NM_004385.5(2synonymous mutations );CSPG2; ERVR; GHAP; PG-M; WGN; WGN1
100μg liquid plasmid(Instantly):$221
| name | pENTR223.1-VCAN(human)(2synonymous mutations) | another name | apENTR223.1-VCAN(human)(2synonymous mutations) |
| Insert Size (bp) | 10189bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGTTCATA AATATAAAGA GCATCTTATG GATGTGTTCA ACCTTAATAG TAACCCATGC GCTACATAAA GTCAAAGTGG GAAAAAGCCC ACCGGTGAGG GGCTCCCTCT CTGGAAAAGT CAGCCTACCT TGTCATTTTT CAACGATGCC TACTTTGCCA CCCAGTTACA ACACCAGTGA ATTTCTCCGC ATCAAATGGT CTAAGATTGA AGTGGACAAA AATGGAAAAG ATTTGAAAGA GACTACTGTC CTTGTGGCCC AAAATGGAAA TATCAAGATT GGTCAGGACT ACAAAGGGAG AGTGTCTGTG CCCACACATC CCGAGGCTGT GGGCGATGCC TCCCTCACTG TGGTCAAGCT GCTGGCAAGT GATGCGGGTC TTTACCGCTG TGACGTCATG TACGGGATTG AAGACACACA AGACACGGTG TCACTGACTG TGGATGGGGT TGTGTTTCAC TACAGGGCGG CAACCAGCAG GTACACACTG AATTTTGAGG CTGCTCAGAA GGCTTGTTTG GACGTTGGGG CAGTCATAGC AACTCCAGAG CAGCTCTTTG CTGCCTATGA AGATGGATTT GAGCAGTGTG ACGCAGGCTG GCTGGCTGAT CAGACTGTCA GATATCCCAT CCGGGCTCCC AGAGTAGGCT GTTATGGAGA TAAGATGGGA AAGGCAGGAG TCAGGACTTA TGGATTCCGT TCTCCCCAGG AAACTTACGA TGTGTATTGT TATGTGGATC ATCTGGATGG TGATGTGTTC CACCTCACTG TCCCCAGTAA ATTCACCTTC GAGGAGGCTG CAAAAGAGTG TGAAAACCAG GATGCCAGGC TGGCAACAGT GGGGGAACTC CAGGCGGCAT GGAGGAACGG CTTTGACCAG TGCGATTACG GGTGGCTGTC GGATGCCAGC GTGCGCCACC CTGTGACTGT GGCCAGGGCC CAGTGTGGAG GTGGTCTACT TGGGGTGAGA ACCCTGTATC GTTTTGAGAA CCAGACAGGC TTCCCTCCCC CTGATAGCAG ATTTGATGCC TACTGCTTTA AACCTAAAGA GGCTACAACC ATCGATTTGA GTATCCTCGC AGAAACTGCA TCACCCAGTT TATCCAAAGA ACCACAAATG GTTTCTGATA GAACTACACC AATCATCCCT TTAGTTGATG AATTACCTGT CATTCCAACA GAGTTCCCTC CCGTGGGAAA TATTGTCAGT TTTGAACAGA AAGCCACAGT CCAACCTCAG GCTATCACAG ATAGTTTAGC CACCAAATTA CCCACACCTA CTGGCAGTAC CAAGAAGCCC TGGGATATGG ATGACTACTC ACCTTCTGCT TCAGGACCTC TTGGAAAGCT AGACATATCA GAAATTAAGG AAGAAGTGCT CCAGAGTACA ACTGGCGTCT CTCATTATGC TACGGATTCA TGGGATGGTG TCGTGGAAGA TAAACAAACA CAAGAATCGG TTACACAGAT TGAACAAATA GAAGTGGGTC CTTTGGTAAC ATCTATGGAA ATCTTAAAGC ACATTCCTTC CAAGGAATTC CCTGTAACTG AAACACCATT GGTAACTGCA AGAATGATCC TGGAATCCAA AACTGAAAAG AAAATGGTAA GCACTGTTTC TGAATTGGTA ACCACAGGTC ACTATGGATT CACCTTGGGA GAAGAGGATG ATGAAGACAG AACACTTACA GTTGGATCTG ATGAGAGCAC CTTGATCTTT GACCAAATTC CTGAAGTCAT TACGGTGTCA AAGACTTCAG AAGACACCAT CCACACTCAT TTAGAAGACT TGGAGTCAGT CTCAGCATCC ACAACTGTTT CCCCTTTAAT TATGCCTGAT AATAATGGAT CATCCATGGA TGACTGGGAA GAGAGACAAA CTAGTGGTAG GATAACGGAA GAGTTTCTTG GCAAATATCT GTCTACTACA CCTTTTCCAT CACAGCATCG TACAGAAATA GAATTGTTTC CTTATTCTGG TGATAAAATA TTAGTAGAGG GAATTTCCAC AGTTATTTAT CCTTCTCTAC AAACAGAAAT GACACATAGA AGAGAAAGAA CAGAAACACT AATACCAGAG ATGAGAACAG ATACTTATAC AGATGAAATA CAAGAAGAGA TCACTAAAAG TCCATTTATG GGAAAAACAG AAGAAGAAGT CTTCTCTGGG ATGAAACTCT CTACATCTCT CTCAGAGCCA ATTCATGTTA CAGAGTCTTC TGTGGAAATG ACCAAGTCTT TTGATTTCCC AACATTGATA ACAAAGTTAA GTGCAGAGCC AACAGAAGTA AGAGATATGG AGGAAGACTT TACAGCAACT CCAGGTACTA CAAAATATGA TGAAAATATT ACAACAGTGC TTTTGGCCCA TGGTACTTTA AGTGTTGAAG CAGCCACTGT ATCAAAATGG TCATGGGATG AAGATAATAC AACATCCAAG CCTTTAGAGT CTACAGAACC TTCAGCCTCT TCAAAATTGC CCCCTGCCTT ACTCACAACT GTGGGGATGA ATGGAAAGGA TAAAGACATC CCAAGTTTCA CTGAAGATGG AGCAGATGAA TTTACTCTTA TTCCAGATAG TACTCAAAAG CAGTTAGAGG AGGTTACTGA TGAAGACATA GCAGCCCATG GAAAATTCAC AATTAGATTT CAGCCAACTA CATCAACTGG TATTGCAGAA AAGTCAACTT TGAGAGATTC TACAACTGAA GAAAAAGTTC CACCTATCAC AAGCACTGAA GGCCAAGTTT ATGCAACCAT GGAAGGAAGT GCTTTGGGTG AAGTAGAAGA TGTGGACCTC TCTAAGCCAG TATCTACTGT TCCCCAATTT GCACACACTT CAGAGGTGGA AGGATTAGCA TTTGTTAGTT ATAGTAGCAC CCAAGAGCCT ACTACTTATG TAGACTCTTC CCATACCATT CCTCTTTCTG TAATTCCCAA GACAGACTGG GGAGTGTTAG TACCTTCTGT TCCATCAGAA GATGAAGTTC TAGGTGAACC CTCTCAAGAC ATACTTGTCA TTGATCAGAC TCGCCTTGAA GCGACTATTT CTCCAGAAAC TATGAGAACA ACAAAAATCA CAGAGGGAAC AACTCAGGAA GAATTCCCTT GGAAAGAACA GACTGCAGAG AAACCAGTTC CTGCTCTCAG TTCTACAGCT TGGACTCCCA AGGAGGCAGT AACACCACTG GATGAACAAG AGGGCGATGG ATCAGCATAT ACAGTCTCTG AAGATGAATT GTTGACAGGT TCTGAGAGGG TCCCAGTTTT AGAAACAACT CCAGTTGGAA AAATTGATCA CAGTGTGTCT TATCCACCAG GTGCTGTAAC TGAGCACAAA GTGAAAACAG ATGAAGTGGT AACACTAACA CCACGCATTG GGCCAAAAGT ATCTTTAAGT CCAGGGCCTG AACAAAAATA TGAAACAGAA GGTAGTAGTA CAACAGGATT TACATCATCT TTGAGTCCTT TTAGTACCCA CATTACCCAG CTTATGGAAG AAACCACTAC TGAGAAAACA TCCCTAGAGG ATATTGATTT AGGCTCAGGA TTATTTGAAA AGCCCAAAGC CACAGAACTC ATAGAATTTT CAACAATCAA AGTCACAGTT CCAAGTGATA TTACCACTGC CTTCAGTTCA GTAGACAGAC TTCACACAAC TTCAGCATTC AAGCCATCTT CCGCGATCAC TAAGAAACCA CCTCTCATCG ACAGGGAACC TGGTGAAGAA ACAACCAGTG ACATGGTAAT CATTGGAGAA TCAACATCTC ATGTTCCTCC CACTACCCTT GAAGATATTG TAGCCAAGGA AACAGAAACC GATATTGATA GAGAGTATTT CACGACTTCA AGTCCTCCTG CTACACAGCC AACAAGACCA CCCACTGTGG AAGACAAAGA GGCCTTTGGA CCTCAGGCGC TTTCTACGCC ACAGCCCCCA GCAAGCACAA AATTTCACCC TGACATTAAT GTTTATATTA TTGAGGTCAG AGAAAATAAG ACAGGTCGAA TGAGTGATTT GAGTGTAATT GGTCATCCAA TAGATTCAGA ATCTAAAGAA GATGAACCTT GTAGTGAAGA AACAGATCCA GTGCATGATC TAATGGCTGA AATTTTACCT GAATTCCCTG ACATAATTGA AATAGACCTA TACCACAGTG AAGAAAATGA AGAAGAAGAA GAAGAGTGTG CAAATGCTAC TGATGTGACA ACCACCCCAT CTGTGCAGTA CATAAATGGG AAGCATCTCG TTACCACTGT GCCCAAGGAC CCAGAAGCTG CAGAAGCTAG GCGTGGCCAG TTTGAAAGTG TTGCACCTTC TCAGAATTTC TCGGACAGCT CTGAAAGTGA TACTCATCCA TTTGTAATAG CCAAAACGGA ATTGTCTACT GCTGTGCAAC CTAATGAATC TACAGAAACA ACTGAGTCTC TTGAAGTTAC ATGGAAGCCT GAGACTTACC CTGAAACATC AGAACATTTT TCAGGTGGTG AGCCTGATGT TTTCCCCACA GTCCCATTCC ATGAGGAATT TGAAAGTGGA ACAGCCAAAA AAGGGGCAGA ATCAGTCACA GAGAGAGATA CTGAAGTTGG TCATCAGGCA CATGAACATA CTGAACCTGT ATCTCTGTTT CCTGAAGAGT CTTCAGGAGA GATTGCCATT GACCAAGAAT CTCAGAAAAT AGCCTTTGCA AGGGCTACAG AAGTAACATT TGGTGAAGAG GTAGAAAAAA GTACTTCTGT CACATACACT CCCACTATAG TTCCAAGTTC TGCATCAGCA TATGTTTCAG AGGAAGAAGC AGTTACCCTA ATAGGAAATC CTTGGCCAGA TGACCTGTTG TCTACCAAAG AAAGCTGGGT AGAAGCAACT CCTAGACAAG TTGTAGAGCT CTCAGGGAGT TCTTCGATTC CAATTACAGA AGGCTCTGGA GAAGCAGAAG AAGATGAAGA TACAATGTTC ACCATGGTAA CTGATTTATC ACAGAGAAAT ACTACTGATA CACTCATTAC TTTAGACACT AGCAGGATAA TCACAGAAAG CTTTTTTGAG GTTCCTGCAA CCACCATTTA TCCAGTTTCT GAACAACCTT CTGCAAAAGT GGTGCCTACC AAGTTTGTAA GTGAAACAGA CACTTCTGAG TGGATTTCCA GTACCACTGT TGAGGAAAAG AAAAGGAAGG AGGAGGAGGG AACTACAGGT ACGGCTTCTA CATTTGAGGT ATATTCATCT ACACAGAGAT CGGATCAATT AATTTTACCC TTTGAATTAG AAAGTCCAAA TGTAGCTACA TCTAGTGATT CAGGTACCAG GAAAAGTTTT ATGTCCTTGA CAACACCAAC ACAGTCTGAA AGGGAAATGA CAGATTCTAC TCCTGTCTTT ACAGAAACAA ATACATTAGA AAATTTGGGG GCACAGACCA CTGAGCACAG CAGTATCCAT CAACCTGGGG TTCAGGAAGG GCTGACCACT CTCCCACGTA GTCCTGCCTC TGTCTTTATG GAGCAGGGCT CTGGAGAAGC TGCTGCCGAC CCAGAAACCA CCACTGTTTC TTCATTTTCA TTAAACGTAG AGTATGCAAT TCAAGCCGAA AAGGAAGTAG CTGGCACTTT GTCTCCGCAT GTGGAAACTA CATTCTCCAC TGAGCCAACA GGACTGGTTT TGAGTACAGT AATGGACAGA GTAGTTGCTG AAAATATAAC CCAAACATCC AGGGAAATAG TGATTTCAGA GCGATTAGGA GAACCAAATT ATGGGGCAGA AATAAGGGGC TTTTCCACAG GTTTTCCTTT GGAGGAAGAT TTCAGTGGTG ACTTTAGAGA ATACTCAACA GTGTCTCATC CCATAGCAAA AGAAGAAACG GTAATGATGG AAGGCTCTGG AGATGCAGCA TTTAGGGACA CCCAGACTTC ACCATCTACA GTACCTACTT CAGTTCACAT CAGTCACATA TCTGACTCAG AAGGACCCAG TAGCACCATG GTCAGCACTT CAGCCTTCCC CTGGGAAGAG TTTACATCCT CAGCTGAGGG CTCAGGTGAG CAACTGGTCA CAGTCAGCAG CTCTGTTGTT CCAGTGCTTC CCAGTGCTGT GCAAAAGTTT TCTGGTACAG CTTCCTCCAT TATCGACGAA GGATTGGGAG AAGTGGGTAC TGTCAATGAA ATTGATAGAA GATCCACCAT TTTACCAACA GCAGAAGTGG AAGGTACGAA AGCTCCAGTA GAGAAGGAGG AAGTAAAGGT CAGTGGCACA GTTTCAACAA ACTTTCCCCA AACTATAGAG CCAGCCAAAT TATGGTCTAG GCAAGAAGTC AACCCTGTAA GACAAGAAAT TGAAAGTGAA ACAACATCAG AGGAACAAAT TCAAGAAGAA AAGTCATTTG AATCCCCTCA AAACTCTCCT GCAACAGAAC AAACAATCTT TGATTCACAG ACATTTACTG AAACTGAACT CAAAACCACA GATTATTCTG TACTAACAAC AAAGAAAACT TACAGTGATG ATAAAGAAAT GAAGGAGGAA GACACTTCTT TAGTTAACAT GTCTACTCCA GATCCAGATG CAAATGGCTT GGAATCTTAC ACAACTCTCC CTGAAGCTAC TGAAAAGTCA CATTTTTTCT TAGCTACTGC ATTAGTAACT GAATCTATAC CAGCTGAACA TGTAGTCACA GATTCACCAA TCAAAAAGGA AGAAAGTACA AAACATTTTC CGAAAGGCAT GAGACCAACA ATTCAAGAGT CAGATACTGA GCTCTTATTC TCTGGACTGG GATCAGGAGA AGAAGTTTTA CCTACTCTAC CAACAGAGTC AGTGAATTTT ACTGAAGTGG AACAAATCAA TAACACATTA TATCCCCACA CTTCTCAAGT GGAAAGTACC TCAAGTGACA AAATTGAAGA CTTTAACAGA ATGGAAAATG TGGCAAAAGA AGTTGGACCA CTCGTATCTC AAACAGACAT CTTTGAAGGT AGTGGGTCAG TAACCAGCAC AACATTAATA GAAATTTTAA GTGACACTGG AGCAGAAGGA CCCACGGTGG CACCTCTCCC TTTCTCCACG GACATCGGAC ATCCTCAAAA TCAGACTGTC AGGTGGGCAG AAGAAATCCA GACTAGTAGA CCACAAACCA TAACTGAACA AGACTCTAAC AAGAATTCTT CAACAGCAGA AATTAACGAA ACAACAACCT CATCTACTGA TTTTCTGGCT AGAGCTTATG GTTTTGAAAT GGCCAAAGAA TTTGTTACAT CAGCACCAAA ACCATCTGAC TTGTATTATG AACCTTCTGG AGAAGGATCT GGAGAAGTGG ATATTGTTGA TTCATTTCAC ACTTCTGCAA CTACTCAGGC AACCAGACAA GAAAGCAGCA CCACATTTGT TTCTGATGGG TCCCTGGAAA AACATCCTGA GGTGCCAAGC GCTAAAGCTG TTACTGCTGA TGGATTCCCA ACAGTTTCAG TGATGCTGCC TCTTCATTCA GAGCAGAACA AAAGCTCCCC TGATCCAACT AGCACACTGT CAAATACAGT GTCATATGAG AGGTCCACAG ACGGTAGTTT CCAAGACCGT TTCAGGGAAT TCGAGGATTC CACCTTAAAA CCTAACAGAA AAAAACCCAC TGAAAATATT ATCATAGACC TGGACAAAGA GGACAAGGAT TTAATATTGA CAATTACAGA GAGTACCATC CTTGAAATTC TACCTGAGCT GACATCGGAT AAAAATACTA TCATAGATAT TGATCATACT AAACCTGTGT ATGAAGACAT TCTTGGAATG CAAACAGATA TAGATACAGA GGTACCATCA GAACCACATG ACAGTAATGA TGAAAGTAAT GATGACAGCA CTCAAGTTCA AGAGATCTAT GAGGCAGCTG TCAACCTTTC TTTAACTGAG GAAACATTTG AGGGCTCTGC TGATGTTCTG GCTAGCTACA CTCAGGCAAC ACATGATGAA TCAATGACTT ATGAAGATAG AAGCCAACTA GATCACATGG GCTTTCACTT CACAACTGGG ATCCCTGCTC CTAGCACAGA AACAGAATTA GACGTTTTAC TTCCCACGGC AACATCCCTG CCAATTCCTC GTAAGTCTGC CACAGTTATT CCAGAGATTG AAGGAATAAA AGCTGAAGCA AAAGCCCTGG ATGACATGTT TGAATCAAGC ACTTTGTCTG ATGGTCAAGC TATTGCAGAC CAAAGTGAAA TAATACCAAC ATTGGGCCAA TTTGAAAGGA CTCAGGAGGA GTATGAAGAC AAAAAACATG CTGGTCCTTC TTTTCAGCCA GAATTCTCTT CAGGAGCTGA GGAGGCATTA GTAGACCATA CTCCCTATCT AAGTATTGCT ACTACCCACC TTATGGATCA GAGTGTAACA GAGGTGCCTG ATGTGATGGA AGGATCCAAT CCCCCATATT ACACTGATAC AACATTAGCA GTTTCAACAT TTGCGAAGTT GTCTTCTCAG ACACCATCAT CTCCCCTCAC TATCTACTCA GGCAGTGAAG CCTCTGGACA CACAGAGATC CCCCAGCCCA GTGCTCTGCC AGGAATAGAC GTCGGCTCAT CTGTAATGTC CCCACAGGAT TCTTTTAAGG AAATTCATGT AAATATTGAA GCGACTTTCA AACCATCAAG TGAGGAATAC CTTCACATAA CTGAGCCTCC CTCTTTATCT CCTGACACAA AATTAGAACC TTCAGAAGAT GATGGTAAAC CTGAGTTATT AGAAGAAATG GAAGCTTCTC CCACAGAACT TATTGCTGTG GAAGGAACTG AGATTCTCCA AGATTTCCAA AACAAAACCG ATGGTCAAGT TTCTGGAGAA GCAATCAAGA TGTTTCCCAC CATTAAAACA CCTGAGGCTG GAACTGTTAT TACAACTGCC GATGAAATTG AATTAGAAGG TGCTACACAG TGGCCACACT CTACTTCTGC TTCTGCCACC TATGGGGTCG AGGCAGGTGT GGTGCCTTGG CTAAGTCCAC AGACTTCTGA GAGGCCCACG CTTTCTTCTT CTCCAGAAAT AAACCCTGAA ACTCAAGCAG CTTTAATCAG AGGGCAGGAT TCCACGATAG CAGCATCAGA ACAGCAAGTG GCAGCGAGAA TTCTTGATTC CAATGATCAG GCAACAGTAA ACCCTGTGGA ATTTAATACT GAGGTTGCAA CACCACCATT TTCCCTTCTG GAGACTTCTA ATGAAACAGA TTTCCTGATT GGCATTAATG AAGAGTCAGT GGAAGGCACG GCAATCTATT TACCAGGACC TGATCGCTGC AAAATGAACC CGTGCCTTAA CGGAGGCACC TGTTATCCTA CTGAAACTTC CTACGTATGC ACCTGTGTGC CAGGATACAG CGGAGACCAG TGTGAACTTG ATTTTGATGA ATGTCACTCT AATCCCTGTC GTAATGGAGC CACTTGTGTT GATGGTTTTA ACACATTCAG GTGCCTCTGC CTTCCAAGTT ATGTTGGTGC ACTTTGTGAG CAAGATACCG AGACATGTGA CTATGGCTGG CACAAATTCC AAGGGCAGTG CTACAAATAC TTTGCCCATC GACGCACATG GGATGCAGCT GAACGGGAAT GCCGTCTGCA GGGTGCCCAT CTCACAAGCA TCCTGTCTCA CGAAGAACAA ATGTTTGTTA ATCGTGTGGG CCATGATTAT CAGTGGATAG GCCTCAATGA CAAGATGTTT GAGCATGACT TCCGTTGGAC TGATGGCAGC ACACTGCAAT ACGAGAATTG GAGACCCAAC CAGCCAGACA GCTTCTTTTC TGCTGGAGAA GACTGTGTTG TAATCATTTG GCATGAGAAT GGCCAGTGGA ATGATGTTCC CTGCAATTAC CATCTCACCT ATACGTGCAA GAAAGGAACA GTCGCTTGCG GCCAGCCCCC TGTTGTAGAA AATGCCAAGA CCTTTGGAAA GATGAAACCT CGTTATGAAA TCAACTCCCT GATTAGATAC CACTGCAAAG ATGGTTTCAT TCAACGTCAC CTTCCAACTA TCCGGTGCTT AGGAAATGGA AGATGGGCTA TACCTAAAAT TACCTGCATG AACCCATCTG CATACCAAAG GACTTATTCT ATGAAATACT TTAAAAATTC CTCATCAGCA AAGGACAATT CAATAAATAC ATCCAAACAT GATCATCGTT GGAGCCGGAG GTGGCAGGAG TCGAGGCGCT CAGGCCTCAT GGGCCCAGCT TTCTTGTACA AAGTTGGCAT TATAAAAAAT AATTGCTCAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGCCTCC TCTAGACCAG CCAGGACAGA AATGCCTCGA CTTCGCTGCT GCCCAAGGTT GCCGGGTGAC GCACACCGTG GAAACGGATG AAGGCACGAA CCCAGTGGAC ATAAGCCTGT TCGGTTCGTA AGCTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA TGACTGTTTT TTTGGGGTAC AGTCTATGCC TCGGGCATCC AAGCAGCAAG CGCGTTACGC CGTGGGTCGA TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC TAAAACAAAG TTAAACATCA TGAGGGAAGC GGTGATCGCC GAAGTATCGA CTCAACTATC AGAGGTAGTT GGCGTCATCG AGCGCCATCT CGAACCGACG TTGCTGGCCG TACATTTGTA CGGCTCCGCA GTGGATGGCG GCCTGAAGCC ACACAGTGAT ATTGATTTGC TGGTTACGGT GACCGTAAGG CTTGATGAAA CAACGCGGCG AGCTTTGATC AACGACCTTT TGGAAACTTC GGCTTCCCCT GGAGAGAGCG AGATTCTCCG CGCTGTAGAA GTCACCATTG TTGTGCACGA CGACATCATT CCGTGGCGTT ATCCAGCTAA GCGCGAACTG CAATTTGGAG AATGGCAGCG CAATGACATT CTTGCAGGTA TCTTCGAGCC AGCCACGATC GACATTGATC TGGCTATCTT GCTGACAAAA GCAAGAGAAC ATAGCGTTGC CTTGGTAGGT CCAGCGGCGG AGGAACTCTT TGATCCGGTT CCTGAACAGG ATCTATTTGA GGCGCTAAAT GAAACCTTAA CGCTATGGAA CTCGCCGCCC GACTGGGCTG GCGATGAGCG AAATGTAGTG CTTACGTTGT CCCGCATTTG GTACAGCGCA GTAACCGGCA AAATCGCGCC GAAGGATGTC GCTGCCGACT GGGCAATGGA GCGCCTGCCG GCCCAGTATC AGCCCGTCAT ACTTGAAGCT AGACAGGCTT ATCTTGGACA AGAAGAAGAT CGCTTGGCCT CGCGCGCAGA TCAGTTGGAA GAATTTGTCC ACTACGTGAA AGGCGAGATC ACCAAGGTAG TCGGCAAATA ACCCTCGAGC CACCCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAA
