pU6-ZFP69B(human)-sgRNA3-Cas9-EGFP-Puro
$84.00
In stock
SKU
PL47638
100μg liquid plasmid(Instantly):$105
| name | pU6-ZFP69B(human)-sgRNA3-Cas9-EGFP-Puro | another name | apU6-ZFP69B(human)-sgRNA3-Cas9-EGFP-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAGAGCAGG CTGGTACCGG TCCGGAATTC CCGGGATATC GTCGACCCAC GCGTCCGCGG ACGCGTGGGC GGACGCGTGG GCGGACGCGT GGGAGCCCGC GGCGCCCGGC CACCGTCCCC TTGCCCACCG AGACACTCAG GAAGGCAGGA CCCTGGCCAC TGTACCTGCC TTAAACCAGG CCGTTGCTCC GCTGTCTCCT CTTACTCACA AGGACTACAG AATGCTTGTG GGTTCTGTTT TGTCCACTTT TGTAACTCCA GGACCTTCCG CTCTCTACTA ATTCCTCTTG GCCGGTCCCC CAACAGTGCA TCCTCTCCTA GGGACACTCC ACACGACCTG GGCCTGGTTT TGGGCCTGCA AGCTCCCTCC TGATCGCTCC TCTTCACTTC TCCCCATCTT CCTGCTCCCT ATTTATCGCC TGCCTTCTGT CTATCGTTCC CCTTGTCACT TCTTGGGGCT CCTCCTAAGT AGGGGCAGGT CGGTACCCCT CGCCCCAGGA TGTGAGACCC TTTAACCTGT AATGCTGTGG CTGGCCCTTG GCCCCTTCTG TGCCATGGAG AACCAGGTGC TGGTGATTCG GATCAAGATT CCAAATAGTG GCGCGGTGGA CTGGACCGTG CACTCCGGGC CGCAGTTACT CTTCAGGGAT GTTCTGGATG TGATAGGCCA GGTTCTGCCT GAAGCGACGA CGACAGCGTT TGAATATGAA GATGAAGATG GTGATAGGAT TACAGTAAGA AGCGATGAAG AGATGAAGGC AATGCTGTCC TACTATTATT CCACAGTAAT GGAACAGCAA GTAAATGGCC AGCTAATAGA GCCGCTGCAG ATATTTCCAA GAGCCTGCAA GCCTCCTGGG GAACGGAACA TACATGGCCT GAAGGTGAAT ACACGGGCTG GGCCATCTCA ACACACCAGC CCTGTGGTCT CAGATTCACT TCCAAGCAAT AGCTTGAAGA AGTCCTCAGC TGAACTGAGA AAGATACTGG CCAACGGCCA GATGAATGAA CAAGACATAC GGTATCGAGA CACCCTTGGT CATGGCAACG GAGGCACAGT CTACAAAGCA CATCATGTCC CAAGTGGGAA AATTTTAGCT GTAAAGGTTA TTCTGTTAGA CATTACACTG GAGCTTCAGA AGCAGATCAT GTCTGAGCTG GAAATCCTTT ATAAGTGTGA CTCATCGTAT ATCATAGGAT TTTACGGGGC ATTTTTCGTA GAAAACAGGA TTTCAATTTG TACAGAATTC ATGGATGGGG GGTCTTTGGA TGTATATAGG AAAATTCCAG AGCACGTCCT TGGAAGAATT GCAGTTGCAG TTGTTAAAGG CCTTACCTAT CTGTGGAGTT TAAAGATTTT ACACAGAGAT GTGAAGCCCT CCAACATGCT TGTAAACACA GGCGGACAGG TCAAGCTGTG TGACTTTGGC GTGAGCACAC AGCTGGTGAA TTCTATAGCC AAGACGTATG TTGGAACAAA TGCTTACATG GCACCAGAAA GAATTTCAGG AGAGCAGTAT GGGATCCATT CTGACGTGTG GAGCTTAGGA ATCTCTTTCA TGGAGCTTGC TCTTGGGAGG TTTCCATATC CTCAGCCTCT CCAGCTTCTG CAGTGCATTG TTGATGAGGA TTCGCCGGTC CTTCCTCTTG GAGAGTTCTC GGAGCCGTTT GTACATTTCA TCACTCAGTG CATGAGGAAG CAGCCCAAGG AGAGACCGGC GCCTGAGGAG CTGATGGGTC ACCCATTCAT CGTGCAGTTC AATGATGGAA ACTCCACTGT GGTGTCCATG TGGGTGTGCC GAGCTCTAGA GGAGAGGCGG AGCCAGCAGG GACCCCCGTG AGACCTCAGC AGGACACTGA CCACTGAGGA CCAGTCATCA TGACACAGCA AGCCGTGACC CCTCTGTCTG CTGTACTGGC CCAGCCTCCT GCTCGCACCT CCTGCCCTAC CTAGGAAGCC CTCCAGAGGG CCAGCCTCTG CTGAGCGGGG GAAGTGGGCA CTGACTATAG CAGGTGGAGA TGGTGCAGGG CGGGGTGATG AGGTTATAAA ACTATGGAAC TTCCTCTGGC CCCACCCCCT TATCTTTCCC TACTGTTCAT TGTAAAGGGT CAGGCCCTAT CAGCAGCACT GATGGGAATA AAAGTATTAT TGCTTTGGAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAGGGCG GCCGCTCTAG AGTATCCCTC GAGGGGCCCA AGCTTACGCG TACCCAGCTT TCTTGTACAA AGTGGTCCCT ATAAGGGAGT CGTATTATAA GCTAGGCACT GGGCCGTCGT TTTACAACCG CCGTGACTGG GGAAAACTGC TAAGCTTGGG GATCTTTGGG GAAGGAACCT TACTTTCTGG GGGGGGGACA
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