pTNT-cirRNA
$113.00
In stock
SKU
PL46668
100μg liquid plasmid(Instantly):$134
| name | pTNT-cirRNA | another name | apTNT-cirRNA |
| Insert Size (bp) | 1763bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene cloning | Fragment type | cDNA |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7,SP6 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCA TTTAGGTGAC ACTATAGAGG GAGACCCTCG ACCGTCGATT GTCCACTGGT CAACAATAGA TGACTTACAA CTAATCGGAA GGTGCAGAGA CTCGACGGGA GCTACCCTAA CGTCAAGACG AGGGTAAAGA GAGAGTCCAA TTCTCAAAGC CAATAGGCAG TAGCGAAAGC TGCAAGAGAA TGAAAATCCG TTGACCTTAA ACGGTCGTGT GGGTTCAAGT CCCTCCACCC CCACGCCGGA AACGCAATAG CCGAAAAACA AAAAACAAAA AAAACAAAAA AAAAACCAAA AAAACAAAAC ACATTAAAAC AGCCTGTGGG TTGATCCCAC CCACAGGCCC ATTGGGCGCT AGCACTCTGG TATCACGGTA CCTTTGTGCG CCTGTTTTAT ACCCCCTCCC CCAACTGTAA CTTAGAAGTA ACACACACCG ATCAACAGTC AGCGTGGCAC ACCAGCCACG TTTTGATCAA GCACTTCTGT TACCCCGGAC TGAGTATCAA TAGACTGCTC ACGCGGTTGA AGGAGAAAGC GTTCGTTATC CGGCCAACTA CTTCGAAAAA CCTAGTAACA CCGTGGAAGT TGCAGAGTGT TTCGCTCAGC ACTACCCCAG TGTAGATCAG GTCGATGAGT CACCGCATTC CCCACGGGCG ACCGTGGCGG TGGCTGCGTT GGCGGCCTGC CCATGGGGAA ACCCATGGGA CGCTCTAATA CAGACATGGT GCGAAGAGTC TATTGAGCTA GTTGGTAGTC CTCCGGCCCC TGAATGCGGC TAATCCTAAC TGCGGAGCAC ACACCCTCAA GCCAGAGGGC AGTGTGTCGT AACGGGCAAC TCTGCAGCGG AACCGACTAC TTTGGGTGTC CGTGTTTCAT TTTATTCCTA TACTGGCTGC TTATGGTGAC AATTGAGAGA TCGTTACCAT ATAGCTATTG GATTGGCCAT CCGGTGACTA ATAGAGCTAT TATATATCCC TTTGTTGGGT TTATACCACT TAGCTTGAAA GAGGTTAAAA CATTACAATT CATTGTTAAG TTGAATACAG CAAAGCCACC ATGGGAGTCA AAGTTCTGTT TGCCCTGATC TGCATCGCTG TGGCCGAGGC CAAGCCCACC GAGAACAACG AAGACTTCAA CATCGTGGCC GTGGCCAGCA ACTTCGCGAC CACGGATCTC GATGCTGACC GCGGGAAGTT GCCCGGCAAG AAGCTGCCGC TGGAGGTGCT CAAAGAGATG GAAGCCAATG CCCGGAAAGC TGGCTGCACC AGGGGCTGTC TGATCTGCCT GTCCCACATC AAGTGCACGC CCAAGATGAA GAAGTTCATC CCAGGACGCT GCCACACCTA CGAAGGCGAC AAAGAGTCCG CACAGGGCGG CATAGGCGAG GCGATCGTCG ACATTCCTGA GATTCCTGGG TTCAAGGACT TGGAGCCCAT GGAGCAGTTC ATCGCACAGG TCGATCTGTG TGTGGACTGC ACAACTGGCT GCCTCAAAGG GCTTGCCAAC GTGCAGTGTT CTGACCTGCT CAAGAAGTGG CTGCCGCAAC GCTGTGCGAC CTTTGCCAGC AAGATCCAGG GCCAGGTGGA CAAGATCAAG GGGGCCGGTG GTGACTAAAA AAAACAAAAA ACAAAACGGC TATTATGCGT TACCGGCGAG ACGCTACGGA CTTAAATAAT TGAGCCTTAA AGAAGAAATT CTTTAAGTGG ATGCTCTCAA ACTCAGGGAA ACCTAAATCT AGTTATAGAC AAGGCAATCC TGAGCCAAGC CGAAGTAGTA ATTAGTAAGA CCAGTGGACA ATCGACGGAT AACAGCATAT CTAGAAAAAA AAAAAAAAAA AAAAAAAAAA AAAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGGATCCGGG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCT
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