pY26IFNAR
$113.00
In stock
SKU
PL46130
100μg liquid plasmid(Instantly):$134
| name | pY26IFNAR | another name | apY26IFNAR |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | TEF1,GAP | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGTATGA TCCAATATCA AAGGAAATGA TAGCATTGAA GGATGAGACT AATCCAATTG AGGAGTGGCA GCATATAGAA CAGCTAAAGG GTAGTGCTGA AGGAAGCATA CGATACCCCG CATGGAATGG GATAATATCA CAGGAGGTAC TAGACTACCT TTCATCCTAC ATAAATAGAC GCATATAAGT ACGCATTTAA GCATAAACAC GCACTATGCC GTTCTTCTCA TGTATATATA TATACAGGCA ACACGCAGAT ATAGGTGCGA CGTGAACAGT GAGCTGTATG TGCGCAGCTC GCGTTGCATT TTCGGAAGCG CTCGTTTTCG GAAACGCTTT GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGAAAGTATA GGAACTTCAG AGCGCTTTTG AAAACCAAAA GCGCTCTGAA GACGCACTTT CAAAAAACCA AAAACGCACC GGACTGTAAC GAGCTACTAA AATATTGCGA ATACCGCTTC CACAAACATT GCTCAAAAGT ATCTCTTTGC TATATATCTC TGTGCTATAT CCCTATATAA CCTACCCATC CACCTTTCGC TCCTTGAACT TGCATCTAAA CTCGACCTCT ACATTTTTTA TGTTTATCTC TAGTATTACT CTTTAGACAA AAAAATTGTA GTAAGAACTA TTCATAGAGT GAATCGAAAA CAATACGAAA ATGTAAACAT TTCCTATACG TAGTATATAG AGACAAAATA GAAGAAACCG TTCATAATTT TCTGACCAAT GAAGAATCAT CAACGCTATC ACTTTCTGTT CACAAAGTAT GCGCAATCCA CATCGGTATA GAATATAATC GGGGATGCCT TTATCTTGAA AAAATGCACC CGCAGCTTCG CTAGTAATCA GTAAACGCGG GAAGTGGAGT CAGGCTTTTT TTATGGAAGA GAAAATAGAC ACCAAAGTAG CCTTCTTCTA ACCTTAACGG ACCTACAGTG CAAAAAGTTA TCAAGAGACT GCATTATAGA GCGCACAAAG GAGAAAAAAA GTAATCTAAG ATGCTTTGTT AGAAAAATAG CGCTCTCGGG ATGCATTTTT GTAGAACAAA AAAGAAGTAT AGATTCTTTG TTGGTAAAAT AGCGCTCTCG CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTTT ACAAAAATGA AGCACAGATT CTTCGTTGGT AAAATAGCGC TTTCGCGTTG CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT GTTCTACAAA ATGAAGCACA GATGCTTCGT TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT ACCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCCT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CGCTATTACG CCAGCTGAAT TGGAGCGACC TCATGCTATA CTGAGAAAGC AACCTGACCT ACAGGAAAGA GTTACTCAAG AATAAGAATT TTCGTTTTAA AACCTAAGAG TCACTTTAAA ATTTGTATAC ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA TTTAGAAGTG TCAACAACGT ATCTACCAAC GATTTGACCC TTTTCCATCT TTTCGTAAAT TTCTGGCAAG GTAGACAAGC CGACAACCTT GATTGGAGAC TTGACCAAAC CTCTGGCGAA GAATTGTTAA TTAAAGATCT CCGCGGTTAC TCTTTCTTTC TTAATCTGTC TTGAAGATTT CTAGAGGAAG AGAAACTACG CATAACTTCT GCTCTTACTA TTTCCCATGC ACATGGAGAA TATGATTTTT CCTGTAGATA TAAGGTTAAT CTATGAAAAT ATTTACGCAC AGCCAATATA GAATCTTCTT CTAGTAATGG AGTACCTTCT AAACCCGCTT CTTGCATAAC GCAAGCTTCT AGATCTCTTA GCTGTTGGTC CAATCCAGTA CAGAATTGAT GCAATAATGA TTCGTTCCAT GCGGCGGCTG AACCTTCTGT GGAAAACAAT TGAAATGTCT GCTGTAACAT TTCGTGAACT AAGGCCATAG CCTGAGCTTT CTGCACTTGA TTACCGCCAA AAGCTTCATG AGGAGATCCG AAATCTCTTC TATGATCCAA GCAAGAGAAG GGTGAGATTC TACGCATTTG AGCAAGCAGA CGCAAGGCTC TTGTATGAGC TAGTGAATGT GTTTGTGGCA AGTCACATTC AGCCTCTCTC TTCTCGAGAG ATACCCCTTC TTCTTTAGCA GCAATGCTGG CAATAGTAGT ATTTATAAAC AATAACCCGT TATTTGTGCT GTTGGAAAAT GGCAAAACAG CAACATCGAA ATCCCCTTCT AAATCTGAGT AACCGATGAC AGCTTCAGCC GGAATTTGTG CCGTTTCATC TTCTGTTGTA GTGTTGACTG