pGNRH3(Zebrafish)-EGFP-nfsB(E.coli)-SV40-Neo
$99.00
In stock
SKU
PL46570
100μg liquid plasmid(Instantly):$120
| name | pGNRH3(Zebrafish)-EGFP-nfsB(E.coli)-SV40-Neo | another name | apGNRH3(Zebrafish)-EGFP-nfsB(E.coli)-SV40-Neo |
| Insert Size (bp) | Plasmid host | Fish | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Escherichia coli | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCAAGAACTT CTCACATGAA TGTGATTGTG TTACTAACGT TATTATTTGT GATATCTTTC TGTTAAATTG CTTAAAAAAT TCATTACTCA TTTATTTTCT TTTCGGCTTA GTCCCTTTAT TAATCCGGGG TTGCAACAGC GGAATGAACC GCTAACCTAT CCAGCATGTT TCTACGCAGC GGATGCCCTT CAGCCGCAAC CCATCTCTGG GAAACATCCA CACACACTCA TTTACACTCA TACACTACAG AAAATTTAGC CTACCCAATT AACCTGTATG TCTTTGGACT TGTTAGGGAA ACCGGAGCAT CTGGAGGAAA TCAGGGAGAA CATGCAAACT CCACACAGAA ACGTCAACTG ATCCAGCCGA GGCTGGAATC AGAGACCTTC TTGCTATGAG GCGACAGCAC TACCTCCTGC GCCACTTCGT CACCCTTTCT TAAAATATTG AATTATGATA AAATCGATTA CATTTAATAT TATATTTAAA ATGTTGTGAG ACTTGAACAA AATCTTAATT CACATTTTAA AGAGATTTAT TCACCCTTGA CTTGTTATTT TGTTTGCATT TATATTTTGG TTTTGTTGAA TGCCATTTTT TAGAATGGTG GAAACCAGTA ATCATTGACT TCCGTAGTAT TAGATTTTCG TATTTTTCAT GGGTGTCAAT GGTTATCGGT TTCCAACATT TCTCAAAACA TCTTCTTTTG TATTTAAAGT AACAAAAAAA AAGGTTTCGA ACAAGCCAAG GGAGAATTAA TGACGACAGA ATTTCCATTT TGGGGTGAAC TATCCTATTA ATATTTTAAT TAGCTAAGTT ACATTCAAAA GTCTAATCAA AGGTGGCAAT AAAAGATGTT TAATGTTAAA ATTCAAGCAG CACAACTGTT TTCAAAATGT CTTCTGACAC CTGTAAAGAC ATATAAAGTA AAGGGTTTAT GAGGAAATAG AATTAAAAAG TACCATCAAA TGCTAAAATG GCTCTTATAT GATTCAGTCT GTGACACAAA CATTACCTCT TGGCTCATCT GTTCCCCTGA GTGTTGATCT GGCCATCAGA GAGTCGGTTT GAACCATTCA AAGATTATTT CATGTATTGC TGGGTTTAAC CATGTCCATT AGCAAGGTGC CCTTGTCAAA TCTTTGTTTC AGTTTTTGTA AATGCAGCCC CGAGTGTACA TTAAAACCTG CATTAGCGAC AAGTCTGCAC CTGCTCTGTG GTTGCAGGAG TCTTGATGAC GAAAGTGCAG TACATCTCAT TTTAAAAGGA CTATATGAAG GAGACCAAGA ACAGCAGAAC TCATCTTGAA CAAACACAGC AGGTAAGAGA TTCTTTACTT TCAAAATTCT GTATTGTTTT CTCAATCTAG AATTTAGACA GAGTGTGATA GAAATGTTCA TATTAATAAT AAAATACTGT ACAAACAAAA GAAATACAAA AATAGCCTAT ATCTTTTGTG AAATTACAAT TTTGCCACAT AAATTGCTGT GGGTTTTGTC AGCAAGTTCA CTGCAAAAAA AAACCTTTCT TTCTTGGTTT TTTTTTTCTA GCCCAAATTT CCTAAAATTC TTAAATCAAG AAAAATTTTC TAGACAAGCA AAGCATATTG TCTTGTTTTA AGAAAAATAT GCCAAAAAAA GTTTTTCCTT AAAACAAGCA AAGTAATCTG CCAATAGGGT AGGAAAAATA ATCCTATGTC AAAAAGAAAA ACAAGATTAT TTTGCTCTCC TCCATTGCAG ATCATTTTGC TTGTTTTAAG GAAAAACTTA CTTAATATTA GCATATTATT TCTGTAAATA AGACATTATG TTTTGCTTGC CTAAATATTT CTTGATATAA GAATTTTTTG ATATTTGGAC TAGAAACAAG ATAAAAAAAA TTAAGTAAGA GCAGGAGTTT TTGCAGTGTT GTACTTTTAT ATTTAGTGGT TTCAATTAAG GATGGTGCAA TATTAATATG TTTGAGTACT TATTAACTCT TATTATATTA TTAATAACCC TATAGGACAA TGCTTGTTGA AATTCCCATT GGAAAACCCC CTGGTATTGT TACTGGGGGT TATTATGACA CTTTTACTCT GTTATGACTT CATTTTACTC TAATAGATTT ATATCAATTA ATTGCAAATA GTAAGGAATA AAAAAAAGCT AAAATAAAAT ACAAAGCAAA ACAATACAAT GCAATTTAAA AAATGATTTT TTTATTCTTT CCTATTATTG CTACTTTTTT CATATATTTT GAGATGGAAG ACAATCCTTT ATTTTTCTCA GTTGTAAATA AAGATACTTG AAAATACTTC TTTCAGTTAG TCTACTATTC ATTTCACATA TTATAGCGAA ACTGCACATT ATATTTAGGA TTTTTTGTGC TGTTTGTTTG TGTTTGTGTG ATTTTTTTTT ACAGATACAA ATAAACAAGT GATTTCTGTG TTTTAGTTTT AGCATGGAGC TAGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGGCGGCG GAGGGAGTGG AGGAGGGGGA AGCATGGATA TCATTTCTGT CGCCTTAAAG CGTCATTCCA CTAAGGCATT TGATGCCAGC AAAAAACTTA CCCCGGAACA GGCCGAGCAG ATCAAAACGC TACTGCAATA CAGCCCATCC AGCACCAACT CCCAGCCGTG GCATTTTATT GTTGCCAGCA CGGAAGAAGG TAAAGCGCGT GTTGCCAAAT CCGCTGCCGG TAATTACGTG TTCAACGAGC GTAAAATGCT TGATGCCTCG CACGTCGTGG TGTTCTGTGC AAAAACCGCG ATGGACGATG TCTGGCTGAA GCTGGTTGTT GACCAGGAAG ATGCCGATGG CCGCTTTGCC ACGCCGGAAG CGAAAGCCGC GAACGATAAA GGTCGCAAGT TCTTCGCTGA TATGCACCGT AAAGATCTGC ATGATGATGC AGAGTGGATG GCAAAACAGG TTTATCTCAA CGTCGGTAAC TTCCTGCTCG GCGTGGCGGC TCTGGGTCTG GACGCGGTAC CCATCGAAGG TTTTGACGCC GCCATCCTCG ATGCAGAATT TGGTCTGAAA GAGAAAGGCT ACACCAGTCT GGTGGTTGTT CCGGTAGGTC ATCACAGCGT TGAAGATTTT AACGCTACGC TGCCGAAATC TCGTCTGCCG CAAAACATCA CCTTAACCGA AGTGTAAGGA TCCACCGGAT CTAGATAACT GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGT
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