pPLPR-E(phiX174)-Chl
$99.00
In stock
SKU
PL46752
100μg liquid plasmid(Instantly):$142
| name | pPLPR-E(phiX174)-Chl | another name | apPLPR-E(phiX174)-Chl |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | PL,PR | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATATCTAACA CCGTGCGTGT TGACTATTTT ACCTCTGGCG GTGATAATGG TTGCATGTAC TAAGGAGGTT GTATGGAACA ACGCATAACC CTGAAAGATT ATGCAATGCG CTTTGGGCAA ACCAAGACAG CTAAAGATCT CTCACCTACC AAACAATGCC CCCCTGCAAA AAATAAATTC ATATAAAAAA CATACAGATA ACCATCTGCG GTGATAAATT ATCTCTGGCG GTGTTGACAT AAATACCACT GGCGGTGATA CTGAGCACAT CAGCAGGACG CACTGACCAC CATGAAGGTG ACGCTCTTAA AAATTAAGCC CTGAAGAAGG GCAGCATTCA AAGCAGAAGG CTTTGGGGTG TGTGATACGA AACGAAGCAT TGGTTAAAAA TTAAGGAGGA ATTCCCGGGG ATCCATGGTA CGCTGGACTT TGTGGGATAC CCTCGCTTTC CTGCTCCTGT TGAGTTTATT GCTGCCGTCA TTGCTTATTA TGTTCATCCC GTCAACATTC AAACGGCCTG TCTCATCATG GAAGGCGCTG AATTTACGGA AAACATTATT AATGGCGTCG AGCGTCCGGT TAAAGCCGCT GAATTGTTCG CGTTTACCTT GCGTGTACGC GCAGGAAACA CTGACGTTCT TACTGACGCA GAAGAAAACG TGCGTCAAAA ATTACGTGCG GAAGGAGTGA GTCGACCTGC AGCCAAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTGAT CGGCACGTAA GAGGTTCCAA CTTTCACCAT AATGAAATAA GATCACTACC GGGCGTATTT TTTGAGTTAT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC CGTCTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCTTATCT TTCCCTTTAT TTTTGCTGCG GTAAGTCGCA TAAAAACCAT TCTTCATAAT TCAATCCATT TACTATGTTA TGTTCTGAGG GGAGTGAAAA TTCCCCTAAT TCGATGAAGA TTCTTGCTCA ATTGTTATCA GCTATGCGCC GACCAGAACA CCTTGCCGAT CAGCCAAACG TCTCTTCAGG CCACTGACTA GCGATAACTT TCCCCACAAC GGAACAACTC TCATTGCATG GGATCATTGG GTACTGTGGG TTTAGTGGTT GTAAAAACAC CTGACCGCTA TCCCTGATCA GTTTCTTGAA GGTAAACTCA TCACCCCCAA GTCTGGCTAT GCAGAAATCA CCTGGCTCAA CAGCCTGCTC AGGGTCAACG AGAATTAACA TTCCGTCAGG AAAGCTTGGC TTGGAGCCTG TTGGTGCGGT CATGGAATTA CCTTCAACCT CAAGCCAGAA TGCAGAATCA CTGGCTTTTT TGGTTGTGCT TACCCATCTC TCCGCATCAC CTTTGGTAAA GGTTCTAAGC TTAGGTGAGA ACATCCCTGC CTGAACATGA GAAAAAACAG GGTACTCATA CTCACTTCTA AGTGACGGCT GCATACTAAC CGCTTCATAC ATCTCGTAGA TTTCTCTGGC GATTGAAGGG CTAAATTCTT CAACGCTAAC TTTGAGAATT TTTGTAAGCA ATGCGGCGTT ATAAGCATTT AATGCATTGA TGCCATTAAA TAAAGCACCA ACGCCTGACT GCCCCATCCC CATCTTGTCT GCGACAGATT CCTGGGATAA GCCAAGTTCA TTTTTCTTTT TTTCATAAAT TGCTTTAAGG CGACGTGCGT CCTCAAGCTG CTCTTGTGTT AATGGTTTCT TTTTTGTGCT CATACGTTAA ATCTATCACC GCAAGGGATA A
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