NM_001174096.2;AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3;TCF8; ZFHEP; ZFHX1A
100μg liquid plasmid(Instantly):$120
| name | pCMV-ZEB1(human)-3×FLAG-Zeo | another name | apCMV-ZEB1(human)-3×FLAG-Zeo |
| Insert Size (bp) | 3378bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACGG GCCCTCTAGA GCCACCATGG CGGATGGCCC CAGGTGTAAG CGCAGAAAGC AGGCGAACCC GCGGCGCAAT AACGTTACAA ATTATAATAC TGTGGTAGAA ACAAATTCAG ATTCAGATGA TGAAGACAAA CTGCATATTG TGGAAGAAGA AAGTGTTACA GATGCAGCTG ACTGTGAAGG TGTACCAGAG GATGACCTGC CAACAGACCA GACAGTGTTA CCAGGGAGGA GCAGTGAAAG AGAAGGGAAT GCTAAGAACT GCTGGGAGGA TGACACAGGA AAGGAAGGGC AAGAAATCCT GGGGCCTGAA GCTCAGGCAG ATGAAGCAGG ATGTACAGTA AAAGATGATG AATGCGAGTC AGATGCAGAA AATGAGCAAA ACCATGATCC TAATGTTGAA GAGTTTCTAC AACAACAAGA CACTGCTGTC ATTTTTCCTG AGGCACCTGA AGAGGACCAG AGGCAGGGCA CACCAGAAGC CAGTGGTCAT GATGAAAATG GAACACCAGA TGCATTTTCA CAATTACTCA CCTGTCCATA TTGTGATAGA GGCTATAAAC GCTTTACCTC TCTGAAAGAA CACATTAAAT ATCGTCATGA AAAGAATGAA GATAACTTTA GTTGCTCCCT GTGCAGTTAC ACCTTTGCAT ACAGAACCCA ACTTGAACGT CACATGACAT CACATAAATC AGGAAGAGAT CAAAGACATG TGACGCAGTC TGGGTGTAAT CGTAAATTCA AATGCACTGA GTGTGGAAAA GCTTTCAAAT ACAAACATCA CCTAAAAGAG CACTTAAGAA TTCACAGTGG AGAGAAGCCA TATGAATGCC CAAACTGCAA GAAACGCTTT TCCCATTCTG GCTCCTATAG CTCACACATA AGCAGTAAGA AATGTATCAG CTTGATACCT GTGAATGGGC GACCAAGAAC AGGACTCAAG ACATCTCAGT GTTCTTCACC GTCTCTTTCA GCATCACCAG GCAGTCCCAC ACGACCACAG ATACGGCAAA AGATAGAGAA TAAACCCCTT CAAGAACAAC TTTCTGTTAA CCAAATTAAA ACTGAACCTG TGGATTATGA ATTCAAACCC ATAGTGGTTG CTTCAGGAAT CAACTGTTCA ACCCCTTTAC AAAATGGGGT TTTCACTGGT GGTGGCCCAT TACAGGCAAC CAGTTCTCCT CAGGGCATGG TGCAAGCTGT TGTTCTGCCA ACAGTTGGTT TGGTGTCTCC CATAAGTATC AATTTAAGTG ATATTCAGAA TGTACTTAAA GTGGCGGTAG ATGGTAATGT AATAAGGCAA GTGTTGGAGA ATAATCAAGC CAATCTTGCA TCCAAAGAAC AAGAAACAAT CAATGCTTCA CCCATACAAC AAGGTGGCCA TTCTGTTATT TCAGCCATCA GTCTTCCTTT GGTTGATCAA GATGGAACAA CCAAAATTAT CATCAACTAC AGTCTTGAGC AGCCTAGCCA ACTTCAAGTT GTTCCTCAAA ATTTAAAAAA AGAAAATCCA GTCGCTACAA ACAGTTGTAA AAGTGAAAAG TTACCAGAAG ATCTTACTGT TAAGTCTGAG AAGGACAAAA GCTTTGAAGG GGGGGTGAAT GATAGCACTT GTCTTCTGTG TGATGATTGT CCAGGAGATA TTAATGCACT TCCAGAATTA AAGCACTATG ACCTAAAGCA GCCTACTCAG CCTCCTCCAC TCCCTGCAGC AGAAGCTGAG AAGCCTGAGT CCTCTGTTTC ATCAGCTACT GGAGATGGCA ATTTGTCTCC TAGTCAGCCA CCTTTAAAGA ACCTCTTGTC TCTCCTAAAA GCATATTATG CTTTGAATGC ACAACCAAGT GCAGAAGAGC TCTCAAAAAT TGCTGATTCA GTAAACCTAC CACTGGATGT AGTAAAAAAG TGGTTTGAAA AGATGCAAGC TGGACAGATT TCAGTGCAGT CTTCTGAACC ATCTTCTCCT GAACCAGGCA AAGTAAATAT CCCTGCCAAG AACAATGATC AGCCTCAATC TGCAAATGCA AATGAACCCC AGGACAGCAC AGTAAATCTA CAAAGTCCTT TGAAGATGAC TAACTCCCCA GTTTTACCAG TGGGATCAAC CACCAATGGT TCCAGAAGTA GTACACCATC CCCATCACCT CTAAACCTTT CCTCATCCAG AAATACACAG GGTTACTTGT ACACAGCTGA GGGTGCACAA GAAGAGCCAC AAGTAGAACC TCTTGATCTT TCACTACCAA AGCAACAGGG AGAATTATTA GAAAGGTCAA CTATCACTAG TGTTTACCAG AACAGTGTTT ATTCTGTCCA GGAAGAACCC TTGAACTTGT CTTGCGCAAA AAAGGAGCCA CAAAAGGACA GTTGTGTTAC AGACTCAGAA CCAGTTGTAA ATGTAATCCC ACCAAGTGCC AACCCCATAA ATATCGCTAT ACCTACAGTC ACTGCCCAGT TACCCACAAT CGTGGCCATT GCTGACCAGA ACAGTGTTCC ATGCTTAAGA GCGCTAGCTG CCAATAAGCA AACGATTCTG ATTCCCCAGG TGGCATACAC CTACTCAACT ACGGTCAGCC CTGCAGTCCA AGAACCACCC TTGAAAGTGA TCCAGCCAAA TGGAAATCAG GATGAAAGAC AAGATACTAG CTCAGAAGGA GTATCAAATG TAGAGGATCA GAATGACTCT GATTCTACAC CGCCCAAAAA GAAAATGCGG AAGACAGAAA ATGGAATGTA TGCTTGTGAT TTGTGTGACA AGATATTCCA AAAGAGTAGT TCATTATTGA GACATAAATA TGAACACACA GGTAAAAGAC CTCATGAGTG TGGAATCTGT AAAAAGGCAT TTAAACACAA ACATCATTTG ATTGAACACA TGCGATTACA TTCTGGAGAA AAGCCCTATC AATGTGACAA ATGTGGAAAG CGCTTCTCAC ACTCTGGGTC TTATTCTCAA CACATGAATC ATCGCTACTC CTACTGTAAG AGAGAAGCGG AAGAACGTGA CAGCACAGAG CAGGAAGAGG CAGGGCCTGA AATCCTCTCG AATGAGCACG TGGGTGCCAG GGCGTCTCCC TCACAGGGCG ACTCGGACGA GAGAGAGAGT TTGACAAGGG AAGAGGATGA AGACAGTGAA AAAGAGGAAG AGGAGGAGGA TAAAGAGATG GAAGAATTGC AGGAAGAAAA AGAATGTGAA AAACCACAAG GGGATGAGGA AGAGGAGGAG GAGGAGGAAG AAGTGGAAGA AGAAGAGGTA GAAGAGGCAG AGAATGAGGG AGAAGAAGCA AAAACTGAAG GTCTGATGAA GGATGACAGG GCTGAAAGTC AAGCAAGCAG CTTAGGACAA AAAGTAGGCG AGAGTAGTGA GCAAGTGTCT GAAGAAAAGA CAAATGAAGC CCTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGGATC CGAGCTCGGT ACCAAGCTTA AGTTTAAACC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
