pECMV-CSE1L(human)-V5-2×Strep-SV40-Puro
$84.00
In stock
SKU
PL44957
NM_001316.4;CAS; CSE1; XPO2
100μg liquid plasmid(Instantly):$105
| name | pECMV-CSE1L(human)-V5-2×Strep-SV40-Puro | another name | apECMV-CSE1L(human)-V5-2×Strep-SV40-Puro |
| Insert Size (bp) | 2916bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG GAACTCAGCG ATGCAAATCT GCAAACACTA ACAGAATATT TAAAGAAAAC ACTTGATCCT GATCCTGCCA TCCGACGTCC AGCTGAGAAA TTTCTTGAAT CTGTTGAAGG AAATCAGAAT TATCCACTGT TGCTTTTGAC ATTACTGGAG AAGTCCCAGG ATAATGTTAT CAAAGTATGT GCTTCAGTAA CATTCAAAAA CTATATTAAA AGGAACTGGA GAATTGTTGA AGATGAACCA AACAAAATTT GTGAAGCCGA TCGAGTGGCC ATTAAAGCCA ACATAGTGCA CTTGATGCTT AGCAGCCCAG AGCAAATTCA GAAGCAGTTA AGTGATGCAA TTAGCATTAT TGGCAGAGAA GATTTTCCAC AGAAATGGCC TGACTTGCTG ACAGAAATGG TGAATCGCTT TCAGAGTGGA GATTTCCATG TTATTAATGG AGTCCTCCGT ACAGCACATT CATTATTTAA AAGATACCGT CATGAATTTA AGTCAAACGA GTTATGGACT GAAATTAAGC TTGTTCTGGA TGCCTTTGCT TTGCCTTTGA CTAATCTTTT TAAGGCCACT ATTGAACTCT GCAGTACCCA TGCAAATGAT GCCTCTGCCC TGAGGATTCT GTTTTCTTCC CTGATCCTGA TCTCAAAATT GTTCTATAGT TTAAACTTTC AGGATCTCCC TGAATTTTTT GAAGATAATA TGGAAACTTG GATGAATAAT TTTCATACTC TCTTAACATT GGATAATAAG CTTTTACAAA CTGATGATGA AGAGGAAGCC GGCTTATTGG AGCTCTTAAA ATCCCAGATT TGTGATAATG CCGCACTCTA TGCACAAAAG TACGATGAAG AATTCCAGCG ATACCTGCCT CGTTTTGTTA CAGCCATCTG GAATTTACTA GTTACAACGG GTCAAGAGGT TAAATATGAT TTGTTGGTAA GTAATGCAAT TCAATTTCTG GCTTCAGTTT GTGAGAGACC TCATTATAAG AATCTATTTG AGGACCAGAA CACGCTGACA AGTATCTGTG AAAAGGTTAT TGTGCCTAAC ATGGAATTTA GAGCTGCTGA TGAAGAAGCA TTTGAAGATA ATTCTGAGGA GTACATAAGG AGAGATTTGG AAGGATCTGA TATTGATACT AGACGCAGGG CTGCTTGTGA TCTGGTACGA GGATTATGCA AGTTTTTTGA GGGACCTGTG ACAGGAATCT TCTCTGGTTA TGTTAATTCC ATGCTGCAGG AATACGCAAA AAATCCATCT GTCAACTGGA AACACAAAGA TGCAGCCATC TACCTAGTGA CATCTTTGGC ATCAAAAGCC CAAACACAGA AGCATGGAAT TACACAAGCA AATGAACTTG TAAACCTAAC TGAGTTCTTT GTGAATCACA TCCTCCCTGA TTTAAAATCA GCTAATGTGA ATGAATTTCC TGTCCTTAAA GCTGACGGTA TCAAATATAT TATGATTTTT AGAAATCAAG TGCCAAAAGA ACATCTTTTA GTCTCGATTC CTCTCTTGAT TAATCATCTT CAAGCTGAAA GTATTGTTGT TCATACTTAC GCAGCTCATG CTCTTGAACG GCTCTTTACT ATGCGAGGGC CTAACAATGC CACTCTCTTT ACAGCTGCAG AAATCGCACC GTTTGTTGAG ATTCTGCTAA CAAACCTTTT CAAAGCTCTC ACACTTCCTG GCTCTTCAGA AAATGAATAT ATTATGAAAG CTATCATGAG AAGTTTTTCT CTCCTACAAG AAGCCATAAT CCCCTACATC CCTACTCTCA TCACTCAGCT TACACAGAAG CTATTAGCTG TTAGTAAGAA CCCAAGCAAA CCTCACTTTA ATCACTACAT GTTTGAAGCA ATATGTTTAT CCATAAGAAT AACTTGCAAA GCTAACCCTG CTGCTGTTGT AAATTTTGAG GAGGCTTTGT TTTTGGTGTT TACTGAAATC TTACAAAATG ATGTGCAAGA ATTTATTCCA TACGTCTTTC AAGTGATGTC TTTGCTTCTG GAAACACACA AAAATGACAT CCCGTCTTCC TATATGGCCT TATTTCCTCA TCTCCTTCAG CCAGTGCTTT GGGAAAGAAC AGGAAATATT CCTGCTCTAG TGAGGCTTCT TCAAGCATTC TTAGAACGCG GTTCAAACAC AATAGCAAGT GCTGCAGCTG ACAAAATTCC TGGGTTACTA GGTGTCTTTC AGAAGCTGAT TGCATCCAAA GCAAATGACC ACCAAGGTTT TTATCTTCTA AACAGTATAA TAGAGCACAT GCCTCCTGAA TCAGTTGACC AATATAGGAA ACAAATCTTC ATTCTGCTAT TCCAGAGACT TCAGAATTCC AAAACAACCA AGTTTATCAA GAGTTTTTTA GTCTTTATTA ATTTGTATTG CATAAAATAT GGGGCACTAG CACTACAAGA AATATTTGAT GGTATACAAC CAAAAATGTT TGGAATGGTT TTGGAAAAAA TTATTATTCC TGAAATTCAG AAGGTATCTG GAAATGTAGA GAAAAAGATC TGTGCGGTTG GCATAACCAA ATTACTAACA GAATGTCCCC CAATGATGGA CACTGAGTAT ACCAAACTGT GGACTCCATT ATTACAGTCT TTGATTGGTC TTTTTGAGTT ACCCGAAGAT GATACCATTC CTGATGAGGA ACATTTTATT GACATAGAAG ATACACCAGG ATATCAGACT GCCTTCTCAC AGTTGGCATT TGCTGGGAAA AAAGAGCATG ATCCTGTAGG TCAAATGGTG AATAACCCCA AAATTCACCT GGCACAGTCA CTTCACAAGT TGTCTACCGC CTGTCCAGGA AGGGTTCCAT CAATGGTGAG CACCAGCCTG AATGCAGAAG CGCTCCAGTA TCTCCAAGGG TACCTTCAGG CAGCCAGTGT GACACTGCTT GCGGCCGCGG GTAGCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGGGT AGCTGGAGCC ATCCGCAGTT TGAAAAAGGT GGTGGTAGCG GTGGTGGTTC AGGTGGTAGC AGTGCATGGT CACACCCTCA GTTTGAGAAA TGACTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGT
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