NM_198834.3;ACAC; Acac1; ACACAD; ACACalpha; ACC; ACC1;ACCA; ACCalpha; hACC1
100μg liquid plasmid(Instantly):$178
| name | pCMV-EGFP-ACACA(human)-GST-Neo | another name | apCMV-EGFP-ACACA(human)-GST-Neo |
| Insert Size (bp) | 7152bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGGC GGCGGAGGGA GTGGAGGAGG GGGAAGCAAG CTTATGTGGT GGTCTACTCT GATGTCAATC TTGAGGGCTA GGTCTTTCTG GAAGTGGATA TCTACTCAGA CAGTAAGAAT TATAAGAGCT GTAAGAGCTC ATTTTGGAGG AATAATGGAT GAACCATCTC CCTTGGCCCA ACCTCTGGAG CTGAACCAGC ACTCTCGATT CATAATAGGT TCTGTGTCTG AAGATAACTC AGAGGATGAG ATCAGCAACC TGGTGAAGTT GGACCTACTG GAGGAGAAGG AGGGCTCCTT GTCACCTGCT TCTGTTGGCT CAGATACACT CTCTGATTTG GGGATCTCTA GCCTACAGGA TGGCTTGGCC TTGCACATAA GGTCCAGCAT GTCTGGCTTG CACCTAGTAA AGCAGGGCCG AGACAGAAAG AAAATAGATT CTCAACGAGA TTTCACTGTG GCTTCTCCAG CAGAATTTGT TACTCGCTTT GGGGGAAATA AAGTGATTGA GAAGGTTCTT ATTGCTAACA ATGGCATTGC AGCAGTGAAA TGCATGCGGT CTATCCGTAG GTGGTCTTAT GAAATGTTTC GAAATGAACG TGCAATTAGA TTCGTTGTCA TGGTCACACC TGAAGACCTT AAAGCCAATG CAGAATACAT TAAGATGGCA GATCACTATG TGCCAGTGCC TGGAGGACCA AACAACAACA ACTATGCAAA TGTGGAATTA ATTCTTGATA TTGCTAAAAG GATCCCAGTA CAAGCAGTGT GGGCTGGCTG GGGTCATGCT TCTGAGAATC CCAAACTACC GGAACTTCTC TTGAAAAATG GCATTGCCTT CATGGGTCCT CCAAGCCAGG CCATGTGGGC TTTAGGGGAT AAGATTGCAT CTTCCATAGT GGCTCAAACT GCAGGTATCC CAACTCTTCC CTGGAGCGGC AGTGGTCTTC GTGTGGACTG GCAGGAAAAT GATTTTTCAA AACGTATCTT AAATGTTCCC CAGGAGCTAT ATGAAAAAGG TTATGTGAAA GATGTGGATG ATGGGCTACA GGCAGCTGAG GAAGTTGGAT ATCCAGTAAT GATCAAGGCC TCAGAGGGAG GAGGAGGGAA GGGAATTAGA AAAGTCAACA ATGCAGATGA CTTCCCTAAT CTCTTCAGAC AGGTTCAAGC TGAAGTTCCT GGATCTCCCA TATTTGTGAT GAGACTAGCC AAACAATCTC GTCATCTGGA GGTGCAGATC TTAGCGGACC AATATGGCAA TGCTATCTCT TTGTTTGGTC GTGATTGCTC TGTACAACGC AGGCATCAGA AGATTATTGA AGAAGCACCT GCTACTATTG CTACTCCAGC AGTATTTGAA CACATGGAAC AGTGTGCGGT GAAACTTGCC AAAATGGTGG GTTATGTGAG TGCTGGGACT GTGGAATACC TGTACAGCCA GGATGGCAGC TTCTACTTTC TGGAATTGAA TCCTCGGCTG CAGGTAGAGC ACCCTTGTAC AGAGATGGTG GCTGATGTCA ATCTCCCTGC AGCACAGCTC CAGATTGCCA TGGGGATTCC TCTATATAGA ATCAAGGATA TCCGTATGAT GTATGGGGTA TCTCCCTGGG GTGATTCTCC CATTGATTTT GAAGATTCTG CACACGTTCC TTGTCCAAGG GGCCATGTTA TTGCTGCTCG GATCACTAGT GAAAATCCAG ATGAGGGTTT TAAGCCCAGC TCAGGAACAG TTCAGGAGCT AAATTTCCGC AGCAATAAGA ATGTTTGGGG ATATTTCAGT GTTGCTGCTG CAGGGGGACT TCATGAATTT GCTGATTCTC AGTTTGGTCA CTGCTTTTCT TGGGGAGAAA ACAGAGAAGA GGCAATTTCA AACATGGTGG TGGCTTTGAA GGAGCTGTCT ATTCGGGGTG ACTTTCGAAC TACAGTTGAA TACCTGATCA AATTGTTAGA GACTGAAAGC TTTCAGATGA ACAGAATTGA TACTGGCTGG CTGGACAGAC TGATAGCAGA AAAAGTACAG GCTGAGCGAC CTGACACCAT GTTGGGGGTT GTGTGTGGTG CCCTCCACGT GGCAGATGTG AGCCTGCGGA ATAGCGTCTC TAACTTCCTT CACTCCTTAG AAAGGGGTCA AGTCCTTCCT GCTCATACAC TTCTGAATAC AGTAGATGTT GAACTTATCT ATGAGGGAGT CAAGTATGTA CTTAAGGTGA CTCGACAGTC CCCCAACTCC TATGTGGTGA TCATGAATGG CTCATGTGTA GAAGTAGATG TACATCGGCT GAGTGACGGT GGACTGCTCT TGTCCTATGA TGGCAGCAGT TATACTACGT ATATGAAAGA GGAAGTGGAT AGATATCGCA TCACAATTGG CAATAAAACC TGTGTGTTTG AGAAGGAAAA TGACCCATCG GTGATGCGCT CACCTTCTGC TGGGAAGTTA ATCCAGTACA TTGTAGAAGA TGGAGGTCAT GTGTTTGCCG GCCAGTGCTA TGCTGAGATT GAGGTAATGA AGATGGTAAT GACCTTAACA GCTGTGGAGT CTGGCTGTAT CCATTACGTC AAGCGACCTG GAGCAGCTCT TGACCCTGGC TGTGTACTAG CCAAAATGCA ACTGGACAAC CCCAGCAAGG TTCAGCAGGC TGAACTTCAC ACAGGTAGTC TGCCACGGAT CCAGAGCACG GCACTCAGAG GCGAGAAACT CCATCGAGTG TTCCATTATG TCCTGGATAA TCTGGTCAAT GTAATGAATG GATACTGCCT TCCAGATCCT TTCTTTAGCA GCAAGGTAAA AGACTGGGTA GAGCGATTGA TGAAAACCCT CAGAGATCCC TCCCTGCCTC TCCTAGAATT GCAAGATATT ATGACCAGTG TGTCTGGCCG CATTCCCCCC AATGTGGAGA AGTCTATCAA GAAGGAAATG GCTCAGTATG CTAGCAACAT CACATCAGTC CTCTGTCAGT TTCCCAGCCA GCAGATTGCA AACATCCTAG ATAGCCATGC AGCTACATTG AACCGGAAAT CTGAACGGGA AGTCTTCTTT ATGAATACTC AGAGCATTGT TCAGCTGGTA CAGAGGTACC GAAGTGGCAT CCGAGGCCAC ATGAAGGCTG TGGTGATGGA TCTGCTCCGG CAGTACCTGC GAGTAGAGAC ACAATTCCAG AATGGTCACT ATGACAAATG TGTATTCGCC CTCCGAGAAG AGAATAAAAG TGACATGAAC ACTGTACTGA ACTACATCTT CTCTCACGCT CAAGTCACCA AGAAGAATCT TCTGGTCACA ATGCTTATTG ATCAGTTGTG TGGCCGGGAC CCTACTCTCA CTGATGAGCT GCTGAATATT CTCACAGAGC TAACTCAACT CAGTAAGACC ACCAATGCCA AAGTAGCACT TCGAGCACGC CAGGTTCTTA TTGCCTCCCA TTTGCCATCA TATGAGCTTC GCCATAACCA AGTAGAGTCT ATCTTCCTAT CAGCTATTGA CATGTATGGA CATCAATTTT GCATTGAGAA CCTGCAGAAA CTCATCCTAT CAGAAACATC TATTTTTGAT GTCCTACCAA ACTTCTTCTA TCACAGCAAC CAAGTAGTGA GGATGGCAGC TCTGGAGGTG TATGTTCGAA GGGCTTATAT TGCCTATGAA CTTAACAGCG TACAACACCG CCAGCTTAAG GACAACACCT GTGTGGTGGA ATTCCAGTTC ATGCTGCCCA CATCTCATCC AAACAGAGGG AACATCCCTA CGCTAAACAG AATGTCCTTC TCCTCCAACC TCAACCACTA TGGCATGACC CATGTAGCTA GTGTCAGCGA TGTACTGTTG GACAACTCAT TCACTCCACC TTGTCAGCGG ATGGGCGGAA TGGTCTCTTT TCGGACTTTT GAAGATTTTG TCAGGATCTT TGATGAAGTG ATGGGCTGCT TCTCTGACTC CCCACCCCAG AGTCCCACAT TCCCTGAGGC AGGTCACACG TCTCTTTATG ATGAGGATAA GGTTCCCAGG GATGAACCAA TTCACATTCT CAATGTGGCT ATCAAGACTG ACTGTGATAT TGAGGATGAC AGGCTGGCAG CTATGTTCAG AGAATTTACC CAGCAAAATA AAGCTACCCT GGTTGACCAT GGGATCCGGC GCCTTACTTT CCTGGTTGCA CAAAAGGATT TCAGAAAGCA GGTCAACTAT GAGGTGGATC GGAGATTTCA TAGAGAATTC CCTAAATTTT TTACATTCCG AGCAAGGGAT AAGTTTGAGG AGGATCGTAT CTATCGTCAT CTGGAGCCTG CTCTGGCTTT CCAGTTAGAG CTGAACCGGA TGAGAAATTT TGACCTCACT GCCATTCCAT GTGCTAATCA CAAGATGCAC CTGTATCTCG GGGCAGCCAA GGTGGAAGTG GGCACAGAAG TGACAGACTA CAGGTTCTTT GTTCGTGCAA TCATCAGGCA TTCTGATCTG GTCACCAAGG AAGCTTCTTT TGAATATCTG CAAAATGAAG GGGAGCGGCT ACTCCTGGAA GCCATGGATG AGTTGGAAGT TGCTTTTAAC AATACAAATG TCCGCACTGA CTGTAACCAC ATCTTCCTCA ACTTTGTGCC CACGGTTATC ATGGACCCAT CAAAGATTGA GGAATCCGTG CGGAGCATGG TAATGCGGTA TGGAAGTCGC CTGTGGAAAT TGCGCGTCCT CCAGGCAGAA CTGAAAATCA ACATTCGCCT GACGCCAACT GGAAAAGCAA TTCCCATCCG CCTCTTCCTG ACAAACGAGT CTGGCTATTA CTTGGATATC AGCCTATACA AGGAAGTGAC TGACTCCAGG ACAGCACAGA TCATGTTTCA GGCATATGGA GACAAACAGG GACCACTGCA TGGAATGTTA ATCAATACTC CATATGTGAC CAAAGACCTG CTGCAATCAA AGAGGTTCCA GGCACAATCC TTAGGGACAA CATACATATA TGATATCCCA GAGATGTTTC GGCAGTCCCT GATCAAACTC TGGGAGTCTA TGTCCACTCA AGCATTTCTT CCATCTCCCC CTCTGCCTTC TGACATGCTG ACTTACACTG AACTGGTACT GGATGATCAA GGTCAGCTGG TCCACATGAA CAGGCTTCCA GGAGGAAATG AGATTGGCAT GGTAGCTTGG AAAATGACCT TTAAAAGTCC TGAATATCCA GAAGGCCGAG ATATCATTGT TATTGGCAAT GACATCACAT ACCGAATTGG GTCCTTTGGG CCTCAAGAGG ATTTGTTATT TCTCAGAGCT TCCGAACTTG CTAGGGCAGA AGGTATTCCA CGCATCTATG TATCAGCCAA CAGTGGAGCA AGAATCGGAC TGGCAGAAGA AATTCGCCAT ATGTTTCATG TGGCCTGGGT AGATCCTGAG GATCCTTACA AGGGATACAG GTATTTATAT CTGACTCCTC AAGATTATAA GAGAGTCAGT GCTCTCAACT CTGTCCATTG TGAACACGTG GAAGATGAAG GAGAATCCAG GTACAAGATA ACAGATATTA TTGGGAAAGA AGAGGGAATT GGACCCGAGA ACCTTCGAGG TTCTGGAATG ATTGCTGGAG AATCCTCATT GGCCTATAAT GAGATCATTA CCATCAGCCT GGTGACGTGC CGGGCCATTG GGATTGGGGC TTACCTTGTC CGGCTGGGAC AGAGAACCAT CCAGGTTGAG AATTCTCACT TAATTCTAAC AGGAGCTGGA GCCCTCAACA AAGTCCTCGG GCGGGAAGTG TACACCTCCA ATAACCAGCT GGGGGGCATC CAGATTATGC ACAACAATGG GGTGACCCAC TGCACTGTGT GTGATGACTT TGAAGGGGTT TTCACTGTCC TGCACTGGCT GTCTTACATG CCCAAGAGCG TGCACAGTTC AGTTCCTCTT CTGAACTCAA AGGATCCTAT AGACAGAATC ATCGAGTTTG TTCCCACAAA GACCCCATAC GATCCTCGAT GGATGCTAGC AGGCCGTCCT CACCCAACCC AAAAAGGTCA GTGGTTGAGT GGCTTTTTTG ACTATGGATC TTTCTCAGAG ATTATGCAGC CCTGGGCACA GACTGTGGTG GTTGGTAGAG CCAGGCTAGG AGGAATACCT GTGGGAGTTG TTGCTGTAGA AACCCGAACA GTAGAACTAA GTATCCCAGC TGATCCAGCA AACCTGGATT CTGAAGCCAA GATAATCCAG CAGGCTGGCC AGGTTTGGTT CCCAGATTCT GCGTTTAAGA CGTATCAGGC CATCAAGGAC TTCAACCGGG AAGGGCTGCC TCTGATGGTC TTTGCCAACT GGAGAGGCTT CTCTGGTGGA ATGAAAGATA TGTACGACCA AGTGCTGAAG TTTGGTGCTT ACATTGTGGA TGGCTTGAGG GAGTGCTGCC AGCCTGTGCT GGTTTACATT CCTCCCCAGG CTGAGCTGCG GGGTGGCTCC TGGGTGGTGA TTGACTCCTC CATCAACCCC CGGCACATGG AGATGTATGC TGACCGAGAA AGCAGGGGAT CTGTTCTGGA GCCAGAAGGG ACAGTAGAAA TCAAATTCCG CAGAAAGGAT CTGGTGAAAA CCATGCGTCG GGTGGACCCA GTCTACATCC ACTTGGCTGA GCGATTGGGG ACCCCAGAGC TAAGCACAGC TGAGCGGAAG GAGTTGGAGA ACAAGTTGAA GGAGCGGGAG GAATTCCTAA TTCCCATTTA CCATCAGGTA GCCGTGCAGT TTGCTGACTT GCACGACACA CCAGGCCGGA TGCAGGAGAA GGGTGTTATT AGCGATATCC TGGATTGGAA AACATCCCGT ACCTTCTTCT ACTGGCGGCT GAGGCGTCTT CTGCTGGAGG ACCTGGTCAA GAAGAAAATC CACAATGCCA ACCCTGAGCT GACTGATGGC CAGATTCAAG CCATGTTAAG GCGCTGGTTT GTGGAAGTGG AAGGAACAGT GAAGGCTTAT GTTTGGGACA ATAATAAGGA TCTGGCGGAG TGGCTAGAGA AACAGCTGAC AGAGGAGGAT GGTGTTCACT CGGTAATAGA GGAAAACATC AAATGCATCA GCAGAGACTA CGTCCTCAAG CAAATCCGCA GCTTGGTCCA GGCCAATCCA GAGGTTGCCA TGGATTCCAT CATCCATATG ACGCAGCACA TATCACCCAC TCAGCGAGCA GAAGTCATAC GGATCCTCTC CACAATGGAT TCCCCTTCCA CGGAATTCGG GGGTGGAGGC TCTGGCGGAG GTGGATCAAT GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATAGGATCC ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC ATGCAT
