NM_001039581.2;1810036E22Rik; ABC-C; Abc3
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-ABCA3(mouse)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-ABCA3(mouse)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 5115bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CAGTGCTGCG GCAGCTCACC CTCCTACTCT GGAAGAACTA TACCCTGAAG AAACGGAAGG TTCTAGTGAC AGTCCTGGAG CTCTTCCTGC CCCTGCTGTT TTCTGGGATC CTGATTTGGC TTCGCTTGAA GATCCAGTCG GAGAATGTGC CCAATGCCAC TGTTTACCCG GACCAGTCCA TCCAGGAGCT GCCCCTGTTT TTCTCATTCC CTCCACCTGG GGGGACCTGG GAGCTCGCCT ATGTCCCTTC CCACAGCGAT GCTGCCAGGA CCATCACGGA GACGGTGAAA AGGGAATTCA TGATCAAGAT GAGAGTGCAC GGCTTCTCCT CTGAGAAGGA CTTCGAGGAC TACATTCGCT ATGACAACCA CTCCTCCAGC GTGCTGGCAG CTGTGGTATT TGAGCACAGC TTCAACCACA GCCAGGACCC CCTGCCGCTG GCGGTGAAAT ACCACCTGCG CTTCAGTTAC ACACGAAGAA ATTACATGTG GACCCAAACA GGAAATATTT TCCTAAAAGA GACTGAAGGC TGGCATACCA CTTCACTTTT TCCACTTTTT CCAAGCCCCG GACCGCGGGA ACCTTCATCA CCTGATGGCG GTGAACCTGG ATACATTCGG GAAGGCTTCC TGGCCATGCA ACATGCTGTA GACAAAGCCA TCATGCGTTA CCATGCCAAC ACCTCTGCAC AGCAGCTGTT CCAGAAGCTC ATGGTGATCA CCAAAAGGTT CCCGTTCCCA CCATACATCT CAGATCCATT CCTCATTGCC ATCCAGTACC AGCTTCCTCT GCTGCTCATG CTGAGTTTCA CCTACACCTC CCTCACCATC ATCCGGGCTG TGGTGCAGGA GAAGGAGAAA AAACTAAAGG AGTACATGCG CATGATGGGG CTTAACAGCT GGCTACACTG GAGCGCTTGG TTCCTCATGT TCTTCCTATT CTTCCTCATC GTGGTCTCCT TCATGACCCT CCTGTTCTGT GTCAAAGTGA AGAAGGACAT AGCAGTCCTT TCCAACAGCG ACCCCTCCCT GGTCCTGGCG TTCCTGCTGT GTTTTGCAAT CTCCTCCATT TCCTTCAGCT TCATGGTCAG CACCTTCTTC AGTAAAGCCA ACATAGCAGC AGCCGTGGGT GGCTTCCTGT ACTTCTTCAC CTATACCCCC TACTTTTTCG TGGCTCCTCG GTACAACTGG ATGACACTGA GTCAAAAGCT CTTGTCCTGT CTCCTATCTA ATGTTGCCAT GGCAATGGGA GCCCAGCTCA TAGGAAAATT CGAAGCCAAA GGCACTGGCA TCCAGTGGCG GGACCTCCTC AACCCTGTCA ACGTGGACGA CAATTTCTGC TTTGGACAAG TGCTGGGGAT GCTGCTGCTG GACTCAGCAC TCTATGGCCT GGTGACCTGG TACGTGGAGG CTGTCTTCCC AGGCCAGTTT GGCGTACCCC AGCCCTGGCA CTTCTTCCTT ATGCCCTCCT ACTGGTGTGG GAACCCAAGA ACTGTCGTAG GGAAGGAAGA GGAAGGCAGC GACCCAGAGA AAGCTCTCAG GAACGAGTAC TTTGAAGCCG AGCCAGAGGA CTTGGTAGCT GGAATCAAGA TCAAGCATCT CTCCAAGGTG TTCCAAGTGG GCAATAAGGA CAAGATGGGC ATCAGAGACC TGACCCTGAA CCTCTACGAA GGACAGATAA CAGTCCTCCT GGGCCACAAT GGTGCTGGGA AGACCACGAC CATGTCTCTG CTGACAGGCC TCTTCCCCCC TACCAGTGGA CATGCTTATA TTCATGGGTA CGAAATTTCC CAAGACATGG CTCAGATCCG CAAGAGTTTG GGCCTGTGTC CCCAGCATGA TGTACTGTTT GACAACCTGA CAGTTGCCGA ACATCTTTAC TTCTATGCAC AGCTGAAAGG CCTCTCTCTC CAGAAGTGTC CTGAGGAAGT CAAGCAGATG CTACACATCC TCAGCCTGGA GGACAAGCGT GACTTACGGT CCAAGTTCCT GAGTGGGGGC ATGAAGCGCA AGCTCTCCAT TGGCATCGCC CTCATAGCGG GCTCTAAGGT GCTGATGTTA GATGAGCCCA CCTCAGGCAT GGATGCAGTG TCCAGAAGGG CCATCTGGGA CCTTCTCCAG CAGCAGAAGA GTGACCGCAC CGTCCTGCTG ACCACTCACT TCATGGACGA AGCTGACCTG CTGGGAGACC GCATCGCCAT CTTGGCCAAG GGGGAGCTCC AGTGCTGCGG GTCTTCACTA TTTCTCAAGC AGAAATACGG AGCTGGCTAC CACATGACGC TGGTAAAAGA ACCGCACTGC AATCCTGAAG GAATCTCTCA GCTGGTGCAC CACCATGTCC CCAATGCCAT GCTGGAGAGC CATGCTGGGG CTGAGCTGTC CTTTATTCTT CCCAAGGAGA GCACGCACAG GTTTGAAAGT CTGTTTGCTA AACTGGAAAA GAAGCAGAAG GAGTTGGGCA TCGCAAGCTT CGGGGCCTCG GTCACCACCA TGGAGGAAGT CTTCCTTCGG GTTGGTAAGC TGGTGGACAC CAGCATGGAC ATTCAAGCCA TTCAGCTTCC TGCTCTGCAG TACCAGCATG AGAGGCGGGC AAGCGACTGG GCTTTGGACA GTAACCTGTG TGGGGTGATG GACCCAACGA ATGGCATTGG AGCCCTCATA GAGGAAGAGG AAGTTTTGGT CAAGCTCAAC ACAGGGCTTG CCCTCCACTG CCAGCAGTTC TGGGCCATGT TCCTGAAGAA GGCTGCATAC AGCTGGCGGG AATGGAAGAT GGTGGCAGCT CAGGTCCTGG TCCCCCTGAC CTGCCTCACC CTGGCCCTCC TGGCTATCCA CTACACCTCA GAGATCTTTG ATGACCCTCT CCTGAAGCTA AGCCTGAACG AGTATGGTAG AACGGTTGTG CCCTTCTCGG TCCCAGGCAC ATCCAGGCTG GCTCAGCAGC TGTCTGAGAA TCTAAGGGAC ATGCTGCAGG CTGAGAGGCA GGAGCCCCGG GAAGTGCTAG GTGACCTAGA GGAGTTCCTG GTTTTCAGGG CCTCAGTGGA AGGAGGCGGC TTTAATGAGC GGTGCCTAGT AGCAACATCC TTCAAAGACA GAGGAGAGCT GACCGTGGTC ACTGCCCTGT TCAACAACCA GGCATACCAC TCCCCAGCCA CTGCCCTGGC CATCGTGGAC AACCTTCTGT TCAAGCTATT GTGTGGCCCT CAGGCCTCCA TTGAGATCTC CAACTACCCC CAGCCCCGGA ACACCCTGCA GGTCGCCAAG GACCACTTCA ATGAGGGCCG GAAGGGATTT GACATTGCCC TCAATTTGCT CATCGCCATG GCATTTCTGG CCAGCACCTT TTCCATCCTG GCTGTCAGTG AGAGGGCCGT CCAGGCCAAG CACGTTCAGT TTGTGAGCGG TGTCCATGTG GCTACTTTCT GGTTCTCCGC TCTGTTGTGG GACCTCATCT CCTTCCTCGT TCCCAGTCTA CTACTTCTGG TGGTGTTCCA AGCCTTCAAT GTGCACGCCT TCACGAGGGA TGGTCACATG GCTGACCTGC TGCTGCTGCT CATGCTCTAC GGCTGGGCCA TCATCCCCCT CATGTACCTC ATGAGCTTCT TCTTCTCGGC TGCGTCCACA GCTTATACCA GGCTGACCAT ATTCAACATC CTGTCGGGCA TCGCTACCTT CATCATGGTC ACTATCATGC GTATCCCAGC TGTGAAGTTA GAAGAACTTT CCAGAACCTT AGATCATGTG TTCTTGGTGC TGCCAAACCA CTGCCTGGGG ATGGCGGTCA GCAACTTCTA TGAGAACTAT GAGACTCGGC GATACTGCAC TTCCTCAGAG CTTGCTGCCC ACTACTGCAA GAAGTACAAC ATCCAGTACC AGGAGAGCTT CTATGCCTGG AGCACCCCAG GCGTGGGCAA GTTTGTGACT TCCATGGCTG CCTCAGGGGG CATCTACCTC ACCCTGCTGT TCCTCATTGA GACCAACCTG CTGTGGCGAC TGAGAACCTT CATCTGTGCC TTCCGGAGGA GGTGGACTCT GGCAGAACTG CAGAACCGGA CATCAGTGCT GCCCGAGGAC CAGGATGTAG CTGAGGAGAG GAGCCGAATC CTGGTCCCTA GCTTGGACTC CATGCTCGAC ACACCACTGA TCATCAACGA GCTCTCCAAG GTGTATGACC AGCGAGCACC GCTCCTTGCC GTGGACAGGA TCTCCCTTGC GGTCCAGAAA GGGGAGTGCT TCGGCCTGTT GGGTTTCAAT GGAGCTGGAA AAACCACAAC ATTCAAAATG CTGACTGGGG AGGAGACCAT CACCTCAGGG GATGCCTTTG TTGGTGGTTA CAGCATCAGT TCTGACATCG GGAAGGTGCG GCAGCGGATG GGCTACTGCC CCCAGTTTGA TGCACTGCTT GATCACATGA CTGGCAGGGA GATGCTGGTT ATGTATGCAC GGCTCCGAGG CATCCCAGAG CGGCTCATCA ATGCCTGTGT GGAGAATACT CTGCGGGGTC TGCTGCTGGA ACCGCACGCC AACAAACTAG TCAAGACTTA CAGTGGTGGT AACAAACGCA AGCTAAGCAC TGGCATTGCC CTCATTGGAG AGCCTGCAGT CATCTTCCTG GATGAGCCAT CGACCGGCAT GGACCCAGTG GCCCGGCGTC TACTCTGGGA TACTGTAGCC CGGGCCCGAG AGTCTGGCAA AGCCATTGTC ATCACCTCCC ACAGTATGGA GGAGTGTGAA GCCTTATGTA CCAGGCTGGC CATCATGGTA CAGGGCCAGT TCAAGTGCCT GGGCAGCCCA CAGCACCTCA AGAGCAAGTT TGGCAGCGGC TACTCCCTGC AGGCCAAGGT CCGGAGCGAG GGGAAGCAAG ACGCGCTAGA GGAGTTCAAG GCCTTTGTGG ACCTGACCTT CCCAGGCAGC ATCCTAGAAG ATGAGCACCA AGACATGGTC CATTATCATC TGCCTGGCTG TGACCTCAGC TGGGCCAAGG TATTTGGTAT CCTGGAGAAA GCCAAGGAGA AGTATGGAGT GGATGATTAC TCTGTGAGCC AGATCTCCCT GGAGCAGGTC TTTCTGAGCT TTGCCCACCT ACAACCCCCC ACCACTGAGG ATGGCCGGCT CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGGGATCCC TGCGATATCC AGCGCGGCCG CGAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC CATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCA AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG GTGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGTGC TGGTGGGCAG CTTCGCCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTTCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGC TGCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCCATCG CCTTCGCCAG ATCCCGCGCT CAGTCGTCCA ATTCTGCCGT GGACGGCACC GCCGGACCCG GCTCCACCGG ATCTCGCGAG GGCAGAGGAA GTCTTCTAAC ATGCGGTGAC GTGGAGGAGA ATCCCGGCCC TATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT CCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGCCG CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG
