NM_001320714.2(1synonymous mutations );21orf5; C21orf5; DOPEY2
100μg liquid plasmid(Instantly):$163
| name | pENTR223.1-DOP1B(human)(1synonymous mutations) | another name | apENTR223.1-DOP1B(human)(1synonymous mutations) |
| Insert Size (bp) | 6895bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGGATCCA GAAGAGCAGG AGCTCTTAAA TGATTACAGA TACAGAAGCT ACTCTTCAGT GATTGAAAAG GCTTTGAGAA ATTTTGAGTC CTCGAGTGAA TGGGCGGATC TCATATCTTC ACTTGGCAAA CTCAACAAGG CTCTTCAGAG TAACCTGAGG TACTCCTTGT TGCCAAGACG GCTCCTCATC AGCAAAAGAT TAGCTCAGTG TTTGCACCCT GCCCTGCCCA GTGGTGTCCA CTTAAAAGCT CTGGAAACCT ACGAGATTAT CTTTAAAATC GTGGGGACCA AATGGCTGGC CAAGGACTTG TTTCTGTACA GCTGCGGGTT ATTTCCTCTC CTGGCACACG CGGCGGTGTC GGTGAGGCCG GTGCTGCTCA CCCTGTACGA GAAGTACTTC CTCCCACTGC AGAAGCTGCT CCTGCCCAGT CTGCAGGCCT TCATCGTGGG CCTGCTGCCC GGCCTTGAAG AGGGCTCCGA GATCTCCGAC AGAACGGATG CTCTGCTCCT GAGACTGTCG CTGGTGGTTG GCAAAGAGGT GTTTTACACC GCCCTCTGGG GGAGCGTCCT GGCCAGCCCG TCCATCCGCC TCCCTGCCTC AGTCTTCGTG GTGGGCCACA TCAACAGGGA TGCCCCCGGC CGGGAGCAGA AGTACATGCT GGGGACCAAT CACCAACTCA CGGTGAAGTC TTTGCGTGCC TCCCTGTTGG ACTCAAATGT TCTTGTGCAA AGAAATAATC TGGAAATCGT TCTGTTTTTC TTCCCATTTT ATACCTGTCT GGATTCCAAT GAGAGAGCCA TCCCCCTCCT CAGATCTGAC ATCGTGCGCA TTCTCTCAGC CGCCACCCAG ACCCTACTGA GAAGGGACAT GTCCCTGAAC AGAAGACTGT ATGCATGGTT ACTAGGCTCA GACATAAAAG GAAATACCGT TGTGCCAGAA TCTGAAATCT CAAATTCTTA TGAAGACCAG TCGTCTTATT TTTTTGAAAA ATACTCCAAG GATCTTTTAG TTGAGGGTTT GGCTGAGATA TTGCATCAGA AGTTCATAGA TGCTGACGTG GAGGAACGCC ATCATGCATA CCTGAAGCCT TTTCGCGTCC TCATCAGTCT GCTTGACAAG CCAGAAATAG GGCCTCAAGT GGTTGGGAAT TTGTTTCTCG AAGTCATCAG GGCCTTTTAT TCTTACTGCA GAGATGCCCT TGGCTCTGAT CTTAAACTTA GCTACACCCA GAGTGGAAAT TCGCTGATAA GTGCAATCAA GGAAAACAGA AATGCCTCTG AGATTGTCAA AACGGTAAAT TTGCTGATAA CTTCTCTAAG CACAGACTTT CTCTGGGATT ATATGACAAG GTGTTTTGAG GAATGCTTTA GACCAGTGAA GCAGCGTTAC AGCGTGAGGA ACAGCGTCAG CCCTCCCCCC ACGGTCTCGG AGCTCTGCGC CCTCCTGGTC TTCCTGCTGG ATGTCATTCC TTTGGAACTT TACTCTGAGG TGCAAACCCA GTATCTCCCT CAGGTGCTCG GCTGCCTGGT GCAGCCTCTT GCTGAGGACA TGGAGGCCTT AAGTTTACCT GAACTCACGC ATGCCTTGAA GACGTGTTTC AAGGTGCTCA GCAAAGTCCA GATGCCTCCT TCCTACCTCG ACACGGAGTC CACCAGCGGA ACCTCGAGTC CAGTAAAAGG TGAAAACGGC AAAATAATTT TGGAAACAAA GGCAGTGATT CCCGGTGACG AAGATGCTTC GTTTCCCCCT CTGAAGTCTG AGGACAGTGG GATCGGGCTC AGTGCCTCGT CACCGGAGCT CTCTGAGCAC TTGAGGGTTC CTCGAGTTTC TCTGGAAAGG GACGACGTTT GGAAGAAGGG CGGGAGCATG CAGAGGACGT TTCTTTGCAT CCAAGAGCTA ATCGCCAACT TTGCCAGCAA GAACATTTTT GGAGTACAGC TGACAGCGTC AGGAGAAGAA AGCAAGTCCG AGGAGCCTGC AGGGAAGAGG GACAGGGATG GGACGCAGAG CCTGGCAGCC AATGATTCCA GCAGGAAGAA CTCTTGGGAG CCCAAGCCCA TCACTGTGCC TCAGTTCAAG CAGATGCTGT CAGACTTGTT CACAGCACGA GGGTCTCCAT TCAAGACAAA AAGTTCAGAG TCACCATCGT CTTCGCCCAG CAGCCCTGCC AGGAAAAACG GGGGAGAATG GGATGTTGAG AAGGTGGTCA TTGACCTGGG GGGTTCCAGG GAGGAACGCA GGGAGGCCTT TGCCGCCGCC TGCCACCTGC TGCTGGATTG TGCCACTTTC CCTGTCTACC TGTCCGAGGA AGAGACCGAG CAGCTCTGTG CAACGCTCTT CCAGCTGCCA GGAGCCGGTG ATTCCAGTTT TCCATCTTGG CTGAAGTCCC TCATGACTAT TTGCTGCTGT GTGACTGACT GCTACCTCCA GAACGTGGCC ATTTCCACTC TGCTGGAAGT GATAAACCAT TCCCAGTCCC TGGCGCTTGT CATTGAAGAC AAGATGAAAC GCTATAAGAG CTCTGGACAC AACCCTTTTT TTGGCAAGCT GCAGATGGTG ACGGTTCCTC CCATTGCTCC AGGGATATTG AAAGTCATTG CAGAGAAAAC AGATTTCTAT CAGAGGGTGG CTCGTGTGCT TTGGAATCAG CTGAACAAAG AGACCCGGGA GCATCACGTC ACCTGCGTAG AATTGTTCTA CCGGCTGCAC TGCCTGGCCC CTACGGCCAA CATCTGCGAG GACATCATCT GCCATGCCCT CCTGGACCCT GACAAGGGAA CAAGGCTGGA AGCTCTGTTT AGATTTTCCG TGATCTGGCA TCTGACAAGA GAGATCCAAG GCAGTCGAGT AACATCTCAC AATCGCTCCT TTGATAGGTC CTTGTTTGTC GTGCTGGACA GCCTGGCCTG CACGGATGGT GCCATCGGTG CGGCAGCCCA GGGCTGGCTG GTGCGTGCGC TCTCCCTCGG GGACGTGGCT CGCATCCTCG AACCCGTGCT CCTGCTGCTG CTGCAGCCAA AAACCCAGAG AACCTCCATC CACTGCCTCA AGCAGGAGAA CTCGGCCGAT GACTTGCACC GTTGGTTTAA CAGGAAGAAA ACCTCTTTCA GAGAGGCATG CGCAGTGCCC GAGCCTCAGG AGAGCGGCTC TGAAGAGCAC CTGCCTCTGA GCCAGTTCAC CACAGTGGAC CGTGAAGCCA TTTGGGCCGA AGTGGAGAAG GAGCCCGAGA AGTACCCGCT GCGAGGCGAG CTGAGCGAGG AAGAGCTGCC CTACTACGTG GAGCTTCCAG ACAGGACGGC CCACGGCGCC CCGGACAGCA GCGAGCACAC CGAGTCTGCA GATACAAGCT CCTGCCACAC GGACAGCGAG AACACGTCCT CCTTCTCCTC CCCTTCCCAC GACCTGCAGG AGCTGAGCAA CGAAGAGAAC TGCTGTGCAC CCATCCCCAT GGGGGGCAGG GCGTACCCCA AGCGCTCGGC CCTGCTGGCG GCCTTCCAGT CAGAAAGCTT CAAGGCTGGG GCCAAGTTAA GCCTGGTGCG GGTGGACTCG GACAAGACGC AGGCTTCTGA GTCGTTCTCC AGCGACGAGG AGGCGGACTT GGAGCTCCAG GCCCTCACCA CATCCAGGCT GCTAAAGCAG CAGCGGGAAA GGCAGGAGGC CGTCGAGGCC TTGTTCAAGC ACATCCTGCT CTACCTGCAG CCCTACGACT CTCGGCGGGT CCTCTATGCC TTCTCGGTGC TGGAGGCTGT GCTCAAAACC AACCCTAAGG AATTCATCGA GGCTGTGTCC AGGACTAGCA TGGATACCAG CTCCACCGCG CACCTCAACC TCATCTCCAA CCTCCTCGCT CGCCACCAGG AGGCCCTCAT TGGCCAGAGT TTCTACGGAA AGCTCCAGAC CCAGGTCCCC AACGTGTGCC CCCACTCTCT GCTCCTGGAG CTGCTCACCT ACCTCTGCCT GAGCTTCCTG CGCTCCTACT ACCCTTGCTA TTTGAAGGTC TCGCACCGAG ACATTCTCGG CAACCGGGAC GTGCAGGTCA AAAGTGTCGA GGTTTTGATC AGGATAATGA TGCAGCTGGT CTCAGTGGCC AAGTCTTCGG AAGGGAAGAA CGTGGAGTTC ATCCACAGCT TGCTGCAGAG GTGCAAAGTT CAGGAGTTTG TCCTGCTCTC CCTGTCGGCG TCCATGTACA CGAGCCAGAA GCGCTACGGG CTGGCCACCG CCCACCACGG CAGGGCCCTG CCAGAGGACA GCCTCTTTGA GGAGAGTCTC ATTAACTTGG GTCAGGACCA GATCTGGAGT GAGCACCCGC TGCAGATTGA GCTGCTGAAG CTGCTGCAGG TGCTGATTGT CTTGGAACAC CACCTGGGTC GGGCCCATGA GGAGGCGGAA AACCAGCCCG ACCTGTCCCG GGAGTGGCAG AGAGCCCTGA ACTTCCAGCA GGCCATCAGC GCCCTGCAGT ACGTGCAGCC CCACCCCCTC ACCTCCCAGG GTCTTCTGGT CTCTGCGGTG GTGAGGGGTC TGCAGCCCGC CTACGGTTAC GGCATGCATC CGGCCTGGGT GAGCTTGGTC ACGCATTCCT TGCCCTACTT CGGAAAGTCC CTGGGCTGGA CGGTGACACC CTTTGTTGTC CAGATTTGCA AAAACTTGGA TGACTTGGTC AAGCAGTATG AAAGCGAATC TGTGAAGCTC TCTGTCAGCA CAACCTCCAA GAGGGAAAAC ATTTCTCCAG ATTATCCACT CACCCTTCTA GAAGGTCTAA CGACCATTAG TCATTTTTGT CTTTTGGAAC AAGCCAACCA AAACAAAAAG ACCATGGCTG CAGGTGATCC TGCCAACTTG AGGAATGCCA GAAATGCCAT TTTGGAAGAG CTGCCTCGAA CTGTTAACAC CATGGCCCTT CTCTGGAATG TTCTCAGAAA GGAGGAGACT CAAAAGAGAC CTGTCGATCT CCTAGGGGCC ACGAAGGGAT CCTCTTCCGT TTACTTTAAA ACCACCAAAA CCATAAGACA AAAAATTTTA GACTTCTTAA ACCCCTTGAC GGCCCATCTT GGGGTTCAGT TGACAGCGGC TGTTGCGGCA GTGTGGAGCA GAAAGAAAGC CCAGCGTCAC AGTAAGATGA AGATTATCCC AACGGCAAGT GCATCCCAGC TAACCCTTGT CGACTTGGTG TGTGCACTCA GCACCCTGCA GACTGACACG CTGCTGCACC TGGTGAAGGA GGTGGTGAAG AGGCCACCCC AAGTCAAAGG GGGTGATGAG AAATCGCCCC TAGTGGACAT TCCTGTGTTG CAGTTTTGCT ATGCTTTTCT CCAAAGGCTC CCAGTACCAG CCTTGCAAGA GAACTTTTCT TCACTGTTGG GAGTATTGAA AGAGTCTGTA CAGTTGAATC TAGCCCCACC TGGGTATTTT CTGCTTCTCA GCATGCTGAA TGACTTTGTA ACAAGAACTC CCAACCTGGA AAACAAGAAG GACCAAAAAG ACCTGCAGGA AATCACTCAG AAAATCCTAG AAGCTGTGGG GAACATTGCC GGCTCTTCCT TGGAGCAAAC CAGCTGGCTA AGCAGAAACC TGGAAGTGAA GGCCCAACCT CAGGCCTCTC TAGAAGAATC TGATGCTGAG GAGGACCTGT ATGATGCTGC TGCAGCTTCA GCAATGGTGT CTTCATCCGC CCCGTCGGTG TACAGCGTGC AAGCCCTCTC TCTCCTGGCA GAGGTACTGG CTTCCCTCCT GGACATGGTT TATCGAAGTG ATGAGAAGGA GAAAGCTGTG CCGTTAATCT CCCGTCTGCT TTACTATGTT TTTCCATACT TACGCAACCA CAGTGCCTAC AATGCTCCCA GCTTCCGGGC TGGCGCTCAG CTGCTGAGCT CCCTGAGTGG CTATGCCTAC ACAAAGCGAG CCTGGAGGAA GGAGGTCCTG GAGCTGTTTC TCGACCCCGC TTTCTTTCAG ATGGATACTT CCTGTGTTCA TTGGAAGTCC ATTATTGACC ATCTTTTGAC TCATGAGAAA ACAATGTTTA AGGATTTAAT GAACATGCAG AGCAGTTCTT TGAAACTATT CTCAAGTTTT GAACAGAAAG CCATGCTGTT AAAGCGCCAG GCTTTTGCTG TCTTCAGTGG AGAACTTGAT CAATACCACC TTTACCTTCC ACTGATACAA GAACGCCTGA CAGACAATCT CAGAGTTGGA CAGACATCCA TAGTTGCTGC TCAGATGTTT CTTTTTTTCA GAGTTTTGCT GCTAAGAATA TCTCCTCAAC ATTTGACTTC ATTGTGGCCA ATAATGGTCT CTGAATTGAT TCAGACATTC ACACAGCTTG AAGAAGATCT AAAAGATGAA GATGAGTCAT TGAGAAGCAC CAACAAAGTA AACAGAACGA AAGTTTCAGT CCCGGATGCA AATGGACCCT CAGTGGGGGA GATACCCCAG AGTGAACTCA TCTTGTATTT ATCAGCTTGC AAATTCTTGG ACACAGCGCT TTCTTTTCCA CCTGACAAGA TGCCATTATT TCAAATTTAT AGGTGGGCAT TTATTCCAGA AGTGGACACA GAGGGCCCTG CCTTCCTGTC GGATGTAGAG GAGAATCACC AAGAATGCAA ACCCCACACT GTCAGGATTC TAGAACTTCT AAAATTAAAG TTTGGGGAAA TCAGTAGCTC TGATGAGATC ACCATGAAGA GTGAATTCCC GCTTCTGCGC CAACATTCTG TTTCCAGCAT CAGGCAGTTG ATGCCATTCT TCATGACTCT AAATGGTGCA TTTAAGACCC AGAGACAGCT GCCTGCTGAT AGCCCAGGAA CTCCATTCTT GGACTTTCCT GTCACAGATA GCCCAAGGAT CTTAAAACAA CTGGAAGAAT GCATCGAATA TGATTTTCTG GAACATCCAG AATGTTCAGG CCTCATGGGC CCAGCTTTCT TGTACAAAGT TGGCATTATA AAAAATAATT GCTCATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GCCTCCTCTA GACCAGCCAG GACAGAAATG CCTCGACTTC GCTGCTGCCC AAGGTTGCCG GGTGACGCAC ACCGTGGAAA CGGATGAAGG CACGAACCCA GTGGACATAA GCCTGTTCGG TTCGTAAGCT GTAATGCAAG TAGCGTATGC GCTCACGCAA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG GGGTACAGTC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGGGCAG TCGCCCTAAA ACAAAGTTAA ACATCATGAG GGAAGCGGTG ATCGCCGAAG TATCGACTCA ACTATCAGAG GTAGTTGGCG TCATCGAGCG CCATCTCGAA CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG ATGGCGGCCT GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT TCCCCTGGAG AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT GCACGACGAC ATCATTCCGT GGCGTTATCC AGCTAAGCGC GAACTGCAAT TTGGAGAATG GCAGCGCAAT GACATTCTTG CAGGTATCTT CGAGCCAGCC ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA GAGAACATAG CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG CCGCCCGACT GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG CATTTGGTAC AGCGCAGTAA CCGGCAAAAT CGCGCCGAAG GATGTCGCTG CCGACTGGGC AATGGAGCGC CTGCCGGCCC AGTATCAGCC CGTCATACTT GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT TGGCCTCGCG CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA AGGTAGTCGG CAAATAACCC TCGAGCCACC CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAA
