NM_004448.3;CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU;NGL; TKR1
100μg liquid plasmid(Instantly):$120
| name | pLV3-EF1a-ERBB2(human)-3×FLAG-BSD | another name | apLV3-EF1a-ERBB2(human)-3×FLAG-BSD |
| Insert Size (bp) | 3768bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Blast | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG ACATGGATTG GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA GCTCGATACA TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC AGACCTGGAG GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA GTAAAAATTG AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC ACTATGGGCG CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA GTGCAGCAGC AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC AGGCCCGAAG GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA GTGAACGGAT CTCGACGGTA TCGGTTAACT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA AAACAAATTA CAAAATTCAA AATTTTATCG ATGCCTCCCC GTCACCACCC CCCCCAACCC GCCCCGACCG GAGCTGAGAG TAATTCATAC AAAAGGACTC GCCCCTGCCT TGGGGAATCC CAGGGACCGT CGTTAAACTC CCACTAACGT AGAACCCAGA GATCGCTGCG TTCCCGCCCC CTCACCCGCC CGCTCTCGTC ATCACTGAGG TGGAGAAGAG CATGCGTGAG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACGCC CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAAAT TTTTGATGAT ATCCTGCGAC GCTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCTGCACA CTGGTATTTC GGTTTTTGGG GCCGCGGGCG GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGGCCGGCC TGCTCTGGTG CCTGGCCTCG CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC CCGGTCGGCA CCAGTTGCGT GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG CTCAAAATGG AGGACGCGGC GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA AAGGGCCTTT CCGTCCTCAG CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC CAGGCACCTC GATTAGTTCT CGAGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAAAACTACC CCTCTAGATA ATACGACTCA CTATAGGGCC CGGGTTGGAT CCGCCACCAT GGAGCTGGCG GCCTTGTGCC GCTGGGGGCT CCTCCTCGCC CTCTTGCCCC CCGGAGCCGC GAGCACCCAA GTGTGCACCG GCACAGACAT GAAGCTGCGG CTCCCTGCCA GTCCCGAGAC CCACCTGGAC ATGCTCCGCC ACCTCTACCA GGGCTGCCAG GTGGTGCAGG GAAACCTGGA ACTCACCTAC CTGCCCACCA ATGCCAGCCT GTCCTTCCTG CAGGATATCC AGGAGGTGCA GGGCTACGTG CTCATCGCTC ACAACCAAGT GAGGCAGGTC CCACTGCAGA GGCTGCGGAT TGTGCGAGGC ACCCAGCTCT TTGAGGACAA CTATGCCCTG GCCGTGCTAG ACAATGGAGA CCCGCTGAAC AATACCACCC CTGTCACAGG GGCCTCCCCA GGAGGCCTGC GGGAGCTGCA GCTTCGAAGC CTCACAGAGA TCTTGAAAGG AGGGGTCTTG ATCCAGCGGA ACCCCCAGCT CTGCTACCAG GACACGATTT TGTGGAAGGA CATCTTCCAC AAGAACAACC AGCTGGCTCT CACACTGATA GACACCAACC GCTCTCGGGC CTGCCACCCC TGTTCTCCGA TGTGTAAGGG CTCCCGCTGC TGGGGAGAGA GTTCTGAGGA TTGTCAGAGC CTGACGCGCA CTGTCTGTGC CGGTGGCTGT GCCCGCTGCA AGGGGCCACT GCCCACTGAC TGCTGCCATG AGCAGTGTGC TGCCGGCTGC ACGGGCCCCA AGCACTCTGA CTGCCTGGCC TGCCTCCACT TCAACCACAG TGGCATCTGT GAGCTGCACT GCCCAGCCCT GGTCACCTAC AACACAGACA CGTTTGAGTC CATGCCCAAT CCCGAGGGCC GGTATACATT CGGCGCCAGC TGTGTGACTG CCTGTCCCTA CAACTACCTT TCTACGGACG TGGGATCCTG CACCCTCGTC TGCCCCCTGC ACAACCAAGA GGTGACAGCA GAGGATGGAA CACAGCGGTG TGAGAAGTGC AGCAAGCCCT GTGCCCGAGT GTGCTATGGT CTGGGCATGG AGCACTTGCG AGAGGTGAGG GCAGTTACCA GTGCCAATAT CCAGGAGTTT GCTGGCTGCA AGAAGATCTT TGGGAGCCTG GCATTTCTGC CGGAGAGCTT TGATGGGGAC CCAGCCTCCA ACACTGCCCC GCTCCAGCCA GAGCAGCTCC AAGTGTTTGA GACTCTGGAA GAGATCACAG GTTACCTATA CATCTCAGCA TGGCCGGACA GCCTGCCTGA CCTCAGCGTC TTCCAGAACC TGCAAGTAAT CCGGGGACGA ATTCTGCACA ATGGCGCCTA CTCGCTGACC CTGCAAGGGC TGGGCATCAG CTGGCTGGGG CTGCGCTCAC TGAGGGAACT GGGCAGTGGA CTGGCCCTCA TCCACCATAA CACCCACCTC TGCTTCGTGC ACACGGTGCC CTGGGACCAG CTCTTTCGGA ACCCGCACCA AGCTCTGCTC CACACTGCCA ACCGGCCAGA GGACGAGTGT GTGGGCGAGG GCCTGGCCTG CCACCAGCTG TGCGCCCGAG GGCACTGCTG GGGTCCAGGG CCCACCCAGT GTGTCAACTG CAGCCAGTTC CTTCGGGGCC AGGAGTGCGT GGAGGAATGC CGAGTACTGC AGGGGCTCCC CAGGGAGTAT GTGAATGCCA GGCACTGTTT GCCGTGCCAC CCTGAGTGTC AGCCCCAGAA TGGCTCAGTG ACCTGTTTTG GACCGGAGGC TGACCAGTGT GTGGCCTGTG CCCACTATAA GGACCCTCCC TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG AAACCTGACC TCTCCTACAT GCCCATCTGG AAGTTTCCAG ATGAGGAGGG CGCATGCCAG CCTTGCCCCA TCAACTGCAC CCACTCCTGT GTGGACCTGG ATGACAAGGG CTGCCCCGCC GAGCAGAGAG CCAGCCCTCT GACGTCCATC ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC GTGGTCTTGG GGGTGGTCTT TGGGATCCTC ATCAAGCGAC GGCAGCAGAA GATCCGGAAG TACACGATGC GGAGACTGCT GCAGGAAACG GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA GCGATGCCCA ACCAGGCGCA GATGCGGATC CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG GTGCTTGGAT CTGGCGCTTT TGGCACAGTC TACAAGGGCA TCTGGATCCC TGATGGGGAG AATGTGAAAA TTCCAGTGGC CATCAAAGTG TTGAGGGAAA ACACATCCCC CAAAGCCAAC AAAGAAATCT TAGACGAAGC ATACGTGATG GCTGGTGTGG GCTCCCCATA TGTCTCCCGC CTTCTGGGCA TCTGCCTGAC ATCCACGGTG CAGCTGGTGA CACAGCTTAT GCCCTATGGC TGCCTCTTAG ACCATGTCCG GGAAAACCGC GGACGCCTGG GCTCCCAGGA CCTGCTGAAC TGGTGTATGC AGATTGCCAA GGGGATGAGC TACCTGGAGG ATGTGCGGCT CGTACACAGG GACTTGGCCG CTCGGAACGT GCTGGTCAAG AGTCCCAACC ATGTCAAAAT TACAGACTTC GGGCTGGCTC GGCTGCTGGA CATTGACGAG ACAGAGTACC ATGCAGATGG GGGCAAGGTG CCCATCAAGT GGATGGCGCT GGAGTCCATT CTCCGCCGGC GGTTCACCCA CCAGAGTGAT GTGTGGAGTT ATGGTGTGAC TGTGTGGGAG CTGATGACTT TTGGGGCCAA ACCTTACGAT GGGATCCCAG CCCGGGAGAT CCCTGACCTG CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC CCCATCTGCA CCATTGATGT CTACATGATC ATGGTCAAAT GTTGGATGAT TGACTCTGAA TGTCGGCCAA GATTCCGGGA GTTGGTGTCT GAATTCTCCC GCATGGCCAG GGACCCCCAG CGCTTTGTGG TCATCCAGAA TGAGGACTTG GGCCCAGCCA GTCCCTTGGA CAGCACCTTC TACCGCTCAC TGCTGGAGGA CGATGACATG GGGGACCTGG TGGATGCTGA GGAGTATCTG GTACCCCAGC AGGGCTTCTT CTGTCCAGAC CCTGCCCCGG GCGCTGGGGG CATGGTCCAC CACAGGCACC GCAGCTCATC TACCAGGAGT GGCGGTGGGG ACCTGACACT AGGGCTGGAG CCCTCTGAAG AGGAGGCCCC CAGGTCTCCA CTGGCACCCT CCGAAGGGGC TGGCTCCGAT GTATTTGATG GTGACCTGGG AATGGGGGCA GCCAAGGGGC TGCAAAGCCT CCCCACACAT GACCCCAGCC CTCTACAGCG GTACAGTGAG GACCCCACAG TACCCCTGCC CTCTGAGACT GATGGCTACG TTGCCCCCCT GACCTGCAGC CCCCAGCCTG AATATGTGAA CCAGCCAGAT GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT GCCACTCTGG AAAGGCCCAA GACTCTCTCC CCAGGGAAGA ATGGGGTCGT CAAAGACGTT TTTGCCTTTG GGGGTGCCGT GGAGAACCCC GAGTACTTGA CACCCCAGGG AGGAGCTGCC CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG ACAACCTCTA TTACTGGGAC CAGGACCCAC CAGAGCGGGG GGCTCCACCC AGCACCTTCA AAGGGACACC TACGGCAGAG AACCCAGAGT ACCTGGGTCT GGACGTGCCA GTGGGTAACC GAGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTG AATAACAGGG TAATCCGCTA GCCCCTCTCC CTCCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT ACACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACACTAGA GATCCACCGG TCGCCACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAAGCG GCCGCGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAATTCG AGCTCGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTGC
