NM_014139.3;FEPS3; HSAN7; NaN; NAV1.9; PN5; SCN12A;SNS-2
100μg liquid plasmid(Instantly):$149
| name | pCMV-SCN11A(human)-EGFP-Neo | another name | apCMV-SCN11A(human)-EGFP-Neo |
| Insert Size (bp) | 5376bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCCACCATGG ATGACAGATG CTACCCAGTA ATCTTTCCAG ATGAGCGGAA TTTCCGCCCC TTCACTTCCG ACTCTCTGGC TGCAATTGAG AAGCGGATTG CCATCCAAAA GGAGAAAAAG AAGTCTAAAG ACCAGACAGG AGAAGTACCC CAGCCTCGGC CTCAGCTTGA CCTAAAGGCC TCCAGGAAGT TGCCCAAGCT CTATGGCGAC ATTCCTCGTG AGCTCATAGG AAAGCCTCTG GAAGACTTGG ACCCATTCTA CCGAAATCAT AAGACATTTA TGGTGTTAAA CAGAAAGAGG ACAATCTACC GCTTCAGTGC CAAGCATGCC TTGTTCATTT TTGGGCCTTT CAATTCAATC AGAAGTTTAG CCATTAGAGT CTCAGTCCAT TCATTGTTCA GCATGTTCAT TATCGGCACC GTTATCATCA ACTGCGTGTT CATGGCTACA GGGCCTGCTA AAAACAGCAA CAGTAACAAT ACTGACATTG CAGAGTGTGT CTTCACTGGG ATTTATATTT TTGAAGCTTT GATTAAAATA TTGGCAAGAG GTTTCATTCT GGATGAGTTT TCTTTCCTTC GAGATCCATG GAACTGGCTG GACTCCATTG TCATTGGAAT AGCGATTGTG TCATATATTC CAGGAATCAC CATCAAACTA TTGCCCCTGC GTACCTTCCG TGTGTTCAGA GCTTTGAAAG CAATTTCAGT AGTTTCACGT CTGAAGGTCA TCGTGGGGGC CTTGCTACGC TCTGTGAAGA AGCTGGTCAA CGTGATTATC CTCACCTTCT TTTGCCTCAG CATCTTTGCC CTGGTAGGTC AGCAGCTCTT CATGGGAAGT CTGAACCTGA AATGCATCTC GAGGGACTGT AAAAATATCA GTAACCCGGA AGCTTATGAC CATTGCTTTG AAAAGAAAGA AAATTCACCT GAATTCAAAA TGTGTGGCAT CTGGATGGGT AACAGTGCCT GTTCCATACA ATATGAATGT AAGCACACCA AAATTAATCC TGACTATAAT TATACGAATT TTGACAACTT TGGCTGGTCT TTTCTTGCCA TGTTCCGGCT GATGACCCAA GATTCCTGGG AGAAGCTTTA TCAACAGACC CTGCGTACTA CTGGGCTCTA CTCAGTCTTC TTCTTCATTG TGGTCATTTT CCTGGGCTCC TTCTACCTGA TTAACTTAAC CCTGGCTGTT GTTACCATGG CATATGAGGA GCAGAACAAG AATGTAGCTG CAGAGATAGA GGCCAAGGAA AAGATGTTTC AGGAAGCCCA GCAGCTGTTA AAGGAGGAAA AGGAGGCTCT GGTTGCCATG GGAATTGACA GAAGTTCACT TACTTCCCTT GAAACATCAT ATTTTACCCC AAAAAAGAGA AAGCTCTTTG GTAATAAGAA AAGGAAGTCC TTCTTTTTGA GAGAGTCTGG GAAAGACCAG CCTCCTGGGT CAGATTCTGA TGAAGATTGC CAAAAAAAGC CACAGCTCCT AGAGCAAACC AAACGACTGT CCCAGAATCT ATCACTGGAC CACTTTGATG AGCATGGAGA TCCTCTCCAA AGGCAGAGAG CACTGAGTGC TGTCAGCATC CTCACCATCA CCATGAAGGA ACAAGAAAAA TCACAAGAGC CTTGTCTCCC TTGTGGAGAA AACCTGGCAT CCAAGTACCT CGTGTGGAAC TGTTGCCCCC AGTGGCTGTG CGTTAAGAAG GTCCTGAGAA CTGTGATGAC TGACCCGTTT ACTGAGCTGG CCATCACCAT CTGCATCATC ATCAACACTG TCTTCTTGGC CATGGAGCAT CACAAGATGG AGGCCAGTTT TGAGAAGATG TTGAATATAG GGAATTTGGT TTTCACTAGC ATTTTTATAG CAGAAATGTG CCTAAAAATC ATTGCGCTCG ATCCCTACCA CTACTTTCGC CGAGGCTGGA ACATTTTTGA CAGCATTGTT GCTCTTCTGA GTTTTGCAGA TGTAATGAAC TGTGTACTTC AAAAGAGAAG CTGGCCATTC TTGCGTTCCT TCAGAGTGCT CAGGGTCTTC AAGTTAGCCA AATCCTGGCC AACTTTGAAC ACACTAATTA AGATAATCGG CAACTCTGTC GGAGCCCTTG GAAGCCTGAC TGTGGTCCTG GTCATTGTGA TCTTTATTTT CTCAGTAGTT GGCATGCAGC TTTTTGGCCG TAGCTTCAAT TCCCAAAAGA GTCCAAAACT CTGTAACCCG ACAGGCCCGA CAGTCTCATG TTTACGGCAC TGGCACATGG GGGATTTCTG GCACTCCTTC CTAGTGGTAT TCCGCATCCT CTGCGGGGAA TGGATCGAAA ATATGTGGGA ATGTATGCAA GAAGCGAATG CATCATCATC ATTGTGTGTT ATTGTCTTCA TATTGATCAC GGTGATAGGA AAACTTGTGG TGCTCAACCT CTTCATTGCC TTACTGCTCA ATTCCTTTAG CAATGAGGAA AGAAATGGAA ACTTAGAAGG AGAGGCCAGG AAAACTAAAG TCCAGTTAGC ACTGGATCGA TTCCGCCGGG CTTTTTGTTT TGTGAGACAC ACTCTTGAGC ATTTCTGTCA CAAGTGGTGC AGGAAGCAAA ACTTACCACA GCAAAAAGAG GTGGCAGGAG GCTGTGCTGC ACAAAGCAAA GACATCATTC CCCTGGTCAT GGAGATGAAA AGGGGCTCAG AGACCCAGGA GGAGCTTGGT ATACTAACCT CTGTACCAAA GACCCTGGGC GTCAGGCATG ATTGGACTTG GTTGGCACCA CTTGCGGAGG AGGAAGATGA CGTTGAATTT TCTGGTGAAG ATAATGCACA GCGCATCACA CAACCTGAGC CTGAACAACA GGCCTATGAG CTCCATCAGG AGAACAAGAA GCCCACGAGC CAGAGAGTTC AAAGTGTGGA AATTGACATG TTCTCTGAAG ATGAGCCTCA TCTGACCATA CAGGATCCCC GAAAGAAGTC TGATGTTACC AGTATACTAT CAGAATGTAG CACCATTGAT CTTCAGGATG GCTTTGGATG GTTACCTGAG ATGGTTCCCA AAAAGCAACC AGAGAGATGT TTGCCCAAAG GCTTTGGTTG CTGCTTTCCA TGCTGTAGCG TGGACAAGAG AAAGCCTCCC TGGGTCATTT GGTGGAACCT GCGGAAAACC TGCTACCAAA TAGTGAAACA CAGCTGGTTT GAGAGCTTTA TTATCTTTGT GATTCTGCTG AGCAGTGGGG CACTGATATT TGAAGATGTT CACCTTGAGA ACCAACCCAA AATCCAAGAA TTACTAAATT GTACTGACAT TATTTTTACA CATATTTTTA TCCTGGAGAT GGTACTAAAA TGGGTAGCCT TCGGATTTGG AAAGTATTTC ACCAGTGCCT GGTGCTGCCT TGATTTCATC ATTGTGATTG TCTCTGTGAC CACCCTCATT AACTTAATGG AATTGAAGTC CTTCCGGACT CTACGAGCAC TGAGGCCTCT TCGTGCGCTG TCCCAGTTTG AAGGAATGAA GGTGGTGGTC AATGCTCTCA TAGGTGCCAT ACCTGCCATT CTGAATGTTT TGCTTGTCTG CCTCATTTTC TGGCTCGTAT TTTGTATTCT GGGAGTATAC TTCTTTTCTG GAAAATTTGG GAAATGCATT AATGGAACAG ACTCAGTTAT AAATTATACC ATCATTACAA ATAAAAGTCA ATGTGAAAGT GGCAATTTCT CTTGGATCAA CCAGAAAGTC AACTTTGACA ATGTGGGAAA TGCTTACCTC GCTCTGCTGC AAGTGGCAAC ATTTAAGGGC TGGATGGATA TTATATATGC AGCTGTTGAT TCCACAGAGA AAGAACAACA GCCAGAGTTT GAGAGCAATT CACTCGGTTA CATTTACTTC GTAGTCTTTA TCATCTTTGG CTCATTCTTC ACTCTGAATC TCTTCATTGG CGTTATCATT GACAACTTCA ACCAACAGCA GAAAAAGTTA GGTGGCCAAG ACATTTTTAT GACAGAAGAA CAGAAGAAAT ACTATAATGC AATGAAAAAA TTAGGATCCA AAAAACCTCA AAAACCCATT CCACGGCCTC TGAACAAATG TCAAGGTCTC GTGTTCGACA TAGTCACAAG CCAGATCTTT GACATCATCA TCATAAGTCT CATTATCCTA AACATGATTA GCATGATGGC TGAATCATAC AACCAACCCA AAGCCATGAA ATCCATCCTT GACCATCTCA ACTGGGTCTT TGTGGTCATC TTTACGTTAG AATGTCTCAT CAAAATCTTT GCTTTGAGGC AATACTACTT CACCAATGGC TGGAATTTAT TTGACTGTGT GGTCGTGCTT CTTTCCATTG TTAGTACAAT GATTTCTACC TTGGAAAATC AGGAGCACAT TCCTTTCCCT CCGACGCTCT TCAGAATTGT CCGCTTGGCT CGGATTGGCC GAATCCTGAG GCTTGTCCGG GCTGCACGAG GAATCAGGAC TCTCCTCTTT GCTCTGATGA TGTCGCTTCC TTCTCTGTTC AACATTGGTC TTCTACTCTT TCTGATTATG TTTATCTATG CCATTCTGGG TATGAACTGG TTTTCCAAAG TGAATCCAGA GTCTGGAATC GATGACATAT TCAACTTCAA GACTTTTGCC AGCAGCATGC TCTGTCTCTT CCAGATAAGC ACATCAGCAG GTTGGGATTC CCTGCTCAGC CCCATGCTGC GATCAAAAGA ATCATGTAAC TCTTCCTCAG AAAACTGCCA CCTCCCTGGC ATAGCCACAT CCTACTTTGT CAGTTACATT ATCATCTCCT TTCTCATTGT TGTCAACATG TACATTGCTG TGATTTTAGA GAACTTCAAT ACAGCCACTG AAGAAAGTGA GGACCCTTTG GGTGAAGATG ACTTTGACAT ATTTTATGAA GTGTGGGAAA AGTTTGACCC AGAAGCAACA CAATTTATCA AATATTCTGC CCTTTCTGAC TTTGCTGATG CCTTGCCTGA GCCTTTGCGT GTCGCAAAGC CAAATAAATA TCAATTTCTA GTAATGGACT TGCCCATGGT GAGTGAAGAT CGCCTCCACT GCATGGATAT TCTTTTCGCC TTCACCGCTA GGGTACTCGG TGGCTCTGAT GGCCTAGATA GTATGAAAGC AATGATGGAA GAGAAGTTCA TGGAAGCCAA TCCTCTCAAG AAGTTGTATG AACCCATAGT CACCACCACC AAGAGAAAGG AAGAGGAAAG AGGTGCTGCT ATTATTCAAA AGGCCTTTCG AAAGTACATG ATGAAGGTGA CCAAGGGTGA CCAAGGTGAC CAAAATGACT TGGAAAACGG GCCTCATTCA CCACTCCAGA CTCTTTGCAA TGGAGACTTG TCTAGCTTTG GGGTGGCCAA GGGCAAGGTC CACTGTGACA CCCGGGATCC ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT
