NM_001110140.3;9530097L16Rik; D5Wsu150e; mKIAA4195;SERCA2; Serca2a; SERCA2B
100μg liquid plasmid(Instantly):$120
| name | pCMV-ATP2A2(mouse)-FLAG-EGFP-Puro | another name | apCMV-ATP2A2(mouse)-FLAG-EGFP-Puro |
| Insert Size (bp) | 3135bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCCA CCATGGAGAA CGCTCACACA AAGACCGTGG AGGAGGTGCT GGGCCACTTC GGGGTCAACG AGAGCACGGG GCTGAGCTTG GAGCAGGTCA AGAAGCTCAA GGAGAGATGG GGCTCCAACG AATTGCCGGC TGAAGAAGGA AAAACCTTGC TGGAACTTGT GATTGAGCAG TTTGAAGACT TACTAGTTAG AATTTTACTG CTGGCAGCAT GTATATCTTT CGTTTTGGCT TGGTTCGAGG AAGGGGAAGA AACGATTACA GCCTTTGTAG AGCCGTTTGT AATTCTGCTT ATCTTGGTAG CCAATGCAAT CGTGGGTGTG TGGCAGGAAA GAAATGCTGA AAATGCAATA GAAGCTCTTA AGGAATATGA GCCTGAAATG GGCAAAGTGT ATCGACAGGA CAGAAAGAGT GTGCAACGAA TTAAAGCTAA AGACATAGTT CCTGGTGATA TAGTGGAAAT TGCTGTTGGT GACAAAGTTC CTGCTGATAT TAGATTGACA TCCATCAAGT CTACAACTCT AAGAGTCGAC CAGTCAATTC TTACAGGTGA ATCTGTCTCC GTCATCAAGC ATACTGACCC TGTCCCTGAC CCCCGAGCTG TTAATCAAGA CAAAAAGAAC ATGCTCTTTT CTGGTACAAA CATTGCTGCT GGGAAAGCTA TGGGAGTGGT GGTGGCAACT GGAGTTAATA CTGAGATCGG CAAGATCCGG GATGAAATGG TGGCAACAGA ACAGGAGAGA ACACCCCTAC AGCAGAAGCT AGACGAGTTT GGGGAGCAGC TTTCCAAAGT TATCTCCCTC ATTTGCATTG CAGTCTGGAT CATCAACATT GGGCATTTCA ATGACCCAGT TCATGGTGGC TCCTGGATCA GGGGTGCCAT CTACTACTTT AAGATTGCCG TGGCCCTGGC TGTTGCCGCA ATCCCTGAGG GTCTGCCTGC TGTCATCACC ACCTGCTTAG CTCTTGGAAC TCGTAGGATG GCAAAGAAAA ATGCTATCGT TCGAAGTCTG CCTTCTGTGG AGACCCTTGG TTGTACTTCT GTTATCTGCT CAGATAAGAC AGGCACACTT ACCACAAACC AGATGTCCGT GTGCAGGATG TTCATTCTGG ACAAAGTAGA AGGTGACACT TGTTCCCTTA ATGAGTTCAG CATAACTGGA TCCACATATG CACCAATTGG AGAAGTGCAA AAGGATGATA AGCCAGTGAA GTGCCATCAG TATGACGGGC TTGTAGAGTT AGCCACCATC TGTGCTCTGT GTAATGACTC TGCTTTGGAT TATAATGAGG CAAAGGGTGT GTATGAGAAA GTTGGAGAAG CTACCGAGAC TGCTCTCACG TGCCTGGTGG AGAAGATGAA TGTATTTGAT ACTGAGCTGA AGGGGCTTTC TAAAATAGAG CGTGCAAACG CCTGCAACTC GGTCATAAAG CAGCTGATGA AGAAGGAGTT CACTCTGGAG TTTTCACGGG ATAGAAAATC AATGTCCGTC TATTGTACCC CAAACAAGCC AAGCCGGACA TCCATGAGCA AGATGTTTGT GAAGGGGGCT CCAGAAGGTG TCATCGATAG GTGCACCCAC ATCCGAGTTG GAAGTACCAA GGTCCCCATG ACTCCTGGTG TCAAACAGAA GATTATGTCT GTCATTCGGG AGTGGGGCAG TGGCAGCGAC ACGCTACGGT GCCTGGCTCT GGCCACTCAT GACAACCCAC TGAAGAGAGA GGAGATGCAC CTGGAAGACT CTGCTAACTT CATCAAATAC GAGACCAACC TGACTTTCGT CGGCTGTGTG GGCATGCTGG ATCCTCCCAG GATTGAAGTA GCCTCTTCTG TGAAGCTGTG CCGGCAAGCA GGCATCCGGG TCATCATGAT CACTGGAGAC AACAAAGGCA CCGCTGTGGC CATCTGTCGC CGCATTGGCA TCTTTGGGCA GGATGAGGAT GTGACATCAA AGGCTTTTAC AGGGCGAGAG TTTGATGAAT TAAGCCCTTC AGCCCAGAGA GATGCCTGCT TAAATGCCCG CTGTTTTGCT CGAGTTGAAC CTTCCCACAA GTCTAAGATT GTTGAGTTCC TTCAGTCCTT TGATGAGATC ACAGCTATGA CTGGTGATGG TGTGAATGAT GCTCCTGCTC TGAAGAAATC TGAAATCGGG ATTGCCATGG GCTCAGGGAC TGCAGTGGCT AAGACTGCTT CTGAGATGGT CCTGGCAGAT GACAACTTCT CCACCATTGT GGCTGCTGTT GAGGAGGGGC GAGCCATCTA CAACAACATG AAGCAGTTCA TCCGCTACCT CATCTCATCC AACGTGGGGG AAGTGGTCTG TATCTTCCTG ACGGCAGCCC TTGGGTTTCC TGAGGCTTTA ATTCCTGTCC AGTTACTCTG GGTCAATCTG GTGACAGATG GTCTGCCTGC CACTGCGCTG GGGTTCAATC CTCCAGACCT GGACATCATG AACAAACCCC CCCGGAACCC AAAAGAACCA CTGATCAGCG GGTGGCTCTT TTTCCGTTAC CTGGCTATTG GCTGTTATGT TGGCGCTGCC ACCGTGGGTG CTGCTGCATG GTGGTTCATC GCTGCTGACG GCGGTCCAAG AGTCTCCTTC TACCAGCTGA GTCATTTCCT ACAGTGTAAG GAGGACAACC CAGACTTCGA TGGAGTGGAT TGTGCAATCT TTGAGTCCCC ATATCCAATG ACAATGGCAC TTTCTGTTCT AGTAACCATA GAGATGTGTA ATGCCCTCAA CAGCTTGTCT GAAAACCAGT CTTTGCTGAG GATGCCCCCC TGGGAGAATA TCTGGCTCGT GGGCTCCATC TGCTTGTCCA TGTCACTTCA CTTCTTGATC CTCTACGTGG AACCTTTGCC GCTCATTTTC CAGATCACAC CGCTGAATCT GACCCAGTGG CTGATGGTGC TGAAAATCTC CTTGCCTGTG ATCCTCATGG ATGAGACGCT CAAGTTTGTG GCCCGAAACT ACCTGGAACA ACCCGGTAAA GAGTGTGTGC AGCCTGCCAC CAAATCTTCC TGCTCCCTGT CGGCATGCAC CGATGGCATT TCCTGGCCGT TTGTGCTGCT CATTATGCCC CTGGTGGTCT GGGTCTACAG CACAGACACT AACTTTAGCG ATATGTTCTG GTCTGATTAC AAGGATGACG ACGATAAGTG ACTCGAGCTC AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCGATCCGC CCCTCTCCCT CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTAC ACATGCTTTA CATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCTAG GAGACGACCT TCCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCG GGATCCACCG GATCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGATCA AGCTCCAATT CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG CTAGTGGATC CCCCGGGCTG CAGGAATTCG ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCG
