pLV3-CMV-ITGA7(human)-TagRFP-Puro
$99.00
In stock
SKU
PL47165
NM_001144996.2
100μg liquid plasmid(Instantly):$120
| name | pLV3-CMV-ITGA7(human)-TagRFP-Puro | another name | apLV3-CMV-ITGA7(human)-TagRFP-Puro |
| Insert Size (bp) | 3426bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGCCGGG GCTCGGAGCC GCGACCCTTG GGGGGCCTCC GGGATTTGCT ACCTTTTTGG CTCCCTGCTC GTCGAACTGC TCTTCTCACG GGCTGTCGCC TTCAATCTGG ACGTGATGGG TGCCTTGCGC AAGGAGGGCG AGCCAGGCAG CCTCTTCGGC TTCTCTGTGG CCCTGCACCG GCAGTTGCAG CCCCGACCCC AGAGCTGGCT GCTGGTGGGT GCTCCCCAGG CCCTGGCTCT TCCTGGGCAG CAGGCGAATC GCACTGGAGG CCTCTTCGCT TGCCCGTTGA GCCTGGAGGA GACTGACTGC TACAGAGTGG ACATCGACCA GGGAGCTGAT ATGCAAAAGG AAAGCAAGGA GAACCAGTGG TTGGGAGTCA GTGTTCGGAG CCAGGGGCCT GGGGGCAAGA TTGTTACCTG TGCACACCGA TATGAGGCAA GGCAGCGAGT GGACCAGATC CTGGAGACGC GGGATATGAT TGGTCGCTGC TTTGTGCTCA GCCAGGACCT GGCCATCCGG GATGAGTTGG ATGGTGGGGA ATGGAAGTTC TGTGAGGGAC GCCCCCAAGG CCATGAACAA TTTGGGTTCT GCCAGCAGGG CACAGCTGCC GCCTTCTCCC CTGATAGCCA CTACCTCCTC TTTGGGGCCC CAGGAACCTA TAATTGGAAG GGCACGGCCA GGGTGGAGCT CTGTGCACAG GGCTCAGCGG ACCTGGCACA CCTGGACGAC GGTCCCTACG AGGCGGGGGG AGAGAAGGAG CAGGACCCCC GCCTCATCCC GGTCCCTGCC AACAGCTACT TTGGCTTCTC TATTGACTCG GGGAAAGGTC TGGTGCGTGC AGAAGAGCTG AGCTTTGTGG CTGGAGCCCC CCGCGCCAAC CACAAGGGTG CTGTGGTCAT CCTGCGCAAG GACAGCGCCA GTCGCCTGGT GCCCGAGGTT ATGCTGTCTG GGGAGCGCCT GACCTCCGGC TTTGGCTACT CACTGGCTGT GGCTGACCTC AACAGTGATG GCTGGCCAGA CCTGATAGTG GGTGCCCCCT ACTTCTTTGA GCGCCAAGAA GAGCTGGGGG GTGCTGTGTA TGTGTACTTG AACCAGGGGG GTCACTGGGC TGGGATCTCC CCTCTCCGGC TCTGCGGCTC CCCTGACTCC ATGTTCGGGA TCAGCCTGGC TGTCCTGGGG GACCTCAACC AAGATGGCTT TCCAGATATT GCAGTGGGTG CCCCCTTTGA TGGTGATGGG AAAGTCTTCA TCTACCATGG GAGCAGCCTG GGGGTTGTCG CCAAACCTTC ACAGGTGCTG GAGGGCGAGG CTGTGGGCAT CAAGAGCTTC GGCTACTCCC TGTCAGGCAG CTTGGATATG GATGGGAACC AATACCCTGA CCTGCTGGTG GGCTCCCTGG CTGACACCGC AGTGCTCTTC AGGGCCAGAC CCATCCTCCA TGTCTCCCAT GAGGTCTCTA TTGCTCCACG AAGCATCGAC CTGGAGCAGC CCAACTGTGC TGGCGGCCAC TCGGTCTGTG TGGACCTAAG GGTCTGTTTC AGCTACATTG CAGTCCCCAG CAGCTATAGC CCTACTGTGG CCCTGGACTA TGTGTTAGAT GCGGACACAG ACCGGAGGCT CCGGGGCCAG GTTCCCCGTG TGACGTTCCT GAGCCGTAAC CTGGAAGAAC CCAAGCACCA GGCCTCGGGC ACCGTGTGGC TGAAGCACCA GCATGACCGA GTCTGTGGAG ACGCCATGTT CCAGCTCCAG GAAAATGTCA AAGACAAGCT TCGGGCCATT GTAGTGACCT TGTCCTACAG TCTCCAGACC CCTCGGCTCC GGCGACAGGC TCCTGGCCAG GGGCTGCCTC CAGTGGCCCC CATCCTCAAT GCCCACCAGC CCAGCACCCA GCGGGCAGAG ATCCACTTCC TGAAGCAAGG CTGTGGTGAA GACAAGATCT GCCAGAGCAA TCTGCAGCTG GTCCGCGCCC GCTTCTGTAC CCGGGTCAGC GACACGGAAT TCCAACCTCT GCCCATGGAT GTGGATGGAA CAACAGCCCT GTTTGCACTG AGTGGGCAGC CAGTCATTGG CCTGGAGCTG ATGGTCACCA ACCTGCCATC GGACCCAGCC CAGCCCCAGG CTGATGGGGA TGATGCCCAT GAAGCCCAGC TCCTGGTCAT GCTTCCTGAC TCACTGCACT ACTCAGGGGT CCGGGCCCTG GACCCTGCGG AGAAGCCACT CTGCCTGTCC AATGAGAATG CCTCCCATGT TGAGTGTGAG CTGGGGAACC CCATGAAGAG AGGTGCCCAG GTCACCTTCT ACCTCATCCT TAGCACCTCC GGGATCAGCA TTGAGACCAC GGAACTGGAG GTAGAGCTGC TGTTGGCCAC GATCAGTGAG CAGGAGCTGC ATCCAGTCTC TGCACGAGCC CGTGTCTTCA TTGAGCTGCC ACTGTCCATT GCAGGAATGG CCATTCCCCA GCAACTCTTC TTCTCTGGTG TGGTGAGGGG CGAGAGAGCC ATGCAGTCTG AGCGGGATGT GGGCAGCAAG GTCAAGTATG AGGTCACGGT TTCCAACCAA GGCCAGTCGC TCAGAACCCT GGGCTCTGCC TTCCTCAACA TCATGTGGCC TCATGAGATT GCCAATGGGA AGTGGTTGCT GTACCCAATG CAGGTTGAGC TGGAGGGCGG GCAGGGGCCT GGGCAGAAAG GGCTTTGCTC TCCCAGGCCC AACATCCTCC ACCTGGATGT GGACAGTAGG GATAGGAGGC GGCGGGAGCT GGAGCCACCT GAGCAGCAGG AGCCTGGTGA GCGGCAGGAG CCCAGCATGT CCTGGTGGCC AGTGTCCTCT GCTGAGAAGA AGAAAAACAT CACCCTGGAC TGCGCCCGGG GCACGGCCAA CTGTGTGGTG TTCAGCTGCC CACTCTACAG CTTTGACCGC GCGGCTGTGC TGCATGTCTG GGGCCGTCTC TGGAACAGCA CCTTTCTGGA GGAGTACTCA GCTGTGAAGT CCCTGGAAGT GATTGTCCGG GCCAACATCA CAGTGAAGTC CTCCATAAAG AACTTGATGC TCCGAGATGC CTCCACAGTG ATCCCAGTGA TGGTATACTT GGACCCCATG GCTGTGGTGG CAGAAGGAGT GCCCTGGTGG GTCATCCTCC TGGCTGTACT GGCTGGGCTG CTGGTGCTAG CACTGCTGGT GCTGCTCCTG TGGAAGATGG GATTCTTCAA ACGGGCGAAG CACCCCGAGG CCACCGTGCC CCAGTACCAT GCGGTGAAGA TTCCTCGGGA AGACCGACAG CAGTTCAAGG AGGAGAAGAC GGGCACCATC CTGAGGAACA ACTGGGGCAG CCCCCGGCGG GAGGGCCCGG ATGCACACCC CATCCTGGCT GCTGACGGGC ATCCCGAGCT GGGCCCCGAT GGGCATCCAG GGCCAGGCAC CGCCGGCGGA GGTGGTTCAG GTGGCGGAGG TTCTGGATCC ATGGTGTCTA AGGGCGAAGA GCTGATTAAG GAGAACATGC ACATGAAGCT GTACATGGAG GGCACCGTGA ACAACCACCA CTTCAAGTGC ACATCCGAGG GCGAAGGCAA GCCCTACGAG GGCACCCAGA CCATGAGAAT CAAGGTGGTC GAGGGCGGCC CTCTCCCCTT CGCCTTCGAC ATCCTGGCTA CCAGCTTCAT GTACGGCAGC AGAACCTTCA TCAACCACAC CCAGGGCATC CCCGACTTCT TTAAGCAGTC CTTCCCTGAG GGCTTCACAT GGGAGAGAGT CACCACATAC GAAGACGGGG GCGTGCTGAC CGCTACCCAG GACACCAGCC TCCAGGACGG CTGCCTCATC TACAACGTCA AGATCAGAGG GGTGAACTTC CCATCCAACG GCCCTGTGAT GCAGAAGAAA ACACTCGGCT GGGAGGCCAA CACCGAGATG CTGTACCCCG CTGACGGCGG CCTGGAAGGC AGAAGCGACA TGGCCCTGAA GCTCGTGGGC GGGGGCCACC TGATCTGCAA CTTCAAGACC ACATACAGAT CCAAGAAACC CGCTAAGAAC CTCAAGATGC CCGGCGTCTA CTATGTGGAC CACAGACTGG AAAGAATCAA GGAGGCCGAC AAAGAGACCT ACGTCGAGCA GCACGAGGTG GCTGTGGCCA GATACTGCGA CCTCCCTAGC AAACTGGGGC ACAAACTTAA TTGAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG
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