pCMV-TLR4(human)-SNAP-Neo
$84.00
In stock
SKU
PL45466
NM_138554.5;ARMD10; CD284; TLR-4; TOLL
100μg liquid plasmid(Instantly):$105
| name | pCMV-TLR4(human)-SNAP-Neo | another name | apCMV-TLR4(human)-SNAP-Neo |
| Insert Size (bp) | 2520bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCGC CACCATGATG TCTGCCTCGC GCCTGGCTGG GACTCTGATC CCAGCCATGG CCTTCCTCTC CTGCGTGAGA CCAGAAAGCT GGGAGCCCTG CGTGGAGGTG GTTCCTAATA TTACTTATCA ATGCATGGAG CTGAATTTCT ACAAAATCCC CGACAACCTC CCCTTCTCAA CCAAGAACCT GGACCTGAGC TTTAATCCCC TGAGGCATTT AGGCAGCTAT AGCTTCTTCA GTTTCCCAGA ACTGCAGGTG CTGGATTTAT CCAGGTGTGA AATCCAGACA ATTGAAGATG GGGCATATCA GAGCCTAAGC CACCTCTCTA CCTTAATATT GACAGGAAAC CCCATCCAGA GTTTAGCCCT GGGAGCCTTT TCTGGACTAT CAAGTTTACA GAAGCTGGTG GCTGTGGAGA CAAATCTAGC ATCTCTAGAG AACTTCCCCA TTGGACATCT CAAAACTTTG AAAGAACTTA ATGTGGCTCA CAATCTTATC CAATCTTTCA AATTACCTGA GTATTTTTCT AATCTGACCA ATCTAGAGCA CTTGGACCTT TCCAGCAACA AGATTCAAAG TATTTATTGC ACAGACTTGC GGGTTCTACA TCAAATGCCC CTACTCAATC TCTCTTTAGA CCTGTCCCTG AACCCTATGA ACTTTATCCA ACCAGGTGCA TTTAAAGAAA TTAGGCTTCA TAAGCTGACT TTAAGAAATA ATTTTGATAG TTTAAATGTA ATGAAAACTT GTATTCAAGG TCTGGCTGGT TTAGAAGTCC ATCGTTTGGT TCTGGGAGAA TTTAGAAATG AAGGAAACTT GGAAAAGTTT GACAAATCTG CTCTAGAGGG CCTGTGCAAT TTGACCATTG AAGAATTCCG ATTAGCATAC TTAGACTACT ACCTCGATGA TATTATTGAC TTATTTAATT GTTTGACAAA TGTTTCTTCA TTTTCCCTGG TGAGTGTGAC TATTGAAAGG GTAAAAGACT TTTCTTATAA TTTCGGATGG CAACATTTAG AATTAGTTAA CTGTAAATTT GGACAGTTTC CCACATTGAA ACTCAAATCT CTCAAAAGGC TTACTTTCAC TTCCAACAAA GGTGGGAATG CTTTTTCAGA AGTTGATCTA CCAAGCCTTG AGTTTCTAGA TCTCAGTAGA AATGGCTTGA GTTTCAAAGG TTGCTGTTCT CAAAGTGATT TTGGGACAAC CAGCCTAAAG TATTTAGATC TGAGCTTCAA TGGTGTTATT ACCATGAGTT CAAACTTCTT GGGCTTAGAA CAACTAGAAC ATCTGGATTT CCAGCATTCC AATTTGAAAC AAATGAGTGA GTTTTCAGTA TTCCTATCAC TCAGAAACCT CATTTACCTT GACATTTCTC ATACTCACAC CAGAGTTGCT TTCAATGGCA TCTTCAATGG CTTGTCCAGT CTCGAAGTCT TGAAAATGGC TGGCAATTCT TTCCAGGAAA ACTTCCTTCC AGATATCTTC ACAGAGCTGA GAAACTTGAC CTTCCTGGAC CTCTCTCAGT GTCAACTGGA GCAGTTGTCT CCAACAGCAT TTAACTCACT CTCCAGTCTT CAGGTACTAA ATATGAGCCA CAACAACTTC TTTTCATTGG ATACGTTTCC TTATAAGTGT CTGAACTCCC TCCAGGTTCT TGATTACAGT CTCAATCACA TAATGACTTC CAAAAAACAG GAACTACAGC ATTTTCCAAG TAGTCTAGCT TTCTTAAATC TTACTCAGAA TGACTTTGCT TGTACTTGTG AACACCAGAG TTTCCTGCAA TGGATCAAGG ACCAGAGGCA GCTCTTGGTG GAAGTTGAAC GAATGGAATG TGCAACACCT TCAGATAAGC AGGGCATGCC TGTGCTGAGT TTGAATATCA CCTGTCAGAT GAATAAGACC ATCATTGGTG TGTCGGTCCT CAGTGTGCTT GTAGTATCTG TTGTAGCAGT TCTGGTCTAT AAGTTCTATT TTCACCTGAT GCTTCTTGCT GGCTGCATAA AGTATGGTAG AGGTGAAAAC ATCTATGATG CCTTTGTTAT CTACTCAAGC CAGGATGAGG ACTGGGTAAG GAATGAGCTA GTAAAGAATT TAGAAGAAGG GGTGCCTCCA TTTCAGCTCT GCCTTCACTA CAGAGACTTT ATTCCCGGTG TGGCCATTGC TGCCAACATC ATCCATGAAG GTTTCCATAA AAGCCGAAAG GTGATTGTTG TGGTGTCCCA GCACTTCATC CAGAGCCGCT GGTGTATCTT TGAATATGAG ATTGCTCAGA CCTGGCAGTT TCTGAGCAGT CGTGCTGGTA TCATCTTCAT TGTCCTGCAG AAGGTGGAGA AGACCCTGCT CAGGCAGCAG GTGGAGCTGT ACCGCCTTCT CAGCAGGAAC ACTTACCTGG AGTGGGAGGA CAGTGTCCTG GGGCGGCACA TCTTCTGGAG ACGACTCAGA AAAGCCCTGC TGGATGGTAA ATCATGGAAT CCAGAAGGAA CAGTGGGTAC AGGATGCAAT TGGCAGGAAG CAACATCTAT CGGCGGTGGA GGTTCTGGTG GAGGCGGATC AGAATTCATG GACAAAGATT GCGAAATGAA ACGTACCACC CTGGATAGCC CGCTGGGCAA ACTGGAACTG AGCGGCTGCG AACAGGGCCT GCATGAAATT AAACTGCTGG GTAAAGGCAC CAGCGCGGCC GATGCGGTTG AAGTTCCGGC CCCGGCCGCC GTGCTGGGTG GTCCGGAACC GCTGATGCAG GCGACCGCGT GGCTGAACGC GTATTTTCAT CAGCCGGAAG CGATTGAAGA ATTTCCGGTT CCGGCGCTGC ATCATCCGGT GTTTCAGCAG GAGAGCTTTA CCCGTCAGGT GCTGTGGAAA CTGCTGAAAG TGGTTAAATT TGGCGAAGTG ATTAGCTATC AGCAGCTGGC GGCCCTGGCG GGTAATCCGG CGGCCACCGC CGCCGTTAAA ACCGCGCTGA GCGGTAACCC GGTGCCGATT CTGATTCCGT GCCATCGTGT GGTTAGCTCT AGCGGTGCGG TTGGCGGTTA TGAAGGTGGT CTGGCGGTGA AAGAGTGGCT GCTGGCCCAT GAAGGTCATC GTCTGGGTAA ACCGGGTCTG GGATGACTCG AGTCTAGAGG CCCCTTAAGG GTTCGATCCC TACCGGTTAG TAATGAGTTT GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG
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