pENTR223.1-ABCA12(human)
$157.00
In stock
SKU
PL45202
NM_173076.3;ARCI4A; ARCI4B; ICR2B; LI2
100μg liquid plasmid(Instantly):$178
| name | pENTR223.1-ABCA12(human) | another name | apENTR223.1-ABCA12(human) |
| Insert Size (bp) | 7786bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATACGCG TACCGCTAGC CAGGAAGAGT TTGTAGAAAC GCAAAAAGGC CATCCGTCAG GATGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTTATGGC GGGCGTCCTG CCCGCCACCC TCCGGGCCGT TGCTTCACAA CGTTCAAATC CGCTCCCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTCCGACT GAGCCTTTCG TTTTATTTGA TGCCTGGCAG TTCCCTACTC TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GAAGGGCCGT CAAGGCCCAC CATGGCTTCC CTGTTTCATC AGCTTCAGAT CCTGGTCTGG AAAAATTGGC TAGGTGTAAA AAGGCAGCCG CTTTGGACAC TTGTCTTGAT CTTATGGCCA GTCATTATTT TCATAATTTT GGCTATTACT CGGACCAAAT TTCCTCCAAC TGCAAAACCA ACTTGTTACC TCGCACCTCG AAACCTTCCT AGTACTGGAT TCTTTCCATT CCTGCAGACC CTACTCTGTG ACACAGACTC TAAATGCAAA GACACACCCT ATGGCCCACA AGATCTGCTT CGTAGGAAAG GAATTGATGA TGCACTATTT AAAGACAGTG AGATTCTGAG AAAGTCATCC AACCTGGATA AGGACAGCAG TTTATCATTC CAGAGCACCC AAGTTCCAGA AAGAAGGCAT GCATCACTAG CCACAGTATT TCCCAGTCCA AGTTCTGATT TGGAAATCCC CGGAACATAT ACTTTCAATG GCAGTCAAGT GCTCGCACGA ATTCTTGGCT TGGAAAAGCT GTTAAAGCAA AATTCAACTT CAGAAGATAT ACGAAGAGAA CTATGTGACA GCTATTCAGG ATACATTGTG GATGATGCCT TCTCTTGGAC CTTTCTAGGA AGAAATGTTT TTAACAAATT TTGCCTTTCT AACATGACCC TTTTAGAGTC TTCTCTCCAA GAACTAAACA AACAGTTCTC CCAGCTATCC AGTGACCCCA ACAATCAGAA GATAGTGTTT CAGGAAATAG TCAGAATGCT GTCTTTCTTC TCACAAGTGC AAGAGCAGAA AGCTGTGTGG CAGCTTCTGT CTAGTTTTCC AAATGTGTTT CAGAATGACA CATCACTAAG CAATCTATTT GATGTTCTTC GAAAGGCAAA CAGTGTGCTG CTGGTTGTGC AGAAGGTTTA TCCACGTTTT GCAACTAACG AAGGTTTCAG AACCCTCCAG AAGTCTGTTA AACATCTGCT GTACACTCTG GACTCCCCAG CTCAAGGTGA CTCCGATAAT ATAACGCATG TGTGGAATGA GGATGATGGA CAGACCTTAT CTCCAAGCAG TCTGGCTGCA CAGCTCCTAA TTCTGGAAAA CTTTGAAGAT GCCCTCTTAA ATATATCAGC AAATAGTCCT TATATTCCTT ACTTGGCATG TGTGAGAAAT GTGACTGACA GTTTGGCCAG AGGTTCACCA GAAAATCTAA GACTCCTGCA GTCCACAATA CGATTTAAAA AATCTTTTCT TCGCAATGGT TCCTATGAAG ATTACTTTCC TCCAGTTCCT GAAGTCCTAA AATCAAAACT GTCTCAACTT CGAAACTTGA CCGAACTTCT TTGTGAATCT GAAACTTTCA GTTTGATAGA GAAGTCATGC CAGCTCTCTG ATATGAGCTT TGGGAGCCTG TGTGAAGAAA GTGAGTTTGA TCTGCAACTC CTCGAAGCGG CAGAGCTGGG CACCGAAATA GCAGCCAGCT TACTGTACCA TGACAATGTC ATATCTAAAA AAGTGAGAGA TTTGCTGACT GGAGATCCAA GCAAAATTAA TTTAAATATG GATCAGTTTC TAGAACAGGC ACTGCAAATG AATTACTTGG AAAATATCAC TCAGTTAATA CCGATCATAG AAGCCATGCT GCATGTCAAT AACAGTGCAG ATGCTTCTGA AAAGCCAGGT CAGTTACTAG AAATGTTTAA AAATGTTGAA GAGCTGAAAG AAGATTTAAG GAGAACAACA GGAATGTCCA ACAGGACTAT TGACAAGTTG CTGGCCATTC CCATCCCTGA TAATAGAGCT GAGATTATTT CTCAGGTGTT CTGGCTGCAT TCCTGTGATA CTAATATCAC CACTCCCAAA CTAGAAGATG CAATGAAAGA ATTCTGCAAC CTGTCTCTTT CAGAGAGATC CCGGCAGTCT TACCTCATCG GACTCACCCT TCTGCACTAC TTAAACATTT ACAACTTCAC ATACAAGGTG TTTTTCCCGA GGAAAGATCA AAAGCCAGTA GAAAAGATGA TGGAGCTCTT CATAAGACTA AAAGAGATTC TCAATCAGAT GGCTTCTGGC ACACATCCGC TGCTAGACAA AATGAGATCC CTGAAGCAAA TGCATCTGCC CAGAAGTGTT CCATTAACAC AGGCAATGTA CAGAAGCAAC CGAATGAACA CACCACAAGG ATCATTTAGC ACCATCTCCC AAGCATTATG TTCTCAAGGA ATTACCACTG AATATTTAAC TGCCATGCTG CCCTCTTCCC AGAGGCCAAA AGGCAACCAC ACCAAGGATT TTTTGACTTA TAAATTAACT AAAGAGCAAA TTGCTTCAAA ATATGGAATT CCCATAAATT CCACACCATT TTGCTTCTCC CTTTATAAAG ACATCATTAA CATGCCCGCT GGACCTGTGA TTTGGGCTTT CTTGAAACCT ATGTTGTTGG GAAGAATTTT GTATGCACCA TATAACCCAG TCACAAAGGC AATAATGGAA AAGTCCAATG TAACTCTGAG ACAGCTGGCG GAATTAAGAG AAAAATCTCA AGAGTGGATG GATAAGTCGC CACTTTTCAT GAATTCCTTC CATCTGTTAA ACCAGGCAAT TCCAATGCTC CAGAATACTC TAAGGAACCC TTTTGTGCAA GTTTTTGTAA AGTTCTCCGT GGGACTCGAT GCTGTTGAAC TATTGAAACA GATAGATGAA CTCGATATTC TAAGACTGAA ATTAGAGAAC AACATTGACA TCATCGATCA GCTTAACACA CTATCTTCCC TGACAGTAAA TATTTCCTCT TGTGTATTAT ATGACCGTAT TCAGGCAGCA AAAACCATAG ATGAAATGGA GAGAGAGGCT AAAAGGCTCT ACAAAAGCAA CGAACTCTTT GGAAGTGTTA TTTTTAAGCT TCCTTCTAAC AGAAGCTGGC ACAGAGGCTA TGACTCTGGA AATGTCTTTC TTCCTCCTGT CATAAAATAT ACCATCCGGA TGAGTCTCAA GACCGCACAG ACCACAAGAA GCCTAAGAAC CAAGATTTGG GCTCCAGGGC CACACAATTC TCCATCACAC AACCAGATCT ATGGCAGGGC TTTTATTTAT TTACAGGATA GTATTGAAAG AGCAATCATT GAATTGCAAA CTGGAAGGAA CTCCCAGGAA ATAGCAGTCC AGGTTCAAGC AATTCCTTAT CCCTGCTTCA TGAAAGACAA CTTCCTAACC AGTGTCTCTT ATTCTCTTCC AATTGTGCTT ATGGTTGCCT GGGTTGTATT TATAGCTGCC TTTGTAAAAA AGCTTGTCTA TGAGAAAGAC CTCCGGCTTC ATGAGTACAT GAAGATGATG GGTGTGAACT CCTGCAGCCA TTTCTTTGCC TGGCTTATAG AGAGTGTTGG ATTTTTACTG GTTACCATCG TGATCCTCAT CATTATACTC AAGTTTGGCA ATATTCTTCC TAAAACAAAT GGGTTCATTT TGTTCCTGTA TTTTTCGGAC TACAGCTTCT CGGTTATTGC CATGAGCTAT CTTATCAGTG TCTTCTTCAA CAACACCAAC ATTGCAGCTC TGATCGGAAG CCTCATCTAC ATCATTGCCT TCTTTCCATT TATTGTTCTG GTTACAGTGG AGAATGAGTT GAGCTATGTA TTGAAAGTGT TCATGAGCCT GCTGTCCCCA ACAGCATTCA GCTATGCAAG CCAATACATT GCACGATACG AAGAACAGGG CATTGGTCTT CAGTGGGAAA ATATGTACAC CTCCCCGGTT CAGGATGACA CCACCTCATT TGGCTGGCTG TGCTGTCTAA TCCTAGCTGA CTCTTTCATT TATTTCCTTA TTGCTTGGTA TGTCAGGAAT GTCTTCCCAG GGACATACGG TATGGCAGCT CCCTGGTATT TTCCAATTCT TCCTTCCTAT TGGAAGGAGC GATTTGGGTG TGCAGAGGTG AAGCCTGAGA AGAGCAATGG CCTCATGTTT ACTAACATCA TGATGCAGAA CACCAACCCA TCTGCCAGTC CTGAATACAT GTTTTCCTCT AACATCGAGC CTGAACCTAA AGATCTCACA GTCGGGGTTG CCCTGCATGG GGTCACAAAG ATCTATGGCT CAAAAGTTGC TGTTGATAAC CTCAATCTGA ACTTTTATGA AGGGCATATT ACTTCATTGC TGGGGCCCAA TGGAGCTGGG AAAACTACTA CCATTTCCAT GTTAACTGGG CTGTTTGGGG CCTCAGCAGG CACCATTTTT GTATATGGAA AAGATATCAA AACAGACCTA CACACGGTAC GGAAGAACAT GGGAGTCTGT ATGCAGCACG ACGTCTTGTT CAGTTACCTC ACTACTAAGG AGCACCTTCT CCTATATGGT TCCATCAAAG TTCCTCACTG GACTAAAAAG CAGCTCCACG AGGAAGTAAA AAGGACTTTA AAAGATACTG GACTATATAG CCATCGTCAT AAGAGAGTTG GAACACTGTC AGGAGGCATG AAGAGGAAGT TATCTATATC CATAGCTCTC ATTGGTGGAT CAAGGGTAGT AATTTTGGAT GAACCATCTA CTGGAGTTGA CCCATGTTCT CGCCGAAGTA TATGGGATGT TATATCCAAG AACAAAACTG CCAGAACAAT CATTCTGTCA ACGCACCACT TGGACGAGGC TGAAGTGCTG AGTGACCGCA TCGCCTTCCT GGAGCAGGGT GGGCTTAGGT GCTGTGGGTC CCCATTTTAC CTCAAGGAAG CCTTTGGCGA TGGGTATCAC CTCACGCTTA CCAAGAAGAA GAGTCCAAAT TTAAATGCAA ATGCAGTATG TGACACCATG GCCGTGACAG CAATGATCCA ATCACATCTC CCCGAAGCCT ACCTCAAGGA GGATATTGGG GGAGAGCTTG TTTATGTACT TCCTCCATTC AGCACCAAAG TCTCAGGGGC CTACCTGTCA CTCCTACGGG CACTCGACAA TGGCATGGGT GACCTCAACA TCGGGTGCTA CGGCATTTCA GATACCACCG TGGAGGAGGT CTTTCTGAAC TTGACCAAAG AGTCACAAAA AAATAGTGCT ATGAGTCTTG AGCACTTAAC ACAAAAGAAA ATTGGGAATT CCAATGCCAA TGGCATCTCA ACTCCTGACG ATTTATCTGT GAGCAGCAGC AATTTCACAG ACAGAGATGA CAAAATCCTG ACAAGAGGAG AGAGGCTGGA TGGCTTTGGA CTGTTGCTGA AGAAGATCAT GGCTATACTC ATCAAGAGGT TCCACCACAC CCGCAGGAAC TGGAAAGGTC TCATTGCTCA GGTTATCCTC CCCATCGTCT TTGTTACCAC TGCCATGGGC CTTGGCACAC TGAGAAATTC CAGCAACAGT TATCCAGAGA TTCAGATCTC CCCCTCTCTT TATGGTACCT CCGAACAGAC AGCCTTCTAT GCTAATTATC ACCCGAGCAC GGAAGCACTT GTCTCAGCAA TGTGGGACTT CCCTGGAATT GACAACATGT GTCTGAACAC CAGTGATCTA CAGTGTTTAA ACAAAGACAG TCTGGAAAAA TGGAACACCA GTGGAGAACC CATCACTAAT TTTGGTGTTT GCTCCTGCTC AGAAAATGTC CAGGAATGTC CTAAATTTAA CTATTCCCCA CCGCACAGAA GAACTTACTC ATCCCAGGTA ATTTATAACC TCACTGGGCA ACGAGTGGAA AATTATCTTA TATCAACTGC AAATGAGTTT GTCCAAAAAA GATATGGAGG TTGGAGTTTT GGGCTGCCTT TGACAAAAGA CCTTCGTTTT GATATAACAG GAGTCCCTGC CAATAGAACA CTTGCCAAGG TATGGTATGA TCCAGAAGGC TATCACTCCC TTCCAGCTTA CCTCAACAGC CTGAATAATT TCCTTCTGCG AGTTAACATG TCAAAATACG ATGCTGCCCG ACATGGCATC ATCATGTATA GCCATCCTTA TCCAGGAGTG CAAGACCAAG AACAAGCCAC AATCAGCAGT TTAATCGATA TTTTAGTGGC ACTGTCTATC TTGATGGGCT ACTCTGTCAC CACCGCCAGC TTTGTCACCT ATGTTGTAAG GGAACATCAA ACCAAAGCCA AACAGTTGCA GCACATTTCA GGCATTGGCG TGACATGCTA CTGGGTAACA AACTTCATTT ATGACATGGT TTTCTACTTG GTGCCTGTAG CGTTTTCAAT TGGTATCATT GCGATTTTCA AATTACCTGC ATTCTACAGT GAAAACAACC TAGGCGCTGT ATCTCTCCTA CTTCTCCTGT TTGGGTATGC AACATTTTCC TGGATGTACT TGCTGGCTGG GCTCTTCCAT GAAACAGGAA TGGCCTTCAT CACTTACGTC TGTGTCAACT TGTTTTTTGG CATTAATTCC ATTGTTTCCC TGTCAGTGGT ATACTTTCTT TCCAAGGAAA AGCCTAATGA TCCGACTTTA GAACTTATTT CTGAAACCCT CAAGCGCATT TTCCTGATTT TCCCACAATT CTGTTTTGGC TACGGTTTGA TTGAACTTTC TCAACAACAG TCGGTCCTAG ACTTCTTAAA AGCATATGGA GTGGAATACC CAAATGAAAC CTTTGAGATG AATAAACTAG GTGCAATGTT TGTGGCTTTG GTTTCTCAGG GCACCATGTT TTTTTCCTTG CGACTCTTAA TCAACGAATC CCTGATAAAG AAACTCAGGC TTTTCTTCAG AAAATTTAAT TCTTCACATG TAAGGGAGAC AATAGATGAG GATGAAGATG TGCGGGCTGA GAGATTAAGA GTTGAGAGTG GTGCAGCTGA ATTTGACTTG GTCCAACTTT ATTGTCTCAC AAAGACCTAC CAACTTATCC ACAAAAAGAT TATAGCTGTA AACAACATCA GCATTGGGAT ACCTGCTGGA GAGTGTTTTG GGCTTCTTGG AGTGAATGGA GCAGGAAAGA CCACTATATT CAAGATGCTG ACAGGAGACA TCATTCCTTC AAGTGGAAAC ATTCTGATCA GAAATAAGAC CGGATCTCTG GGTCACGTTG ATTCTCACAG CTCATTAGTT GGCTACTGTC CTCAGGAAGA TGCCTTAGAT GACCTGGTAA CTGTGGAAGA ACATTTGTAT TTCTATGCCA GGGTACATGG AATTCCAGAA AAGGATATTA AAGAAACTGT TCATAAACTC CTTAGGAGAC TTCACCTGAT GCCCTTCAAG GACAGAGCTA CCTCTATGTG CAGTTATGGC ACAAAAAGAA AATTATCCAC TGCACTGGCC TTGATAGGGA AACCTTCCAT TCTACTGCTG GATGAGCCGA GCTCTGGCAT GGATCCGAAG TCGAAACGGC ACCTCTGGAA GATCATTTCA GAAGAAGTAC AGAACAAATG TTCCGTCATC CTCACATCTC ACAGCATGGA AGAATGTGAA GCTCTCTGTA CCAGGTTGGC CATTATGGTG AATGGAAAGT TTCAATGTAT TGGATCTTTG CAGCACATAA AGAGCAGGTT TGGACGAGGA TTTACTGTCA AAGTTCACTT GAAGAATAAC AAAGTGACCA TGGAGACCCT CACAAAGTTC ATGCAGCTGC ACTTTCCAAA AACATACTTA AAAGATCAGC ACCTCAGCAT GCTAGAGTAT CATGTACCAG TCACAGCAGG AGGAGTCGCA AACATTTTTG ATCTGCTGGA AACCAACAAG ACTGCTTTAA ATATTACAAA TTTCTTAGTG AGTCAGACCA CTCTGGAAGA GGTTTTCATC AACTTTGCCA AAGACCAGAA GTCCTATGAA ACTGCTGATA CCAGCAGCCA AGGTTCCACT ATAAGTGTTG ACTCACAAGA TGACCAGATG GAGTCTTCAG GCCTCATGGG CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAAAAATAAT TGCTCATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT CGCTGCTGCC CAAGGTTGCC GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC AGTGGACATA AGCCTGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG CTTGTTATGA CTGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAACC CTCGAGCCAC CCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAA
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