p35S-bHLH112(rice)-EGFP-35S-Hyg
$84.00
In stock
SKU
PL45792
100μg liquid plasmid(Instantly):$105
| name | p35S-bHLH112(rice)-EGFP-35S-Hyg | another name | ap35S-bHLH112(rice)-EGFP-35S-Hyg |
| Insert Size (bp) | 1392bp | Plasmid host | Plant |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rice | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | Green |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTTGAGAC TTTTCAACAA AGGGTAATAT CCGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTTATTGTG AAGATAGTGG AAAAGGAAGG TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCC ATCGTTGAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGATATC TCCACTGACG TAAGGGATGA CGCACAATCC CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT GGAGAGAACA CGGGGGACTC TAGAGGATCC GAATTCATGG CAGATCACCA GGAGATGATC CACGCCGCGG CGGCGCCGGC CATGTACGGC GGCGGCGCGG CAGCGGGTGG TGCTACGACG ACGACGGGGA GCCACGGCGG CGGCGGCGGC GACTGGTGGA GCGCCGCGGT GTCGTCGTGC TCGGCGCCGG CGCCGGAGAC GATGCAAGGG TTCGGCGGCT GGTCGGCCGC GGTGGTGGCC GTCGACGGCG GTGGCAACAC GTCGAGGTCA GCTGCTGGTA ACACTGCTTC GTCGGAGTCG CCTGGATCGC TCGCCACCGG AAGCTCCATC ACTTTCCAGG AGCCCGCCGG CGGCGTCGCC GACCCGGCCG CCATCGCCGT GCACGCGCAG ACGGTGGCCG GCGGCGGCGG CGGCGGCTGG AATCAGCAGC CTTTCTTGGA TGGGAGTGGT TTCCATGGCT ACATGAGCTC ATCAAGAAAT GATCACCATA CCAATCATCA TCATCATCAG ATCAACACGC CGAGTTTGAT GAGCAACTCA TCATCAAATA ACGGCGTGAT GCTGCAAGAG CACCAACATG ACCAGAATTA CCAGTTCCTC TCCAACCTCG GCTTCGAGCT GCTCTCGTCG CCGACCTCGC CGTACGGCGG CGGTGGCGGC TTCCGCTCCT CGCTGCTCAG AAGCCTCACG GAGCCGGCGG CGGCAGCGAA GCCTAATGAC TCGCCGGGGT TTCAGCAGTA TCATCATCAT CAGCCGGCGA TGAACCTGCA GCCGCCGGCC GCCGCCGCCG GGAGAGAGCC ACTGCAGTTC ACCAACAGCA CGGCGGCGCC GTTTTGGAAC CCTTCCTCCA GGTTCACCGT CGCGGCGGAG GGTACAGCAC TAGGTGGTGC CGGAGCATCG CCGGCGCAGC CTACGCCGGC GAGCCTTGCA GCTAAGGTTA ATTGGCACAC ACTTAGGGCA TTAGAAGGAG TAGGAGACTC CAGCTCAATC ATCACGAAGA AGGCGAAGGC TGATTCCACG CCACTAAAGA AATCCAGAAC CGGGACGCCA TCGCCATTGC CGACGACCTT CAAGGTGAGG AAAGAGAAGC TAGGGGACAG GGTCACAGCA CTCCAACAAC TAGTCTCCCC TTTTGGAAAG ACGGACACGG CATCGGTGCT CCACGAGACA ATCGAGTACA TCAAGTTCCT GCACGATCAA GTTGGTGCAC TTAGTGCTCC TTACCTAAAG AACAGGCAAC AAGTGCCCCA CTTGAAGAAC TCCACCGGCG TCGACAACGA TGGCGGCGGC GGCGGCGGCG AGGCGACGGC GGCTAGCAAG AGAGACCTCA CCGGAAGAGG GCTGTGCCTG GTCCCGATAT CGAGCACCTT CGCCGTCGCC AGCGAGACGC CGGTCGATTT CTGGACGCCG TTCGGTGCAG CCTTCCGGAA GCTTATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCAC GGCATGGACG AGCTGTACAA GTAAAGCGGC CCGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGC TAGAGCAGCT TGAGCTTGGA TCAGATTGTC GTTTCCCGCC TTCAGTTTAA ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA GAATAACGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC TCGGGATCAA AGTACTTTGA TCCAACCCCT CCGCTGCTAT AGTGCAGTCG GCTTCTGACG TTCAGTGCAG CCGTCTTCTG AAAACGACAT GTCGCACAAG TCCTAAGTTA CGCGACAGGC TGCCGCCCTG CCCTTTTCCT GGCGTTTTCT TGTCGCGTGT TTTAGTCGCA TAAAGTAGAA TACTTGCGAC TAGAACCGGA GACATTACGC CATGAACAAG AGCGCCGCCG CTGGCCTGCT GGGCTATGCC CGCGTCAGCA CCGACGACCA GGACTTGACC AACCAACGGG CCGAACTGCA CGCGGCCGGC TGCACCAAGC TGTTTTCCGA GAAGATCACC GGCACCAGGC GCGACCGCCC GGAGCTGGCC AGGATGCTTG ACCACCTACG CCCTGGCGAC GTTGTGACAG TGACCAGGCT AGACCGCCTG GCCCGCAGCA CCCGCGACCT ACTGGACATT GCCGAGCGCA TCCAGGAGGC CGGCGCGGGC CTGCGTAGCC TGGCAGAGCC GTGGGCCGAC ACCACCACGC CGGCCGGCCG CATGGTGTTG ACCGTGTTCG CCGGCATTGC CGAGTTCGAG CGTTCCCTAA TCATCGACCG CACCCGGAGC GGGCGCGAGG CCGCCAAGGC CCGAGGCGTG AAGTTTGGCC CCCGCCCTAC CCTCACCCCG GCACAGATCG CGCACGCCCG CGAGCTGATC GACCAGGAAG GCCGCACCGT GAAAGAGGCG GCTGCACTGC TTGGCGTGCA TCGCTCGACC CTGTACCGCG CACTTGAGCG CAGCGAGGAA GTGACGCCCA CCGAGGCCAG GCGGCGCGGT GCCTTCCGTG AGGACGCATT GACCGAGGCC GACGCCCTGG CGGCCGCCGA GAATGAACGC CAAGAGGAAC AAGCATGAAA CCGCACCAGG ACGGCCAGGA CGAACCGTTT TTCATTACCG AAGAGATCGA GGCGGAGATG ATCGCGGCCG GGTACGTGTT CGAGCCGCCC GCGCACGTCT CAACCGTGCG GCTGCATGAA ATCCTGGCCG GTTTGTCTGA TGCCAAGCTG GCGGCCTGGC CGGCCAGCTT GGCCGCTGAA GAAACCGAGC GCCGCCGTCT AAAAAGGTGA TGTGTATTTG AGTAAAACAG CTTGCGTCAT GCGGTCGCTG CGTATATGAT GCGATGAGTA AATAAACAAA TACGCAAGGG GAACGCATGA AGGTTATCGC TGTACTTAAC CAGAAAGGCG GGTCAGGCAA GACGACCATC GCAACCCATC TAGCCCGCGC CCTGCAACTC GCCGGGGCCG ATGTTCTGTT AGTCGATTCC GATCCCCAGG GCAGTGCCCG CGATTGGGCG GCCGTGCGGG AAGATCAACC GCTAACCGTT GTCGGCATCG ACCGCCCGAC GATTGACCGC GACGTGAAGG CCATCGGCCG GCGCGACTTC GTAGTGATCG ACGGAGCGCC CCAGGCGGCG GACTTGGCTG TGTCCGCGAT CAAGGCAGCC GACTTCGTGC TGATTCCGGT GCAGCCAAGC CCTTACGACA TATGGGCCAC CGCCGACCTG GTGGAGCTGG TTAAGCAGCG CATTGAGGTC ACGGATGGAA GGCTACAAGC GGCCTTTGTC GTGTCGCGGG CGATCAAAGG CACGCGCATC GGCGGTGAGG TTGCCGAGGC GCTGGCCGGG TACGAGCTGC CCATTCTTGA GTCCCGTATC ACGCAGCGCG TGAGCTACCC AGGCACTGCC GCCGCCGGCA CAACCGTTCT TGAATCAGAA CCCGAGGGCG ACGCTGCCCG CGAGGTCCAG GCGCTGGCCG CTGAAATTAA ATCAAAACTC ATTTGAGTTA ATGAGGTAAA GAGAAAATGA GCAAAAGCAC AAACACGCTA AGTGCCGGCC GTCCGAGCGC ACGCAGCAGC AAGGCTGCAA CGTTGGCCAG CCTGGCAGAC ACGCCAGCCA TGAAGCGGGT CAACTTTCAG TTGCCGGCGG AGGATCACAC CAAGCTGAAG ATGTACGCGG TACGCCAAGG CAAGACCATT ACCGAGCTGC TATCTGAATA CATCGCGCAG CTACCAGAGT AAATGAGCAA ATGAATAAAT GAGTAGATGA ATTTTAGCGG CTAAAGGAGG CGGCATGGAA AATCAAGAAC AACCAGGCAC CGACGCCGTG GAATGCCCCA TGTGTGGAGG AACGGGCGGT TGGCCAGGCG TAAGCGGCTG GGTTGTCTGC CGGCCCTGCA ATGGCACTGG AACCCCCAAG CCCGAGGAAT CGGCGTGAGC GGTCGCAAAC CATCCGGCCC GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG AAGTTGAAGG CCGCGCAGGC CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT GAATCGTGGC AAGCGGCCGC TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC GGTGCGCCGT CGATTAGGAA GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG ATGCTCTATG ACGTGGGCAC CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT CTGTCGAAGC GTGACCGACG AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC GTAGAGGTTT CCGCAGGGCC GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG ATGGCGGTTT CCCATCTAAC CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG CCCGGCCGCG TGTTCCGTCC ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT GGCGGAAAGC AGAAAGACGA CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT GCCATGCAGC GTACGAAGAA GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA GCCTTGATTA GCCGCTACAA GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG ATCGAGCTAG CTGATTGGAT GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG ACGGTTCACC CCGATTACTT TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG GCACGCCGCG CCGCAGGCAA GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC AGTGGCAGCG CCGGAGAGTT CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA AATGACCTGC CGGAGTACGA TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC ATGCGCTACC GCAACCTGAT CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG ATGCTAGGGC AAATTGCCCT AGCAGGGGAA AAAGGTCGAA AAGGTCTCTT TCCTGTGGAT AGCACGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA GGCGATTTTT CCGCCTAAAA CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC TGTGCATAAC TGTCTGGCCA GCGCACAGCC GAAGAGCTGC AAAAAGCGCC TACCCTTCGG TCGCTGCGCT CCCTACGCCC CGCCGCTTCG CGTCGGCCTA TCGCGGCCGC TGGCCGCTCA AAAATGGCTG GCCTACGGCC AGGCAATCTA CCAGGGCGCG GACAAGCCGC GCCGTCGCCA CTCGACCGCC GGCGCCCACA TCAAGGCACC CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GCATTCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCG CGGTGATCAC AGGCAGCAAC GCTCTGTCAT CGTTACAATC AACATGCTAC CCTCCGCGAG ATCATCCGTG TTTCAAACCC GGCAGCTTAG TTGCCGTTCT TCCGAATAGC ATCGGTAACA TGAGCAAAGT CTGCCGCCTT ACAACGGCTC TCCCGCTGAC GCCGTCCCGG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGA ATTAACGCCG AATTAATTCG GGGGATCTGG ATTTTAGTAC TGGATTTTGG TTTTAGGAAT TAGAAATTTT ATTGATAGAA GTATTTTACA AATACAAATA CATACTAAGG GTTTCTTATA TGCTCAACAC ATGAGCGAAA CCCTATAGGA ACCCTAATTC CCTTATCTGG GAACTACTCA CACATTATTA TGGAGAAACT CGAGCTATTT CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGTCG TGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATAGCC TCCGCGACCG GTTGTAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TAAACTCCCC AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT CTCGAGAGAG ATAGATTTGT AGAGAGAGAC TGGTGATTTC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGTCTT GCGAAGGATA GTGGGATTGT GCGTCATCCC TTACGTCAGT GGAGATATCA CATCAATCCA CTTGCTTTGA AGACGTGGTT GGAACGTCTT CTTTTTCCAC GATGCTCCTC GTGGGTGGGG GTCCATCTTT GGGACCACTG TCGGCAGAGG CATCTTGAAC GATAGCCTTT CCTTTATCGC AATGATGGCA TTTGTAGGTG CCACCTTCCT TTTCTACTGT CCTTTTGATG AAGTGACAGA TAGCTGGGCA ATGGAATCCG AGGAGGTTTC CCGATATTAC CCTTTGTTGA AAAGTCTCAA TAGCCCTTTG GTCTTCTGAG ACTGTATCTT TGATATTCTT GGAGTAGACG AGAGTGTCGT GCTCCACCAT GTTATCACAT CAATCCACTT GCTTTGAAGA CGTGGTTGGA ACGTCTTCTT TTTCCACGAT GCTCCTCGTG GGTGGGGGTC CATCTTTGGG ACCACTGTCG GCAGAGGCAT CTTGAACGAT AGCCTTTCCT TTATCGCAAT GATGGCATTT GTAGGTGCCA CCTTCCTTTT CTACTGTCCT TTTGATGAAG TGACAGATAG CTGGGCAATG GAATCCGAGG AGGTTTCCCG ATATTACCCT TTGTTGAAAA GTCTCAATAG CCCTTTGGTC TTCTGAGACT GTATCTTTGA TATTCTTGGA GTAGACGAGA GTGTCGTGCT CCACCATGTT GGCAAGCTGC TCTAGCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC G
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