pCMV-RELN(human)-3×FLAG-Neo
$200.00
In stock
SKU
PL45784
NM_173054.3;ETL7; LIS2; PRO1598; RL
100μg liquid plasmid(Instantly):$221
| name | pCMV-RELN(human)-3×FLAG-Neo | another name | apCMV-RELN(human)-3×FLAG-Neo |
| Insert Size (bp) | 10375bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | BL21(DE3) | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGA GCGCAGTGGC TGGGCCCGGC AGACTTTCCT CCTAGCGCTG TTGCTGGGGG CGACGCTGAG GGCGCGCGCG GCGGCTGGCT ATTACCCCCG CTTTTCGCCC TTCTTTTTCC TGTGCACCCA CCACGGGGAG CTGGAAGGGG ATGGGGAGCA GGGCGAGGTG CTCATTTCCC TGCATATTGC GGGCAACCCC ACCTACTACG TTCCGGGACA AGAATACCAT GTGACAATTT CAACAAGCAC CTTTTTTGAC GGCTTGCTGG TGACAGGACT ATACACATCT ACAAGTGTTC AGGCATCACA GAGCATTGGA GGTTCCAGTG CTTTCGGATT TGGGATCATG TCTGACCACC AGTTTGGTAA CCAGTTTATG TGCAGTGTGG TAGCCTCTCA CGTGAGTCAC CTGCCCACAA CCAACCTCAG TTTCATCTGG ATTGCTCCAC CTGCGGGCAC AGGCTGTGTG AATTTCATGG CTACAGCAAC ACACCGGGGC CAGGTTATTT TCAAAGATGC TTTAGCCCAG CAGTTGTGTG AACAAGGAGC TCCAACAGAT GTCACTGTGC ACCCACATCT AGCTGAAATA CATAGTGACA GCATTATCCT GAGAGATGAC TTTGACTCCT ACCACCAACT GCAATTAAAT CCAAATATAT GGGTTGAATG TAACAACTGT GAGACTGGAG AACAGTGTGG CGCGATTATG CATGGCAATG CCGTCACCTT CTGTGAACCA TATGGCCCAC GAGAACTGAT TACCACAGGC CTTAATACAA CAACAGCTTC TGTCCTCCAA TTTTCCATTG GGTCAGGTTC ATGTCGCTTT AGTTATTCAG ACCCCAGCAT CATCGTGTTA TATGCCAAGA ATAACTCTGC GGACTGGATT CAGCTAGAGA AAATTAGAGC CCCTTCCAAT GTCAGCACAA TCATCCATAT CCTCTACCTT CCTGAGGACG CCAAAGGGGA GAATGTCCAA TTTCAGTGGA AGCAGGAAAA TCTTCGTGTA GGTGAAGTGT ATGAAGCCTG CTGGGCCTTA GATAACATCT TGATCATCAA TTCAGCTCAC AGACAAGTCG TTTTAGAAGA TAGTCTCGAC CCAGTGGACA CAGGCAACTG GCTTTTCTTC CCAGGAGCTA CAGTTAAGCA TAGCTGTCAG TCAGATGGGA ACTCCATTTA TTTCCATGGA AATGAAGGCA GCGAGTTCAA TTTTGCCACC ACCAGGGATG TAGATCTTTC CACAGAAGAT ATTCAAGAGC AATGGTCAGA AGAATTTGAG AGCCAGCCTA CAGGATGGGA TGTCTTGGGA GCTGTCATTG GTACAGAATG TGGAACGATA GAATCAGGCT TATCAATGGT CTTCCTCAAA GATGGAGAGA GGAAATTATG CACTCCATCC ATGGACACTA CCGGTTATGG GAACCTGAGG TTTTACTTTG TGATGGGAGG AATTTGTGAC CCTGGAAATT CTCATGAAAA TGACATAATC CTGTATGCAA AAATTGAAGG AAGAAAAGAG CATATAACAC TGGATACCCT TTCCTATTCC TCATATAAGG TTCCGTCTTT GGTTTCTGTG GTCATCAATC CTGAACTTCA GACTCCTGCT ACCAAATTTT GTCTCAGGCA AAAGAACCAT CAAGGACATA ATAGGAATGT CTGGGCTGTA GACTTTTTCC ATGTCTTGCC TGTTCTCCCT TCTACAATGT CTCACATGAT ACAGTTTTCC ATCAATCTGG GATGTGGAAC GCATCAGCCT GGTAACAGTG TCAGCTTGGA ATTTTCTACC AACCATGGGC GCTCCTGGTC CCTCCTTCAC ACTGAATGCT TACCTGAGAT CTGTGCTGGA CCCCACCTCC CCCACAGCAC TGTCTACTCC TCTGAAAACT ACAGTGGGTG GAACCGAATA ACAATTCCCC TTCCTAACGC AGCACTAACC CGGAACACCA GGATTCGCTG GAGACAAACA GGACCAATCC TTGGAAACAT GTGGGCAATT GATAATGTTT ATATTGGCCC GTCATGTCTC AAATTCTGTT CTGGCAGAGG ACAGTGCACT AGACATGGTT GCAAGTGTGA CCCTGGATTT TCTGGCCCAG CTTGTGAGAT GGCATCCCAG ACATTCCCAA TGTTTATTTC TGAAAGCTTT GGCAGTTCCA GGCTCTCCTC TTACCATAAC TTTTACTCTA TCCGTGGTGC TGAAGTCAGC TTTGGTTGTG GTGTCTTGGC CAGTGGTAAG GCCCTGGTTT TCAACAAAGA TGGGCGGCGT CAGCTAATTA CATCTTTCCT TGACAGCTCA CAATCCAGGT TTCTCCAGTT CACACTGAGA CTGGGGAGCA AATCTGTTCT GAGCACGTGC AGAGCCCCTG ATCAGCCTGG TGAAGGAGTT TTGTTGCATT ATTCTTATGA TAATGGGATA ACTTGGAAAC TCCTGGAGCA TTATTCATAT CTCAGCTATC ATGAGCCCAG AATAATCTCC GTAGAACTAC CAGGTGATGC AAAGCAGTTT GGAATTCAGT TCAGATGGTG GCAACCGTAT CATTCTTCCC AGAGAGAAGA TGTATGGGCT ATTGATGAGA TTATCATGAC ATCTGTGCTT TTCAACAGCA TTAGTCTTGA CTTTACCAAT CTTGTGGAGG TCACTCAGTC TCTGGGATTC TACCTTGGAA ATGTTCAGCC ATACTGTGGC CACGACTGGA CCCTTTGTTT TACAGGAGAT TCTAAACTTG CCTCAAGTAT GCGCTATGTG GAAACACAAT CAATGCAGAT AGGAGCATCC TATATGATTC AGTTCAGTTT GGTGATGGGA TGTGGCCAGA AATACACCCC ACACATGGAC AACCAGGTGA AGCTGGAGTA CTCAACCAAC CACGGCCTTA CCTGGCACCT CGTCCAAGAA GAATGCCTTC CAAGTATGCC AAGTTGTCAG GAATTTACAT CAGCAAGTAT TTACCATGCC AGTGAGTTTA CACAGTGGAG GAGAGTCATA GTGCTTCTTC CCCAGAAAAC TTGGTCCAGT GCTACCCGTT TCCGCTGGAG CCAGAGCTAT TACACAGCTC AAGACGAGTG GGCTTTGGAC AGCATTTACA TTGGGCAGCA GTGCCCCAAC ATGTGCAGTG GGCATGGCTC ATGCGATCAT GGCATATGCA GGTGTGACCA GGGGTACCAA GGCACTGAAT GCCACCCAGA AGCTGCCCTT CCGTCCACAA TTATGTCAGA TTTTGAGAAC CAGAATGGCT GGGAGTCTGA CTGGCAAGAA GTTATTGGGG GAGAAATTGT AAAACCAGAA CAAGGGTGTG GTGTCATCTC TTCTGGATCA TCTCTGTACT TCAGCAAGGC TGGGAAAAGA CAGCTGGTGA GTTGGGACCT GGATACTTCT TGGGTGGACT TTGTCCAGTT CTACATCCAG ATAGGCGGAG AGAGTGCTTC ATGCAACAAG CCTGACAGCA GAGAGGAGGG CGTCCTCCTT CAGTACAGCA ACAATGGGGG CATCCAGTGG CACCTGCTAG CAGAGATGTA CTTTTCAGAC TTCAGCAAAC CCAGATTTGT CTATCTGGAG CTTCCAGCTG CTGCCAAGAC CCCTTGCACC AGGTTCCGCT GGTGGCAGCC CGTGTTCTCA GGGGAGGACT ATGACCAGTG GGCAGTCGAT GACATCATCA TTCTGTCCGA GAAGCAGAAG CAGATCATCC CAGTTATCAA TCCAACTTTA CCTCAGAACT TTTATGAGAA GCCAGCTTTT GATTACCCTA TGAATCAGAT GAGTGTGTGG TTGATGTTGG CTAATGAAGG AATGGTTAAA AATGAAACCT TCTGTGCTGC CACACCATCA GCAATGATAT TTGGAAAATC AGATGGAGAT CGATTTGCAG TAACTCGAGA TTTGACCCTG AAACCTGGAT ATGTGCTACA GTTCAAGCTA AACATAGGTT GTGCCAATCA ATTCAGCAGT ACTGCTCCAG TTCTTCTTCA GTACTCTCAT GATGCTGGTA TGTCCTGGTT TCTGGTGAAA GAAGGCTGTT ACCCGGCTTC TGCAGGCAAA GGATGCGAAG GAAACTCCAG AGAACTAAGT GAGCCCACCA TGTATCACAC AGGGGACTTT GAAGAATGGA CAAGAATCAC CATTGTTATT CCAAGGTCTC TTGCATCCAG CAAGACCAGA TTCCGATGGA TCCAGGAGAG CAGCTCACAG AAAAACGTGC CTCCATTTGG TTTAGATGGA GTGTACATAT CCGAGCCTTG TCCCAGTTAC TGCAGTGGCC ATGGGGACTG CATTTCAGGA GTGTGTTTCT GTGACCTGGG ATATACTGCT GCACAAGGAA CCTGTGTGTC AAATGTCCCC AATCACAATG AGATGTTCGA TAGGTTTGAG GGGAAGCTCA GCCCTCTGTG GTACAAGATA ACAGGTGCCC AGGTTGGAAC TGGCTGTGGA ACACTTAACG ATGGCAAATC TCTCTACTTC AATGGCCCTG GGAAAAGGGA AGCCCGGACG GTCCCTCTGG ACACCAGGAA TATCAGACTT GTTCAATTTT ATATACAAAT TGGAAGCAAA ACTTCAGGCA TTACCTGCAT CAAACCAAGA ACTAGAAATG AAGGGCTTAT TGTTCAGTAT TCAAATGACA ATGGGATACT CTGGCATTTG CTTCGAGAGT TGGACTTCAT GTCCTTCCTG GAACCACAGA TCATTTCCAT TGACCTGCCA CAGGACGCGA AGACACCTGC AACGGCATTT CGATGGTGGC AACCGCAACA TGGGAAGCAT TCAGCCCAGT GGGCTTTGGA TGATGTTCTT ATAGGAATGA ATGACAGCTC TCAAACTGGA TTTCAAGACA AATTTGATGG CTCTATAGAT TTGCAAGCCA ACTGGTATCG AATCCAAGGA GGTCAAGTTG ATATTGACTG TCTCTCTATG GATACTGCTC TGATATTCAC TGAAAACATA GGAAAACCTC GTTATGCTGA GACCTGGGAT TTTCATGTGT CAGCATCTAC CTTTTTGCAG TTTGAAATGA GCATGGGCTG TAGCAAGCCC TTCAGCAACT CCCACAGTGT ACAGCTCCAG TATTCTCTGA ACAATGGCAA GGACTGGCAT CTTGTCACCG AAGAGTGTGT TCCTCCAACC ATTGGCTGTC TGCATTACAC GGAAAGTTCA ATTTACACCT CGGAAAGATT CCAGAATTGG AAGCGGATCA CTGTCTACCT TCCACTCTCC ACCATTTCTC CCAGGACCCG GTTCAGATGG ATTCAGGCCA ACTACACTGT GGGGGCTGAT TCCTGGGCGA TTGATAATGT TGTACTGGCC TCAGGGTGCC CTTGGATGTG CTCAGGACGA GGGATTTGTG ATGCTGGACG CTGTGTGTGT GACCGGGGCT TTGGTGGACC CTATTGTGTT CCTGTTGTTC CTCTGCCCTC GATTCTTAAA GACGATTTCA ATGGGAATTT ACATCCTGAC CTTTGGCCTG AAGTGTATGG TGCAGAGAGG GGGAATCTGA ATGGTGAAAC CATCAAATCT GGAACATCTC TAATTTTTAA AGGGGAAGGA CTAAGGATGC TTATTTCAAG AGATCTAGAT TGTACAAATA CAATGTATGT CCAGTTTTCA CTTAGATTTA TAGCAAAAAG TACCCCAGAG AGATCTCACT CTATTCTGTT ACAATTCTCC ATCAGTGGAG GAATCACTTG GCACCTGATG GATGAATTTT ACTTTCCTCA AACAACGAAT ATACTTTTCA TCAATGTTCC CTTGCCATAC ACTGCCCAAA CCAATGCTAC AAGATTCAGA CTCTGGCAAC CTTATAATAA CGGTAAGAAA GAAGAAATCT GGATTGTTGA TGACTTCATT ATCGATGGAA ATAATGTAAA CAACCCTGTG ATGCTCTTGG ATACATTTGA TTTTGGGCCC AGAGAAGACA ATTGGTTTTT CTATCCTGGT GGTAACATCG GTCTTTATTG TCCATATTCT TCAAAGGGGG CACCTGAAGA AGATTCAGCT ATGGTGTTTG TTTCAAATGA AGTTGGTGAG CATTCCATTA CCACCCGTGA CCTAAATGTG AATGAGAACA CCATCATACA ATTTGAGATC AACGTTGGCT GTTCGACTGA TAGCTCATCC GCGGATCCAG TGAGACTGGA ATTTTCAAGG GACTTCGGGG CGACCTGGCA CCTTCTGCTG CCCCTCTGCT ACCACAGCAG CAGCCACGTC AGCTCTTTAT GCTCCACCGA GCACCACCCC AGCAGCACCT ACTACGCAGG AACCATGCAG GGCTGGAGGA GGGAGGTCGT GCACTTTGGG AAGCTGCACC TTTGTGGATC TGTCCGTTTC AGATGGTACC AGGGATTTTA CCCTGCCGGC TCTCAGCCAG TGACATGGGC CATTGATAAT GTCTACATCG GTCCCCAGTG TGAGGAGATG TGTAATGGAC AGGGGAGCTG TATCAATGGA ACCAAATGTA TATGTGACCC TGGCTACTCA GGTCCAACCT GTAAAATAAG CACCAAAAAT CCTGATTTTC TCAAAGATGA TTTCGAAGGT CAGCTAGAAT CTGATAGATT CTTATTAATG AGTGGTGGGA AACCATCTCG AAAGTGTGGA ATCCTTTCTA GTGGAAACAA CCTCTTTTTC AATGAAGATG GCTTGCGCAT GTTGATGACA CGAGACCTGG ATTTATCACA TGCTAGATTT GTGCAGTTCT TCATGAGACT GGGATGTGGT AAAGGCGTTC CTGACCCCAG GAGTCAACCC GTGCTCCTAC AGTATTCTCT CAACGGTGGC CTCTCGTGGA GTCTTCTTCA GGAGTTCCTT TTCAGCAATT CCAGCAATGT GGGCAGGTAC ATTGCCCTGG AGATACCCTT GAAAGCCCGT TCTGGTTCTA CTCGCCTTCG CTGGTGGCAA CCGTCTGAGA ATGGGCACTT CTACAGCCCC TGGGTTATCG ATCAGATTCT TATTGGAGGA AATATTTCTG GTAATACGGT CTTGGAAGAT GATTTCACAA CCCTTGATAG TAGGAAATGG CTGCTTCACC CAGGAGGCAC CAAGATGCCC GTGTGTGGCT CTACTGGTGA TGCCCTGGTC TTCATTGAAA AGGCCAGCAC CCGTTACGTG GTCAGCACAG ACGTTGCCGT GAATGAGGAT TCCTTCCTAC AGATAGACTT CGCTGCCTCC TGCTCAGTCA CAGACTCTTG TTATGCGATT GAATTGGAAT ACTCAGTAGA TCTTGGATTG TCATGGCACC CATTGGTAAG GGACTGTCTG CCTACCAATG TGGAATGCAG TCGCTATCAT CTGCAACGGA TCCTGGTGTC AGACACTTTC AACAAGTGGA CTAGAATCAC TCTGCCTCTC CCTCCTTATA CCAGGTCCCA AGCCACTCGT TTCCGTTGGC ATCAACCAGC TCCTTTTGAC AAGCAGCAGA CATGGGCAAT AGATAATGTC TATATCGGGG ATGGCTGCAT AGACATGTGC AGTGGCCATG GGAGATGCAT CCAGGGAAAC TGCGTCTGTG ATGAACAGTG GGGTGGCCTG TACTGTGATG ACCCCGAGAC CTCTCTTCCA ACCCAACTCA AAGACAACTT CAATCGAGCT CCATCCAGTC AGAACTGGCT GACTGTGAAC GGAGGGAAAT TGAGTACAGT GTGTGGAGCC GTGGCGTCGG GAATGGCTCT CCATTTCAGT GGGGGTTGTA GTCGATTATT AGTCACTGTG GATCTAAACC TCACTAATGC TGAGTTCATC CAATTTTACT TCATGTATGG GTGCCTGATT ACACCAAACA ACCGTAACCA AGGTGTTCTC TTGGAATATT CTGTCAATGG AGGCATTACC TGGAACCTGC TCATGGAGAT TTTCTATGAC CAGTACAGTA AGCCCGGATT TGTGAATATC CTTCTCCCTC CTGATGCTAA AGAGATTGCC ACTCGCTTCC GCTGGTGGCA GCCAAGACAT GACGGCCTGG ATCAGAACGA CTGGGCCATT GACAATGTCC TCATCTCAGG CTCTGCTGAC CAAAGGACCG TTATGCTGGA CACCTTCAGC AGCGCCCCAG TACCCCAGCA TGAGCGCTCC CCTGCAGATG CCGGCCCTGT CGGGAGGATC GCCTTTGACA TGTTTATGGA AGACAAAACT TCAGTGAATG AGCACTGGCT ATTCCATGAT GATTGTACAG TAGAAAGATT CTGTGACTCC CCTGATGGTG TGATGCTCTG TGGCAGTCAT GATGGACGGG AGGTGTATGC AGTGACCCAT GACCTGACTC CCACTGAAGG CTGGATTATG CAATTCAAGA TCTCAGTTGG ATGTAAGGTG TCTGAAAAAA TTGCCCAGAA TCAAATTCAT GTGCAGTATT CTACTGACTT CGGTGTGAGT TGGAATTATC TGGTCCCTCA GTGCTTGCCT GCTGACCCAA AATGCTCTGG AAGTGTTTCT CAGCCATCTG TATTCTTTCC AACTAAAGGG TGGAAAAGGA TCACCTACCC ACTTCCTGAA AGCTTAGTGG GAAATCCGGT AAGGTTTAGG TTCTATCAGA AGTACTCAGA CATGCAGTGG GCAATCGATA ATTTCTACCT GGGCCCTGGA TGCTTGGACA ACTGCAGGGG CCATGGAGAT TGCTTAAGGG AACAGTGCAT CTGTGATCCG GGATACTCAG GGCCAAACTG CTACTTGACC CACACTCTGA AGACTTTCCT GAAGGAACGC TTTGACAGTG AAGAAATCAA ACCTGACTTA TGGATGTCCT TAGAAGGTGG AAGTACTTGC ACTGAGTGTG GAATTCTTGC CGAGGACACT GCACTCTATT TTGGGGGATC CACTGTGAGA CAAGCGGTTA CACAAGATTT GGATCTTCGA GGTGCAAAGT TCCTGCAATA CTGGGGGCGC ATCGGTAGTG AGAACAACAT GACCTCTTGC CATCGTCCCA TCTGCCGGAA GGAAGGCGTG CTGTTGGACT ACTCTACCGA TGGAGGAATT ACCTGGACTT TGCTCCATGA GATGGATTAC CAGAAATACA TTTCTGTTAG ACACGACTAC ATACTTCTTC CTGAAGATGC CCTCACCAAC ACAACTCGAC TTCGCTGGTG GCAGCCTTTT GTGATCAGCA ATGGAATTGT GGTCTCTGGG GTGGAGCGTG CTCAGTGGGC ACTGGACAAC ATTTTGATTG GTGGAGCAGA AATCAATCCC AGCCAATTGG TGGACACTTT TGATGATGAA GGCACTTCCC ATGAAGAAAA CTGGAGTTTT TACCCTAATG CTGTAAGGAC AGCAGGATTT TGTGGCAATC CATCCTTTCA CCTCTATTGG CCAAATAAAA AGAAGGACAA GACTCACAAT GCTCTCTCCT CCCGAGAACT CATTATACAG CCAGGATACA TGATGCAGTT TAAAATTGTG GTGGGTTGTG AAGCCACTTC TTGTGGTGAC CTTCATTCCG TAATGCTGGA ATACACTAAG GATGCAAGAT CGGATTCCTG GCAGCTCGTA CAGACCCAGT GCCTTCCTTC CTCTTCTAAC AGCATTGGCT GCTCCCCTTT CCAGTTCCAT GAAGCCACCA TCTACAACTC TGTCAACAGC TCAAGCTGGA AAAGAATCAC CATCCAGCTG CCTGACCATG TCTCCTCTAG TGCAACACAG TTCCGCTGGA TCCAGAAGGG AGAAGAAACT GAGAAGCAAA GCTGGGCAAT TGACCACGTG TACATTGGAG AGGCTTGCCC CAAGCTCTGC AGCGGGCACG GATACTGCAC GACCGGTGCC ATCTGCATCT GCGACGAGAG CTTCCAAGGT GATGACTGCT CTGTTTTCAG TCACGACCTT CCCAGTTATA TTAAAGATAA TTTTGAGTCC GCAAGAGTCA CCGAGGCAAA CTGGGAGACC ATTCAAGGTG GAGTCATAGG AAGTGGCTGT GGGCAGCTGG CCCCCTACGC CCATGGAGAC TCACTGTACT TTAATGGCTG TCAGATCAGG CAAGCAGCTA CCAAGCCTCT GGATCTCACT CGAGCAAGCA AAATCATGTT TGTTTTGCAA ATTGGGAGCA TGTCGCAGAC GGACAGCTGC AACAGTGACC TGAGTGGCCC CCACGCTGTG GACAAGGCAG TGCTGCTGCA ATACAGCGTC AACAACGGGA TCACCTGGCA TGTCATCGCC CAGCACCAGC CAAAGGACTT CACACAAGCT CAGAGAGTGT CTTACAATGT CCCCCTGGAG GCACGGATGA AAGGAGTCTT ACTGCGCTGG TGGCAACCAC GCCACAATGG AACAGGTCAT GATCAATGGG CTTTGGACCA TGTGGAGGTC GTCCTCACTC GCAAACAAAA TTACATGATG AATTTTTCAC GACAACATGG GCTCAGACAT TTCTACAACA GAAGACGAAG GTCACTTAGG CGATACCCAG GATCCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
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