NM_001405509.1;AI326842; Ddx9; HEL-5; mHEL-5; NDHII; RHA
100μg liquid plasmid(Instantly):$163
| name | pAAV-hSyn-EGFP-DHX9(mouse) | another name | apAAV-hSyn-EGFP-DHX9(mouse) |
| Insert Size (bp) | 4155bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | hSyn | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGTCCTGC AGGCAGCTGC GCGCTCGCTC GCTCACTGAG GCCGCCCGGG CGTCGGGCGA CCTTTGGTCG CCCGGCCTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG TTCCTGCGGC CGCACGCGTG TGTCTAGACT GCAGAGGGCC CTGCGTATGA GTGCAAGTGG GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT CGTGCCTGAG AGCGCAGTCG AGAAGGTACC GGATCCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GAGGTGGCGG GAGCGGCGGT GGAGGTAGTG AATTCATGGG TGACATTAAA AATTTTCTGT ATGCCTGGTG TGGCAAAAGG AAGATGACTC CAGCCTATGA AATTAGAGCA GTAGGAAACA AAAACAGACA GAAATTCATG TGTGAGGTTC GAGTAGAAGG GTTTAATTAT GCTGGTATGG GCAATTCCAC CAATAAAAAG GATGCACAGA GCAATGCTGC CAGAGACTTT GTTAACTATT TGGTTCGAAT AAATGAAGTA AAGAGTGAAG AAGTTCCAGC TGTTGGGATT GTACCACCCC CACCCATACT TAGCGACACC TCTGATAGCA CAGCAAGTGC TGCAGAAGGG TTACCAGCAC CAATGGGAGG ACCACTTCCT CCTCATCTGG CTCTCAAAGC AGAAGCAGAA AATAATTCTG GAGTTGAGTC TTCTGGCTAT GGCTCTCCTG GACCTACTTG GGACCGAGGA GCCAACCTTA AAGATTACTA CTCAAGAAAG GAAGAACAAG AAGTACAAGC GACTCTAGAG TCAGAAGAAG TGGATTTAAA TGCTGGGCTT CATGGAAATT GGACACTGGA AAATGCTAAG GCTCGTCTGA ACCAGTATTT CCAAAAAGAA AAGATCCAAG GAGAATATAA GTACACCCAA GTGGGTCCCG ACCACAACAG GAGCTTTATT GCAGAAATGA CCATTTATAT CAAGCAGCTT GGCAGAAGGA TTTTCGCACG TGAACATGGA TCTAATAAGA AATTAGCAGC ACAGTCATGT GCCCTCTCAC TTGTCCGACA GCTCTATCAT CTTGGAGTGA TTGAGGCTTA CTCTGGGCTT ACAAAGAAGA AAGAAGGAGA GAGAGTGGAG CCTTACAAAG TTTTCCTTTC TCCAGATTTG GAGCTTCAGC TGCAAAATGT GGTTCAAGAG CTAGATCTTG AAATTGTGCC CCCACCTGTT GATCCTTCTA TGCCAGTTAT ACTTAACATT GGGAAATTGG CTCATTTTGA ACCATCTCAG AGACAAAATG CAGTGGGAGT GGTTCCTTGG TCACCTCCAC AATCCAACTG GAATCCTTGG ACAAGTAGCA ACATTGATGA GGGACCCCTT GCTTATGCTT CTACAGAACA AATAAGCATG GACCTTAAGA ATGAATTAAC ATACCAGATG GAACAAGATC ATAATTTACA AAGTGTGTTA CAAGAGAGAG AGTTGCTGCC TGTGAAGAAA TTTGAAGCTG AAATTCTGGA AGCCATTAGT TCAAATTCAG TTGTGATTAT CCGAGGGGCT ACTGGTTGTG GTAAAACCAC ACAAGTTCCA CAGTACATTT TGGATGACTT TATCCAGAAT GACAGAGCAG CCGAATGCAA TATTGTTGTG ACACAGCCCA GAAGGATCAG TGCAGTTGCA GTGGCAGAGC GAGTTGCTTA TGAGAGAGGA GAAGAGCCTG GAAAAAGCTG TGGTTACAGT GTTCGATTTG AGTCTATACT TCCACGCCCT CATGCCAGTA TAATGTTTTG TACTGTAGGT GTGCTTCTAA GAAAACTAGA AGCCGGCATT CGAGGAATCA GTCATGTTAT TGTGGATGAA ATTCATGAAA GAGATATTAA TACTGACTTC CTTTTGGTAG TACTGCGTGA TGTGGTTTTG GCTTATCCTG AAGTCCGTAT TGTTCTTATG TCTGCAACTA TTGATACCAC CATGTTCTGT GAATATTTCT TCAATTGCCC CATCATTGAG GTTTATGGAA GGACTTTCCC AGTTCAAGAG TATTTTCTGG AAGACTGCAT TCAGATGACT CAATTCATTC CTCCTCCAAA GGACAAGAAA AAGAAGGATA AGGAGGATGA TGGTGGTGAA GATGATGATG CAAATTGCAA CCTAATATGT GGTGACGAAT ATGGGCCAGA AACAAAGTTG AGCATGTCTC AGTTGAATGA AAAGGAAACT CCTTTTGAAC TTATTGAGGC GCTTCTTAAA TACATTGAAA CCCTTAATGT TCCTGGAGCT GTGTTGGTAT TTTTACCTGG CTGGAACTTG ATTTATACTA TGCAAAAGCA TTTAGAAAAT AATTCGCATT TCGGAAGTCA CCGGTATCAG ATTCTACCTC TCCATTCTCA GATTCCTCGT GAGGAACAGC GCAAAGTGTT TGATCCAGTA CCAGATGGAG TAACCAAGGT TATTTTGTCC ACAAACATTG CTGAAACAAG CATTACCATA AATGATGTTG TTTACGTCAT TGACTCCTGC AAACAAAAAG TGAAACTATT TACTGCTCAC AACAATATGA CCAACTATGC TACTGTATGG GCATCAAAAA CAAACCTTGA GCAGAGGAAA GGGCGAGCTG GCCGTGTACG ACCTGGGTTC TGTTTCCACC TTTGTAGTCG AGCTCGTTTT GACAGACTAG AGACCCACAT GACACCAGAG ATGTTTAGAA CACCATTGCA TGAAATTGCT CTGAGCATAA AACTTCTGCG TCTAGGAGGA ATTGGCCAAT TCCTTGCAAA GGCAATCGAA CCTCCACCTT TGGATGCTGT AATTGAAGCA GAGCATACTC TAAGAGAACT TGATGCTTTA GATGCCAATG ATGAGTTGAC TCCTCTTGGA CGAATCCTGG CTAAACTCCC CATTGAACCT CGTTTTGGAA AAATGATGAT AATGGGGTGT ATTTTCTATG TGGGAGATGC TGTCTGTACC ATCTCTGCTG CTACCTGCTT CCCAGAACCC TTTATCAGCG AAGGAAAACG CCTGGGATAC ATACACCGGA ATTTTGCTGG AAACAGATTT TCTGATCACG TGGCTCTTTT ATCAGTATTC CAAGCCTGGG ATGATGCTAG GATGAGTGGA GAAGAAGCAG AAATACGTTT TTGTGAGCAG AAGAGACTCA ATATGGCTAC ACTGAGAATG ACTTGGGAGG CCAAAGTACA GCTTAAAGAG ATTCTGATTA ACTCTGGATT TCCCGAAGAT TGTTTATTGA CACAAGTGTT TACTAACACT GGACCAGATA ATAATTTGGA TGTTGTTATC TCTCTGCTGG CTTTTGGTGT GTACCCCAAT GTATGCTATC ATAAGGAGAA GAGAAAGATT CTTACCACTG AAGGGCGCAA TGCACTTATC CACAAGTCAT CTGTGAACTG TCCTTTCAGC AGCCAAGACA TGAAGTACCC ATCTCCTTTC TTTGTTTTTG GTGAAAAGAT TAGAACCCGA GCCATCTCTG CTAAAGGCAT GACCTTAGTG ACCCCCTTAC AGCTACTTCT CTTTGCCTCC AAGAAAGTCC AGTCGGATGG GCAGATTGTG TTTATAGATG ACTGGATCAG ACTGCAAATA TCTCATGAAG CTGCAGCATG CATCACAGCC CTCCGGGCAG CCATGGAAGC TCTGGTTGTT GAAGTGTCCA AACAACCAAA TATTATCAGC CAATTGGACC CTGTAAATGA ACATATGCTA AACACAATCC GACAGATTTC TAGGCCCTCA GCTGCTGGTA TCAACCTTAT GATTGGAAGT GTAAGGTACG GCGATGGTCC ACGACCTCCC AAGATGGCCC GATATGATAA TGGAAGTGGA TATAGAAGGG GATATGGTGG GGGAGGATAT GGTGGCGGAG GATATGGTGG CGGATACGGT AGTGGAGGCT TTGGAGGAGG CTTTGGAAGT GGGGGAGGCT TTGGAGGAGG CTTTAACAGC GGTGGGGGAG GCTTTGGTAG TGGTGGGGGA GGCTTTGGCA GTGGTGGAGG TGGATTTGGT GGTGGTGGAG GAGGCTTCAG TGGTGGAGGA GGTGGTGGAT TTGGTGGTGG CAGAGGAGGT GGAGGTGGCG GATTTGGGGG TAGTGGGGGC TTTGGAAGTG GTGGGGGAGG GTATGGTGTT GGAGGTGGTG GCTATGGAGG AGGTGGTGGT GGTGGCTATG GAGGAGGCAG TGGAGGATAT GGAGGTGGGG GAGGGGGTTA TGGTGGTGGG GAAGGTTATA GTATAAGCCC CAACTCTTAC CGTGGGAATT ATGGTGGTGG CGGGGGAGGG TATAGAGGAG GTTCCCAAGG AGGCTACAGA AACAACTTTG GTGGAGACTA CAGAGGGTCC AGTGGAGACT ACCGGGGATC CGGTGGGGGC TATAGAGGAT CTGGAGGATT CCAGCGAAGG GGTTATGGAG GTGGTTATTT TGGACAAGGA AGAGGAGGAG GGGGTGGTGG TGGCTATTAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTATGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGA GCGCTGCTCG AGAGATCTAC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG TAACCACGTG CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCTT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACTCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGTCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCA
