pSPECC1(human)-Fluc-TK-Rluc
$84.00
In stock
SKU
PL45585
100μg liquid plasmid(Instantly):$105
| name | pSPECC1(human)-Fluc-TK-Rluc | another name | apSPECC1(human)-Fluc-TK-Rluc |
| Insert Size (bp) | 1601bp | Plasmid host | Mammary cell |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCAACTG GCCGGTACCG GGCCTTGGAG GCCATTGTAA GGGCTTTGAC TTTCCCTCCA AGTGAGACAG AAAGACACTG GAGCGTTGCA AGTGGGGGTG TGACATGATT TGACCTAGGC TTTAGAAGCA TCACTCTCAC TGTATGTGCA GAATGGACTA TAAAGAGGCA AGGGCAGGAG AAGTGAGACT GGTTAGGAGG CAGTTGCAGT AATCCTGGCA GGAGATGACA GCGGCTTGGA TCGGGGGTAG CAGTGGAGGA GGTGAGAAGT GCTGAGATGT AGGGCTATTT ATGATACACA TACTGACAGG CGTTGTTGAG GGACGAGATG TGGCATGTGA AAGGAGTCGA GGTTTCTGGC CTGAGTCACT GGAAGAACGG AACTGCCACT TATGGAGATG GAGAGGACTA TTAAAAAACC TAAGTGGAGG TGTCAAGAGA AGGTGTCAAG AAGGTAGTTG GACAAATGAG TCTGGAGTTA GAAGAGGGAA CCAGCCTGGA GATATGTAGA TGGTATTTGA AAGCCACAAG TTGGTAAATC AAAGACCAAG AGAGTATATA GGAAAGAGGT CTGTTGGCTG AGCTTTAGTG CCCAGGGTGG GTCAAGATGA GGAGGAACCA ACAAAGGGGA CTGGGAAGAG GCTTCTAGGG GAGTGAGCAG AAAACTAGGA GAGGGTAGCA TCCTGGAACC AAGTGAACAC ACTGTTTGGA GGCGTTAGGA GTAATCAGTG GTGTCCTACG ATGTTGATGC GTGACACCTG AGAATGGACC GTAGTGTTCA AGTGATGCAC GAGATAGAAC CAGTCATACC ACTAATGGTT TTTTTAGTCC TAAATATTTT TACTGCAAGA TTTCGTTGTT CTAAGTGTTT TCACTGTCAG ATGAAGGATG AAAGTCGTTC AGGGTAATTT CCATTTTAGT AATTTCCGAC AGCGGCAGAA CAGTTTCTAC TTACGATTGG TATGCATTTT TATTATTAGC CATAGTATTC ATTTTGGGTG AGTACGTCTT TGTTAGCGTT ATTTTTCTTC ACCACCTCCT CGAGTTGTTC ATCCATTCAT TCATTCACCC AAAGAAATGC TTATTAAGCG CCTGTCCTGC GCTAGTCCTA GGTTTCTCTT GTCCTGATAG GTCTAGCTTC ACACGAGCAG GATTTTGCCT TTCCGGCGGC ACTAGGCTTC CGCCTCACCG CAGTGGGGTT TTTCCAGCCT ACCTTGTGCA GCTGCGGTTC TCAGCATCCG CGGGCGGAGG CCTGCAGCCG GTCAGCCGGT GCGTCCCGCC CACGGCGCGG GGCCTTCTGG CAGCCAAGAA GGAAATACAG ATGAGGTGGG CGGACAGTAT GCAGATGACG GGGGCGGGCC GCGCCTGGCG GGCGGTGTGC AGTTGAGGTG GTCCGGCAGG ATGCAGATGA AGGGGGCGGG CCGCGAGGGC GGGCTTGATG CAGATGAGGG GGCGGGGCCC ACGGGCGCGG GAGAGCAGCG GCTCCGCCGG CAGCTGCTGG GAACTGGAAG GCGCCCGGTC CTTTCTTTGA CTGGAGCGGA CCCGCCGGAC GCAACCGCCT CGCCAGCCGG AGCCAGCGCG AGCTCGGCAC GGTGGACACC CGGTCCGAGG CCGGCAAGCC CTCGAGGATA TCAAGATCTG GCCTCGGCGG CCAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGAAT TCTGCTTGCA AGAACTGGTT CAGTAGCTTA AGCCACTTTG TGATCCACCT TAACAGCCAC GGCTTCCCTC CCGAGGTGGA GGAGCAGGCC GCCGGCACCC TGCCCATGAG CTGCGCCCAG GAGAGCGGCA TGGATAGACA CCCTGCTGCT TGCGCCAGCG CCAGGATCAA CGTCTAACTG CAGTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTTAAATG AGTCTTCGGA CCTCGCGGGG GCCGCTTAAG CGGTGGTTAG GGTTTGTCTG ACGCGGGGGG AGGGGGAAGG AACGAAACAC TCTCATTCGG AGGCGGCTCG GGGTTTGGTC TTGGTGGCCA CGGGCACGCA GAAGAGCGCC GCGATCCTCT TAAGCACCCC CCCGCCCTCC GTGGAGGCGG GGGTTTGGTC GGCGGGTGGT AACTGGCGGG CCGCTGACTC GGGCGGGTCG CGCGCCCCAG AGTGTGACCT TTTCGGTCTG CTCGCAGACC CCCGGGCGGC GCCGCCGCGG CGGCGACGGG CTCGCTGGGT CCTAGGCTCC ATGGGGACCG TATACGTGGA CAGGCTCTGG AGCATCCGCA CGACTGCGGT GATATTACCG GAGACCTTCT GCGGGACGAG CCGGGTCACG CGGCTGACGC GGAGCGTCCG TTGGGCGACA AACACCAGGA CGGGGCACAG GTACACTATC TTGTCACCCG GAGGCGCGAG GGACTGCAGG AGCTTCAGGG AGTGGCGCAG CTGCTTCATC CCCGTGGCCC GTTGCTCGCG TTTGCTGGCG GTGTCCCCGG AAGAAATATA TTTGCATGTC TTTAGTTCTA TGATGACACA AACCCCGCCC AGCGTCTTGT CATTGGCGAA TTCGAACACG CAGATGCAGT CGGGGCGGCG CGGTCCCAGG TCCACTTCGC ATATTAAGGT GACGCGTGTG GCCTCGAACA CCGAGCGACC CTGCAGCGAC CCGCTTAAAA GCTTGATTCT TCTGACACAA CAGTCTCGAA CTTAAGCTGC AGAAGTTGGT CGTGAGGCAC TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA CTTTGCCTTT CTCTCCACAG GTGTCCACTC CCAGTTCAAT TACAGCTCTT AAGGCTAGAG TACTTAATAC GACTCACTAT AGGCTAGCCA CCATGACTTC GAAAGTTTAT GATCCAGAAC AAAGGAAACG GATGATAACT GGTCCGCAGT GGTGGGCCAG ATGTAAACAA ATGAATGTTC TTGATTCATT TATTAATTAT TATGATTCAG AAAAACATGC AGAAAATGCT GTTATTTTTT TACATGGTAA CGCGGCCTCT TCTTATTTAT GGCGACATGT TGTGCCACAT ATTGAGCCAG TAGCGCGGTG TATTATACCA GACCTTATTG GTATGGGCAA ATCAGGCAAA TCTGGTAATG GTTCTTATAG GTTACTTGAT CATTACAAAT ATCTTACTGC ATGGTTTGAA CTTCTTAATT TACCAAAGAA GATCATTTTT GTCGGCCATG ATTGGGGTGC TTGTTTGGCA TTTCATTATA GCTATGAGCA TCAAGATAAG ATCAAAGCAA TAGTTCACGC TGAAAGTGTA GTAGATGTGA TTGAATCATG GGATGAATGG CCTGATATTG AAGAAGATAT TGCGTTGATC AAATCTGAAG AAGGAGAAAA AATGGTTTTG GAGAATAACT TCTTCGTGGA AACCATGTTG CCATCAAAAA TCATGAGAAA GTTAGAACCA GAAGAATTTG CAGCATATCT TGAACCATTC AAAGAGAAAG GTGAAGTTCG TCGTCCAACA TTATCATGGC CTCGTGAAAT CCCGTTAGTA AAAGGTGGTA AACCTGACGT TGTACAAATT GTTAGGAATT ATAATGCTTA TCTACGTGCA AGTGATGATT TACCAAAAAT GTTTATTGAA TCGGACCCAG GATTCTTTTC CAATGCTATT GTTGAAGGTG CCAAGAAGTT TCCTAATACT GAATTTGTCA AAGTAAAAGG TCTTCATTTT TCGCAAGAAG ATGCACCTGA TGAAATGGGA AAATATATCA AATCGTTCGT TGAGCGAGTT CTCAAAAATG AACAATAATT CTAGAGCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGGCT CGAC
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