pCAMBIAGW-GUS
$113.00
In stock
SKU
PL45588
100μg liquid plasmid(Instantly):$156
| name | pCAMBIAGW-GUS | another name | apCAMBIAGW-GUS |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan,Chl |
| Selective marker | Hyg | Fluorescent labeling | Gus |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTGCGACATG TCGTTTTCAG AAGACGGCTG CACTGAACGT CAGAAGCCGA CTGCACTATA GCAGCGGAGG GGTTGGATCA AAGTACTTTG ATCCCGAGGG GAACCCTGTG GTTGGCATGC ACATACAAAT GGACGAACGG ATAAACCTTT TCACGCCCTT TTAAATATCC GATTATTCTA ATAAACGCTC TTTTCTCTTA GGTTTACCCG CCAATATATC CTGTCAAACA CTGATAGTTT AAACTGAAGG CGGGAAACGA CAATCTGATC CAAGCTCAAG CTGCTCTAGC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGAGCTGCTC TAGCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCACGGTATC GATAAGCTCG CGGATCCCTG AAAGCGACGT TGGATGTTAA CATCTACAAA TTGCCTTTTC TTATCGACCA TGTACGTAAG CGCTTACGTT TTTGGTGGAC CCTTGAGGAA ACTGGTAGCT GTTGTGGGCC TGTGGTCTCA AGATGGATCA TTAATTTCCA CCTTCACCTA CGATGGGGGG CATCGCACCG GTGAGTAATA TTGTACGGCT AAGAGCGAAT TTGGCCTGTA GGATCCCTGA AAGCGACGTT GGATGTTAAC ATCTACAAAT TGCCTTTTCT TATCGACCAT GTACGTAAGC GCTTACGTTT TTGGTGGACC CTTGAGGAAA CTGGTAGCTG TTGTGGGCCT GTGGTCTCAA GATGGATCAT TAATTTCCAC CTTCACCTAC GATGGGGGGC ATCGCACCGG TGAGTAATAT TGTACGGCTA AGAGCGAATT TGGCCTGTAG GATCCCTGAA AGCGACGTTG GATGTTAACA TCTACAAATT GCCTTTTCTT ATCGACCATG TACGTAAGCG CTTACGTTTT TGGTGGACCC TTGAGGAAAC TGGTAGCTGT TGTGGGCCTG TGGTCTCAAG ATGGATCATT AATTTCCACC TTCACCTACG ATGGGGGGCA TCGCACCGGT GAGTAATATT GTACGGCTAA GAGCGAATTT GGCCTGTAGG ATCCGCGAGC TGGTCAATCC CATTGCTTTT GAAGCAGCTC AACATTGATC TCTTTCTCGA TCGAGGGAGA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACAACT CTAGAAAGCT TCTGCAGGGG CCCGGGGTCG ACACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG GCCGCGGGTG ATGCTGCCAA CTTAGCGGCC GCTAAGTTGG CAGCATCACC CGACGCACTT TGCGCCGAAT AAATACCTGT GACGGAAGAT CACTTCGCAG AATAAATAAA TCCTGGTGTC CCTGTTGATA CCGGGAAGCC CTGGGCCAAC TTTTGGCGAA AATGAGACGT TGATCGGCAC GTAAGAGGTT CCAACTTTCA CCATAATGAA ATAAGATCAC TACCGGGCGT ATTTTTTGAG TCATCGAGAT TTTCAGGAGC TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA AGGGTTTATT GAGAATATGT TTTTCGTCTC AGCCAATCCC TGGGTGAGTT TCACCAGTTT TGATTTAAAC GTGGCCAATA TGGACAACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA TTATACGCAA GGCGACAAGG TGCTGATGCC GCTGGCGATT CAGGTTCATC ATGCCGTTTG TGATGGCTTC CATGTCGGCA GAATGCTTAA TGAATTACAA CAGTACTGCG ATGAGTGGCA GGCGGGGCGT AATCTAGAGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CCCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGGGTACCA TGGTAGATCT GACTAGTTTA CGTCCTGTAG AAACCCCAAC CCGTGAAATC AAAAAACTCG ACGGCCTGTG GGCATTCAGT CTGGATCGCG AAAACTGTGG AATTGATCAG CGTTGGTGGG AAAGCGCGTT ACAAGAAAGC CGGGCAATTG CTGTGCCAGG CAGTTTTAAC GATCAGTTCG CCGATGCAGA TATTCGTAAT TATGCGGGCA ACGTCTGGTA TCAGCGCGAA GTCTTTATAC CGAAAGGTTG GGCAGGCCAG CGTATCGTGC TGCGTTTCGA TGCGGTCACT CATTACGGCA AAGTGTGGGT CAATAATCAG GAAGTGATGG AGCATCAGGG CGGCTATACG CCATTTGAAG CCGATGTCAC GCCGTATGTT ATTGCCGGGA AAAGTGTACG TATCACCGTT TGTGTGAACA ACGAACTGAA CTGGCAGACT ATCCCGCCGG GAATGGTGAT TACCGACGAA AACGGCAAGA AAAAGCAGTC TTACTTCCAT GATTTCTTTA ACTATGCCGG AATCCATCGC AGCGTAATGC TCTACACCAC GCCGAACACC TGGGTGGACG ATATCACCGT GGTGACGCAT GTCGCGCAAG ACTGTAACCA CGCGTCTGTT GACTGGCAGG TGGTGGCCAA TGGTGATGTC AGCGTTGAAC TGCGTGATGC GGATCAACAG GTGGTTGCAA CTGGACAAGG CACTAGCGGG ACTTTGCAAG TGGTGAATCC GCACCTCTGG CAACCGGGTG AAGGTTATCT CTATGAACTG TGCGTCACAG CCAAAAGCCA GACAGAGTGT GATATCTACC CGCTTCGCGT CGGCATCCGG TCAGTGGCAG TGAAGGGCCA ACAGTTCCTG ATTAACCACA AACCGTTCTA CTTTACTGGC TTTGGTCGTC ATGAAGATGC GGACTTACGT GGCAAAGGAT TCGATAACGT GCTGATGGTG CACGACCACG CATTAATGGA CTGGATTGGG GCCAACTCCT ACCGTACCTC GCATTACCCT TACGCTGAAG AGATGCTCGA CTGGGCAGAT GAACATGGCA TCGTGGTGAT TGATGAAACT GCTGCTGTCG GCTTTCAGCT GTCTTTAGGC ATTGGTTTCG AAGCGGGCAA CAAGCCGAAA GAACTGTACA GCGAAGAGGC AGTCAACGGG GAAACTCAGC AAGCGCACTT ACAGGCGATT AAAGAGCTGA TAGCGCGTGA CAAAAACCAC CCAAGCGTGG TGATGTGGAG TATTGCCAAC GAACCGGATA CCCGTCCGCA AGGTGCACGG GAATATTTCG CGCCACTGGC GGAAGCAACG CGTAAACTCG ACCCGACGCG TCCGATCACC TGCGTCAATG TAATGTTCTG CGACGCTCAC ACCGATACCA TCAGCGATCT CTTTGATGTG CTGTGCCTGA ACCGTTATTA CGGATGGTAT GTCCAAAGCG GCGATTTGGA AACGGCAGAG AAGGTACTGG AAAAAGAACT TCTGGCCTGG CAGGAGAAAC TGCATCAGCC GATTATCATC ACCGAATACG GCGTGGATAC GTTAGCCGGG CTGCACTCAA TGTACACCGA CATGTGGAGT GAAGAGTATC AGTGTGCATG GCTGGATATG TATCACCGCG TCTTTGATCG CGTCAGCGCC GTCGTCGGTG AACAGGTATG GAATTTCGCC GATTTTGCGA CCTCGCAAGG CATATTGCGC GTTGGCGGTA ACAAGAAAGG GATCTTCACT CGCGACCGCA AACCGAAGTC GGCGGCTTTT CTGCTGCAAA AACGCTGGAC TGGCATGAAC TTCGGTGAAA AACCGCAGCA GGGAGGCAAA CAAGCTAGCT ACCCATACGA TGTTCCAGAT TACGCTTGAG CTCGAATTTC CCCGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGGGA ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGCT AGAGCAGCTT GCCAACATGG TGGAGCACGA CACTCTCGTC TACTCCAAGA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCTATTGA GACTTTTCAA CAAAGGGTAA TATCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTCATC AAAAGGACAG TAGAAAAGGA AGGTGGCACC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCGTTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACACGCTGAA ATCACCAGTC TCTCTCTACA AATCTATCTC TCTCGAGCTT TCGCAGATCC GGGGGGCAAT GAGATATGAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA GTTTAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTTCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT ACAACCGGTC GCGGAGGCTA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCACGACT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGAAATA GAGTAGATGC CGACCGGGAT CTGTCGATCG ACAAGCTCGA GTTTCTCCAT AATAATGTGT GAGTAGTTCC CAGATAAGGG AATTAGGGTT CCTATAGGGT TTCGCTCATG TGTTGAGCAT ATAAGAAACC CTTAGTATGT ATTTGTATTT GTAAAATACT TCTATCAATA AAATTTCTAA TTCCTAAAAC CAAAATCCAG TACTAAAATC CAGATCCCCC GAATTAATTC GGCGTTAATT CAGTACATTA AAAACGTCCG CAATGTGTTA TTAAGTTGTC TAAGCGTCAA TTTGTTTACA CCACAATATA TCCTGCCACC AGCCAGCCAA CAGCTCCCCG ACCGGCAGCT CGGCACAAAA TCACCACTCG ATACAGGCAG CCCATCAGTC CGGGACGGCG TCAGCGGGAG AGCCGTTGTA AGGCGGCAGA CTTTGCTCAT GTTACCGATG CTATTCGGAA GAACGGCAAC TAAGCTGCCG GGTTTGAAAC ACGGATGATC TCGCGGAGGG TAGCATGTTG ATTGTAACGA TGACAGAGCG TTGCTGCCTG TGATCACCGC GGTTTCAAAA TCGGCTCCGT CGATACTATG TTATACGCCA ACTTTGAAAA CAACTTTGAA AAAGCTGTTT TCTGGTATTT AAGGTTTTAG AATGCAAGGA ACAGTGAATT GGAGTTCGTC TTGTTATAAT TAGCTTCTTG GGGTATCTTT AAATACTGTA GAAAAGAGGA AGGAAATAAT AAATGGCTAA AATGAGAATA TCACCGGAAT TGAAAAAACT GATCGAAAAA TACCGCTGCG TAAAAGATAC GGAAGGAATG TCTCCTGCTA AGGTATATAA GCTGGTGGGA GAAAATGAAA ACCTATATTT AAAAATGACG GACAGCCGGT ATAAAGGGAC CACCTATGAT GTGGAACGGG AAAAGGACAT GATGCTATGG CTGGAAGGAA AGCTGCCTGT TCCAAAGGTC CTGCACTTTG AACGGCATGA TGGCTGGAGC AATCTGCTCA TGAGTGAGGC CGATGGCGTC CTTTGCTCGG AAGAGTATGA AGATGAACAA AGCCCTGAAA AGATTATCGA GCTGTATGCG GAGTGCATCA GGCTCTTTCA CTCCATCGAC ATATCGGATT GTCCCTATAC GAATAGCTTA GACAGCCGCT TAGCCGAATT GGATTACTTA CTGAATAACG ATCTGGCCGA TGTGGATTGC GAAAACTGGG AAGAAGACAC TCCATTTAAA GATCCGCGCG AGCTGTATGA TTTTTTAAAG ACGGAAAAGC CCGAAGAGGA ACTTGTCTTT TCCCACGGCG ACCTGGGAGA CAGCAACATC TTTGTGAAAG ATGGCAAAGT AAGTGGCTTT ATTGATCTTG GGAGAAGCGG CAGGGCGGAC AAGTGGTATG ACATTGCCTT CTGCGTCCGG TCGATCAGGG AGGATATCGG GGAAGAACAG TATGTCGAGC TATTTTTTGA CTTACTGGGG ATCAAGCCTG ATTGGGAGAA AATAAAATAT TATATTTTAC TGGATGAATT GTTTTAGTAC CTAGAATGCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGG GTGCCTTGAT GTGGGCGCCG GCGGTCGAGT GGCGACGGCG CGGCTTGTCC GCGCCCTGGT AGATTGCCTG GCCGTAGGCC AGCCATTTTT GAGCGGCCAG CGGCCGCGAT AGGCCGACGC GAAGCGGCGG GGCGTAGGGA GCGCAGCGAC CGAAGGGTAG GCGCTTTTTG CAGCTCTTCG GCTGTGCGCT GGCCAGACAG TTATGCACAG GCCAGGCGGG TTTTAAGAGT TTTAATAAGT TTTAAAGAGT TTTAGGCGGA AAAATCGCCT TTTTTCTCTT TTATATCAGT CACTTACATG TGTGACCGGT TCCCAATGTA CGGCTTTGGG TTCCCAATGT ACGGGTTCCG GTTCCCAATG TACGGCTTTG GGTTCCCAAT GTACGTGCTA TCCACAGGAA AGAGACCTTT TCGACCTTTT TCCCCTGCTA GGGCAATTTG CCCTAGCATC TGCTCCGTAC ATTAGGAACC GGCGGATGCT TCGCCCTCGA TCAGGTTGCG GTAGCGCATG ACTAGGATCG GGCCAGCCTG CCCCGCCTCC TCCTTCAAAT CGTACTCCGG CAGGTCATTT GACCCGATCA GCTTGCGCAC GGTGAAACAG AACTTCTTGA ACTCTCCGGC GCTGCCACTG CGTTCGTAGA TCGTCTTGAA CAACCATCTG GCTTCTGCCT TGCCTGCGGC GCGGCGTGCC AGGCGGTAGA GAAAACGGCC GATGCCGGGA TCGATCAAAA AGTAATCGGG GTGAACCGTC AGCACGTCCG GGTTCTTGCC TTCTGTGATC TCGCGGTACA TCCAATCAGC TAGCTCGATC TCGATGTACT CCGGCCGCCC GGTTTCGCTC TTTACGATCT TGTAGCGGCT AATCAAGGCT TCACCCTCGG ATACCGTCAC CAGGCGGCCG TTCTTGGCCT TCTTCGTACG CTGCATGGCA ACGTGCGTGG TGTTTAACCG AATGCAGGTT TCTACCAGGT CGTCTTTCTG CTTTCCGCCA TCGGCTCGCC GGCAGAACTT GAGTACGTCC GCAACGTGTG GACGGAACAC GCGGCCGGGC TTGTCTCCCT TCCCTTCCCG GTATCGGTTC ATGGATTCGG TTAGATGGGA AACCGCCATC AGTACCAGGT CGTAATCCCA CACACTGGCC ATGCCGGCCG GCCCTGCGGA AACCTCTACG TGCCCGTCTG GAAGCTCGTA GCGGATCACC TCGCCAGCTC GTCGGTCACG CTTCGACAGA CGGAAAACGG CCACGTCCAT GATGCTGCGA CTATCGCGGG TGCCCACGTC ATAGAGCATC GGAACGAAAA AATCTGGTTG CTCGTCGCCC TTGGGCGGCT TCATAATCGA CGGCGCACCG GCTGCCGGCG GTTGCCGGGA TTCTTTGCGG ATTCGATCAG CGGCCGCTTG CCACGATTCA CCGGGGCGTG CTTCTGCCTC GATGCGTTGC CGCTGGGCGG CCTGCGCGGC CTTCAACTTC TCCACCAGGT CATCACCCAG CGCCGCGCCG ATTTGTACCG GGCCGGATGG TTTGCGACCG CTCACGCCGA TTCCTCGGGC TTGGGGGTTC CAGTGCCATT GCAGGGCCGG CAGGCAACCC AGCCGCTTAC GCCTGGCCAA CCGCCCGTTC CTCCACACAT GGGGCATTCC ACGGCGTCGG TGCCTGGTTG TTCTTGATTT TCCATGCCGC CTCCTTTAGC CGCTAAAATT CATCTACTCA TTTATTCATT TGCTCATTTA CTCTGGTAGC TGCGCGATGT ATTCAGATAG CAGCTCGGTA ATGGTCTTGC CTTGGCGTAC CGCGTACATC TTCAGCTTGG TGTGATCCTC CGCCGGCAAC TGAAAGTTGA CCCGCTTCAT GGCTGGCGTG TCTGCCAGGC TGGCCAACGT TGCAGCCTTG CTGCTGCGTG CGCTCGGACG GCCGGCACTT AGCGTGTTTG TGCTTTTGCT CATTTTCTCT TTACCTCATT AACTCAAATG AGTTTTGATT TAATTTCAGC GGCCAGCGCC TGGACCTCGC GGGCAGCGTC GCCCTCGGGT TCTGATTCAA GAACGGTTGT GCCGGCGGCG GCAGTGCCTG GGTAGCTCAC GCGCTGCGTG ATACGGGACT CAAGAATGGG CAGCTCGTAC CCGGCCAGCG CCTCGGCAAC CTCACCGCCG ATGCGCGTGC CTTTGATCGC CCGCGACACG ACAAAGGCCG CTTGTAGCCT TCCATCCGTG ACCTCAATGC GCTGCTTAAC CAGCTCCACC AGGTCGGCGG TGGCCCATAT GTCGTAAGGG CTTGGCTGCA CCGGAATCAG CACGAAGTCG GCTGCCTTGA TCGCGGACAC AGCCAAGTCC GCCGCCTGGG GCGCTCCGTC GATCACTACG AAGTCGCGCC GGCCGATGGC CTTCACGTCG CGGTCAATCG TCGGGCGGTC GATGCCGACA ACGGTTAGCG GTTGATCTTC CCGCACGGCC GCCCAATCGC GGGCACTGCC CTGGGGATCG GAATCGACTA ACAGAACATC GGCCCCGGCG AGTTGCAGGG CGCGGGCTAG ATGGGTTGCG ATGGTCGTCT TGCCTGACCC GCCTTTCTGG TTAAGTACAG CGATAACCTT CATGCGTTCC CCTTGCGTAT TTGTTTATTT ACTCATCGCA TCATATACGC AGCGACCGCA TGACGCAAGC TGTTTTACTC AAATACACAT CACCTTTTTA GACGGCGGCG CTCGGTTTCT TCAGCGGCCA AGCTGGCCGG CCAGGCCGCC AGCTTGGCAT CAGACAAACC GGCCAGGATT TCATGCAGCC GCACGGTTGA GACGTGCGCG GGCGGCTCGA ACACGTACCC GGCCGCGATC ATCTCCGCCT CGATCTCTTC GGTAATGAAA AACGGTTCGT CCTGGCCGTC CTGGTGCGGT TTCATGCTTG TTCCTCTTGG CGTTCATTCT CGGCGGCCGC CAGGGCGTCG GCCTCGGTCA ATGCGTCCTC ACGGAAGGCA CCGCGCCGCC TGGCCTCGGT GGGCGTCACT TCCTCGCTGC GCTCAAGTGC GCGGTACAGG GTCGAGCGAT GCACGCCAAG CAGTGCAGCC GCCTCTTTCA CGGTGCGGCC TTCCTGGTCG ATCAGCTCGC GGGCGTGCGC GATCTGTGCC GGGGTGAGGG TAGGGCGGGG GCCAAACTTC ACGCCTCGGG CCTTGGCGGC CTCGCGCCCG CTCCGGGTGC GGTCGATGAT TAGGGAACGC TCGAACTCGG CAATGCCGGC GAACACGGTC AACACCATGC GGCCGGCCGG CGTGGTGGTG TCGGCCCACG GCTCTGCCAG GCTACGCAGG CCCGCGCCGG CCTCCTGGAT GCGCTCGGCA ATGTCCAGTA GGTCGCGGGT GCTGCGGGCC AGGCGGTCTA GCCTGGTCAC TGTCACAACG TCGCCAGGGC GTAGGTGGTC AAGCATCCTG GCCAGCTCCG GGCGGTCGCG CCTGGTGCCG GTGATCTTCT CGGAAAACAG CTTGGTGCAG CCGGCCGCGT GCAGTTCGGC CCGTTGGTTG GTCAAGTCCT GGTCGTCGGT GCTGACGCGG GCATAGCCCA GCAGGCCAGC GGCGGCGCTC TTGTTCATGG CGTAATGTCT CCGGTTCTAG TCGCAAGTAT TCTACTTTAT GCGACTAAAA CACGCGACAA GAAAACGCCA GGAAAAGGGC AGGGCGGCAG CCTGTCGCGT AACTTAGGAC TT
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