NM_001792.5;ACOGS; ARVD14; CD325; CDHN; CDw325; NCAD
100μg liquid plasmid(Instantly):$105
| name | pCMV-mCherry-CDH2(human)-Neo | another name | apCMV-mCherry-CDH2(human)-Neo |
| Insert Size (bp) | 2721bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCTCGAGC TGGAGGTGGA GGTAGTGGAG GAGGCGGAAG CAAGCTTATG TGCCGGATAG CGGGAGCGCT GCGGACCCTG CTGCCGCTGC TGGCGGCCCT GCTTCAGGCG TCTGTAGAGG CTTCTGGTGA AATCGCATTA TGCAAGACTG GATTTCCTGA AGATGTTTAC AGTGCAGTCT TATCGAAGGA TGTGCATGAA GGACAGCCTC TTCTCAATGT GAAGTTTAGC AACTGCAATG GAAAAAGAAA AGTACAATAT GAGAGCAGTG AGCCTGCAGA TTTTAAGGTG GATGAAGATG GCATGGTGTA TGCCGTGAGA AGCTTTCCAC TCTCTTCTGA GCATGCCAAG TTCCTGATAT ATGCCCAAGA CAAAGAGACC CAGGAAAAGT GGCAAGTGGC AGTAAAATTG AGCCTGAAGC CAACCTTAAC TGAGGAGTCA GTGAAGGAGT CAGCAGAAGT TGAAGAAATA GTGTTCCCAA GACAATTCAG TAAGCACAGT GGCCACCTAC AAAGGCAGAA GAGAGACTGG GTCATCCCTC CAATCAACTT GCCAGAAAAC TCCAGGGGAC CTTTTCCTCA AGAGCTTGTC AGGATCAGGT CTGATAGAGA TAAAAACCTT TCACTGCGGT ACAGTGTAAC TGGGCCAGGA GCTGACCAGC CTCCAACTGG TATCTTCATT ATCAACCCCA TCTCGGGTCA GCTGTCGGTG ACAAAGCCCC TGGATCGCGA GCAGATAGCC CGGTTTCATT TGAGGGCACA TGCAGTAGAT ATTAATGGAA ATCAAGTGGA GAACCCCATT GACATTGTCA TCAATGTTAT TGACATGAAT GACAACAGAC CTGAGTTCTT ACACCAGGTT TGGAATGGGA CAGTTCCTGA GGGATCAAAG CCTGGAACAT ATGTGATGAC CGTAACAGCA ATTGATGCTG ACGATCCCAA TGCCCTCAAT GGGATGTTGA GGTACAGAAT CGTGTCTCAG GCTCCAAGCA CCCCTTCACC CAACATGTTT ACAATCAACA ATGAGACTGG TGACATCATC ACAGTGGCAG CTGGACTTGA TCGAGAAAAA GTGCAACAGT ATACGTTAAT AATTCAAGCT ACAGACATGG AAGGCAATCC CACATATGGC CTTTCAAACA CAGCCACGGC CGTCATCACA GTGACAGATG TCAATGACAA TCCTCCAGAG TTTACTGCCA TGACGTTTTA TGGTGAAGTT CCTGAGAACA GGGTAGACAT CATAGTAGCT AATCTAACTG TGACCGATAA GGATCAACCC CATACACCAG CCTGGAACGC AGTGTACAGA ATCAGTGGCG GAGATCCTAC TGGACGGTTC GCCATCCAGA CCGACCCAAA CAGCAACGAC GGGTTAGTCA CCGTGGTCAA ACCAATCGAC TTTGAAACAA ATAGGATGTT TGTCCTTACT GTTGCTGCAG AAAATCAAGT GCCATTAGCC AAGGGAATTC AGCACCCCCC TCAGTCAACT GCAACCGTGT CTGTTACAGT TATTGACGTA AATGAAAACC CTTATTTTGC CCCCAATCCT AAGATCATTC GCCAAGAAGA AGGGCTTCAT GCCGGTACCA TGTTGACAAC ATTCACTGCT CAGGACCCAG ATCGATATAT GCAGCAAAAT ATTAGATACA CTAAATTATC TGATCCTGCC AATTGGCTAA AAATAGATCC TGTGAATGGA CAAATAACTA CAATTGCTGT TTTGGACCGA GAATCACCAA ATGTGAAAAA CAATATATAT AATGCTACTT TCCTTGCTTC TGACAATGGA ATTCCTCCTA TGAGTGGAAC AGGAACGCTG CAGATCTATT TACTTGATAT TAATGACAAT GCCCCTCAAG TGTTACCTCA AGAGGCAGAG ACTTGCGAAA CTCCAGACCC CAATTCAATT AATATTACAG CACTTGATTA TGACATTGAT CCAAATGCTG GACCATTTGC TTTTGATCTT CCTTTATCTC CAGTGACTAT TAAGAGAAAT TGGACCATCA CTCGGCTTAA TGGTGATTTT GCTCAGCTTA ATTTAAAGAT AAAATTTCTT GAAGCTGGTA TCTATGAAGT TCCCATCATA ATCACAGATT CGGGTAATCC TCCCAAATCA AATATTTCCA TCCTGCGTGT GAAGGTTTGC CAGTGTGACT CCAACGGGGA CTGCACAGAT GTGGACAGGA TTGTGGGTGC GGGGCTTGGC ACCGGTGCCA TCATTGCCAT CCTGCTCTGC ATCATCATCC TGCTTATCCT TGTGCTGATG TTTGTGGTAT GGATGAAACG CCGGGATAAA GAACGCCAGG CCAAACAACT TTTAATTGAT CCAGAAGATG ATGTAAGAGA TAATATTTTA AAATATGATG AAGAAGGTGG AGGAGAAGAA GACCAGGACT ATGACTTGAG CCAGCTGCAG CAGCCTGACA CTGTGGAGCC TGATGCCATC AAGCCTGTGG GAATCCGACG AATGGATGAA AGACCCATCC ACGCCGAGCC CCAGTATCCG GTCCGATCTG CAGCCCCACA CCCTGGAGAC ATTGGGGACT TCATTAATGA GGGCCTTAAA GCGGCTGACA ATGACCCCAC AGCTCCACCA TATGACTCCC TGTTAGTGTT TGACTATGAA GGCAGTGGCT CCACTGCTGG GTCCTTGAGC TCCCTTAATT CCTCAAGTAG TGGTGGTGAG CAGGACTATG ATTACCTGAA CGACTGGGGG CCACGGTTCA AGAAACTTGC TGACATGTAT GGTGGAGGTG ATGACTGAGG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG GTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCATGCAT
