NM_001897.5;CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;MSK16; NG2
100μg liquid plasmid(Instantly):$163
| name | pENTR223.1-CSPG4(human) | another name | apENTR223.1-CSPG4(human) |
| Insert Size (bp) | 6967bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGCAGTCC GGGCCGCGGC CCCCACTTCC AGCCCCCGGC CTGGCCTTGG CTTTGACCCT GACTATGTTG GCCAGACTTG CATCCGCGGC TTCCTTCTTC GGTGAGAACC ACCTGGAGGT GCCTGTGGCC ACGGCTCTGA CCGACATAGA CCTGCAGCTG CAGTTCTCCA CGTCCCAGCC CGAAGCCCTC CTTCTCCTGG CAGCAGGCCC AGCTGACCAC CTCCTGCTGC AGCTCTACTC TGGACGCCTG CAGGTCAGAC TTGTTCTGGG CCAGGAGGAG CTGAGGCTGC AGACTCCAGC AGAGACGCTG CTGAGTGACT CCATCCCCCA CACTGTGGTG CTGACTGTCG TAGAGGGCTG GGCCACGTTG TCAGTCGATG GGTTTCTGAA CGCCTCCTCA GCAGTCCCAG GAGCCCCCCT AGAGGTCCCC TATGGGCTCT TTGTTGGGGG CACTGGGACC CTTGGCCTGC CCTACCTGAG GGGAACCAGC CGACCCCTGA GGGGTTGCCT CCATGCAGCC ACCCTCAATG GCCGCAGCCT CCTCCGGCCT CTGACCCCCG ATGTGCATGA GGGCTGTGCT GAAGAGTTTT CTGCCAGTGA TGATGTGGCC CTGGGCTTCT CTGGGCCCCA CTCTCTGGCT GCCTTCCCTG CCTGGGGCAC TCAGGACGAA GGAACCCTAG AGTTTACACT CACCACACAG AGCCGGCAGG CACCCTTGGC CTTCCAGGCA GGGGGCCGGC GTGGGGACTT CATCTATGTG GACATATTTG AGGGCCACCT GCGGGCCGTG GTGGAGAAGG GCCAGGGTAC CGTATTGCTC CACAACAGTG TGCCTGTGGC CGATGGGCAG CCCCATGAGG TCAGTGTCCA CATCAATGCT CACCGGCTGG AAATCTCCGT GGACCAGTAC CCTACGCATA CTTCGAACCG AGGAGTCCTC AGCTACCTGG AGCCACGGGG CAGTCTCCTT CTCGGGGGGC TGGATGCAGA GGCCTCTCGT CACCTCCAGG AACACCGCCT GGGCCTGACA CCAGAGGCCA CCAATGCCTC CCTGCTGGGC TGCATGGAAG ACCTCAGTGT CAATGGCCAG AGGCGGGGGC TGCGGGAAGC TTTGCTGACG CGCAACATGG CAGCCGGCTG CAGGCTGGAG GAGGAGGAGT ATGAGGACGA TGCCTATGGA CATTATGAAG CTTTCTCCAC CCTGGCCCCT GAGGCTTGGC CAGCCATGGA GCTGCCTGAG CCATGCGTGC CTGAGCCAGG GCTGCCTCCT GTCTTTGCCA ATTTCACCCA GCTGCTGACT ATCAGCCCAC TGGTGGTGGC CGAGGGGGGC ACAGCCTGGC TTGAGTGGAG GCATGTGCAG CCCACGCTGG ACCTGATGGA GGCTGAGCTG CGCAAATCCC AGGTGCTGTT CAGCGTGACC CGAGGGGCAC GCCATGGCGA GCTCGAGCTG GACATCCCGG GAGCCCAGGC ACGAAAAATG TTCACCCTCC TGGACGTGGT GAACCGCAAG GCCCGCTTCA TCCACGATGG CTCTGAGGAC ACCTCCGACC AGCTGGTGCT GGAGGTGTCG GTGACGGCTC GGGTGCCCAT GCCCTCATGC CTTCGGAGGG GCCAAACATA CCTCCTGCCC ATCCAGGTCA ACCCTGTCAA TGACCCACCC CACATCATCT TCCCACATGG CAGCCTCATG GTGATCCTGG AACACACGCA GAAGCCGCTG GGGCCTGAGG TTTTCCAGGC CTATGACCCG GACTCTGCCT GTGAGGGCCT CACCTTCCAG GTCCTTGGCA CCTCCTCTGG CCTCCCCGTG GAGCGCCGAG ACCAGCCTGG GGAGCCGGCG ACCGAGTTCT CCTGCCGGGA GTTGGAGGCC GGCAGCCTAG TCTATGTCCA CCGCGGTGGT CCTGCACAGG ACTTGACGTT CCGGGTCAGC GATGGACTGC AGGCCAGCCC CCCGGCCACG CTGAAGGTGG TGGCCATCCG GCCGGCCATA CAGATCCACC GCAGCACAGG GTTGCGACTG GCCCAAGGCT CTGCCATGCC CATCTTGCCC GCCAACCTGT CGGTGGAGAC CAATGCCGTG GGGCAGGATG TGAGCGTGCT GTTCCGCGTC ACTGGGGCCC TGCAGTTTGG GGAGCTGCAG AAGCAGGGGG CAGGTGGGGT GGAGGGTGCT GAGTGGTGGG CCACACAGGC GTTCCACCAG CGGGATGTGG AGCAGGGCCG CGTGAGGTAC CTGAGCACTG ACCCACAGCA CCACGCTTAC GACACCGTGG AGAACCTGGC CCTGGAGGTG CAGGTGGGCC AGGAGATCCT GAGCAATCTG TCCTTCCCAG TGACCATCCA GAGAGCCACT GTGTGGATGC TGCGGCTGGA GCCACTGCAC ACTCAGAACA CCCAGCAGGA GACCCTCACC ACAGCCCACC TGGAGGCCAC CCTGGAGGAG GCAGGCCCAA GCCCCCCAAC CTTCCATTAT GAGGTGGTTC AGGCTCCCAG GAAAGGCAAC CTTCAACTAC AGGGCACAAG GCTGTCAGAT GGCCAGGGCT TCACCCAGGA TGACATACAG GCTGGCCGGG TGACCTATGG GGCCACAGCA CGTGCCTCAG AGGCAGTCGA GGACACCTTC CGTTTCCGTG TCACAGCTCC ACCATATTTC TCCCCACTCT ATACCTTCCC CATCCACATT GGTGGTGACC CAGATGCGCC TGTCCTCACC AATGTCCTCC TCGTGGTGCC TGAGGGTGGT GAGGGTGTCC TCTCTGCTGA CCACCTCTTT GTCAAGAGTC TCAACAGTGC CAGCTACCTC TATGAGGTCA TGGAGCGGCC CCGCCATGGG AGGTTGGCTT GGCGTGGGAC ACAGGACAAG ACCACTATGG TGACATCCTT CACCAATGAA GACCTGTTGC GTGGCCGGCT GGTCTACCAG CATGATGACT CCGAGACCAC AGAAGATGAT ATCCCATTTG TTGCTACCCG CCAGGGCGAG AGCAGTGGTG ACATGGCCTG GGAGGAGGTA CGGGGTGTCT TCCGAGTGGC CATCCAGCCC GTGAATGACC ACGCCCCTGT GCAGACCATC AGCCGGATCT TCCATGTGGC CCGGGGTGGG CGGCGGCTGC TGACTACAGA CGACGTGGCC TTCAGCGATG CTGACTCGGG CTTTGCTGAC GCCCAGCTGG TGCTTACCCG CAAGGACCTC CTCTTTGGCA GTATCGTGGC CGTAGATGAG CCCACGCGGC CCATCTACCG CTTCACCCAG GAGGACCTCA GGAAGAGGCG AGTACTGTTC GTGCACTCAG GGGCTGACCG TGGCTGGATC CAGCTGCAGG TGTCCGACGG GCAACACCAG GCCACTGCGC TGCTGGAGGT GCAGGCCTCG GAACCCTACC TCCGTGTGGC CAACGGCTCC AGCCTTGTGG TCCCTCAAGG AGGCCAGGGC ACCATCGACA CGGCCGTGCT CCACCTGGAC ACCAACCTCG ACATCCGCAG TGGGGATGAG GTCCACTACC ACGTCACAGC TGGCCCTCGC TGGGGACAGC TAGTCCGGGC TGGTCAGCCA GCCACAGCCT TCTCCCAGCA GGACCTGCTG GATGGGGCCG TTCTCTATAG CCACAATGGC AGCCTCAGCC CCCGCGACAC CATGGCCTTC TCCGTGGAAG CAGGGCCAGT GCACACGGAT GCCACCCTAC AAGTGACCAT TGCCCTAGAG GGCCCACTGG CCCCACTGAA GCTGGTCCGG CACAAGAAGA TCTACGTCTT CCAGGGAGAG GCAGCTGAGA TCAGAAGGGA CCAGCTGGAG GCAGCCCAGG AGGCAGTGCC ACCTGCAGAC ATCGTATTCT CAGTGAAGAG CCCACCGAGT GCCGGCTACC TGGTGATGGT GTCGCGTGGC GCCTTGGCAG ATGAGCCACC CAGCCTGGAC CCTGTGCAGA GCTTCTCCCA GGAGGCAGTG GACACAGGCA GGGTCCTGTA CCTGCACTCC CGCCCTGAGG CCTGGAGCGA