pCMV-SV40NLS-TadA8e-dAsCas12f1-3×FLAG
$84.00
In stock
SKU
PL45000
100μg liquid plasmid(Instantly):$105
| name | pCMV-SV40NLS-TadA8e-dAsCas12f1-3×FLAG | another name | apCMV-SV40NLS-TadA8e-dAsCas12f1-3×FLAG |
| Insert Size (bp) | 2709bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGCCAGATA TACGCGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC CGGGCCACCA TGGGAAAACG GACAGCCGAC GGAAGCGAGT TCGAGTCACC AAAGAAGAAG CGGAAAGTCT CTGAAGTCGA GTTTAGCCAC GAGTATTGGA TGAGGCACGC ACTGACCCTG GCAAAGCGAG CATGGGATGA AAGAGAAGTC CCCGTGGGCG CCGTGCTGGT GCACAACAAT AGAGTGATCG GAGAGGGATG GAACAGGCCA ATCGGCCGCC ACGACCCTAC CGCACACGCA GAGATCATGG CACTGAGGCA GGGAGGCCTG GTCATGCAGA ATTACCGCCT GATCGATGCC ACCCTGTATG TGACACTGGA GCCATGCGTG ATGTGCGCAG GAGCAATGAT CCACAGCAGG ATCGGAAGAG TGGTGTTCGG AGCACGGGAC GCCAAGACCG GCGCAGCAGG CTCCCTGATG GATGTGCTGC ACCACCCCGG CATGAACCAC CGGGTGGAGA TCACAGAGGG AATCCTGGCA GACGAGTGCG CCGCCCTGCT GAGCGATTTC TTTAGAATGC GGAGACAGGA GATCAAGGCC CAGAAGAAGG CACAGAGCTC CACCGACTCT GGAGGATCTA GCGGAGGATC CTCTGGAAGC GAGACACCAG GCACAAGCGA GTCCGCCACA CCAGAGAGCT CCGGCGGCTC CTCCGGAGGA TCCTCTGAGG TGGAGTTTTC CCACGAGTAC TGGATGAGAC ATGCCCTGAC CCTGGCCAAG AGGGCACGGG ATGAGAGGGA GGTGCCTGTG GGAGCCGTGC TGGTGCTGAA CAATAGAGTG ATCGGCGAGG GCTGGAACAG AGCCATCGGC CTGCACGACC CAACAGCCCA TGCCGAAATT ATGGCCCTGA GACAGGGCGG CCTGGTCATG CAGAACTACA GACTGATTGA CGCCACCCTG TACGTGACAT TCGAGCCTTG CGTGATGTGC GCCGGCGCCA TGATCCACTC TAGGATCGGC CGCGTGGTGT TTGGCGTGAG GAACTCAAAA AGAGGCGCCG CAGGCTCCCT GATGAACGTG CTGAACTACC CCGGCATGAA TCACCGCGTC GAAATTACCG AGGGAATCCT GGCAGATGAA TGTGCCGCCC TGCTGTGCGA TTTCTATCGG ATGCCTAGAC AGGTGTTCAA TGCTCAGAAG AAGGCCCAGA GCTCCATCAA CTCTGGAGGA TCTAGCGGAG GATCCTCTGG CAGCGAGACA CCAGGAACAA GCGAGTCAGC AACACCAGAG AGCAGTGGCG GCAGCAGCGG CGGCAGCCCC AAGAAAAAAC GCAAGGTGGG AAGCGGATCC ATGATCAAGG TGTACAGATA CGAGATCGTG AAGCCTCTGG ACCTGGACTG GAAGGAGTTC GGCACCATCC TGAGACAGCT GCAGCAGGAA ACCAGATTCG CCCTGAATAA GGCCACACAG CTGGCCTGGG AGTGGATGGG CTTCAGCAGC GACTACAAGG ATAACCACGG CGAGTACCCC AAGAGCAAGG ACATCCTGGG CTACACCAAC GTGCACGGCT ACGCCTACCA CACCATCAAG ACAAAGGCCT ACAGACTGAA CTCTGGAAAT CTGAGCCAGA CCATCAAGAG AGCCACAGAC AGGTTCAAGG CCTACCAGAA GGAGATCCTG CGCGGCGACA TGTCTATCCC CAGCTACAAG AGGGACATCC CCCTGGACCT GATCAAGGAG AACATCTCCG TGAACAGGAT GAATCACGGC GACTACATCG CCAGCCTGTC TCTGCTGAGC AACCCCGCCA AGCAGGAGAT GAACGTGAAG AGAAAGATCT CCGTGATCAT CATCGTGAGG GGCGCCGGCA AGACCATCAT GGACAGAATC CTGTCCGGCG AGTACCAGGT GAGCGCCAGC CAGATTATCC ACGACGACCG GAAGAACAAG TGGTACCTGA ACATCAGCTA CGACTTCGAG CCACAGACCA GAGTGCTGGA CCTGAACAAG ATCATGGGCA TTGCCCTGGG CGTGGCCGTG GCCGTGTACA TGGCCTTCCA GCACACCCCC GCCAGGTACA AGCTGGAGGG CGGCGAGATT GAGAACTTCA GGAGGCAGGT GGAGAGCCGG CGCATCTCCA TGCTGAGACA GGGCAAGTAC GCCGGCGGCG CCAGGGGCGG CCACGGCAGA GACAAGAGAA TCAAGCCCAT TGAGCAGCTG AGGGATAAGA TCGCCAATTT CAGAGACACC ACCAATCACC GGTACAGCAG ATACATCGTG GACATGGCCA TCAAGGAGGG CTGCGGCACA ATCCAGATGG AGGATCTGAC AAACATCAGA GACATCGGCA GCAGATTCCT GCAGAACTGG ACCTACTACG ACCTGCAGCA GAAGATCATC TACAAGGCCG AGGAGGCCGG CATCAAAGTG ATCAAGATCG ACCCCCAGTA CACCAGCCAG AGATGCTCCG AGTGCGGCAA CATCGACTCC GGCAACAGAA TCGGCCAGGC CATCTTTAAG TGCCGGGCCT GCGGCTACGA GGCCAACGCC GACTACAACG CCGCCCGGAA TATCGCCATC CCCAACATCG ACAAGATCAT CGCCGAGAGC ATTAAGTCTG GTGGTTCTGG TAGTGGGAGC AACGGCAGCA GCCTCCATAT GGGTGGAGGC TCCGGGGAAG ATCCTGCTGC CAAACGCGTT AAACTAGACG ACTATAAGGA CCACGACGGA GACTACAAGG ATCATGATAT TGATTACAAA GACGATGACG ATAAGTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCG ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TAGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCGATCTCCC GATCCCCTAG GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTAC
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