pSV40-Rluc-HSVTK-Fluc-ITGB6(human)-3UTR
$84.00
In stock
SKU
PL46773
100μg liquid plasmid(Instantly):$105
| name | pSV40-Rluc-HSVTK-Fluc-ITGB6(human)-3UTR | another name | apSV40-Rluc-HSVTK-Fluc-ITGB6(human)-3UTR |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Luciferase Report | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | psiCHECK-2-F：GAAGTTCCCTAACACCGAGT | |
| 3′ sequencing primer | Psicheck-2-R：CCCCCGCGAGGTCCGAAGAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGTG AGACTCTAAT TTCAAACATT TTTCAAATGC AAATCAGGCC CCCATGAATC ATTTGATGAT TTTTTGAAGC ATTAATGCAA CAGAGTTAGT ATTCCTCAAA TGATCCCCAA AGTCATCTCT TTGCTAAGGA TATTTCTCTT CTTGATTATT TTGAAACTGC CAACAGTCTG TCACCTTCAT GTAGAGGATG ACTTATCTGC GCGGCCGCTG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAGGATC TAAATGAGTC TTCGGACCTC GCGGGGGCCG CTTAAGCGGT GGTTAGGGTT TGTCTGACGC GGGGGGAGGG GGAAGGAACG AAACACTCTC ATTCGGAGGC GGCTCGGGGT TTGGTCTTGG TGGCCACGGG CACGCAGAAG AGCGCCGCGA TCCTCTTAAG CACCCCCCCG CCCTCCGTGG AGGCGGGGGT TTGGTCGGCG GGTGGTAACT GGCGGGCCGC TGACTCGGGC GGGTCGCGCG CCCCAGAGTG TGACCTTTTC GGTCTGCTCG CAGACCCCCG GGCGGCGCCG CCGCGGCGGC GACGGGCTCG CTGGGTCCTA GGCTCCATGG GGACCGTATA CGTGGACAGG CTCTGGAGCA TCCGCACGAC TGCGGTGATA TTACCGGAGA CCTTCTGCGG GACGAGCCGG GTCACGCGGC TGACGCGGAG CGTCCGTTGG GCGACAAACA CCAGGACGGG GCACAGGTAC ACTATCTTGT CACCCGGAGG CGCGAGGGAC TGCAGGAGCT TCAGGGAGTG GCGCAGCTGC TTCATCCCCG TGGCCCGTTG CTCGCGTTTG CTGGCGGTGT CCCCGGAAGA AATATATTTG CATGTCTTTA GTTCTATGAT GACACAAACC CCGCCCAGCG TCTTGTCATT GGCGAATTCG AACACGCAGA TGCAGTCGGG GCGGCGCGGT CCCAGGTCCA CTTCGCATAT TAAGGTGACG CGTGTGGCCT CGAACACCGA GCGACCCTGC AGCGACCCGC TTAAAAGCTT GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGCCGATGC TAAGAACATT AAGAAGGGCC CTGCTCCCTT CTACCCTCTG GAGGATGGCA CCGCTGGCGA GCAGCTGCAC AAGGCCATGA AGAGGTATGC CCTGGTGCCT GGCACCATTG CCTTCACCGA TGCCCACATT GAGGTGGACA TCACCTATGC CGAGTACTTC GAGATGTCTG TGCGCCTGGC CGAGGCCATG AAGAGGTACG GCCTGAACAC CAACCACCGC ATCGTGGTGT GCTCTGAGAA CTCTCTGCAG TTCTTCATGC CAGTGCTGGG CGCCCTGTTC ATCGGAGTGG CCGTGGCCCC TGCTAACGAC ATTTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATTT CTCAGCCTAC CGTGGTGTTC GTGTCTAAGA AGGGCCTGCA GAAGATCCTG AACGTGCAGA AGAAGCTGCC TATCATCCAG AAGATCATCA TCATGGACTC TAAGACCGAC TACCAGGGCT TCCAGAGCAT GTACACATTC GTGACATCTC ATCTGCCTCC TGGCTTCAAC GAGTACGACT TCGTGCCAGA GTCTTTCGAC AGGGACAAAA CCATTGCCCT GATCATGAAC AGCTCTGGGT CTACCGGCCT GCCTAAGGGC GTGGCCCTGC CTCATCGCAC CGCCTGTGTG CGCTTCTCTC ACGCCCGCGA CCCTATTTTC GGCAACCAGA TCATCCCCGA CACCGCTATT CTGAGCGTGG TGCCATTCCA CCACGGCTTC GGCATGTTCA CCACCCTGGG CTACCTGATT TGCGGCTTTC GGGTGGTGCT GATGTACCGC TTCGAGGAGG AGCTGTTCCT GCGCAGCCTG CAAGACTACA AAATTCAGTC TGCCCTGCTG GTGCCAACCC TGTTCAGCTT CTTCGCTAAG AGCACCCTGA TCGACAAGTA CGACCTGTCT AACCTGCACG AGATTGCCTC TGGCGGCGCC CCACTGTCTA AGGAGGTGGG CGAAGCCGTG GCCAAGCGCT TTCATCTGCC AGGCATCCGC CAGGGCTACG GCCTGACCGA GACAACCAGC GCCATTCTGA TTACCCCAGA GGGCGACGAC AAGCCTGGCG CCGTGGGCAA GGTGGTGCCA TTCTTCGAGG CCAAGGTGGT GGACCTGGAC ACCGGCAAGA CCCTGGGAGT GAACCAGCGC GGCGAGCTGT GTGTGCGCGG CCCTATGATT ATGTCCGGCT ACGTGAATAA CCCTGAGGCC ACAAACGCCC TGATCGACAA GGACGGCTGG CTGCACTCTG GCGACATTGC CTACTGGGAC GAGGACGAGC ACTTCTTCAT CGTGGACCGC CTGAAGTCTC TGATCAAGTA CAAGGGCTAC CAGGTGGCCC CAGCCGAGCT GGAGTCTATC CTGCTGCAGC ACCCTAACAT TTTCGACGCC GGAGTGGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CTGCCGCCGT CGTCGTGCTG GAACACGGCA AGACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTGACA ACCGCCAAGA AGCTGCGCGG CGGAGTGGTG TTCGTGGACG AGGTGCCCAA GGGCCTGACC GGCAAGCTGG ACGCCCGCAA GATCCGCGAG ATCCTGATCA AGGCTAAGAA AGGCGGCAAG ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGGCTCGA C
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