TGCCTTCTCG CTGGATGTGG CCTCAGGCCT GGGTGCTCCC CTCGAGGGCG TCCTTGTGGA GCTGGAGGTG CTGCCCGCTG CCATCCCACT AGAGGCGCAA AACTTCAGCG TCCCTGAGGG TGGCAGCCTC ACCCTGGCCC CTCCACTGCT CCGTGTCTCC GGGCCCTACT TCCCCACTCT CCTGGGCCTC AGCCTGCAGG TGCTGGAGCC ACCCCAGCAT GGAGCCCTGC AGAAGGAGGA CGGACCTCAA GCCAGGACCC TCAGCGCCTT CTCCTGGAGA ATGGTGGAAG AGCAGCTGAT CCGCTACGTG CATGACGGGA GCGAGACACT GACAGACAGT TTTGTCCTGA TGGCTAATGC CTCCGAGATG GATCGCCAGA GCCATCCTGT GGCCTTCACT GTCACTGTCC TGCCTGTCAA TGACCAACCC CCCATCCTCA CTACAAACAC AGGCCTGCAG ATGTGGGAGG GGGCCACTGC GCCCATCCCT GCGGAGGCTC TGAGGAGCAC GGACGGCGAC TCTGGGTCTG AGGATCTGGT CTACACCATC GAGCAGCCCA GCAACGGGCG GGTAGTGCTG CGGGGGGCGC CGGGCACTGA GGTGCGCAGC TTCACGCAGG CCCAGCTGGA CGGCGGGCTC GTGCTGTTCT CACACAGAGG AACCCTGGAT GGAGGCTTCC GCTTCCGCCT CTCTGACGGC GAGCACACTT CCCCCGGACA CTTCTTCCGA GTGACGGCCC AGAAGCAAGT GCTCCTCTCG CTGAAGGGCA GCCAGACACT GACTGTCTGC CCAGGGTCCG TCCAGCCACT CAGCAGTCAG ACCCTCAGGG CCAGCTCCAG CGCAGGCACT GACCCCCAGC TCCTGCTCTA CCGTGTGGTG CGGGGCCCCC AGCTAGGCCG GCTGTTCCAC GCCCAGCAGG ACAGCACAGG GGAGGCCCTG GTGAACTTCA CTCAGGCAGA GGTCTACGCT GGGAATATTC TGTATGAGCA TGAGATGCCC CCCGAGCCCT TTTGGGAGGC CCATGATACC CTAGAGCTCC AGCTGTCCTC GCCGCCTGCC CGGGACGTGG CCGCCACCCT TGCTGTGGCT GTGTCTTTTG AGGCTGCCTG TCCCCAGCGC CCCAGCCACC TCTGGAAGAA CAAAGGTCTC TGGGTCCCCG AGGGCCAGCG GGCCAGGATC ACCGTGGCTG CTCTGGATGC CTCCAATCTC TTGGCCAGCG TTCCATCACC CCAGCGCTCA GAGCATGATG TGCTCTTCCA GGTCACACAG TTCCCCAGCC GGGGCCAGCT GTTGGTGTCC GAGGAGCCCC TCCATGCTGG GCAGCCCCAC TTCCTGCAGT CCCAGCTGGC TGCAGGGCAG CTAGTGTATG CCCACGGCGG TGGGGGCACC CAGCAGGATG GCTTCCACTT TCGTGCCCAC CTCCAGGGGC CAGCAGGGGC CTCCGTGGCT GGACCCCAAA CCTCAGAGGC CTTTGCCATC ACGGTGAGGG ATGTAAATGA GCGGCCCCCT CAGCCACAGG CCTCTGTCCC ACTCCGGCTC ACCCGAGGCT CTCGTGCCCC CATCTCCCGG GCCCAGCTGA GTGTGGTGGA CCCAGACTCA GCTCCTGGGG AGATTGAGTA CGAGGTCCAG CGGGCACCCC ACAACGGCTT CCTCAGCCTG GTGGGTGGTG GCCTGGGGCC CGTGACCCGC TTCACGCAAG CCGATGTGGA TTCAGGGCGG CTGGCCTTCG TGGCCAACGG GAGCAGCGTG GCAGGCATCT TCCAGCTGAG CATGTCTGAT GGGGCCAGCC CACCCCTGCC CATGTCCCTG GCTGTGGACA TCCTACCATC CGCCATCGAG GTGCAGCTGC GGGCACCCCT GGAGGTGCCC CAAGCTTTGG GGCGCTCCTC ACTGAGCCAG CAGCAGCTCC GGGTGGTTTC AGATCGGGAG GAGCCAGAGG CAGCATACCG CCTCATCCAG GGACCCCAGT ATGGGCATCT CCTGGTGGGC GGGCGGCCCA CCTCGGCCTT CAGCCAATTC CAGATAGACC AGGGCGAGGT GGTCTTTGCC TTCACCAACT TCTCCTCCTC TCATGACCAC TTCAGAGTCC TGGCACTGGC TAGGGGTGTC AATGCATCAG CCGTAGTGAA CGTCACTGTG AGGGCTCTGC TGCATGTGTG GGCAGGTGGG CCATGGCCCC AGGGTGCCAC CCTGCGCCTG GACCCCACCG TCCTAGATGC TGGCGAGCTG GCCAACCGCA CAGGCAGTGT GCCGCGCTTC CGCCTCCTGG AGGGACCCCG GCATGGCCGC GTGGTCCGCG TGCCCCGAGC CAGGACGGAG CCCGGGGGCA GCCAGCTGGT GGAGCAGTTC ACTCAGCAGG ACCTTGAGGA CGGGAGGCTG GGGCTGGAGG TGGGCAGGCC AGAGGGGAGG GCCCCCGGCC CCGCAGGTGA CAGTCTCACT CTGGAGCTGT GGGCACAGGG CGTCCCGCCT GCTGTGGCCT CCCTGGACTT TGCCACTGAG CCTTACAATG CTGCCCGGCC CTACAGCGTG GCCCTGCTCA GTGTCCCCGA GGCCGCCCGG ACGGAAGCAG GGAAGCCAGA GAGCAGCACC CCCACAGGCG AGCCAGGCCC CATGGCATCC AGCCCTGAGC CCGCTGTGGC CAAGGGAGGC TTCCTGAGCT TCCTTGAGGC CAACATGTTC AGCGTCATCA TCCCCATGTG CCTGGTACTT CTGCTCCTGG CGCTCATCCT GCCCCTGCTC TTCTACCTCC GAAAACGCAA CAAGACGGGC AAGCATGACG TCCAGGTCCT GACTGCCAAG CCCCGCAACG GCCTGGCTGG TGACACCGAG ACCTTTCGCA AGGTGGAGCC AGGCCAGGCC ATCCCGCTCA CAGCTGTGCC TGGCCAGGGG CCCCCTCCAG GAGGCCAGCC TGACCCAGAG CTGCTGCAGT TCTGCCGGAC ACCCAACCCT GCCCTTAAGA ATGGCCAGTA CTGGGTGTCA GGCCTCATGG GCCCAGCTTT CTTGTACAAA GTTGGCATTA TAAAAAATAA TTGCTCATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC ATGCCTCCTC TAGACCAGCC AGGACAGAAA TGCCTCGACT TCGCTGCTGC CCAAGGTTGC CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTGGACAT AAGCCTGTTC GGTTCGTAAG CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT CAACTATCAG AGGTAGTTGG CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA CATTTGTACG GCTCCGCAGT GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG GTTACGGTGA CCGTAAGGCT TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG GAAACTTCGG CTTCCCCTGG AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT GTGCACGACG ACATCATTCC GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA TGGCAGCGCA ATGACATTCT TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG GCTATCTTGC TGACAAAAGC AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG GAACTCTTTG ATCCGGTTCC TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG CTATGGAACT CGCCGCCCGA CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC CGCATTTGGT ACAGCGCAGT AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG GCAATGGAGC GCCTGCCGGC CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT CTTGGACAAG AAGAAGATCG CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC TACGTGAAAG GCGAGATCAC CAAGGTAGTC GGCAAATAAC CCTCGAGCCA CCCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAA
