pSV40-hRluc-PDCD4(human)-3UTR-mut-TK-Fluc
$84.00
In stock
SKU
PL45435
100μg liquid plasmid(Instantly):$105
| name | pSV40-hRluc-PDCD4(human)-3UTR-mut-TK-Fluc | another name | apSV40-hRluc-PDCD4(human)-3UTR-mut-TK-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | psiCHECK-2-F：GAAGTTCCCTAACACCGAGT | |
| 3′ sequencing primer | Psicheck-2-R：CCCCCGCGAGGTCCGAAGAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGGT AAGCGAAGGA GATGGAGGTC GTCTTAAACC AGAGAGCTAC TGAATATAAG AACTCTTGCA GTCTTAGATG TTATAAAAAT ATATATCTGA ATTGTAAGAG TTGTTAGCAC AAGTTTTTTT TTTTTTTTTT TTTAAGCACT TGTTTTGGGT ACAAGGCATT TCTGACATTT TATAAACCTA CATTTAAGGG GAATTTTTAA AGGAAATGTT TTTTCTTTTT TTTTTGTTTT TCGAGGGGGC AAGGAGGGAC AGAAAAGTAA CCTCTTCTTA AGTGGAATAT TCTATATTCG AACCTTTTGT AAGTGCCATG TTTATTATCT AATCATTCCA AGTTTTGCAT TGATGTCTGA CTGCCACTCC TTTCTTTCAA GGACAGTGTT TTTTGTAGTA AAATCACTGG TTTATACAAA GCTTTATTTA GGGGGTAAAG TTAAGCTGCT AAAACCCCAT GTTGGCTGCT GCTGTTGAGA TACTGTGCTT TGGGAGTAAA AAAAGAAAGT TATTTCTTTG TCTTAAAGAA TTTTTAAAAA ATTAGTCATG AGACTTATTC ATCTTTCCAG GGAACATACT GATTGGTGCG GCCGCTGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAGGATCTAA ATGAGTCTTC GGACCTCGCG GGGGCCGCTT AAGCGGTGGT TAGGGTTTGT CTGACGCGGG GGGAGGGGGA AGGAACGAAA CACTCTCATT CGGAGGCGGC TCGGGGTTTG GTCTTGGTGG CCACGGGCAC GCAGAAGAGC GCCGCGATCC TCTTAAGCAC CCCCCCGCCC TCCGTGGAGG CGGGGGTTTG GTCGGCGGGT GGTAACTGGC GGGCCGCTGA CTCGGGCGGG TCGCGCGCCC CAGAGTGTGA CCTTTTCGGT CTGCTCGCAG ACCCCCGGGC GGCGCCGCCG CGGCGGCGAC GGGCTCGCTG GGTCCTAGGC TCCATGGGGA CCGTATACGT GGACAGGCTC TGGAGCATCC GCACGACTGC GGTGATATTA CCGGAGACCT TCTGCGGGAC GAGCCGGGTC ACGCGGCTGA CGCGGAGCGT CCGTTGGGCG ACAAACACCA GGACGGGGCA CAGGTACACT ATCTTGTCAC CCGGAGGCGC GAGGGACTGC AGGAGCTTCA GGGAGTGGCG CAGCTGCTTC ATCCCCGTGG CCCGTTGCTC GCGTTTGCTG GCGGTGTCCC CGGAAGAAAT ATATTTGCAT GTCTTTAGTT CTATGATGAC ACAAACCCCG CCCAGCGTCT TGTCATTGGC GAATTCGAAC ACGCAGATGC AGTCGGGGCG GCGCGGTCCC AGGTCCACTT CGCATATTAA GGTGACGCGT GTGGCCTCGA ACACCGAGCG ACCCTGCAGC GACCCGCTTA AAAGCTTGGC ATTCCGGTAC TGTTGGTAAA GCCACCATGG CCGATGCTAA GAACATTAAG AAGGGCCCTG CTCCCTTCTA CCCTCTGGAG GATGGCACCG CTGGCGAGCA GCTGCACAAG GCCATGAAGA GGTATGCCCT GGTGCCTGGC ACCATTGCCT TCACCGATGC CCACATTGAG GTGGACATCA CCTATGCCGA GTACTTCGAG ATGTCTGTGC GCCTGGCCGA GGCCATGAAG AGGTACGGCC TGAACACCAA CCACCGCATC GTGGTGTGCT CTGAGAACTC TCTGCAGTTC TTCATGCCAG TGCTGGGCGC CCTGTTCATC GGAGTGGCCG TGGCCCCTGC TAACGACATT TACAACGAGC GCGAGCTGCT GAACAGCATG GGCATTTCTC AGCCTACCGT GGTGTTCGTG TCTAAGAAGG GCCTGCAGAA GATCCTGAAC GTGCAGAAGA AGCTGCCTAT CATCCAGAAG ATCATCATCA TGGACTCTAA GACCGACTAC CAGGGCTTCC AGAGCATGTA CACATTCGTG ACATCTCATC TGCCTCCTGG CTTCAACGAG TACGACTTCG TGCCAGAGTC TTTCGACAGG GACAAAACCA TTGCCCTGAT CATGAACAGC TCTGGGTCTA CCGGCCTGCC TAAGGGCGTG GCCCTGCCTC ATCGCACCGC CTGTGTGCGC TTCTCTCACG CCCGCGACCC TATTTTCGGC AACCAGATCA TCCCCGACAC CGCTATTCTG AGCGTGGTGC CATTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGATTTGC GGCTTTCGGG TGGTGCTGAT GTACCGCTTC GAGGAGGAGC TGTTCCTGCG CAGCCTGCAA GACTACAAAA TTCAGTCTGC CCTGCTGGTG CCAACCCTGT TCAGCTTCTT CGCTAAGAGC ACCCTGATCG ACAAGTACGA CCTGTCTAAC CTGCACGAGA TTGCCTCTGG CGGCGCCCCA CTGTCTAAGG AGGTGGGCGA AGCCGTGGCC AAGCGCTTTC ATCTGCCAGG CATCCGCCAG GGCTACGGCC TGACCGAGAC AACCAGCGCC ATTCTGATTA CCCCAGAGGG CGACGACAAG CCTGGCGCCG TGGGCAAGGT GGTGCCATTC TTCGAGGCCA AGGTGGTGGA CCTGGACACC GGCAAGACCC TGGGAGTGAA CCAGCGCGGC GAGCTGTGTG TGCGCGGCCC TATGATTATG TCCGGCTACG TGAATAACCC TGAGGCCACA AACGCCCTGA TCGACAAGGA CGGCTGGCTG CACTCTGGCG ACATTGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGCCTG AAGTCTCTGA TCAAGTACAA GGGCTACCAG GTGGCCCCAG CCGAGCTGGA GTCTATCCTG CTGCAGCACC CTAACATTTT CGACGCCGGA GTGGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCTG CCGCCGTCGT CGTGCTGGAA CACGGCAAGA CCATGACCGA GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTGACAACC GCCAAGAAGC TGCGCGGCGG AGTGGTGTTC GTGGACGAGG TGCCCAAGGG CCTGACCGGC AAGCTGGACG CCCGCAAGAT CCGCGAGATC CTGATCAAGG CTAAGAAAGG CGGCAAGATC GCCGTGTAAT AATTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC GATAAGGATC CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GGCTCGAC
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