCGG CTTGCTTTTT TACATGGCTC GCATCAATCA TGCTGATTTT GCAACAGTTC AGCTGAGAAA TGGATTGCCC TACTTCAGCT ATGACTTGGG GAGTGGGGAC ACCCACACCA TGATCCCCAC CAAAATCAAT GATGGCCAGT GGCACAAGAT TAAGATAATG AGAAGTAAGC AAGAAGGAAT TCTTTATGTA GATGGGGCTT CCAACAGAAC CATCAGTCCC AAAAAAGCCG ACATCCTGGA TGTCGTGGGA ATGCTGTATG TTGGTGGGTT ACCCATCAAC TACACTACCC GAAGAATTGG TCCAGTGACC TATAGCATTG ATGGCTGCGT CAGGAATCTC CACATGGCAG AGGCCCCTGC CGATCTGGAA CAACCCACCT CCAGCTTCCA TGTTGGGACA TGTTTTGCAA ATGCTCAGAG GGGAACATAT TTTGACGGAA CCGGTTTTGC CAAAGCAGTT GGTGGATTCA AAGTGGGATT GGACCTTCTT GTAGAATTTG AATTCCGCAC AACTACAACG ACTGGAGTTC TTCTGGGGAT CAGTAGTCAA AAAATGGATG GAATGGGTAT TGAAATGATT GATGAAAAGT TGATGTTTCA TGTGGACAAT GGTGCGGGCA GATTCACTGC TGTCTATGAT GCTGGGGTTC CAGGGCATTT GTGTGATGGA CAATGGCATA AAGTCACTGC CAACAAGATC AAACACCGCA TTGAGCTCAC AGTCGATGGG AACCAGGTGG AAGCCCAAAG CCCAAACCCA GCATCTACAT CAGCTGACAC AAATGACCCT GTGTTTGTTG GAGGCTTCCC AGATGACCTC AAGCAGTTTG GCCTAACAAC CAGTATTCCG TTCCGAGGTT GCATCAGATC CCTGAAGCTC ACCAAAGGCA CAGGCAAGCC ACTGGAGGTT AATTTTGCCA AGGCCCTGGA ACTGAGGGGC GTTCAACCTG TATCATGCCC AGCCAACTCA GGCCTCATGG GCCCAGCTTT CTTGTACAAA GTTGGCATTA TAAAAAATAA TTGCTCATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC ATGCCTCCTC TAGACCAGCC AGGACAGAAA TGCCTCGACT TCGCTGCTGC CCAAGGTTGC CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTGGACAT AAGCCTGTTC GGTTCGTAAG CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT CAACTATCAG AGGTAGTTGG CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA CATTTGTACG GCTCCGCAGT GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG GTTACGGTGA CCGTAAGGCT TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG GAAACTTCGG CTTCCCCTGG AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT GTGCACGACG ACATCATTCC GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA TGGCAGCGCA ATGACATTCT TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG GCTATCTTGC TGACAAAAGC AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG GAACTCTTTG ATCCGGTTCC TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG CTATGGAACT CGCCGCCCGA CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC CGCATTTGGT ACAGCGCAGT AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG GCAATGGAGC GCCTGCCGGC CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT CTTGGACAAG AAGAAGATCG CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC TACGTGAAAG GCGAGATCAC CAAGGTAGTC GGCAAATAAC CCTCGAGCCA CCCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAA
