NM_201414.3;AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI;CTFgamma; CVAP; PN-II; PN2; preA4
100μg liquid plasmid(Instantly):$120
| name | pAAV-hSyn-APP(human)-EGFP | another name | apAAV-hSyn-APP(human)-EGFP |
| Insert Size (bp) | 2088bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | hSyn | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGTCCTGC AGGCAGCTGC GCGCTCGCTC GCTCACTGAG GCCGCCCGGG CGTCGGGCGA CCTTTGGTCG CCCGGCCTCA GTGAGCGAGC GAGCGCGCAG AGAGGGAGTG GCCAACTCCA TCACTAGGGG TTCCTGCGGC CGCACGCGTG TGTCTAGACT GCAGAGGGCC CTGCGTATGA GTGCAAGTGG GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT CGTGCCTGAG AGCGCAGTCG AGAAGGTACC GGATCCGCCA CCATGCTGCC CGGTTTGGCA CTGCTCCTGC TGGCCGCCTG GACGGCTCGG GCGCTGGAGG TACCCACTGA TGGTAATGCT GGCCTGCTGG CTGAACCCCA GATTGCCATG TTCTGTGGCA GACTGAACAT GCACATGAAT GTCCAGAATG GGAAGTGGGA TTCAGATCCA TCAGGGACCA AAACCTGCAT TGATACCAAG GAAGGCATCC TGCAGTATTG CCAAGAAGTC TACCCTGAAC TGCAGATCAC CAATGTGGTA GAAGCCAACC AACCAGTGAC CATCCAGAAC TGGTGCAAGC GGGGCCGCAA GCAGTGCAAG ACCCATCCCC ACTTTGTGAT TCCCTACCGC TGCTTAGTTG GTGAGTTTGT AAGTGATGCC CTTCTCGTTC CTGACAAGTG CAAATTCTTA CACCAGGAGA GGATGGATGT TTGCGAAACT CATCTTCACT GGCACACCGT CGCCAAAGAG ACATGCAGTG AGAAGAGTAC CAACTTGCAT GACTACGGCA TGTTGCTGCC CTGCGGAATT GACAAGTTCC GAGGGGTAGA GTTTGTGTGT TGCCCACTGG CTGAAGAAAG TGACAATGTG GATTCTGCTG ATGCGGAGGA GGATGACTCG GATGTCTGGT GGGGCGGAGC AGACACAGAC TATGCAGATG GGAGTGAAGA CAAAGTAGTA GAAGTAGCAG AGGAGGAAGA AGTGGCTGAG GTGGAAGAAG AAGAAGCCGA TGATGACGAG GACGATGAGG ATGGTGATGA GGTAGAGGAA GAGGCTGAGG AACCCTACGA AGAAGCCACA GAGAGAACCA CCAGCATTGC CACCACCACC ACCACCACCA CAGAGTCTGT GGAAGAGGTG GTTCGAGTTC CTACAACAGC AGCCAGTACC CCTGATGCCG TTGACAAGTA TCTCGAGACA CCTGGGGATG AGAATGAACA TGCCCATTTC CAGAAAGCCA AAGAGAGGCT TGAGGCCAAG CACCGAGAGA GAATGTCCCA GGTCATGAGA GAATGGGAAG AGGCAGAACG TCAAGCAAAG AACTTGCCTA AAGCTGATAA GAAGGCAGTT ATCCAGCATT TCCAGGAGAA AGTGGAATCT TTGGAACAGG AAGCAGCCAA CGAGAGACAG CAGCTGGTGG AGACACACAT GGCCAGAGTG GAAGCCATGC TCAATGACCG CCGCCGCCTG GCCCTGGAGA ACTACATCAC CGCTCTGCAG GCTGTTCCTC CTCGGCCTCG TCACGTGTTC AATATGCTAA AGAAGTATGT CCGCGCAGAA CAGAAGGACA GACAGCACAC CCTAAAGCAT TTCGAGCATG TGCGCATGGT GGATCCCAAG AAAGCCGCTC AGATCCGGTC CCAGGTTATG ACACACCTCC GTGTGATTTA TGAGCGCATG AATCAGTCTC TCTCCCTGCT CTACAACGTG CCTGCAGTGG CCGAGGAGAT TCAGGATGAA GTTGATGAGC TGCTTCAGAA AGAGCAAAAC TATTCAGATG ACGTCTTGGC CAACATGATT AGTGAACCAA GGATCAGTTA CGGAAACGAT GCTCTCATGC CATCTTTGAC CGAAACGAAA ACCACCGTGG AGCTCCTTCC CGTGAATGGA GAGTTCAGCC TGGACGATCT CCAGCCGTGG CATTCTTTTG GGGCTGACTC TGTGCCAGCC AACACAGAAA ACGAAGTTGA GCCTGTTGAT GCCCGCCCTG CTGCCGACCG AGGACTGACC ACTCGACCAG GTTCTGGGTT GACAAATATC AAGACGGAGG AGATCTCTGA AGTGAAGATG GATGCAGAAT TCCGACATGA CTCAGGATAT GAAGTTCATC ATCAAAAATT GGTGTTCTTT GCAGAAGATG TGGGTTCAAA CAAAGGTGCA ATCATTGGAC TCATGGTGGG CGGTGTTGTC ATAGCGACAG TGATCGTCAT CACCTTGGTG ATGCTGAAGA AGAAACAGTA CACATCCATT CATCATGGTG TGGTGGAGGT TGACGCCGCT GTCACCCCAG AGGAGCGCCA CCTGTCCAAG ATGCAGCAGA ACGGCTACGA AAATCCAACC TACAAGTTCT TTGAGCAGAT GCAGAACGGT GGAGGCGGTT CAGGCGGTGG AGGTAGTGGT GGAGGAGGCT CTATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AGGAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT ATGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGAGCGC TGCTCGAGAG ATCTACGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TGTAGGTAAC CACGTGCGGA CCGAGCGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCTGCCTGC AGGGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCTTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACT CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGTC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCA
