pCMV-ADCY8(human)-2×StrepII-P10-mEGFP
$99.00
In stock
SKU
PL46467
NM_001115.3;AC8; ADCY3; HBAC1
100μg liquid plasmid(Instantly):$120
| name | pCMV-ADCY8(human)-2×StrepII-P10-mEGFP | another name | apCMV-ADCY8(human)-2×StrepII-P10-mEGFP |
| Insert Size (bp) | 3753bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCTCTGTCA CAGAATGAAA ATTTTTCTGT CATCTCTTCG TTATTAATGT TTGTAATTGA CTGAATATCA ACGCTTATTT GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAGACCAGC CGCGTAACCT GGCAAAATCG GTTACGGTTG AGTAATAAAT GGATGCCCTG CGTAAGCGGG TGTGGGCGGA CAATAAAGTC TTAAACTGAA CAAAATAGAT CTAAACTATG ACAATAAAGT CTTAAACTAG ACAGAATAGT TGTAAACTGA AATCAGTCCA GTTATGCTGT GAAAAAGCAT ACTGGACTTT TGTTATGGCT AAAGCAAACT CTTCATTTTC TGAAGTGCAA ATTGCCCGTC GTATTAAAGA GGGGCGTGGC CAAGGGCATG GTAAAGACTA TATTCGCGGC GTTGTGACAA TTTACCGAAC AACTCCGCGG CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT AGATATAGAT CTCACTACGC GGCTGCTCAA ACTTGGGCAG AACGTAAGCC GCGAGAGCGC CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG CCCTTGCTTC AGGAGATCGG TAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CGTACCCGTA GTGGCTATGG CAGGGCTTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGTT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGTCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC CCGGTTCCGC ATGCTATGCA TCCTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGCT TGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCGGG TGATCAAGTC TTCGTCGAGT GATTGTAAAT AAAATGTAAT TTACAGTATA GTATTTTAAT TAATATACAA ATGATTTGAT AATAATTCTT ATTTAACTAT AATATATTGT GTTGGGTTGA ATTAAAGGTC CGTATACTCC GGAATATTAA TAGGATCCCG GTCCGAGCGC GCGGAATTCA ATATTGGCCA TTAGCCATAT TATTCATTGG TTATATAGCA TAAATCAATA TTGGCTATTG GCCATTGCAT ACGTTGTATC TATATCATAA TATGTACATT TATATTGGCT CATGTCCAAT ATGACCGCCA TGTTGGCATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTCCGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA CGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACACCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ATAACCCCGC CCCGTTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCA CTAGAAGCTT TATTGCGGTA GTTTATCACA GTTAAATTGC TAACGCAGTC AGTGCTTCTG ACACAACAGT CTCGAACTTA AGCTGCAGAA GTTGGTCGTG AGGCACTGGG CAGGTAAGTA TCAAGGTTAC AAGACAGGTT TAAGGAGACC AATAGAAACT GGGCTTGTCG AGACAGAGAA GACTCTTGCG TTTCTGATAG GCACCTATTG GTCTTACTGA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG TTCAATTACA GCTCTTAAGG CTAGAGTACT TAATACGACT CACTATAGGC TAGCCTCGAG CCACCATGGA GCTCTCCGAT GTGCGCTGCC TTACAGGCAG CGAGGAACTC TACACCATCC ACCCGACGCC CCCGGCCGGC GACGGCAGGA GCGCCTCCCG GCCGCAGCGG CTGCTGTGGC AGACGGCGGT GCGACACATC ACGGAGCAGC GCTTCATTCA CGGGCACCGG GGAGGCAGCG GCAGCGGGAG TGGAGGCTCG GGCAAAGCCT CGGACCCTGC GGGCGGCGGC CCCAACCACC ACGCGCCGCA GCTGTCAGGC GACTCGGCGC TGCCCCTCTA CTCGCTGGGC CCGGGAGAGC GAGCGCACAG CACCTGCGGC ACCAAAGTCT TCCCGGAACG CAGCGGGAGC GGCAGTGCCA GCGGCAGCGG AGGCGGGGGC GACCTGGGCT TCCTGCACCT TGACTGTGCC CCTAGCAACT CGGATTTCTT TCTTAATGGG GGCTATAGCT ACCGAGGGGT CATTTTCCCC ACCCTGCGCA ACTCCTTCAA ATCTCGGGAT TTGGAACGCC TCTACCAGCG CTATTTCTTG GGCCAAAGGC GCAAATCGGA AGTGGTGATG AACGTGCTGG ACGTGCTGAC CAAACTCACT CTCTTGGTCC TACACTTGAG CCTGGCCTCG GCCCCCATGG ACCCGCTCAA GGGCATCCTG CTGGGCTTCT TCACCGGCAT TGAGGTAGTG ATCTGCGCCC TGGTGGTGGT CAGGAAGGAC ACCACCTCCC ACACGTACCT GCAGTACAGC GGCGTGGTCA CCTGGGTGGC CATGACCACC CAGATCCTGG CAGCAGGCCT CGGCTACGGG CTCCTGGGCG ACGGCATAGG CTACGTGCTC TTCACGCTCT TCGCCACCTA CAGTATGCTG CCGCTGCCGC TCACCTGGGC CATCCTGGCC GGCCTGGGCA CCTCGCTGCT GCAGGTCATC CTCCAAGTGG TCATACCCCG GCTGGCGGTC ATTTCCATCA ACCAGGTTGT GGCCCAGGCA GTGCTATTCA TGTGTATGAA CACAGCTGGA ATCTTCATCA GTTACCTGTC AGACCGGGCC CAGCGCCAAG CTTTCCTGGA GACTCGGAGG TGTGTGGAGG CCAGGCTGCG CCTGGAGACA GAGAACCAAA GACAGGAGCG GCTCGTGCTT TCTGTGCTCC CCCGGTTTGT TGTCCTGGAA ATGATCAACG ACATGACCAA TGTGGAAGAT GAGCACCTGC AGCACCAGTT CCATCGGATC TACATCCATC GCTATGAGAA CGTCAGTATT CTTTTTGCAG ATGTTAAAGG ATTTACCAAC CTCTCCACGA CCTTGTCTGC TCAGGAGCTG GTCAGGATGC TCAACGAGCT CTTTGCCAGA TTTGATCGAC TGGCCCATGA GCATCACTGC CTTCGTATTA AAATCCTGGG GGACTGCTAC TACTGCGTGT CTGGACTTCC TGAGCCCCGC CAGGACCATG CCCACTGCTG TGTTGAAATG GGTCTCAGCA TGATCAAAAC CATCAGGTAT GTGCGGTCAA GGACAAAACA CGATGTTGAC ATGAGGATTG GAATCCACTC CGGCTCGGTG CTGTGCGGTG TTTTGGGACT AAGGAAGTGG CAGTTTGATG TCTGGTCTTG GGATGTGGAT ATTGCAAACA AACTCGAATC TGGAGGAATC CCTGGGAGGA TTCACATTTC CAAAGCCACG CTGGACTGTC TCAACGGTGA CTATAACGTG GAAGAGGGCC ATGGTAAAGA GAGGAATGAA TTCCTGAGGA AGCATAATAT CGAAACTTAC TTAATTAAGC AGCCTGAGGA CAGTCTGCTG TCCTTGCCTG AAGATATCGT CAAGGAGTCA GTGAGCTCCT CAGACCGGAG AAACAGTGGG GCCACATTCA CTGAAGGATC CTGGAGCCCT GAACTGCCCT TTGATAATAT CGTGGGGAAA CAGAATACTC TGGCTGCCCT AACAAGAAAT TCAATAAATC TGCTTCCAAA CCATCTTGCA CAAGCTTTGC ATGTCCAGTC TGGGCCTGAG GAAATTAACA AGAGAATAGA ACATACCATC GACTTGCGGA GTGGCGATAA ATTGAGAAGA GAGCATATCA AGCCATTCTC ACTGATGTTT AAAGACTCCA GCCTGGAGCA CAAGTATTCT CAAATGAGGG ATGAAGTGTT CAAGTCAAAC TTGGTCTGTG CATTTATCGT TCTTCTATTT ATCACGGCAA TACAAAGTTT GCTTCCTTCT TCAAGAGTGA TGCCAATGAC CATCCAGTTC TCCATTCTGA TTATGCTGCA CTCGGCTCTG GTCCTCATCA CCACAGCAGA GGATTATAAA TGTTTGCCCC TCATCCTCCG GAAAACTTGC TGTTGGATTA ATGAGACCTA TTTGGCCCGG AACGTCATCA TCTTTGCATC CATTTTGATT AATTTCCTGG GTGCCATCTT AAATATCCTG TGGTGTGATT TTGACAAGTC GATACCCTTG AAGAACCTGA CTTTCAATTC CTCAGCTGTG TTTACAGATA TCTGCTCCTA CCCAGAGTAC TTTGTCTTCA CGGGGGTGTT GGCCATGGTG ACCTGTGCAG TTTTCCTCCG GCTGAACTCC GTCCTGAAGC TGGCAGTGCT GCTGATCATG ATTGCCATCT ATGCCCTGCT CACTGAGACC GTCTACGCAG GCCTCTTTCT GCGTTATGAC AACCTCAACC ACAGTGGAGA AGATTTCCTG GGGACCAAGG AGGTATCACT GCTACTGATG GCCATGTTCC TCCTGGCTGT GTTCTACCAT GGACAGCAGC TGGAGTACAC AGCCCGCCTG GACTTCCTTT GGCGAGTACA GGCCAAAGAG GAGATCAATG AGATGAAGGA GCTGAGGGAA CACAATGAGA ACATGCTCCG GAATATCTTA CCCAGCCATG TGGCCCGCCA TTTCCTAGAG AAGGACCGAG ACAATGAGGA GCTGTATTCT CAATCCTATG ATGCTGTTGG GGTGATGTTT GCCTCCATCC CAGGATTTGC GGACTTTTAC TCTCAGACTG AAATGAATAA CCAGGGAGTG GAATGCCTGC GCTTGCTCAA TGAGATCATT GCTGACTTCG ATGAGTTGCT TGGTGAAGAC CGATTTCAAG ACATTGAAAA GATTAAGACC ATTGGCAGCA CCTACATGGC CGTGTCAGGC CTGTCACCTG AAAAACAGCA ATGTGAAGAC AAGTGGGGAC ATTTGTGTGC TCTGGCTGAC TTCTCACTCG CCCTGACAGA AAGCATACAG GAGATCAACA AGCATTCATT CAACAATTTT GAACTCCGGA TTGGCATCAG CCACGGCTCA GTGGTAGCTG GCGTTATCGG CGCTAAGAAA CCACAGTATG ACATTTGGGG CAAAACTGTG AACCTGGCAA GCCGAATGGA CAGCACGGGG GTTAGTGGCC GGATCCAAGT CCCAGAGGAG ACCTATCTCA TCCTGAAGGA CCAGGGCTTT GCCTTTGATT ACCGAGGGGA GATCTATGTG AAGGGTATCA GTGAACAGGA AGGAAAAATC AAAACGTACT TTCTTCTGGG AAGAGTCCAA CCCAACCCAT TCATCTTGCC CCCAAGAAGA CTGCCTGGGC AGTACTCCCT GGCCGCGGTT GTCCTGGGAC TTGTCCAGTC CCTCAATAGG CAAAGGCAGA AGCAGCTACT CAATGAGAAC AACAACACAG GAATCATCAA GGGTCATTAC AACCGGCGGA CTTTGTTGTC ACCCAGCGGC ACAGAGCCTG GAGCCCAGGC TGAAGGCACC GACAAATCTG ATTTGCCAGG CAGCGCCGGC AGCGCCTGGA GCCACCCCCA GTTCGAGAAG GGCGGCGGCA GCGGCGGCGG CAGCGGCGGC AGCGCCTGGA GCCACCCCCA GTTCGAGAAG TAAGTACCAA GCTTAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG AATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGCAGT CTGACAAGCT TGTCGAGAAG TACTAGAGGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT GATCACTGCT TGAGCCTAGG AGATCCGAAC CAGATAAGTG AAATCTAGTT CCAAACTATT TTGTCATTTT TAATTTTCGT ATTAGCTTAC GACGCTACAC CCAGTTCCCA TCTATTTTGT CACTCTTCCC TAAATAATCC TTAAAAACTC CATTTCCACC CCTCCCAGTT CCCAACTATT TTGTCCGCCC ACAGCGGGGC ATTTTTCTTC CTGTTATGTT TTTAATCAAA CATCCTGCCA ACTCCATGTG ACAAACCGTC ATCTTCGGCT ACTTT
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