GAGCAGCTAA TGCGGAGGAT GCTGCGAATA AAACAGCAGT AAAAATTGAA GGAAATCTCA TAGCGGCCGC GCTAGTTCTA GAAAACTTAG ATTAGATTGC TATGCTTTCT TTCTAATGAG CAAGAAGTAA AAAAAGTTGT AATAGAACAA GAAAAATGAA ACTGAAACTT GAGAAATTGA AGACCGTTTA TTAACTTAAA TATCAATGGG AGGTCATCGA AAGAGAAAAA AATCAAAAAA AAAATTTTCA AGAAAAAGAA ACGTGATAAA AATTTTTATT GCCTTTTTCG ACGAAGAAAA AGAAACGAGG CGGTCTCTTT TTTCTTTTCC AAACCTTTAG TACGGGTAAT TAACGACACC CTAGAGGAAG AAAGAGGGGA AATTTAGTAT GCTGTGCTTG GGTGTTTTGA AGTGGTACGG CGATGCGCGG AGTCCGAGAA AATCTGGAAG AGTAAAAAAG GAGTAGAAAC ATTTTGAAGC TATGAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT GCGCGCCGAG CTCAGTTTAT CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATTC TAGAACTAGT ATGAGATTTC CTTCAATTTT TACTGCTGTT TTATTCGCAG CATCCTCCGC ATTAGCTGCT CCAGTCAACA CTACAACAGA AGATGAAACG GCACAAATTC CGGCTGAAGC TGTCATCGGT TACTCAGATT TAGAAGGGGA TTTCGATGTT GCTGTTTTGC CATTTTCCAA CAGCACAAAT AACGGGTTAT TGTTTATAAA TACTACTATT GCCAGCATTG CTGCTAAAGA AGAAGGGGTA TCTCTCGAGA AGAGAGAGGC TGAAGTTCCT GTACCTGAAG CATTAAGAGC ATTGCCCGGT GCTAGAGGTT GTCATCTAGC CCAATTCAAG TCTTTGAGTC CACAAGCTTT GCAAGCTTTC AAGAGAGCTA AAGATGCATT TGAGGAATCA TTACTTGAAG ACTGGAACTG TTCATCAAGA ATTTTTCCAA GAAGTAGAGA TTTGAAACAA CTACAAGTTT GGGAACGTCC AGTAGCATTG GAAGCGGAGG TAGCTCTAAC CCTGTCCGTA TTGGGATCTC TGGCAAATAG TTCTTTGCAT AGTAGCCTTG ACCAGCCCTT GCATACTTTA AGACATATAC ACGCTCAATT ACAAGCATGC GTTCCCGCAC AACCTATGGC CGGACCAAGG CCAAGAGGTA GACTTCACCA TTGGCTTCAT AGATTACAAG AAGCGCAAAA AAAGGAACCA CAGTCATGTC TGGAAGCTAG TGTGATGTTC AACTTGTTTC GTCTTTTGAC TAGAGATTTG AAGTGTGTTG CTTCTGGCGA TCTATGTGTT TAAGATATCA AGCTTATCGA TACCGTCGAC CTCGAGTCAT GTAATTAGTT ATGTCACGCT TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA GGAAGGAGTT AGACAACCTG AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT ATTAAGAACG TTATTTATAT TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCGGCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAG GGTAATAACT GATATAATTA AATTGAAGCT CTAATTTGTG AGTTTAGTAT ACATGCATTT ACTTATAATA CAGTTTTTCA TGAGATGCCT GCAAGCAATT CGTTCTGTAT CAGGCGCAGG AGCGTCCCGT CCGGGTCGAC CAAAGCGGCC ATCGTGCCTC CCCACTCCTG CAGTTCGGGG GCATGGATGC GCGGATAGCC GCTGCTGGTT TCCTGGATGC CGACGGATTT GCACTGCCGG TAGAACTCCG CGAGGTCGTC CAGCCTCAGG CAGCAGCTGA ACCAACTCGC GAGGGGATCG AGCCCGGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAATTCGAG CTCGCCCTTA GATTAGATTG CTATGCTTTC TTTCTAATGA GCAAGAAGTA AAAAAAGTTG TAATAGAACA AGAAAAATGA AACTGAAACT TGAGAAATTG AAGACCGTTT ATTAACTTAA ATATCAATGG GAGGTCATCG AAAGAGAAAA AAATCAAAAA AAAAAATTTT CAAGAAAAAG AAACGTGATA AAAATTTTTA TTGCCTTTTT CGACGAAGAA AAAGAAACGA GGCGGTCTCT TTTTTCTTTT CCAAACCTTT AGTACGGGTA ATTAACGACA CCCTAGAGGA AGAAAGAGGG GAAATTTAGT ATGCTGTGCT TGGGTGTTTT GAAGTGGTAC GGCGATGCGC GGAGTCCGAG AAAATCTGGA AGAGTAAAAA AGGAGTAGAA ACATTTTGAA GCTATGGTGT GTGGCATATG CGTATATATA CCAATCTAAG TCTGTGCTCC TTCCTTCGTT CTTCCTTCTG TTCGGAGATT ACCGAATCAA AAAAATTTCA AAGAAACCGA AATCAAAAAA AAGAATAAAA AAAAAATGAT GAATTGAATT GAAAAGCTGT GGTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGA
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