NM_015089.4(1synonymous mutations );H7AP1; PARC
100μg liquid plasmid(Instantly):$178
| name | pENTR223.1-CUL9(human)(1synonymous mutations) | another name | apENTR223.1-CUL9(human)(1synonymous mutations) |
| Insert Size (bp) | 7554bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGGTGGGG GAACGGCATG CTGGGGACCT CATGGTGCCC TTAGGGCCTC GGCTGCAGGC ATATCCTGAA GAACTCATTC GACAGAGGCC TGGGCATGAC GGGCATCCTG AATACCTGAT CCGATGGAGT GTCCTGAAGT GTGGGGAAGT GGGCAAAGTG GGTGTGGAAG AAGGCAAAGC AGAGCACATC CTCATGTGGC TGTCGGCTCC TGAGGTCTAC GCCAACTGCC CTGGGCTGTT AGGTGAGCGG GCACTATCTA AGGGACTTCA GCACGAACCA GCTGGGGTTT CAGGAAGCTT TCCTCGAGAT CCAGGAGGCC TGGATGAAGT GGCAATGGGA GAGATGGAGG CTGATGTTCA GGCGCTGGTA CGCAGGGCGG CCAGGCAGCT GGCAGAAAGT GGGACCCCAA GCCTCACGGC CGCTGTGCTT CACACCATCC ACGTGCTCAG TGCCTACGCC AGCATCGGGC CCCTCACTGG TGTCTTCAGG GAGACAGGAG CCCTGGACCT GCTCATGCAC ATGTTATGCA ATCCTGAGCC TCAGATCCGC CGGAGTGCAG GCAAAATGCT GCAGGCTCTG GCAGCCCACG ATGCTGGGAG TCGGGCTCAC GTCCTTCTAT CACTGAGCCA GCAAGATGGC ATCGAGCAGC ACATGGATTT TGACAGTCGC TATACATTGC TGGAGCTGTT TGCAGAAACC ACATCCTCTG AAGAACACTG CATGGCCTTT GAGGGCATTC ATCTGCCTCA GATCCCAGGA AAGCTGCTTT TCTCCTTGGT GAAGCGCTAC CTTTGTGTCA CGTCCCTCCT GGATCAGCTG AATAGCAGTC CAGAGCTGGG AGCTGGAGAC CAAAGCTCCC CATGTGCCAC AAGAGAGAAA AGCCGGGGAC AGCGGGAACT GGAGTTCAGC ATGGCTGTGG GCAACCTCAT CTCTGAGCTT GTGCGGAGCA TGGGCTGGGC CCGGAACCTC AGCGAACAGG GCATGTCACC TCCCCGGCCA ACCCGGTCCA TCTTTCAGCC CTACATTTCA GGCCCCAGCC TTTTACTCCC CACCATTGTC ACCACCCCCA GAAGACAAGG GTGGGTCTTC CGCCAGCGCT CTGAATTCTC CAGCCGTAGT GGCTATGGAG AATATGTGCA GCAGACACTG CAGCCAGGGA TGCGAGTGCG GATGCTGGAT GATTATGAGG AGATCAGTGC TGGGGACGAG GGCGAGTTCC GGCAGAGCAA CAACGGCATT CCCCCTGTGC AGGTTTTCTG GCAGTCGACA GGCCGCACTT ACTGGGTGCA CTGGCACATG CTGGAGATCC TGGGCCCTGA GGAAGCCACT GAGGATAAGG CTTCAGCAGC TGTGGAGAAG GGGGCAGGGG CTACTGTGTT GGGCACAGCA TTTCCCTCCT GGGACTGGAA TCCTATGGAT GGGCTGTACC CTTTGCCGTA CCTCCAGCCC GAACCTCAGA AGAATGAGAG AGTGGGATAT CTGACCCAGG CTGAATGGTG GGAGCTGCTT TTCTTTATCA AAAAGTTGGA CTTGTGTGAG CAGCAGCCAA TTTTCCAGAA TCTTTGGAAG AACCTGGATG AGACCCTGGG TGAAAAGGCC CTAGGTGAGA TCTCTGTGTC CGTGGAAATG GCCGAGAGTC TGCTGCAGGT TCTCAGTAGT CGATTTGAGG GCAGCACTCT CAATGACCTG CTCAACTCCC AGATCTACAC CAAGTATGGG CTGCTGTCTA ATGAACCAAG CAGCTCGTCT ACTTCACGAA ATCACTCCTG TACCCCAGAT CCAGAAGAGG AGTCCAAGTC GGAGGCCAGC TTCTCAGAGG AAGAGACTGA GTCCCTCAAA GCAAAGGCCG AGGCCCCTAA GACAGAGGCC GAGCCCACCA AGACAAGGAC CGAGACCCCC ATGGCACAGA GTGATTCTCA GCTGTTTAAC CAGCTTCTGG TGACTGAGGG GATGACCCTG CCCACTGAGA TGAAGGAGGC AGCCAGTGAA ATGGCCAGAG CCTTGCGGGG TCCCGGTCCT CGCAGCTCCC TGGATCAGCA TGTGGCAGCG GTCGTGGCCA CTGTGCAGAT ATCCAGCTTG GACACAAACC TGCAGCTTTC AGGGCTCTCT GCCCTCTCTC AGGCTGTGGA GGAGGTCACT GAGCGGGACC ACCCTCTGGT CCGTCCTGAC AGATCGCTGA GAGAGAAGCT AGTGAAGATG CTGGTGGAGC TGCTGACCAA CCAGGTGGGA GAGAAGATGG TGGTCGTGCA GGCCCTGCGC CTCCTTTACC TGCTCATGAC CAAGCACGAG TGGCGGCCGC TCTTTGCCAG GGAGGGTGGC ATCTATGCTG TGCTGGTCTG CATGCAAGAA TATAAGACTT CTGTCTTGGT GCAGCAGGCT GGGCTGGCGG CACTGAAGAT GCTGGCCGTC GCCAGCTCCT CGGAGATCCC CACTTTTGTT ACTGGCCGAG ATTCTATCCA CTCTTTGTTT GATGCTCAGA TGACCAGAGA GATCTTCGCC AGCATCGACT CAGCCACACG CCCGGGCTCT GAGAGCCTGC TCCTCACTGT CCCTGCAGCC GTGATCCTGA TGCTGAATAC TGAGGGGTGC TCTTCTGCAG CGAGAAATGG CTTACTCCTG CTCAACCTAC TTTTGTGCAA CCACCACACT CTGGGAGACC AGATTATAAC CCAAGAGCTG AGAGACACGT TGTTTAGGCA CTCAGGGATA GCACCAAGAA CAGAACCTAT GCCTACCACA CGCACCATCC TCATGATGCT TCTCAATCGC TACTCAGAGC CGCCGGGCAG CCCTGAGCGT GCAGCACTAG AGACCCCCAT CATCCAGGGT CAGGATGGGT CCCCTGAGCT ACTGATTCGA TCCCTGGTTG GGGGCCCATC TGCAGAACTA CTCCTGGACT TGGAGCGTGT GCTGTGCCGT GAGGGCAGCC CCGGAGGTGC CGTGAGGCCC CTCCTCAAGC GCCTCCAGCA GGAGACCCAG CCTTTCCTCC TGTTGCTGCG GACTCTGGAT GCTCCGGGGC CCAACAAGAC TCTGCTGCTG TCTGTGCTGA GGGTCATAAC CCGACTGCTG GATTTCCCTG AGGCAATGGT CCTCCCCTGG CACGAGGTCT TGGAGCCCTG CCTCAACTGC CTGAGTGGCC CTAGCAGTGA CTCCGAGATT GTTCAGGAGC TGACCTGCTT CCTACATCGC CTGGCCTCGA TGCATAAGGA CTATGCTGTG GTGCTCTGCT GCCTGGGAGC AAAAGAGATC CTCTCCAAAG TCCTGGACAA GCACTCAGCT CAGCTGCTGC TGGGCTGTGA GCTTCGGGAC CTGGTGACAG AGTGTGAGAA GTACGCACAG CTCTATAGCA ACCTCACCTC CAGCATCCTG GCCGGCTGCA TTCAGATGGT GCTGGGCCAG ATCGAAGACC ACAGACGAAC CCACCAACCC ATCAATATCC CCTTCTTTGA TGTGTTCCTC AGGCATCTCT GCCAGGGCTC CAGTGTGGAA GTGAAGGAGG ACAAGTGCTG GGAGAAGGTG GAGGTGTCCT CCAACCCGCA CCGAGCCAGC AAGCTGACGG ACCACAACCC CAAGACCTAC TGGGAGTCCA ACGGCAGCAC CGGCTCCCAC TACATCACCC TGCACATGCA CCGTGGTGTT CTTGTTAGGC AGCTCACTTT GCTGGTGGCC AGTGAGGACT CAAGCTACAT GCCAGCCAGG GTGGTGGTGT TTGGGGGTGA CAGCACCAGC TGCATCGGCA CTGAGCTCAA CACGGTGAAT GTGATGCCCT CTGCCAGCCG GGTGATCCTC TTGGAGAACC TGAACCGCTT CTGGCCCATC ATCCAGATCC GCATAAAGCG CTGCCAGCAG GGCGGCATTG ACACCCGGGT TCGGGGTGTG GAGGTCCTGG GCCCTAAGCC CACATTCTGG CCACTGTTCC GGGAGCAGCT GTGTCGCCGA ACATGTCTCT TCTACACAAT TCGGGCACAA GCCTGGAGCC GGGACATAGC AGAGGACCAC CGGCGCCTCC TCCAGCTCTG TCCCAGACTG AACAGGGTTT TGCGCCACGA GCAGAATTTT GCTGACCGCT TCCTCCCTGA TGATGAGGCC GCCCAGGCAC TGGGCAAGAC CTGCTGGGAG GCCCTGGTCA GCCCCCTGGT GCAGAACATC ACCTCTCCCG ATGCGGAAGG CGTGAGTGCC CTGGGATGGC TGCTGGATCA GTACTTAGAA CAGAGAGAGA CCTCTCGGAA CCCCTTGAGT CGAGCAGCGT CCTTTGCTTC TCGAGTTCGT CGCCTTTGCC ACTTGCTGGT GCATGTGGAA CCTCCTCCTG GGCCTTCTCC TGAGCCATCC ACTCGGCCCT TCAGCAAGAA CAGCAAGGGT CGGGACCGGA GCCCGGCGCC TTCGCCAGTG CTTCCAAGCA GCAGCCTGAG GAACATAACC CAGTGCTGGC TGAGCGTGGT GCAGGAGCAG GTCAGCAGAT TCCTGGCTGC AGCTTGGAGG GCCCCAGACT TTGTGCCTCG TTACTGTAAA CTCTATGAGC ACTTGCAGAG AGCAGGCTCC GAGCTGTTTG GGCCTCGGGC AGCCTTCATG CTGGCTCTGC GCAGTGGCTT CTCTGGCGCC TTGCTGCAGC AGTCCTTCCT CACTGCTGCT CACATGAGTG AGCAGTTTGC CAGGTACATT GACCAACAGA TCCAGGGTGG CCTGATTGGT GGAGCCCCTG GAGTGGAAAT GCTGGGGCAG CTTCAGCGGC ACCTGGAACC CATTATGGTC CTTTCTGGTC TGGAACTGGC CACAACTTTT GAGCACTTCT ATCAGCATTA TATGGCGGAC CGTCTCCTGA GCTTTGGTTC GAGCTGGCTG GAGGGGGCTG TGCTAGAGCA GATTGGCCTC TGTTTTCCCA ACCGCCTCCC ACAGCTGATG CTGCAGAGCC TGAGCACCTC TGAGGAGCTG CAGCGCCAGT TCCACCTCTT CCAGCTCCAG CGGCTCGACA AGTTGTTCTT GGAGCAGGAA GATGAGGAGG AAAAGAGACT AGAGGAAGAG GAGGAGGAAG AGGAGGAAGA GGAAGCTGAG AAAGAATTAT TTATCGAAGA TCCAAGTCCA GCCATTTCTA TACTGGTCCT GTCACCACGC TGCTGGCCCG TCTCCCCACT CTGCTACCTG TACCATCCCA GAAAGTGCCT TCCCACAGAA TTCTGTGATG CCCTTGACCG TTTCTCCAGT TTCTACAGCC AGAGTCAGAA CCATCCAGTC CTGGACATGG GACCACATCG GCGACTGCAG TGGACGTGGC TGGGCCGGGC TGAGCTGCAG TTTGGGAAGC AGATACTGCA TGTGTCCACC GTGCAGATGT GGCTGCTGCT GAAATTCAAT CAGACAGAGG AGGTGTCAGT AGAGACCTTG CTGAAGGATT CTGACCTCTC CCCAGAGCTG CTGCTCCAGG CACTCGTGCC CCTCACCTCA GGGAATGGCC CTTTGACCCT GCATGAGGGC CAGGACTTTC CACACGGGGG TGTGCTGCGG CTTCATGAGC CTGGGCCCCA GCGCAGTGGG GAGGCCCTGT GGCTGATACC TCCCCAGGCA TACCTGAACG TAGAGAAGGA TGAAGGCCGA ACCCTGGAAC AGAAGAGGAA TCTCTTGAGC TGTCTTCTTG TTCGTATTCT CAAAGCCCAT GGGGAAAAGG GCCTCCACAT TGATCAGCTG GTTTGTCTGG TGCTGGAGGC CTGGCAGAAG GGTCCAAATC CTCCTGGAAC CCTGGGCCAC ACTGTTGCTG GGGGTGTGGC CTGTACCAGT ACAGATGTCC TCTCTTGCAT CCTGCACCTC TTAGGCCAGG GCTACGTGAA ACGGCGTGAT GACCGGCCCC AGATCCTGAT GTATGCCGCT CCAGAGCCCA TGGGGCCCTG CCGGGGTCAG GCAGATGTCC CTTTCTGTGG CAGCCAGAGC GAAACCTCCA AGCCCAGCCC AGAAGCTGTG GCTACCCTGG CATCTCTACA GCTGCCTGCA GGCCGCACCA TGAGCCCCCA GGAAGTAGAA GGGTTGATGA AGCAGACGGT GCGTCAGGTG CAGGAGACGC TGAACTTAGA GCCAGATGTC GCTCAGCACC TTTTGGCTCA TTCCCACTGG GGCGCTGAAC AGCTGCTGCA GAGCTACAGT GAGGACCCTG AGCCACTGCT GCTGGCAGCT GGGCTGTGCG TACACCAGGC TCAGGCTGTA CCCGTACGGC CTGACCACTG CCCCGTCTGT GTGAGCCCCC TGGGGTGTGA CGACGACCTG CCCTCTCTCT GCTGCATGCA CTATTGCTGT AAGTCTTGCT GGAATGAGTA CCTGACAACT CGGATCGAGC AGAACCTTGT TTTGAATTGC ACCTGCCCCA TTGCCGACTG CCCCGCCCAG CCCACCGGAG CCTTCATTCG TGCCATCGTC TCCTCGCCAG AGGTCATCTC CAAGTATGAG AAGGCGCTCC TGCGTGGCTA TGTGGAGAGC TGCTCCAACC TGACCTGGTG CACCAACCCC CAGGGCTGCG ACCGCATCCT GTGCCGCCAG GGCCTGGGCT GTGGGACCAC CTGCTCCAAG TGTGGCTGGG CCTCTTGCTT CAACTGTAGC TTCCCTGAGG CACACTACCC TGCTAGCTGT GGCCATATGT CTCAGTGGGT CGACGACGGT GGCTACTATG ACGGCATGAG CGTGGAGGCG CAGAGCAAGC ACCTGGCCAA GCTCATCTCC AAGCGCTGTC CCAGCTGTCA GGCTCCCATC GAGAAGAACG AGGGGTGCCT GCACATGACC TGTGCCAAAT GTAACCATGG ATTCTGCTGG CGCTGCCTCA AGTCCTGGAA GCCAAATCAC AAAGACTATT ACAACTGCTC TGCCATGGTA AGCAAGGCAG CTCGCCAGGA GAAGCGGTTT CAGGACTATA ATGAGAGGTG CACTTTCCAT CACCAGGCGC GGGAGTTTGC TGTGAACTTG CGGAACCGGG TGTCTGCCAT CCATGAAGTG CCCCCGCCCA GATCCTTCAC CTTCCTCAAT GATGCCTGCC AGGGACTGGA GCAGGCTCGG AAGGTGCTGG CCTACGCCTG CGTGTACAGC TTCTACAGCC AGGACGCAGA GTACATGGAT GTGGTGGAGC AGCAGACAGA GAACCTGGAG CTGCACACCA ATGCCCTGCA GATCCTCCTG GAGGAAACCC TGCTGCGGTG CAGAGACCTG GCCTCCTCCC TGCGCCTCCT GCGGGCCGAC TGCCTCAGCA CGGGCATGGA GCTGCTCCGG CGGATCCAGG AGAGGCTGCT TGCCATCCTG CAGCATTCTG CCCAGGATTT CCGGGTTGGT CTTCAGAGTC CATCAGTAGA GGCCTGGGAG GCAAAAGGAC CCAACATGCC TGGCAGTCAG CCCCAGGCCT CCTCAGGGCC AGAGGCAGAA GAGGAGGAGG AAGACGATGA GGATGATGTG CCCGAGTGGC AGCAGGATGA GTTTGATGAG GAGCTGGACA ATGACAGCTT CTCCTACGAT GAGTCTGAGA ACCTGGACCA AGAGACTTTC TTCTTTGGTG ATGAGGAAGA GGATGAAGAT GAGGCCTATG ACTCAGGCCT CATGGGCCCA GCTTTCTTGT ACAAAGTTGG CATTATAAAA AATAATTGCT CATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGCC TCCTCTAGAC CAGCCAGGAC AGAAATGCCT CGACTTCGCT GCTGCCCAAG GTTGCCGGGT GACGCACACC GTGGAAACGG ATGAAGGCAC GAACCCAGTG GACATAAGCC TGTTCGGTTC GTAAGCTGTA ATGCAAGTAG CGTATGCGCT CACGCAACTG GTCCAGAACC TTGACCGAAC GCAGCGGTGG TAACGGCGCA GTGGCGGTTT TCATGGCTTG TTATGACTGT TTTTTTGGGG TACAGTCTAT GCCTCGGGCA TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT GTTACGCAGC AGGGCAGTCG CCCTAAAACA AAGTTAAACA TCATGAGGGA AGCGGTGATC GCCGAAGTAT CGACTCAACT ATCAGAGGTA GTTGGCGTCA TCGAGCGCCA TCTCGAACCG ACGTTGCTGG CCGTACATTT GTACGGCTCC GCAGTGGATG GCGGCCTGAA GCCACACAGT GATATTGATT TGCTGGTTAC GGTGACCGTA AGGCTTGATG AAACAACGCG GCGAGCTTTG ATCAACGACC TTTTGGAAAC TTCGGCTTCC CCTGGAGAGA GCGAGATTCT CCGCGCTGTA GAAGTCACCA TTGTTGTGCA CGACGACATC ATTCCGTGGC GTTATCCAGC TAAGCGCGAA CTGCAATTTG GAGAATGGCA GCGCAATGAC ATTCTTGCAG GTATCTTCGA GCCAGCCACG ATCGACATTG ATCTGGCTAT CTTGCTGACA AAAGCAAGAG AACATAGCGT TGCCTTGGTA GGTCCAGCGG CGGAGGAACT CTTTGATCCG GTTCCTGAAC AGGATCTATT TGAGGCGCTA AATGAAACCT TAACGCTATG GAACTCGCCG CCCGACTGGG CTGGCGATGA GCGAAATGTA GTGCTTACGT TGTCCCGCAT TTGGTACAGC GCAGTAACCG GCAAAATCGC GCCGAAGGAT GTCGCTGCCG ACTGGGCAAT GGAGCGCCTG CCGGCCCAGT ATCAGCCCGT CATACTTGAA GCTAGACAGG CTTATCTTGG ACAAGAAGAA GATCGCTTGG CCTCGCGCGC AGATCAGTTG GAAGAATTTG TCCACTACGT GAAAGGCGAG ATCACCAAGG TAGTCGGCAA ATAACCCTCG AGCCACCCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
