pNiFty2-SEAP
$99.00
In stock
SKU
PL45515
100μg liquid plasmid(Instantly):$120
| name | pNiFty2-SEAP | another name | apNiFty2-SEAP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Zeo | |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAATCAGCG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGT AACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCTATCG AAGGATCTGC GATCGCTGAA TTCTGGGGAC TTTCCACTGG GGACTTTCCA CTGGGGACTT TCCACTGGGG ACTTTCCACT GGGGACTTTC CACTCCTGCA GCAGTGGATA TTCCCAGAAA ACTTTTTGGA TGCAGTTGGG GATTTCCTCT TTACTGGATG TGGACAATAT CCTCCTATTA TTCACAGGAA GCAATCCCTC CTATAAAAGG GCCTCAGCAG AAGTAGTGTT CAGCTGTTCT TGGCTGACTT CACATCAAAG CTTCTATACT GACCTGAGAC AGAGCCATGG TTCTGGGGCC CTGCATGCTG CTGCTGCTGC TGCTGCTGGG CCTGAGGCTA CAGCTCTCCC TGGGCATCAT CCCAGTTGAG GAGGAGAACC CGGACTTCTG GAACCGCGAG GCAGCCGAGG CCCTGGGTGC CGCCAAGAAG CTGCAGCCTG CACAGACAGC CGCCAAGAAC CTCATCATCT TCCTGGGCGA TGGGATGGGG GTGTCTACGG TGACAGCTGC CAGGATCCTA AAAGGGCAGA AGAAGGACAA ACTGGGGCCT GAGATACCCC TGGCTATGGA CCGCTTCCCA TATGTGGCTC TGTCCAAGAC ATACAATGTA GACAAACATG TGCCAGACAG TGGAGCCACA GCCACGGCCT ACCTGTGCGG GGTCAAGGGC AACTTCCAGA CCATTGGCTT GAGTGCAGCC GCCCGCTTTA ACCAGTGCAA CACGACACGC GGCAACGAGG TCATCTCCGT GATGAATCGG GCCAAGAAAG CAGGGAAGTC AGTGGGAGTG GTAACCACCA CACGAGTGCA GCACGCCTCG CCAGCCGGCA CCTACGCCCA CACGGTGAAC CGCAACTGGT ACTCGGACGC CGACGTGCCT GCCTCGGCCC GCCAGGAGGG GTGCCAGGAC ATCGCTACGC AGCTCATCTC CAACATGGAC ATTGATGTGA TCCTGGGTGG AGGCCGAAAG TACATGTTTC GCATGGGAAC CCCAGACCCT GAGTACCCAG ATGACTACAG CCAAGGTGGG ACCAGGCTGG ACGGGAAGAA TCTGGTGCAG GAATGGCTGG CGAAGCGCCA GGGTGCCCGG TATGTGTGGA ACCGCACTGA GCTCATGCAG GCTTCCCTGG ACCCGTCTGT GACCCATCTC ATGGGTCTCT TTGAGCCTGG AGACATGAAA TACGAGATCC ACCGAGACTC CACACTGGAC CCCTCCCTGA TGGAGATGAC AGAGGCTGCC CTGCGCCTGC TGAGCAGGAA CCCCCGCGGC TTCTTCCTCT TCGTGGAGGG TGGTCGCATC GACCACGGTC ATCACGAAAG CAGGGCTTAC CGGGCACTGA CTGAGACGAT CATGTTCGAC GACGCCATTG AGAGGGCGGG CCAGCTCACC AGCGAGGAGG ACACGCTGAG CCTCGTCACT GCCGACCACT CCCACGTCTT CTCCTTCGGA GGCTACCCCC TGCGAGGGAG CTCCATCTTC GGGCTGGCCC CTGGCAAGGC CCGGGACAGG AAGGCCTACA CGGTCCTCCT ATACGGAAAC GGTCCAGGCT ATGTGCTCAA GGACGGCGCC CGGCCGGATG TTACCGAGAG CGAGAGCGGG AGCCCCGAGT ATCGGCAGCA GTCAGCAGTG CCCCTGGACG AAGAGACCCA CGCAGGCGAG GACGTGGCGG TGTTCGCGCG CGGCCCGCAG GCGCACCTGG TTCACGGCGT GCAGGAGCAG ACCTTCATAG CGCACGTCAT GGCCTTCGCC GCCTGCCTGG AGCCCTACAC CGCCTGCGAC CTGGCGCCCC CCGCCGGCAC CACCGACGCC GCGCACCCGG GGCGGTCCCG GTCCAAGCGT CTGGATTGAA GCTAGCTGGC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGAATTC TAAAATACAG CATAGCAAAA CTTTAACCTC CAAATCAAGC CTCTACTTGA ATCCTTTTCT GAGGGATGAA TAAGGCATAG GCATCAGGGG CTGTTGCCAA TGTGCATTAG CTGTTTGCAG CCTCACCTTC TTTCATGGAG TTTAAGATAT AGTGTATTTT CCCAAGGTTT GAACTAGCTC TTCATTTCTT TATGTTTTAA ATGCACTGAC CTCCCACATT CCCTTTTTAG TAAAATATTC AGAAATAATT TAAATACATC ATTGCAATGA AAATAAATGT TTTTTATTAG GCAGAATCCA GATGCTCAAG GCCCTTCATA ATATCCCCCA GTTTAGTAGT TGGACTTAGG GAACAAAGGA ACCTTTAATA GAAATTGGAC AGCAAGAAAG CGAGCTTCTA GCTTATCCTC AGTCCTGCTC CTCTGCCACA AAGTGCACGC AGTTGCCGGC CGGGTCGCGC AGGGCGAACT CCCGCCCCCA CGGCTGCTCG CCGATCTCGG TCATGGCCGG CCCGGAGGCG TCCCGGAAGT TCGTGGACAC GACCTCCGAC CACTCGGCGT ACAGCTCGTC CAGGCCGCGC ACCCACACCC AGGCCAGGGT GTTGTCCGGC ACCACCTGGT CCTGGACCGC GCTGATGAAC AGGGTCACGT CGTCCCGGAC CACACCGGCG AAGTCGTCCT CCACGAAGTC CCGGGAGAAC CCGAGCCGGT CGGTCCAGAA CTCGACCGCT CCGGCGACGT CGCGCGCGGT GAGCACCGGA ACGGCACTGG TCAACTTGGC CATGATGGCC CTCCTATAGT GAGTCGTATT ATACTATGCC GATATACTAT GCCGATGATT AATTGTCAAC TACTGTTTGT AGGCGCCGGT CACAGCTTGG ATCTGTAACG GCGCAGAACA GAAAACGAAA CAAAGACGTA GAGTTGAGCA AGCAGGGTCA GGCAAAGCGT GGAGAGCCGG CTGAGTCTAG GTAGGCTCCA AGGGAGCGCC GGACAAAGGC CCGGTCTCGA CCTGAGCTTT AAACTTACCT AGACGGCGGA CGCAGTTCAG GAGGCACCAC AGGCGGGAGG CGGCAGAACG CGACTCAACC GGCGTGGATG GCGGCCTCAG GTAGGGCGGC GGGCGCGTGA AGGAGAGATG CGAGCCCCTC GAAGCTTCAG CTGTGTTCTG GCGGCAAACC CGTTGCGAAA AAGAACGTTC ACGGCGACTA CTGCACTTAT ATACGGTTCT CCCCCACCCT CGGGAAAAAG GCGGAGCCAG TACACGACAT CACTTTCCCA GTTTACCCCG CGCCACCTTC TCTAGGCACC GGTTCAATTG CCGACCCCTC CCCCCAACTT CTCGGGGACT GTGGGCGATG TGCGCTCTGC CCACTGACTA GTGGGCCCTG CAGGTTAATT AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGG CTAGTTAATT AACATT
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