NM_004895.4;AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1;DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1
100μg liquid plasmid(Instantly):$120
| name | pCMV-NLRP3(human)-3×FLAG-Neo | another name | apCMV-NLRP3(human)-3×FLAG-Neo |
| Insert Size (bp) | 3111bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGAAGATGG CAAGCACCCG CTGCAAGCTG GCCAGGTACC TGGAGGACCT GGAGGATGTG GACTTGAAGA AATTTAAGAT GCACTTAGAG GACTATCCTC CCCAGAAGGG CTGCATCCCC CTCCCGAGGG GTCAGACAGA GAAGGCAGAC CATGTGGATC TAGCCACGCT AATGATCGAC TTCAATGGGG AGGAGAAGGC GTGGGCCATG GCCGTGTGGA TCTTCGCTGC GATCAACAGG AGAGACCTTT ATGAGAAAGC AAAAAGAGAT GAGCCGAAGT GGGGTTCAGA TAATGCACGT GTTTCGAATC CCACTGTGAT ATGCCAGGAA GACAGCATTG AAGAGGAGTG GATGGGTTTA CTGGAGTACC TTTCGAGAAT CTCTATTTGT AAAATGAAGA AAGATTACCG TAAGAAGTAC AGAAAGTACG TGAGAAGCAG ATTCCAGTGC ATTGAAGACA GGAATGCCCG TCTGGGTGAG AGTGTGAGCC TCAACAAACG CTACACACGA CTGCGTCTCA TCAAGGAGCA CCGGAGCCAG CAGGAGAGGG AGCAGGAGCT TCTGGCCATC GGCAAGACCA AGACGTGTGA GAGCCCCGTG AGTCCCATTA AGATGGAGTT GCTGTTTGAC CCCGATGATG AGCATTCTGA GCCTGTGCAC ACCGTGGTGT TCCAGGGGGC GGCAGGGATT GGGAAAACAA TCCTGGCCAG GAAGATGATG TTGGACTGGG CGTCGGGGAC ACTCTACCAA GACAGGTTTG ACTATCTGTT CTATATCCAC TGTCGGGAGG TGAGCCTTGT GACACAGAGG AGCCTGGGGG ACCTGATCAT GAGCTGCTGC CCCGACCCAA ACCCACCCAT CCACAAGATC GTGAGAAAAC CCTCCAGAAT CCTCTTCCTC ATGGACGGCT TCGATGAGCT GCAAGGTGCC TTTGACGAGC ACATAGGACC GCTCTGCACT GACTGGCAGA AGGCCGAGCG GGGAGACATT CTCCTGAGCA GCCTCATCAG AAAGAAGCTG CTTCCCGAGG CCTCTCTGCT CATCACCACG AGACCTGTGG CCCTGGAGAA ACTGCAGCAC TTGCTGGACC ATCCTCGGCA TGTGGAGATC CTGGGTTTCT CCGAGGCCAA AAGGAAAGAG TACTTCTTCA AGTACTTCTC TGATGAGGCC CAAGCCAGGG CAGCCTTCAG TCTGATTCAG GAGAACGAGG TCCTCTTCAC CATGTGCTTC ATCCCCCTGG TCTGCTGGAT CGTGTGCACT GGACTGAAAC AGCAGATGGA GAGTGGCAAG AGCCTTGCCC AGACATCCAA GACCACCACC GCGGTGTACG TCTTCTTCCT TTCCAGTTTG CTGCAGCCCC GGGGAGGGAG CCAGGAGCAC GGCCTCTGCG CCCACCTCTG GGGGCTCTGC TCTTTGGCTG CAGATGGAAT CTGGAACCAG AAAATCCTGT TTGAGGAGTC CGACCTCAGG AATCATGGAC TGCAGAAGGC GGATGTGTCT GCTTTCCTGA GGATGAACCT GTTCCAAAAG GAAGTGGACT GCGAGAAGTT CTACAGCTTC ATCCACATGA CTTTCCAGGA GTTCTTTGCC GCCATGTACT ACCTGCTGGA AGAGGAAAAG GAAGGAAGGA CGAACGTTCC AGGGAGTCGT TTGAAGCTTC CCAGCCGAGA CGTGACAGTC CTTCTGGAAA ACTATGGCAA ATTCGAAAAG GGGTATTTGA TTTTTGTTGT ACGTTTCCTC TTTGGCCTGG TAAACCAGGA GAGGACCTCC TACTTGGAGA AGAAATTAAG TTGCAAGATC TCTCAGCAAA TCAGGCTGGA GCTGCTGAAA TGGATTGAAG TGAAAGCCAA AGCTAAAAAG CTGCAGATCC AGCCCAGCCA GCTGGAATTG TTCTACTGTT TGTACGAGAT GCAGGAGGAG GACTTCGTGC AAAGGGCCAT GGACTATTTC CCCAAGATTG AGATCAATCT CTCCACCAGA ATGGACCACA TGGTTTCTTC CTTTTGCATT GAGAACTGTC ATCGGGTGGA GTCACTGTCC CTGGGGTTTC TCCATAACAT GCCCAAGGAG GAAGAGGAGG AGGAAAAGGA AGGCCGACAC CTTGATATGG TGCAGTGTGT CCTCCCAAGC TCCTCTCATG CTGCCTGTTC TCATGGATTG GTGAACAGCC ACCTCACTTC CAGTTTTTGC CGGGGCCTCT TTTCAGTTCT GAGCACCAGC CAGAGTCTAA CTGAATTGGA CCTCAGTGAC AATTCTCTGG GGGACCCAGG GATGAGAGTG TTGTGTGAAA CGCTCCAGCA TCCTGGCTGT AACATTCGGA GATTGTGGTT GGGGCGCTGT GGCCTCTCGC ATGAGTGCTG CTTCGACATC TCCTTGGTCC TCAGCAGCAA CCAGAAGCTG GTGGAGCTGG ACCTGAGTGA CAACGCCCTC GGTGACTTCG GAATCAGACT TCTGTGTGTG GGACTGAAGC ACCTGTTGTG CAATCTGAAG AAGCTCTGGT TGGTCAGCTG CTGCCTCACA TCAGCATGTT GTCAGGATCT TGCATCAGTA TTGAGCACCA GCCATTCCCT GACCAGACTC TATGTGGGGG AGAATGCCTT GGGAGACTCA GGAGTCGCAA TTTTATGTGA AAAAGCCAAG AATCCACAGT GTAACCTGCA GAAACTGGGG TTGGTGAATT CTGGCCTTAC GTCAGTCTGT TGTTCAGCTT TGTCCTCGGT ACTCAGCACT AATCAGAATC TCACGCACCT TTACCTGCGA GGCAACACTC TCGGAGACAA GGGGATCAAA CTACTCTGTG AGGGACTCTT GCACCCCGAC TGCAAGCTTC AGGTGTTGGA ATTAGACAAC TGCAACCTCA CGTCACACTG CTGCTGGGAT CTTTCCACAC TTCTGACCTC CAGCCAGAGC CTGCGAAAGC TGAGCCTGGG CAACAATGAC CTGGGCGACC TGGGGGTCAT GATGTTCTGT GAAGTGCTGA AACAGCAGAG CTGCCTCCTG CAGAACCTGG GGTTGTCTGA AATGTATTTC AATTATGAGA CAAAAAGTGC GTTAGAAACA CTTCAAGAAG AAAAGCCTGA GCTGACCGTC GTCTTTGAGC CTTCTTGGCT CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
