pCP20-Tet
$128.00
In stock
SKU
PL44988
100μg liquid plasmid(Instantly):$171
| name | pCP20-Tet | another name | apCP20-Tet |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Amp,Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pSC101 | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | pCP-F：TCCCAGCCCTGTGTATAAC | |
| 3′ sequencing primer | pCP-R：GGAAAGTTATCGCTAGTCAGTG | Copy number | Low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCTT GCTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG GAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA GATTCTTGCT CAATTGTTAT CAGCTATGCG CCGACCAGAA CACCTTGCCG ATCAGCCAAA CGTCTCTTCA GGCCACTGAC TAGCGATAAC TTTCCCCACA ACGGAACAAC TCTCATTGCA TGGGATCATT GGGTACTGTG GGTTTAGTGG TTGTAAAAAC ACCTGACCGC TATCCCTGAT CAGTTTCTTG AAGGTAAACT CATCACCCCC AAGTCTGGCT ATGCAGAAAT CACCTGGCTC AACAGCCTGC TCAGGGTCAA CGAGAATTAA CATTCCGTCA GGAAAGCTTG GCTTGGAGCC TGTTGGTGCG GTCATGGAAT TACCTTCAAC CTCAAGCCAG AATGCAGAAT CACTGGCTTT TTTGGTTGTG CTTACCCATC TCTCCGCATC ACCTTTGGTA AAGGTTCTAA GCTTAGGTGA GAACATCCCT GCCTGAACAT GAGAAAAAAC AGGGTACTCA TACTCACTTC TAAGTGACGG CTGCATACTA ACCGCTTCAT ACATCTCGTA GATTTCTCTG GCGATTGAAG GGCTAAATTC TTCAACGCTA ACTTTGAGAA TTTTTGTAAG CAATGCGGCG TTATAAGCAT TTAATGCATT GATGCCATTA AATAAAGCAC CAACGCCTGA CTGCCCCATC CCCATCTTGT CTGCGACAGA TTCCTGGGAT AAGCCAAGTT CATTTTTCTT TTTTTCATAA ATTGCTTTAA GGCGACGTGC GTCCTCAAGC TGCTCTTGTG TTAATGGTTT CTTTTTTGTG CTCATACGTT AAATCTATCA CCGCAAGGGA TAAATATCTA ACACCGTGCG TGTTGACTAT TTTACCTCTG GCGGTGATAA TGGTTGCATG TACTAAGGAG GTTGTATGCC ACAATTTGAT ATATTATGTA AAACACCACC TAAGGTGCTT GTTCGTCAGT TTGTGGAAAG GTTTGAAAGA CCTTCAGGTG AGAAAATAGC ATTATGTGCT GCTGAACTAA CCTATTTATG TTGGATGATT ACACATAACG GAACAGCAAT CAAGAGAGCC ACATTCATGA GCTATAATAC TATCATAAGC AATTCGCTGA GTTTCGATAT TGTCAATAAA TCACTCCAGT TTAAATACAA GACGCAAAAA GCAACAATTC TGGAAGCCTC ATTAAAGAAA TTGATTCCTG CTTGGGAATT TACAATTATT CCTTACTATG GACAAAAACA TCAATCTGAT ATCACTGATA TTGTAAGTAG TTTGCAATTA CAGTTCGAAT CATCGGAAGA AGCAGATAAG GGAAATAGCC ACAGTAAAAA AATGCTTAAA GCACTTCTAA GTGAGGGTGA AAGCATCTGG GAGATCACTG AGAAAATACT AAATTCGTTT GAGTATACTT CGAGATTTAC AAAAACAAAA ACTTTATACC AATTCCTCTT CCTAGCTACT TTCATCAATT GTGGAAGATT CAGCGATATT AAGAACGTTG ATCCGAAATC ATTTAAATTA GTCCAAAATA AGTATCTGGG AGTAATAATC CAGTGTTTAG TGACAGAGAC AAAGACAAGC GTTAGTAGGC ACATATACTT CTTTAGCGCA AGGGGTAGGA TCGATCCACT TGTATATTTG GATGAATTTT TGAGGAATTC TGAACCAGTC CTAAAACGAG TAAATAGGAC CGGCAATTCT TCAAGCAATA AACAGGAATA CCAATTATTA AAAGATAACT TAGTCAGATC GTACAATAAA GCTTTGAAGA AAAATGCGCC TTATTCAATC TTTGCTATAA AAAATGGCCC AAAATCTCAC ATTGGAAGAC ATTTGATGAC CTCATTTCTT TCAATGAAGG GCCTAACGGA GTTGACTAAT GTTGTGGGAA ATTGGAGCGA TAAGCGTGCT TCTGCCGTGG CCAGGACAAC GTATACTCAT CAGATAACAG CAATACCTGA TCACTACTTC GCACTAGTTT CTCGGTACTA TGCATATGAT CCAATATCAA AGGAAATGAT AGCATTGAAG GATGAGACTA ATCCAATTGA GGAGTGGCAG CATATAGAAC AGCTAAAGGG TAGTGCTGAA GGAAGCATAC GATACCCCGC ATGGAATGGG ATAATATCAC AGGAGGTACT AGACTACCTT TCATCCTACA TAAATAGACG CATATAAGTA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC CTATTCTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGGCT TTGTTGAATA AATCGAACTT TTGCTGAGTT GAAGGATCAG ATCACGCATC TTCCCGACAA CGCAGACCGT TCCGTGGCAA AGCAAAAGTT CAAAATCACC AACTGGTCCA CCTACAACAA AGCTCTCATC AACCGTGGCT CCCTCACTTT CTGGCTGGAT GATGGGGCGA TTCAGGCCTG GTATGAGTCA GCAACACCTT CTTCACGAGG CAGACCTCAG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CCTAGAGCGC ACGAATGAGG GCCGACAGGA AGCAAAGCTG AAAGGAATCA AATTTGGCCG CAGGCGTACC GTGGACAGGA ACGTCGTGCT GACGCTTCAT CAGAAGGGCA CTGGTGCAAC GGAAATTGCT CATCAGCTCA GTATTGCCCG CTCCACGGTT TATAAAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTTTAGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG TAATGATACC GATGAAACGC GAGAGGATGC TCACAATACG GGTTACTGAT GATGAACATG CCCGGTTACT TGAACGCTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGTCTGCC TGGGGGAGCC GGTTGCCCGT TCCGGAAAAC TGCCGACACT GGCACCGCCG TTACTGCGTC AGCTGGCCGC CATCGGAAAT AACCTGAATC AGACAGCCCG TAAGGTGAAC AGCGGGCAGT GGTCTTCCGG TGACCGGGTT CAGGTGGTGG CCGCACTGAT GGCCATCGGG GATGAGCTGC GCCGGCTGCG TCTGGCTGTC AGGGAACAGG GGGCGCGGGA TGATAGTTAA ATTTCATGCC AGGGGAAAAG GTGGTGGCAG TGGTCCGGTT GATTACCTGC TGGGGAGGGA GCGTAACCGC GAAGGCGCAA CGGTGCTTCA GGGTAATCCG GAAGAAGTCC GGGAACTCAT CGATGCCACG CCATTTGCGA AGAAATACAC GTCCGGTGTT CTGTCGTTCG CGGAGAAGGA GCTGCCGCCG GGAGGACGTG AAAAAGTGAT GGCGAGCTTT GAGCGTGTAC TGATGCCCGG TCTCGAAAAA AATCAGTACA GCATCCTGTG GGTGGAGCAC CAGGACAAGG GACGGCTTGA GCTGAATTTT GTCATTCCGA ACATGGAGCT ACAGACCGGA AAACGCCTCC AGCCGTACTA CGACCGCGCA GACAGGCCTA GAATTGATGC CTGGCAGACG CTGGTAAATC ACCATTACGG GCTGCATGAC CCGAACGCCC CGGAGAACCG CAGGACGCTG ACACTCCCTG ATAACCTGCC TGAAACGAAA CAGGCGCTAT TTCTTCCAGA ATTGCCATGA TTTTTTCCCC ACGGGAGGCG TCACTGGCTC CCGTGTTGTC GGCAGCTTTG ATTCGATAAG CAGCATCGCC TGTTTCAGGC TGTCTATGTG TGACTGTTGA GCTGTAACAA GTTGTCTCAG GTGTTCAATT TCATGTTCTA GTTGCTTTGT TTTACTGGTT TCACCTGTTC TATTAGGTGT TACATGCTGT TCATCTGTTA CATTGTCGAT CTGTTCATGG TGAACAGCTT TAAATGCACC AAAAACTCGT AAAAGCTCTG ATGTATCTAT CTTTTTTACA CCGTTTTCAT CTGTGCATAT GGACAGTTTT CCCTTTGATA TGTAACGGTG AACAGTTGTT CTACTTTTGT TTGTTAGTCT TGATGCTTCA CTGATAGATA CAAGAGCCAT AAGAACCTCA GATCCTTCCG TATTTAGCCA GTATGTTCTC TAGTGTGGTT CGTTGTTTTT GCGTGAGCCA TGAGAACGAA CCATTGAGAT CATACTTACT TTGCATGTCA CTCAAAAATT TTGCCTCAAA ACTGGTGAGC TGAATTTTTG CAGTTAAAGC ATCGTGTAGT GTTTTTCTTA GTCCGTTATG TAGGTAGGAA TCTGATGTAA TGGTTGTTGG TATTTTGTCA CCATTCATTT TTATCTGGTT GTTCTCAAGT TCGGTTACGA GATCCATTTG TCTATCTAGT TCAACTTGGA AAATCAACGT ATCAGTCGGG CGGCCTCGCT TATCAACCAC CAATTTCATA TTGCTGTAAG TGTTTAAATC TTTACTTATT GGTTTCAAAA CCCATTGGTT AAGCCTTTTA AACTCATGGT AGTTATTTTC AAGCATTAAC ATGAACTTAA ATTCATCAAG GCTAATCTCT ATATTTGCCT TGTGAGTTTT CTTTTGTGTT AGTTCTTTTA ATAACCACTC ATAAATCCTC ATAGAGTATT TGTTTTCAAA AGACTTAACA TGTTCCAGAT TATATTTTAT GAATTTTTTT AACTGGAAAA GATAAGGCAA TATCTCTTCA CTAAAAACTA ATTCTAATTT TTCGCTTGAG AACTTGGCAT AGTTTGTCCA CTGGAAAATC TCAAAGCCTT TAACCAAAGG ATTCCTGATT TCCACAGTTC TCGTCATCAG CTCTCTGGTT GCTTTAGCTA ATACACCATA AGCATTTTCC CTACTGATGT TCATCATCTG AACGTATTGG TTATAAGTGA ACGATACCGT CCGTTCTTTC CTTGTAGGGT TTTCAATCGT GGGGTTGAGT AGTGCCACAC AGCATAAAAT TAGCTTGGTT TCATGCTCCG TTAAGTCATA GCGACTAATC GCTAGTTCAT TTGCTTTGAA AACAACTAAT TCAGACATAC ATCTCAATTG GTCTAGGTGA TTTTAATCAC TATACCAATT GAGATGGGCT AGTCAATGAT AATTACTAGT CCTTTTCCTT TGAGTTGTGG GTATCTGTAA ATTCTGCTAG ACCTTTGCTG GAAAACTTGT AAATTCTGCT AGACCCTCTG TAAATTCCGC TAGACCTTTG TGTGTTTTTT TTGTTTATAT TCAAGTGGTT ATAATTTATA GAATAAAGAA AGAATAAAAA AAGATAAAAA GAATAGATCC CAGCCCTGTG TATAACTCAC TACTTTAGTC AGTTCCGCAG TATTACAAAA GGATGTCGCA AACGCTGTTT GCTCCTCTAC AAAACAGACC TTAAAACCCT AAAGGCTTAA GTAGCACCCT CGCAAGCTCG GGCAAATCGC TGAATATTCC TTTTGTCTCC GACCATCAGG CACCTGAGTC GCTGTCTTTT TCGTGACATT CAGTTCGCTG CGCTCACGGC TCTGGCAGTG AATGGGGGTA AATGGCACTA CAGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTGATGTCCG GCGGTGCTTT TGCCGTTACG CACCACCCCG TCAGTAGCTG AACAGGAGGG ACAGCTGATA GAAACAGAAG CCACTGGAGC ACCTCAAAAA CACCATCATA CACTAAATCA GTAAGTTGGC AGCATCACCC GACGCACTTT GCGCCGAATA AATACCTGTG ACGGAAGATC ACTTCGCAGA ATAAATAAAT CCTGGTGTCC CTGTTGATAC CGGGAAGCCC TGGGCCAACT TTTGGCGAAA ATGAGACGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CTAACTTCGA TCACTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG ACCTGATTTT TTAACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA TTTTTTTAAG GCAGTTATTG GTGCCCTTAA ACGCCTGGTT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAA GCAAATTCGA CCCGGTCGTC GGTTCAGGGC AGGGTCGTTA AATAGCCGCT TATGTCTATT GCTGGTTTAC CGGTTTATTG ACTACCGGAA GCAGTGTGAC CGTGTGCTTC TCAAATGCCT GAGGCCAGTT TGCTCAGGCT CTCCCCGTGG AGGTAATAAT TGACGATATG ATCATTTATT CTGCCTCCCA GAGCCTGATA AAAACGGTTA GCGCCAGCAA CCGCACCTGT GGCGCTGAGG TCTGCCTCGT GAAGAAGGTG TTGCTGACTC ATACCAGGCC TGAATCGCCC CATCATCCAG CCAGAAAGTG AGGGAGCCAC GGTTGATGAG AGCTTTGTTG TAGGTGGACC AGTTGGTGAT TTTGAACTTT TGCTTTGCCA CGGAACGGTC TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA GCAAAAGTTC GATTTATTCA ACAAAGCCAC GTTGTGTCTC
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