NM_000876.4(1point mutation1synonymous mutations );CD222; CI-M6PR; CIMPR; M6P-R; M6P/IGF2R;MPR 300; MPR1; MPR300; MPRI
100μg liquid plasmid(Instantly):$178
| name | pENTR223.1-IGF2R(human)(1point mutation1synonymous mutations) | another name | apENTR223.1-IGF2R(human)(1point mutation1synonymous mutations) |
| Insert Size (bp) | 7474bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGGGGGCC GCCGCCGGCC GGAGCCCCCA CCTGGGGCCC GCGCCCGCCC GCCGCCCGCA GCGCTCTCTG CTCCTGCTGC AGCTGCTGCT GCTCGTCGCT GCCCCGGGGT CCACGCAGGC CCAGGCCGCC CCGTTCCCCG AGCTGTGCAG TTATACATGG GAAGCTGTTG ATACCAAAAA TAATGTACTT TATAAAATCA ACATCTGTGG AAGTGTGGAT ATTGTCCAGT GCGGGCCATC AAGTGCTGTT TGTATGCACG ACTTGAAGAC ACGCACTTAT CATTCAGTGG GTGACTCTGT TTTGAGAAGT GCAACCAGAT CTCTCCTGGA ATTCAACACA ACAGTGAGCT GTGACCAGCA AGGCACAAAT CACAGAGTCC AGAGCAGCAT TGCCTTCCTG TGTGGGAAAA CCCTGGGAAC TCCTGAATTT GTAACTGCAA CAGAATGTGT GCACTACTTT GAGTGGAGGA CCACTGCAGC CTGCAAGAAA GACATATTTA AAGCAAATAA GGAGGTGCCA TGCTATGTGT TTGATGAAGA GTTGAGGAAG CATGATCTCA ATCCTCTGAT CAAGCTTAGT GGTGCCTACT TGGTGGATGA CTCCGATCCG GACACTTCTC TATTCATCAA TGTTTGTAGA GACATAGACA CACTACGAGA CCCAGGTTCA CAGCTGCGGG CCTGTCCCCC CGGCACTGCC GCCTGCCTGG TAAGAGGACA CCAGGCGTTT GATGTTGGCC AGCCCCGGGA CGGACTGAAG CTGGTGCGCA AGGACAGGCT TGTCCTGAGT TACGTGAGGG AAGAGGCAGG AAAGCTAGAC TTTTGTGATG GTCACAGCCC TGCGGTGACT ATTACATTTG TTTGCCCGTC GGAGCGGAGA GAGGGCACCA TTCCCAAACT CACAGCTAAA TCCAACTGCC GCTATGAAAT TGAGTGGATT ACTGAGTATG CCTGCCACAG AGATTACCTG GAAAGTAAAA CTTGTTCTCT GAGCGGCGAG CAGCAGGATG TCTCCATAGA CCTCACACCA CTTGCCCAGA GCGGAGGTTC ATCCTATATT TCAGATGGAA AAGAATATTT GTTTTATTTG AATGTCTGTG GAGAAACTGA AATACAGTTC TGTAATAAAA AACAAGCTGC AGTTTGCCAA GTGAAAAAGA GCGATACCTC TCAAGTCAAA GCAGCAGGAA GATACCACAA TCAGACCCTC CGATATTCGG ATGGAGACCT CACCTTGATA TATTTTGGAG GTGATGAATG CAGCTCAGGG TTTCAGCGGA TGAGCGTCAT AAACTTTGAG TGCAATAAAA CCGCAGGTAA CGATGGGAAA GGAACTCCTG TATTCACAGG GGAGGTTGAC TGCACCTACT TCTTCACATG GGACACGGAA TACGCCTGTG TTAAGGAGAA GGAAGACCTC CTCTGCGGTG CCACCGACGG GAAGAAGCGC TATGACCTGT CCGCGCTGGT CCGCCATGCA GAACCAGAGC AGAATTGGGA AGCTGTGGAT GGCAGTCAGA CGGAAACAGA GAAGAAGCAT TTTTTCATTA ATATTTGTCA CAGAGTGCTG CAGGAAGGCA AGGCACGAGG GTGTCCCGAG GACGCGGCAG TGTGTGCAGT GGATAAAAAT GGAAGTAAAA ATCTGGGAAA ATTTATTTCC TCTCCCATGA AAGAGAAAGG AAACATTCAA CTCTCTTATT CAGATGGTGA TGATTGTGGT CATGGCAAGA AAATTAAAAC TAATATCACA CTTGTATGCA AGCCAGGTGA TCTGGAAAGT GCACCAGTGT TGAGAACTTC TGGGGAAGGC GGTTGCTTTT ATGAGTTTGA GTGGCACACA GCTGCGGCCT GTGTGCTGTC TAAGACAGAA GGGGAGAACT GCACGGTCTT TGACTCCCAG GCAGGGTTTT CTTTTGACTT ATCACCTCTC ACAAAGAAAA ATGGTGCCTA TAAAGTTGAG ACAAAGAAGT ATGACTTTTA TATAAATGTG TGTGGCCCGG TGTCTGTGAG CCCCTGTCAG CCAGACTCAG GAGCCTGCCA GGTGGCAAAA AGTGATGAGA AGACTTGGAA CTTGGGTCTG AGTAATGCGA AGCTTTCATA TTATGATGGG ATGATCCAAC TGAACTACAG AGGCGGCACA CCCTATAACA ATGAAAGACA CACACCGAGA GCTACGCTCA TCACCTTTCT CTGTGATCGA GACGCGGGAG TGGGCTTCCC TGAATATCAG GAAGAGGATA ACTCCACCTA CAACTTCCGG TGGTACACCA GCTATGCCTG CCCGGAGGAG CCCCTGGAAT GCGTAGTGAC CGACCCCTCC ACGCTGGAGC AGTACGACCT CTCCAGTCTG GCAAAATCTG AAGGTGGCCT TGGAGGAAAC TGGTATGCCA TGGACAACTC AGGGGAACAT GTCACGTGGA GGAAATACTA CATTAACGTG TGTCGGCCTC TGAATCCAGT GCCGGGCTGC AACCGATATG CATCGGCTTG CCAGATGAAG TATGAAAAAG ATCAGGGCTC CTTCACTGAA GTGGTTTCCA TCAGTAACTT GGGAATGGCA AAGACCGGCC CGGTGGTTGA GGACAGCGGC AGCCTCCTTC TGGAATACGT GAATGGGTCG GCCTGCACCA CCAGCGATGG CAGACAGACC ACATATACCA CGAGGATCCA TCTCGTCTGC TCCAGGGGCA GGCTGAACAG CCACCCCATC TTTTCTCTCA ACTGGGAGTG TGTGGTCAGT TTCCTGTGGA ACACAGAGGC TGCCTGTCCC ATTCAGACAA CGACGGATAC AGACCAGGCT TGCTCTATAA GGGATCCCAA CAGTGGATTT GTGTTTAATC TTAATCCGCT AAACAGTTCG CAAGGATATA ACGTCTCTGG CATTGGGAAG ATTTTTATGT TTAATGTCTG CGGCACAATG CCTGTCTGTG GGACCATCCT GGGAAAACCT GCTTCTGGCT GTGAGGCAGA AACCCAAACT GAAGAGCTCA AGAATTGGAA GCCAGCAAGG CCAGTCGGAA TTGAGAAAAG CCTCCAGCTG TCCACAGAGG GCTTCATCAC TCTGACCTAC AAAGGGCCTC TCTCTGCCAA AGGTACCGCT GATGCTTTTA TCGTCCGCTT TGTTTGCAAT GATGATGTTT ACTCAGGGCC CCTCAAATTC CTGCATCAAG ATATCGACTC TGGGCAAGGG ATCCGAAACA CTTACTTTGA GTTTGAAACC GCGTTGGCCT GTGTTCCTTC TCCAGTGGAC TGCCAAGTCA CCGACCTGGC TGGAAATGAG TACGACCTGA CTGGCCTAAG CACAGTCAGG AAACCTTGGA CGGCTGTTGA CACCTCTGTC GATGGGAGAA AGAGGACTTT CTATTTGAGC GTTTGCAATC CTCTCCCTTA CATTCCTGGA TGCCAGGGCA GCGCAGTGGG GTCTTGCTTA GTGTCAGAAG GCAATAGCTG GAATCTGGGT GTGGTGCAGA TGAGTCCCCA AGCCGCGGCG AATGGATCTT TGAGCATCAT GTATGTCAAC GGTGACAAGT GTGGGAACCA GCGCTTCTCC ACCAGGATCA CGTTTGAGTG TGCTCAGATA TCGGGCTCAC CAGCATTTCA GCTTCAGGAT GGTTGTGAGT ACGTGTTTAT CTGGAGAACT GTGGAAGCCT GTCCCGTTGT CAGAGTGGAA GGGGACAACT GTGAGGTGAA AGACCCAAGG CATGGCAACT TGTATGACCT GAAGCCCCTG GGCCTCAACG ACACCATCGT GAGCGCTGGC GAATACACTT ATTACTTCCG GGTCTGTGGG AAGCTTTCCT CAGACGTCTG CCCCACAAGT GACAAGTCCA AGGTGGTCTC CTCATGTCAG GAAAAGCGGG AACCGCAGGG ATTTCACAAA GTGGCAGGTC TCCTGACTCA GAAGCTAACT TATGAAAATG GCTTGTTAAA AATGAACTTC ACGGGGGGGG ACACTTGCCA TAAGGTTTAT CAGCGCTCCA CAGCCATCTT CTTCTACTGT GACCGCGGCA CCCAGCGGCC AGTATTTCTA AAGGAGACTT CAGATTGTTC CTACTTGTTT GAGTGGCGAA CGCAGTATGC CTGCCCACCT TTCGATCTGA CTGAATGTTC ATTCAAAGAT GGGGCTGGCA ACTCCTTCGA CCTCTCGTCC CTGTCAAGGT ACAGTGACAA CTGGGAAGCC ATCACTGGGA CGGGGGACCC GGAGCACTAC CTCATCAATG TCTGCAAGTC TCTGGCCCCG CAGGCTGGCA CTGAGCCGTG CCCTCCAGAA GCAGCCGCGT GTCTGCTGGG TGGCTCCAAG CCCGTGAACC TCGGCAGGGT AAGGGACGGA CCTCAGTGGA GAGATGGCAT AATTGTCCTG AAATACGTTG ATGGCGACTT ATGTCCAGAT GGGATTCGGA AAAAGTCAAC CACCATCCGA TTCACCTGCA GCGAGAGCCA AGTGAACTCC AGGCCCATGT TCATCAGCGC CGTGGAGGAC TGTGAGTACA CCTTTGCCTG GCCCACAGCC ACAGCCTGTC CCATGAAGAG CAACGAGCAT GATGACTGCC AGGTCACCAA CCCAAGCACA GGACACCTGT TTGATCTGAG CTCCTTAAGT GGCAGGGCGG GATTCACAGC TGCTTACAGC GAGAAGGGGT TGGTTTACAT GAGCATCTGT GGGGAGAATG AAAACTGCCC TCCTGGCGTG GGGGCCTGCT TTGGACAGAC CAGGATTAGC GTGGGCAAGG CCAACAAGAG GCTGAGATAC GTGGACCAGG TCCTGCAGCT GGTGTACAAG GATGGGTCCC CTTGTCCCTC CAAATCCGGC CTGAGCTATA AGAGTGTGAT CAGTTTCGTG TGCAGGCCTG AGGCCAGGCC AACCAATAGG CCCATGCTCA TCTCCCTGGA CAAGCAGACA TGCACTCTCT TCTTCTCCTG GCACACGCCG CTGGCCTGCG AGCAAGCGAC CGAATGTTCC GTGAGGAATG GAAGCTCTAT TGTTGACTTG TCTCCCCTTA TTCATCGCAC TGGTGGTTAT GAGGCTTATG ATGAGAGTGA GGATGATGCC TCCGATACCA ACCCTGATTT CTACATCAAT ATTTGTCAGC CACTAAATCC CATGCACGGA GTGCCCTGTC CTGCCGGAGC CGCTGTGTGC AAAGTTCCTA TTGATGGTCC CCCCATAGAT ATCGGCCGGG TAGCAGGACC ACCAATACTC AATCCAATAG CAAATGAGAT TTACTTGAAT TTTGAAAGCA GTACTCCTTG CTTAGCGGAC AAGCATTTCA ACTACACCTC GCTCATCGCG TTTCACTGTA AGAGAGGTGT GAGCATGGGA ACGCCTAAGC TGTTAAGGAC CAGCGAGTGC GACTTTGTGT TCGAATGGGA GACTCCTGTC GTCTGTCCTG ATGAAGTGAG GATGGATGGC TGTACCCTGA CAGATGAGCA GCTCCTCTAC AGCTTCAACT TGTCCAGCCT TTCCACGAGC ACCTTTAAGG TGACTCGCGA CTCGCGCACC TACAGCGTTG GGGTGTGCAC CTTTGCAGTC GGGCCAGAAC AAGGAGGCTG TAAGGACGGA GGAGTCTGTC TGCTCTCAGG CACCAAGGGG GCATCCTTTG GACGGCTGCA ATCAATGAAA CTGGATTACA GGCACCAGGA TGAAGCGGTC GTTTTAAGTT ACGTGAATGG TGATCGTTGC CCTCCAGAAA CCGATGACGG CGTCCCCTGT GTCTTCCCCT TCATATTCAA TGGGAAGAGC TACGAGGAGT GCATCATAGA GAGCAGGGCG AAGCTGTGGT GTAGCACAAC TGCGGACTAC GACAGAGACC ACGAGTGGGG CTTCTGCAGA CACTCAAACA GCTACCGGAC ATCCAGCATC ATATTTAAGT GTGATGAAGA TGAGGACATT GGGAGGCCAC AAGTCTTCAG TGAAGTGCGT GGGTGTGATG TGACATTTGA GTGGAAAACA AAAGTTGTCT GCCCTCCAAA GAAGTTGGAG TGCAAATTCG TCCAGAAACA CAAAACCTAC GACCTGCGGC TGCTCTCCTC TCTCACCGGG TCCTGGTCCC TCGTCCACAA CGGAGTCTCG TACTATATAA ATCTGTGCCA GAAAATATAT AAAGGGCCCC TGGGCTGCTC TGAAAGGGCC AGCATTTGCA GAAGGACCAC AACTGGTGAC GTCCAGGTCC TGGGACTCGT TCACACGCAG AAGCTGGGTG TCATAGGTGA CAAAGTTGTT GTCACGTACT CCAAAGGTTA TCCGTGTGGT GGAAATAAGA CCGCATCCTC CGTGATAGAA TTGACCTGTA CAAAGACGGT GGGCAGACCT GCATTCAAGA GGTTTGATAT CGACAGCTGC ACTTACTACT TCAGCTGGGA CTCCCGGGCT GCCTGCGCCG TGAAGCCTCA GGAGGTGCAG ATGGTGAATG GGACCATCAC CAACCCTATA AATGGCAAGA GCTTCAGCCT CGGAGATATT TATTTTAAGC TGTTCAGAGC CTCTGGGGAC ATGAGGACCA ATGGGGACAA CTACCTGTAT GAGATCCAAC TTTCCTCCAT CACAAGCTCC AGAAACCCGG CGTGCTCTGG AGCCAACATA TGCCAGGTGA AGCCCAACGA TCAGCACTTC AGTCGGAAAG TTGGAACCTC TGACAAGACC AAGTACTACC TTCAAGACGG CGATCTCGAT GTCGTGTTTG CCTCTTCCTC TAAGTGCGGA AAGGATAAGA CCAAGTCTGT TTCTTCCACC ATCTTCTTCC ACTGTGACCC TCTGGTGGAG GACGGGATCC CCGAGTTCAG TCACGAGACT GCCGACTGCC AGTACCTCTT CTCTTGGTAC ACCTCAGCCG TGTGTCCTCT GGGGGTGGGC TTTGACAGCG AGAATCCCGG GGACGACGGG CAGATGCACA AGGGGCTGTC AGAACGGAGC CAGGCAGTCG GCGCGGTGCT CAGCCTGCTG CTGGTGGCGC TCACCTGCTG CCTGCTGGCC CTGTTGCTCT ACAAGAAGGA GAGGAGGGAA ACAGTGATAA GTAAGCTGAC CACTTGCTGT AGGAGAAGTT CCAACGTGTC CTACAAATAC TCAAAGGTGA ATAAGGAAGA AGAGACAGAT GAGAATGAAA CAGAGTGGCT GATGGAAGAG ATCCAGCTGC CTCCTCCACG GCAGGGAAAG GAAGGGCAGG AGAACGGCCA TATTACCACC AAGTCAGTGA AAGCCCTCAG CTCCCTGCAT GGGGATGACC AGGACAGTGA GGATGAGGTT CTGACCATCC CAGAGGTGAA AGTTCACTCG GGCAGGGGAG CTGGGGCAGA GAGCTCCCAC CCAGTGAGAA ACGCACAGAG CAATGCCCTT CAGGAGCGTG AGGACGATAG GGTGGGGCTG GTCAGGGGTG AGAAGGCGAG GAAAGGGAAG TCCAGCTCTG CACAGCAGAA GACAGTGAGC TCCACCAAGC TGGTGTCCTT CCATGACGAC AGCGACGAGG ACCTCTTACA CATCTCAGGC CTCATGGGCC CAGCTTTCTT GTACAAAGTT GGCATTATAA AAAATAATTG CTCATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG CCTCCTCTAG ACCAGCCAGG ACAGAAATGC CTCGACTTCG CTGCTGCCCA AGGTTGCCGG GTGACGCACA CCGTGGAAAC GGATGAAGGC ACGAACCCAG TGGACATAAG CCTGTTCGGT TCGTAAGCTG TAATGCAAGT AGCGTATGCG CTCACGCAAC TGGTCCAGAA CCTTGACCGA ACGCAGCGGT GGTAACGGCG CAGTGGCGGT TTTCATGGCT TGTTATGACT GTTTTTTTGG GGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA CATCATGAGG GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT CATCGAGCGC CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA TGGCGGCCTG AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA TGAAACAACG CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA GAGCGAGATT CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG GCGTTATCCA GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC AGGTATCTTC GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG AGAACATAGC GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA ACAGGATCTA TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG GGCTGGCGAT GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC CGGCAAAATC GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA GTATCAGCCC GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT GGCCTCGCGC GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA GGTAGTCGGC AAATAACCCT CGAGCCACCC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AA
