pENTR223.1-POLQ(human)
$157.00
In stock
SKU
PL46644
NM_199420.4;PRO0327
100μg liquid plasmid(Instantly):$178
| name | pENTR223.1-POLQ(human) | another name | apENTR223.1-POLQ(human) |
| Insert Size (bp) | 7774bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGAATCTT CTGCGTCGGA GTGGGAAACG GCGGCGTTCA GAATCAGGCT CAGATTCGTT CTCGGGAAGC GGCGGTGACA GCAGTGCCAG CCCCCAGTTC CTCTCCGGGT CCGTGCTGAG CCCGCCGCCC GGCCTTGGTC GCTGCCTGAA GGCCGCAGCT GCAGGAGAAT GCAAGCCTAC AGTTCCTGAC TACGAAAGAG ACAAGCTACT ATTGGCAAAC TGGGGACTTC CTAAAGCAGT TCTGGAAAAA TACCACAGTT TTGGTGTAAA AAAGATGTTT GAATGGCAGG CAGAGTGCCT TTTGCTTGGA CAAGTCCTGG AAGGAAAGAA TTTAGTTTAT TCAGCTCCTA CAAGTGCTGG GAAGACTCTT GTGGCAGAAT TACTTATTTT GAAGCGGGTT TTGGAAATGC GGAAGAAAGC TTTGTTTATT CTTCCCTTTG TTTCTGTGGC TAAAGAGAAG AAATACTACC TCCAGAGTCT GTTTCAGGAA GTAGGAATAA AAGTAGACGG TTATATGGGC AGCACCTCTC CATCAAGGCA TTTCTCTTCA TTGGATATTG CAGTCTGCAC AATTGAGAGA GCCAATGGTC TGATCAATCG CCTCATAGAG GAAAATAAGA TGGATCTGTT AGGAATGGTG GTTGTGGATG AATTACATAT GCTGGGAGAC TCTCACCGAG GGTATCTGCT GGAACTTTTG CTGACCAAGA TTTGCTATAT TACTCGGAAA TCAGCATCTT GTCAGGCAGA TCTAGCCAGT TCTCTGTCTA ATGCTGTGCA AATCGTTGGC ATGAGTGCTA CCCTTCCTAA TTTGGAGCTT GTGGCTTCCT GGTTGAATGC TGAACTCTAC CATACCGACT TTCGCCCTGT ACCGCTTTTG GAGTCAGTAA AAGTTGGAAA TTCCATATAT GACTCTTCAA TGAAACTTGT GAGGGAATTT GAGCCCATGC TACAAGTGAA GGGAGATGAG GACCATGTTG TTAGTTTATG TTATGAGACG ATTTGTGATA ACCATTCAGT ATTACTTTTT TGTCCATCAA AGAAATGGTG TGAGAAGCTG GCAGATATCA TTGCTCGAGA GTTTTATAAT CTACATCATC AAGCTGAGGG ATTGGTGAAA CCCTCTGAAT GCCCACCAGT AATTCTGGAA CAAAAAGAAC TCCTGGAAGT GATGGATCAG TTAAGACGTT TGCCTTCAGG ACTGGACTCT GTATTACAGA AAACTGTACC ATGGGGAGTA GCATTTCATC ATGCAGGTCT TACTTTTGAG GAGAGGGATA TCATTGAAGG AGCCTTTCGT CAAGGTCTCA TTCGGGTCTT GGCGGCAACT TCTACTCTTT CTTCTGGGGT GAATTTACCT GCACGTCGTG TGATTATTCG AACCCCTATT TTTGGTGGTC GACCTCTAGA TATTCTTACT TATAAGCAGA TGGTTGGCCG TGCTGGCAGG AAAGGAGTGG ACACAGTAGG CGAGAGTATC TTAATTTGTA AGAACTCTGA GAAATCAAAA GGCATAGCTC TCCTTCAGGG TTCTCTAAAG CCTGTTCGCA GCTGTCTGCA AAGACGAGAA GGAGAAGAAG TAACTGGCAG CATGATACGA GCTATTCTGG AGATAATAGT TGGTGGAGTG GCAAGTACAT CACAAGATAT GCATACTTAT GCTGCCTGCA CATTTTTGGC TGCAAGTATG AAAGAAGGGA AGCAAGGAAT TCAGAGAAAT CAAGAGTCTG TTCAGCTTGG AGCGATTGAG GCCTGTGTGA TGTGGCTACT AGAAAATGAA TTCATCCAGA GTACAGAAGC CAGTGATGGA ACAGAAGGAA AGGTGTATCA TCCAACACAT CTTGGTTCGG CCACTCTTTC TTCTTCACTT TCTCCAGCTG ATACTTTAGA TATTTTTGCT GACCTGCAAA GAGCAATGAA GGGCTTTGTT TTAGAGAATG ATCTTCATAT TCTCTATCTG GTTACACCTA TGTTTGAGGA TTGGACTACT ATTGATTGGT ATCGATTTTT CTGTTTATGG GAGAAGTTGC CAACTTCAAT GAAAAGGGTG GCAGAGCTAG TGGGAGTTGA AGAGGGGTTC TTGGCCCGTT GTGTGAAAGG AAAAGTAGTA GCCAGAACTG AGAGACAGCA TCGACAAATG GCCATCCATA AAAGGTTTTT CACCAGTCTT GTGCTATTAG ATTTAATCAG TGAAGTTCCC TTAAGGGAAA TAAATCAGAA ATATGGATGC AATCGTGGGC AGATTCAATC TTTGCAACAG TCAGCTGCTG TTTATGCAGG GATGATTACA GTATTTTCCA ACCGTCTGGG CTGGCACAAC ATGGAACTAC TACTTTCCCA ATTTCAGAAG CGTCTTACGT TTGGCATCCA GAGGGAGCTG TGTGACCTGG TTCGGGTATC CTTACTAAAT GCTCAGAGAG CCAGGGTTCT CTATGCTTCT GGCTTTCATA CTGTGGCAGA CCTTGCTAGA GCAAATATTG TGGAGGTGGA GGTGATTCTG AAAAATGCTG TGCCTTTCAA AAGTGCCCGG AAGGCAGTGG ATGAGGAAGA GGAAGCAGTT GAAGAACGTC GCAATATGCG AACTATCTGG GTGACTGGCA GAAAAGGTTT AACTGAAAGG GAAGCAGCAG CCCTTATAGT GGAAGAAGCC AGAATGATTC TGCAGCAGGA CTTAGTTGAA ATGGGAGTGC AATGGAATCC ATGTGCCCTG TTACATTCTA GTACATGCTC ATTGACTCAT AGTGAGTCCG AAGTAAAGGA ACACACATTT ATATCCCAAA CTAAGAGTTC TTATAAAAAA TTAACATCAA AGAACAAAAG TAACACAATA TTTAGTGATT CTTATATTAA GCATTCACCA AATATAGTGC AAGACTTAAA TAAAAGTAGA GAGCATACAA GTTCCTTTAA TTGTAATTTC CAGAATGGGA ATCAAGAACA TCAGACATGT TCCATTTTCA GAGCAAGAAA ACGGGCCTCT TTAGATATAA ATAAAGAGAA GCCAGGAGCC TCTCAGAATG AGGGGAAAAC AAGTGATAAG AAAGTTGTTC AGACTTTTTC ACAGAAAACA AAAAAGGCAC CTTTGAATTT CAATTCAGAA AAGATGAGCA GAAGTTTTCG ATCTTGGAAA CGTAGAAAGC ATCTAAAGCG ATCTAGGGAC AGCAGCCCCC TGAAAGACTC TGGAGCGTGT AGAATCCATT TACAAGGACA GACTCTGTCT AATCCTAGTC TTTGTGAAGA CCCGTTTACC TTAGATGAGA AGAAAACGGA ATTTAGAAAT TCAGGGCCAT TTGCTAAAAA TGTATCTTTG AGTGGTAAGG AAAAAGATAA TAAAACATCA TTCCCATTAC AAATAAAGCA AAATTGTTCA TGGAACATAA CACTAACTAA TGATAATTTT GTGGAGCATA TTGTCACAGG ATCTCAGAGT AAAAATGTGA CTTGTCAGGC CACTAGTGTG GTTAGTGAAA AGGGCAGAGG AGTAGCTGTT GAGGCAGAAA AAATAAATGA AGTGCTGATA CAAAATGGTT CAAAAAACCA GAATGTTTAT ATGAAACACC ATGACATCCA TCCAATTAAC CAGTACCTGC GAAAGCAATC TCATGAACAG ACAAGCACTA TTACCAAACA GAAAAATATA ATAGAGAGAC AAATGCCCTG TGAAGCAGTC AGTAGTTACA TAAATAGAGA CTCAAATGTT ACTATCAATT GTGAAAGGAT AAAGCTTAAT ACAGAGGAAA ATAAACCAAG TCATTTTCAG GCATTAGGAG ATGATATAAG CAGAACTGTG ATACCCAGTG AAGTACTTCC ATCAGCTGGA GCATTTAGCA AATCAGAAGG CCAGCATGAG AATTTTCTAA ATATTTCTAG ACTACAAGAA AAAACAGGTA CTTATACAAC AAACAAAACT AAAAATAATC ATGTTTCTGA CTTAGGTTTA GTCCTCTGTG ATTTTGAAGA TAGTTTCTAT CTGGATACTC AGTCAGAGAA AATAATACAA CAGATGGCAA CTGAAAATGC CAAACTAGGA GCAAAGGACA CCAACCTGGC AGCAGGGATA ATGCAGAAGA GCTTAGTCCA ACAGAACTCA ATGAACTCTT TTCAGAAGGA GTGTCACATT CCTTTTCCTG CTGAACAGCA CCCTCTAGGA GCGACTAAGA TAGATCATTT GGACCTTAAG ACTGTAGGTA CTATGAAACA AAGCAGTGAT TCACATGGGG TTGATATCCT GACTCCAGAA AGCCCGATTT TCCATTCTCC AATACTATTG GAGGAAAATG GTCTTTTTTT AAAAAAGAAT GAAGTTTCTG TTACTGATTC ACAATTAAAT AGTTTTCTTC AAGGTTATCA AACACAAGAA ACTGTGAAAC CAGTTATACT TCTGATTCCT CAAAAGAGAA CTCCCACTGG TGTAGAAGGA GAATGTCTTC CAGTTCCTGA AACAAGTTTG AATATGAGTG ATAGTTTACT ATTTGATAGC TTCAGTGATG ACTATCTAGT AAAAGAACAA TTACCTGATA TGCAAATGAA AGAACCCCTT CCTTCAGAAG TAACATCAAA CCATTTTAGT GATTCTCTGT GTCTACAAGA AGACCTAATT AAAAAATCAA ATGTAAATGA GAATCAAGAT ACCCACCAGC AGTTGACTTG TTCCAATGAT GAATCTATTA TATTTTCAGA AATGGATTCT GTTCAGATGG TTGAAGCTTT GGACAATGTG GATATATTTC CTGTCCAAGA GAAGAATCAT ACTGTAGTAT CTCCTAGAGC ATTAGAACTA AGTGATCCAG TACTTGATGA GCACCACCAA GGTGATCAAG ATGGAGGAGA TCAAGATGAA AGGGCTGAAA AATCAAAATT AACTGGGACC AGGCAAAATC ATTCATTCAT ATGGTCAGGG GCATCATTTG ATCTAAGTCC AGGACTGCAA AGGATTTTAG ATAAAGTATC CAGTCCTCTA GAAAATGAAA AGCTAAAATC AATGACTATA AACTTTTCCA GTTTGAATAG AAAAAATACA GAGTTAAATG AAGAACAAGA AGTTATTTCA AACTTGGAGA CAAAACAAGT GCAGGGAATT TCATTTTCTT CTAATAATGA AGTAAAAAGC AAGATTGAGA TGCTAGAAAA CAATGCCAAT CATGATGAAA CCTCATCCCT CTTACCTCGT AAAGAAAGTA ATATAGTTGA TGATAATGGT CTCATTCCTC CTACACCCAT TCCAACATCT GCTTCTAAGC TGACATTTCC AGGGATTCTT GAAACACCTG TAAACCCTTG GAAAACTAAT AATGTTTTAC AACCTGGTGA AAGTTATTTA TTTGGCTCAC CTTCAGATAT TAAAAACCAC GATTTAAGTC CAGGGAGTAG AAATGGGTTC AAAGACAACA GCCCTATTAG TGACACAAGC TTTTCACTTC AGTTATCACA GGATGGATTA CAGTTAACTC CAGCCTCAAG CAGTTCAGAA AGTTTGTCCA TAATTGATGT AGCAAGTGAC CAAAATCTTT TCCAAACATT CATTAAGGAG TGGCGGTGCA AAAAGCGATT TTCCATCTCA CTGGCTTGTG AAAAGATTAG AAGTTTGACA TCTTCTAAAA CTGCTACTAT TGGCAGTAGG TTTAAGCAAG CTAGCTCACC TCAGGAAATT CCTATTAGAG ATGATGGATT TCCCATTAAA GGTTGTGATG ACACCTTGGT GGTTGGACTG GCAGTATGCT GGGGTGGAAG GGATGCCTAT TATTTTTCAC TGCAGAAGGA ACAAAAGCAT TCTGAAATTA GTGCCAGTTT GGTTCCACCT TCTTTAGATC CAAGCCTGAC TTTGAAAGAC AGGATGTGGT ACCTTCAATC TTGCTTGCGA AAGGAATCTG ATAAAGAATG TTCTGTTGTC ATCTATGACT TCATCCAGAG CTATAAAATT CTTCTTCTTT CTTGTGGCAT CTCCTTGGAG CAAAGTTATG AAGATCCTAA GGTGGCATGC TGGTTACTAG ATCCAGATTC TCAGGAGCCG ACTCTTCATA GCATAGTTAC CAGTTTTCTT CCTCATGAGC TTCCACTCCT AGAAGGGATG GAGACCAGCC AAGGGATTCA AAGCCTGGGG CTAAATGCTG GCAGTGAGCA TTCTGGGCGA TACAGAGCAT CTGTGGAGTC CATTCTCATC TTCAACTCTA TGAATCAGCT CAACTCTTTG TTGCAGAAGG AAAACCTTCA AGATGTTTTC CGTAAGGTGG AAATGCCCTC TCAGTACTGC TTGGCCTTGC TAGAACTAAA TGGAATTGGC TTTAGTACTG CAGAATGTGA AAGTCAGAAA CATATAATGC AAGCCAAGCT GGATGCAATT GAGACCCAGG CCTATCAACT AGCTGGCCAC AGTTTTTCTT TCACCAGTTC AGATGACATC GCTGAGGTTT TATTTTTGGA ATTGAAGTTG CCCCCAAATA GAGAGATGAA AAACCAAGGC AGCAAGAAAA CTCTGGGTTC TACCAGAAGA GGGATTGACA ATGGACGCAA GCTAAGGCTG GGAAGACAGT TCAGCACTAG TAAGGACGTT TTAAATAAAT TAAAGGCATT ACATCCTTTA CCAGGCTTGA TATTAGAATG GAGAAGAATC ACTAATGCTA TTACCAAAGT GGTCTTTCCC CTTCAGCGGG AAAAGTGTCT TAATCCTTTT CTTGGAATGG AAAGAATCTA TCCTGTATCA CAGTCGCACA CTGCTACAGG ACGAATAACC TTTACAGAAC CAAATATTCA GAATGTGCCA AGAGATTTTG AAATCAAAAT GCCAACACTA GTAGGAGAAA GCCCACCTTC TCAAGCTGTA GGCAAAGGCC TACTTCCCAT GGGCAGAGGA AAATATAAGA AGGGTTTCAG CGTGAATCCT AGATGCCAGG CACAGATGGA GGAGAGAGCT GCAGACAGAG GAATGCCATT TTCAATTAGC ATGCGACATG CCTTTGTGCC TTTCCCAGGT GGTTCAATAC TGGCTGCTGA CTACTCTCAG CTTGAACTGA GGATCTTGGC TCATTTATCC CATGATCGTC GTCTCATTCA AGTGTTAAAC ACTGGAGCTG ATGTTTTCAG GAGCATTGCA GCAGAGTGGA AGATGATTGA GCCAGAGTCT GTTGGGGATG ATCTGAGGCA GCAGGCAAAA CAGATTTGCT ATGGGATCAT TTATGGAATG GGAGCTAAAT CTTTGGGAGA GCAGATGGGC ATTAAAGAAA ATGATGCTGC ATGCTATATT GACTCCTTCA AATCCAGATA CACAGGGATT AATCAATTCA TGACAGAGAC AGTGAAGAAT TGTAAAAGAG ACGGATTTGT TCAGACCATT TTGGGAAGGC GTAGATATTT GCCAGGAATC AAAGACAACA ACCCTTATCG TAAAGCTCAC GCTGAGCGTC AAGCTATCAA CACAATAGTC CAAGGATCAG CAGCTGATAT TGTCAAAATA GCCACAGTTA ACATTCAGAA GCAATTAGAG ACCTTCCACT CAACCTTCAA ATCCCATGGT CATCGAGAGG GTATGCTCCA AAGTGACCAA ACAGGATTGT CACGAAAGAG AAAACTGCAA GGGATGTTCT GCCCAATCAG AGGAGGCTTC TTCATCCTTC AACTCCATGA TGAACTCCTA TATGAAGTGG CAGAAGAAGA TGTTGTTCAG GTAGCTCAGA TTGTCAAGAA TGAAATGGAA AGTGCTGTAA AACTGTCTGT GAAATTGAAA GTGAAAGTGA AAATAGGCGC CAGCTGGGGA GAGCTAAAGG ACTTTGATGT GTCAGGCCTC ATGGGCCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAAAA ATAATTGCTC ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAAATATAT CATCATGCCT CCTCTAGACC AGCCAGGACA GAAATGCCTC GACTTCGCTG CTGCCCAAGG TTGCCGGGTG ACGCACACCG TGGAAACGGA TGAAGGCACG AACCCAGTGG ACATAAGCCT GTTCGGTTCG TAAGCTGTAA TGCAAGTAGC GTATGCGCTC ACGCAACTGG TCCAGAACCT TGACCGAACG CAGCGGTGGT AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGGGGT ACAGTCTATG CCTCGGGCAT CCAAGCAGCA AGCGCGTTAC GCCGTGGGTC GATGTTTGAT GTTATGGAGC AGCAACGATG TTACGCAGCA GGGCAGTCGC CCTAAAACAA AGTTAAACAT CATGAGGGAA GCGGTGATCG CCGAAGTATC GACTCAACTA TCAGAGGTAG TTGGCGTCAT CGAGCGCCAT CTCGAACCGA CGTTGCTGGC CGTACATTTG TACGGCTCCG CAGTGGATGG CGGCCTGAAG CCACACAGTG ATATTGATTT GCTGGTTACG GTGACCGTAA GGCTTGATGA AACAACGCGG CGAGCTTTGA TCAACGACCT TTTGGAAACT TCGGCTTCCC CTGGAGAGAG CGAGATTCTC CGCGCTGTAG AAGTCACCAT TGTTGTGCAC GACGACATCA TTCCGTGGCG TTATCCAGCT AAGCGCGAAC TGCAATTTGG AGAATGGCAG CGCAATGACA TTCTTGCAGG TATCTTCGAG CCAGCCACGA TCGACATTGA TCTGGCTATC TTGCTGACAA AAGCAAGAGA ACATAGCGTT GCCTTGGTAG GTCCAGCGGC GGAGGAACTC TTTGATCCGG TTCCTGAACA GGATCTATTT GAGGCGCTAA ATGAAACCTT AACGCTATGG AACTCGCCGC CCGACTGGGC TGGCGATGAG CGAAATGTAG TGCTTACGTT GTCCCGCATT TGGTACAGCG CAGTAACCGG CAAAATCGCG CCGAAGGATG TCGCTGCCGA CTGGGCAATG GAGCGCCTGC CGGCCCAGTA TCAGCCCGTC ATACTTGAAG CTAGACAGGC TTATCTTGGA CAAGAAGAAG ATCGCTTGGC CTCGCGCGCA GATCAGTTGG AAGAATTTGT CCACTACGTG AAAGGCGAGA TCACCAAGGT AGTCGGCAAA TAACCCTCGA GCCACCCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAA
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