NM_005562.3;B2T; BM600; CSF; EBR2; EBR2A; JEB3A; JEB3B;LAMB2T; LAMNB2
100μg liquid plasmid(Instantly):$120
| name | pCMV-3×FLAG-LAMC2(human) | another name | apCMV-3×FLAG-LAMC2(human) |
| Insert Size (bp) | 3582bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTAACCATG GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGATTACAA GGATGACGAT GACAAGCTTG CGGCCGCGAA TTCCATGCCT GCGCTCTGGC TGGGCTGCTG CCTCTGCTTC TCGCTCCTCC TGCCCGCAGC CCGGGCCACC TCCAGGAGGG AAGTCTGTGA TTGCAATGGG AAGTCCAGGC AGTGTATCTT TGATCGGGAA CTTCACAGAC AAACTGGTAA TGGATTCCGC TGCCTCAACT GCAATGACAA CACTGATGGC ATTCACTGCG AGAAGTGCAA GAATGGCTTT TACCGGCACA GAGAAAGGGA CCGCTGTTTG CCCTGCAATT GTAACTCCAA AGGTTCTCTT AGTGCTCGAT GTGACAACTC CGGACGGTGC AGCTGTAAAC CAGGTGTGAC AGGAGCCAGA TGCGACCGAT GTCTGCCAGG CTTCCACATG CTCACGGATG CGGGGTGCAC CCAAGACCAG AGACTGCTAG ACTCCAAGTG TGACTGTGAC CCAGCTGGCA TCGCAGGGCC CTGTGACGCG GGCCGCTGTG TCTGCAAGCC AGCTGTCACT GGAGAACGCT GTGATAGGTG TCGATCAGGT TACTATAATC TGGATGGGGG GAACCCTGAG GGCTGTACCC AGTGTTTCTG CTATGGGCAT TCAGCCAGCT GCCGCAGCTC TGCAGAATAC AGTGTCCATA AGATCACCTC TACCTTTCAT CAAGATGTTG ATGGCTGGAA GGCTGTCCAA CGAAATGGGT CTCCTGCAAA GCTCCAATGG TCACAGCGCC ATCAAGATGT GTTTAGCTCA GCCCAACGAC TAGACCCTGT CTATTTTGTG GCTCCTGCCA AATTTCTTGG GAATCAACAG GTGAGCTATG GTCAAAGCCT GTCCTTTGAC TACCGTGTGG ACAGAGGAGG CAGACACCCA TCTGCCCATG ATGTGATTCT GGAAGGTGCT GGTCTACGGA TCACAGCTCC CTTGATGCCA CTTGGCAAGA CACTGCCTTG TGGGCTCACC AAGACTTACA CATTCAGGTT AAATGAGCAT CCAAGCAATA ATTGGAGCCC CCAGCTGAGT TACTTTGAGT ATCGAAGGTT ACTGCGGAAT CTCACAGCCC TCCGCATCCG AGCTACATAT GGAGAATACA GTACTGGGTA CATTGACAAT GTGACCCTGA TTTCAGCCCG CCCTGTCTCT GGAGCCCCAG CACCCTGGGT TGAACAGTGT ATATGTCCTG TTGGGTACAA GGGGCAATTC TGCCAGGATT GTGCTTCTGG CTACAAGAGA GATTCAGCGA GACTGGGGCC TTTTGGCACC TGTATTCCTT GTAACTGTCA AGGGGGAGGG GCCTGTGATC CAGACACAGG AGATTGTTAT TCAGGGGATG AGAATCCTGA CATTGAGTGT GCTGACTGCC CAATTGGTTT CTACAACGAT CCGCACGACC CCCGCAGCTG CAAGCCATGT CCCTGTCATA ACGGGTTCAG CTGCTCAGTG ATGCCGGAGA CGGAGGAGGT GGTGTGCAAT AACTGCCCTC CCGGGGTCAC CGGTGCCCGC TGTGAGCTCT GTGCTGATGG CTACTTTGGG GACCCCTTTG GTGAACATGG CCCAGTGAGG CCTTGTCAGC CCTGTCAATG CAACAACAAT GTGGACCCCA GTGCCTCTGG GAATTGTGAC CGGCTGACAG GCAGGTGTTT GAAGTGTATC CACAACACAG CCGGCATCTA CTGCGACCAG TGCAAAGCAG GCTACTTCGG GGACCCATTG GCTCCCAACC CAGCAGACAA GTGTCGAGCT TGCAACTGTA ACCCCATGGG CTCAGAGCCT GTAGGATGTC GAAGTGATGG CACCTGTGTT TGCAAGCCAG GATTTGGTGG CCCCAACTGT GAGCATGGAG CATTCAGCTG TCCAGCTTGC TATAATCAAG TGAAGATTCA GATGGATCAG TTTATGCAGC AGCTTCAGAG AATGGAGGCC CTGATTTCAA AGGCTCAGGG TGGTGATGGA GTAGTACCTG ATACAGAGCT GGAAGGCAGG ATGCAGCAGG CTGAGCAGGC CCTTCAGGAC ATTCTGAGAG ATGCCCAGAT TTCAGAAGGT GCTAGCAGAT CCCTTGGTCT CCAGTTGGCC AAGGTGAGGA GCCAAGAGAA CAGCTACCAG AGCCGCCTGG ATGACCTCAA GATGACTGTG GAAAGAGTTC GGGCTCTGGG AAGTCAGTAC CAGAACCGAG TTCGGGATAC TCACAGGCTC ATCACTCAGA TGCAGCTGAG CCTGGCAGAA AGTGAAGCTT CCTTGGGAAA CACTAACATT CCTGCCTCAG ACCACTACGT GGGGCCAAAT GGCTTTAAAA GTCTGGCTCA GGAGGCCACA AGATTAGCAG AAAGCCACGT TGAGTCAGCC AGTAACATGG AGCAACTGAC AAGGGAAACT GAGGACTATT CCAAACAAGC CCTCTCACTG GTGCGCAAGG CCCTGCATGA AGGAGTCGGA AGCGGAAGCG GTAGCCCGGA CGGTGCTGTG GTGCAAGGGC TTGTGGAAAA ATTGGAGAAA ACCAAGTCCC TGGCCCAGCA GTTGACAAGG GAGGCCACTC AAGCGGAAAT TGAAGCAGAT AGGTCTTATC AGCACAGTCT CCGCCTCCTG GATTCAGTGT CTCGGCTTCA GGGAGTCAGT GATCAGTCCT TTCAGGTGGA AGAAGCAAAG AGGATCAAAC AAAAAGCGGA TTCACTCTCA AGCCTGGTAA CCAGGCATAT GGATGAGTTC AAGCGTACAC AGAAGAATCT GGGAAACTGG AAAGAAGAAG CACAGCAGCT CTTACAGAAT GGAAAAAGTG GGAGAGAGAA ATCAGATCAG CTGCTTTCCC GTGCCAATCT TGCTAAAAGC AGAGCACAAG AAGCACTGAG TATGGGCAAT GCCACTTTTT ATGAAGTTGA GAGCATCCTT AAAAACCTCA GAGAGTTTGA CCTGCAGGTG GACAACAGAA AAGCAGAAGC TGAAGAAGCC ATGAAGAGAC TCTCCTACAT CAGCCAGAAG GTTTCAGATG CCAGTGACAA GACCCAGCAA GCAGAAAGAG CCCTGGGGAG CGCTGCTGCT GATGCACAGA GGGCAAAGAA TGGGGCCGGG GAGGCCCTGG AAATCTCCAG TGAGATTGAA CAGGAGATTG GGAGTCTGAA CTTGGAAGCC AATGTGACAG CAGATGGAGC CTTGGCCATG GAAAAGGGAC TGGCCTCTCT GAAGAGTGAG ATGAGGGAAG TGGAAGGAGA GCTGGAAAGG AAGGAGCTGG AGTTTGACAC GAATATGGAT GCAGTACAGA TGGTGATTAC AGAAGCCCAG AAGGTTGATA CCAGAGCCAA GAACGCTGGG GTTACAATCC AAGACACACT CAACACATTA GACGGCCTCC TGCATCTGAT GGACCAGCCT CTCAGTGTAG ATGAAGAGGG GCTGGTCTTA CTGGAGCAGA AGCTTTCCCG AGCCAAGACC CAGATCAACA GCCAACTGCG GCCCATGATG TCAGAGCTGG AAGAGAGGGC ACGTCAGCAG AGGGGCCACC TCCATTTGCT GGAGACAAGC ATAGATGGGA TTCTGGCTGA TGTGAAGAAC TTGGAGAACA TTAGGGACAA CCTGCCCCCA GGCTGCTACA ATACCCAGGC TCTTGAGCAA CAGTGAGGTA CCAGTCGACT CTAGAGGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGAGGAACT GAAAAACCAG AAAGTTAATT CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TT
