pGEM-ITGA7(human)
$99.00
In stock
SKU
PL47010
NM_001144996.2
100μg liquid plasmid(Instantly):$120
| name | pGEM-ITGA7(human) | another name | apGEM-ITGA7(human) |
| Insert Size (bp) | 3426bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGCGAATTG GGCCCGACGT CGCATGCTCC CGGCCGCCAT GGCCGCGGGA TTATGGCCGG GGCTCGGAGC CGCGACCCTT GGGGGGCCTC CGGGATTTGC TACCTTTTTG GCTCCCTGCT CGTCGAACTG CTCTTCTCAC GGGCTGTCGC CTTCAATCTG GACGTGATGG GTGCCTTGCG CAAGGAGGGC GAGCCAGGCA GCCTCTTCGG CTTCTCTGTG GCCCTGCACC GGCAGTTGCA GCCCCGACCC CAGAGCTGGC TGCTGGTGGG TGCTCCCCAG GCCCTGGCTC TTCCTGGGCA GCAGGCGAAT CGCACTGGAG GCCTCTTCGC TTGCCCGTTG AGCCTGGAGG AGACTGACTG CTACAGAGTG GACATCGACC AGGGAGCTGA TATGCAAAAG GAAAGCAAGG AGAACCAGTG GTTGGGAGTC AGTGTTCGGA GCCAGGGGCC TGGGGGCAAG ATTGTTACCT GTGCACACCG ATATGAGGCA AGGCAGCGAG TGGACCAGAT CCTGGAGACG CGGGATATGA TTGGTCGCTG CTTTGTGCTC AGCCAGGACC TGGCCATCCG GGATGAGTTG GATGGTGGGG AATGGAAGTT CTGTGAGGGA CGCCCCCAAG GCCATGAACA ATTTGGGTTC TGCCAGCAGG GCACAGCTGC CGCCTTCTCC CCTGATAGCC ACTACCTCCT CTTTGGGGCC CCAGGAACCT ATAATTGGAA GGGCACGGCC AGGGTGGAGC TCTGTGCACA GGGCTCAGCG GACCTGGCAC ACCTGGACGA CGGTCCCTAC GAGGCGGGGG GAGAGAAGGA GCAGGACCCC CGCCTCATCC CGGTCCCTGC CAACAGCTAC TTTGGCTTCT CTATTGACTC GGGGAAAGGT CTGGTGCGTG CAGAAGAGCT GAGCTTTGTG GCTGGAGCCC CCCGCGCCAA CCACAAGGGT GCTGTGGTCA TCCTGCGCAA GGACAGCGCC AGTCGCCTGG TGCCCGAGGT TATGCTGTCT GGGGAGCGCC TGACCTCCGG CTTTGGCTAC TCACTGGCTG TGGCTGACCT CAACAGTGAT GGCTGGCCAG ACCTGATAGT GGGTGCCCCC TACTTCTTTG AGCGCCAAGA AGAGCTGGGG GGTGCTGTGT ATGTGTACTT GAACCAGGGG GGTCACTGGG CTGGGATCTC CCCTCTCCGG CTCTGCGGCT CCCCTGACTC CATGTTCGGG ATCAGCCTGG CTGTCCTGGG GGACCTCAAC CAAGATGGCT TTCCAGATAT TGCAGTGGGT GCCCCCTTTG ATGGTGATGG GAAAGTCTTC ATCTACCATG GGAGCAGCCT GGGGGTTGTC GCCAAACCTT CACAGGTGCT GGAGGGCGAG GCTGTGGGCA TCAAGAGCTT CGGCTACTCC CTGTCAGGCA GCTTGGATAT GGATGGGAAC CAATACCCTG ACCTGCTGGT GGGCTCCCTG GCTGACACCG CAGTGCTCTT CAGGGCCAGA CCCATCCTCC ATGTCTCCCA TGAGGTCTCT ATTGCTCCAC GAAGCATCGA CCTGGAGCAG CCCAACTGTG CTGGCGGCCA CTCGGTCTGT GTGGACCTAA GGGTCTGTTT CAGCTACATT GCAGTCCCCA GCAGCTATAG CCCTACTGTG GCCCTGGACT ATGTGTTAGA TGCGGACACA GACCGGAGGC TCCGGGGCCA GGTTCCCCGT GTGACGTTCC TGAGCCGTAA CCTGGAAGAA CCCAAGCACC AGGCCTCGGG CACCGTGTGG CTGAAGCACC AGCATGACCG AGTCTGTGGA GACGCCATGT TCCAGCTCCA GGAAAATGTC AAAGACAAGC TTCGGGCCAT TGTAGTGACC TTGTCCTACA GTCTCCAGAC CCCTCGGCTC CGGCGACAGG CTCCTGGCCA GGGGCTGCCT CCAGTGGCCC CCATCCTCAA TGCCCACCAG CCCAGCACCC AGCGGGCAGA GATCCACTTC CTGAAGCAAG GCTGTGGTGA AGACAAGATC TGCCAGAGCA ATCTGCAGCT GGTCCGCGCC CGCTTCTGTA CCCGGGTCAG CGACACGGAA TTCCAACCTC TGCCCATGGA TGTGGATGGA ACAACAGCCC TGTTTGCACT GAGTGGGCAG CCAGTCATTG GCCTGGAGCT GATGGTCACC AACCTGCCAT CGGACCCAGC CCAGCCCCAG GCTGATGGGG ATGATGCCCA TGAAGCCCAG CTCCTGGTCA TGCTTCCTGA CTCACTGCAC TACTCAGGGG TCCGGGCCCT GGACCCTGCG GAGAAGCCAC TCTGCCTGTC CAATGAGAAT GCCTCCCATG TTGAGTGTGA GCTGGGGAAC CCCATGAAGA GAGGTGCCCA GGTCACCTTC TACCTCATCC TTAGCACCTC CGGGATCAGC ATTGAGACCA CGGAACTGGA GGTAGAGCTG CTGTTGGCCA CGATCAGTGA GCAGGAGCTG CATCCAGTCT CTGCACGAGC CCGTGTCTTC ATTGAGCTGC CACTGTCCAT TGCAGGAATG GCCATTCCCC AGCAACTCTT CTTCTCTGGT GTGGTGAGGG GCGAGAGAGC CATGCAGTCT GAGCGGGATG TGGGCAGCAA GGTCAAGTAT GAGGTCACGG TTTCCAACCA AGGCCAGTCG CTCAGAACCC TGGGCTCTGC CTTCCTCAAC ATCATGTGGC CTCATGAGAT TGCCAATGGG AAGTGGTTGC TGTACCCAAT GCAGGTTGAG CTGGAGGGCG GGCAGGGGCC TGGGCAGAAA GGGCTTTGCT CTCCCAGGCC CAACATCCTC CACCTGGATG TGGACAGTAG GGATAGGAGG CGGCGGGAGC TGGAGCCACC TGAGCAGCAG GAGCCTGGTG AGCGGCAGGA GCCCAGCATG TCCTGGTGGC CAGTGTCCTC TGCTGAGAAG AAGAAAAACA TCACCCTGGA CTGCGCCCGG GGCACGGCCA ACTGTGTGGT GTTCAGCTGC CCACTCTACA GCTTTGACCG CGCGGCTGTG CTGCATGTCT GGGGCCGTCT CTGGAACAGC ACCTTTCTGG AGGAGTACTC AGCTGTGAAG TCCCTGGAAG TGATTGTCCG GGCCAACATC ACAGTGAAGT CCTCCATAAA GAACTTGATG CTCCGAGATG CCTCCACAGT GATCCCAGTG ATGGTATACT TGGACCCCAT GGCTGTGGTG GCAGAAGGAG TGCCCTGGTG GGTCATCCTC CTGGCTGTAC TGGCTGGGCT GCTGGTGCTA GCACTGCTGG TGCTGCTCCT GTGGAAGATG GGATTCTTCA AACGGGCGAA GCACCCCGAG GCCACCGTGC CCCAGTACCA TGCGGTGAAG ATTCCTCGGG AAGACCGACA GCAGTTCAAG GAGGAGAAGA CGGGCACCAT CCTGAGGAAC AACTGGGGCA GCCCCCGGCG GGAGGGCCCG GATGCACACC CCATCCTGGC TGCTGACGGG CATCCCGAGC TGGGCCCCGA TGGGCATCCA GGGCCAGGCA CCGCCTAGAA TCACTAGTGC GGCCGCCTGC AGGTCGACCA TATGGGAGAG CTCCCAACGC GTTGGATGCA TAGCTTGAGT ATTCTATAGT GTCACCTAAA TAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATA
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