pPLCG2(human)-SNP-miniP-Fluc
$70.00
In stock
SKU
PL44952
100μg liquid plasmid(Instantly):$91
| name | pPLCG2(human)-SNP-miniP-Fluc | another name | apPLCG2(human)-SNP-miniP-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Element |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCG GAAAACCACT GAAGACGTGC CTGAGCTAAG CCACACTGCC AAACTGCCCT CCATAGAGAT CGTGGGCGGG GATGCAAATA GGTGGAGGTT GGAGTACGCT CTTTTGGTAT GACTTCGCTG AGTTTTGTTG AACTTTTTGC CGTTTGCACA ACTGAGAGGT GAAAACTGAT ACATCGTCGT CCATTAGAGC TTCTTCTTGT AAGGGACATT AACCAGTTTT TCCCTCATTT AGGGAGCAGT GGCATTTTCT TTTCTGGGAA CTATTTGTGC TTCTTACCTG ATGTATCTAC TGCAGTAGGT ATTTATAGCA TAGATGTATC AGAACTAAGA GGTTAAAAAT TTATATTCAC ATATTTGTCA TTTGAATTAA AATTGCCTAT GCAATTTATT GCTACATAGA CACTTTTAGC AAGTTTGAAT CTTTTCTTTT ATGATTCGGG ATTTGGTGAC ATACTTGGAA AGGTGTTCCC CAGTCTGGGA GTTTGCTGTC TAGAAACCCT ATATTTTCTT TGAGTGAGAT ACCTATTTGA ACAGCTGCCT CACATTAATT TGGGGAAAAG GTGAAATTCA TTCCCCACCT AGAAACTCCT GGCCTAGGGG GCCAGCGTGC TCATGCCTGA CTTACAGCAG GCGTTCACTT TCCTTCCCAG ATAAGTACAT GCAGATGAAT CACGCATTGT TTTCTCTCAA TGGGCGCACG GGCTACGTTC TGCAGCCTGA GAGCATGAGG ACAGAGAAAT ATGACCCGAT GCCACCCGAG TCCCAGAGGA AGATCCTGAT GACGCTGACA GTCAAGGTAA AGCCAGCCCT CCCTTCCTGC CAGGGGAGCC AGCCGCCCTC CCTGGGGGCT GGGCCGATGC TGTCTTGAGA GCAGGGAACC CATGTCTAGG GAGGCTGGTT GTCTTGTTTA AACGATCCCC ATTCAGGCAG TGTTTGAGGA TCTTTCTTTC TTACAGTACA CTCCGGGGTC GTTCTGGGAG AAGAGGGAAG AGAGGGGAAA CCTCCTCAAA CCCACTTCCC CTTGTCCTCT GTGGGGGTGA GGTATGACCC TATCTTTCTG GGGTCCTGGT GAGGTCAGCC TCTGGCCGGA AAGAGAAAGA AAACATGGGA AACAGCCATT TGGTCTTAAT GGAGAAGTTT TTTCCCTTAC TTTGTCCAAT TCCAGGGCTT TTTAAAATTC ATCTGACTTT CAGAGAAAGT GTTGTTGCTT TGTAAGAATT TTTGAAAAGT AGAGAAATGA ATTGCATTTC TCCTAAGGTT AAGGGCGGGC GGGTGGTAGG GGGAAGAAGC ATACGTAAAC ACAGGCCTTT AAATAAACAG CAGTTATAAA AAGAATGCCA CACTGTTCCT CAGCCTTTCC TAGGGGTGTG GAAATCCAGT GATTACTCGA GGATATCAAG ATCTGGCCTC GGCGGCCAAG CTTAGACACT AGAGGGTATA TAATGGAAGC TCGACTTCCA GCTTGGCAAT CCGGTACTGT TGGTAAAGCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC CATTCTACCC ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT ACGCCCTGGT GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT ACGCCGAGTA CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG AGCTGCTGAA CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC TGCAAAAGAT CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC GCACCGCTTG TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC CCGACACCGC TATCCTCAGC GTGGTGCCAT TTCACCACGG CTTCGGCATG TTCACCACGC TGGGCTACTT GATCTGCGGC TTTCGGGTCG TGCTCATGTA CCGCTTCGAG GAGGAGCTAT TCTTGCGCAG CTTGCAAGAC TATAAGATTC AATCTGCCCT GCTGGTGCCC ACACTATTTA GCTTCTTCGC TAAGAGCACT CTCATCGACA AGTACGACCT AAGCAACTTG CACGAGATCG CCAGCGGCGG GGCGCCGCTC AGCAAGGAGG TAGGTGAGGC CGTGGCCAAA CGCTTCCACC TACCAGGCAT CCGCCAGGGC TACGGCCTGA CAGAAACAAC CAGCGCCATT CTGATCACCC CCGAAGGGGA CGACAAGCCT GGCGCAGTAG GCAAGGTGGT GCCCTTCTTC GAGGCTAAGG TGGTGGACTT GGACACCGGT AAGACACTGG GTGTGAACCA GCGCGGCGAG CTGTGCGTCC GTGGCCCCAT GATCATGAGC GGCTACGTTA ACAACCCCGA GGCTACAAAC GCTCTCATCG ACAAGGACGG CTGGCTGCAC AGCGGCGACA TCGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA CCGGCTGAAG AGCCTGATCA AATACAAGGG CTACCAGGTA GCCCCAGCCG AACTGGAGAG CATCCTGCTG CAACACCCCA ACATCTTCGA CGCCGGGGTC GCCGGCCTGC CCGACGACGA TGCCGGCGAG CTGCCCGCCG CAGTCGTCGT GCTGGAACAC GGTAAAACCA TGACCGAGAA GGAGATCGTG GACTATGTGG CCAGCCAGGT TACAACCGCC AAGAAGCTGC GCGGTGGTGT TGTGTTCGTG GACGAGGTGC CTAAAGGACT GACCGGCAAG TTGGACGCCC GCAAGATCCG CGAGATTCTC ATTAAGGCCA AGAAGGGCGG CAAGATCGCC GTGTAATAAT TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGCG GCCGCAAATG CTAAACCACT GCAGTGGTTA CCAGTGCTTG ATCAGTGAGG CACCGATCTC AGCGATCTGC CTATTTCGTT CGTCCATAGT GGCCTGACTC CCCGTCGTGT AGATCACTAC GATTCGTGAG GGCTTACCAT CAGGCCCCAG CGCAGCAATG ATGCCGCGAG AGCCGCGTTC ACCGGCCCCC GATTTGTCAG CAATGAACCA GCCAGCAGGG AGGGCCGAGC GAAGAAGTGG TCCTGCTACT TTGTCCGCCT CCATCCAGTC TATGAGCTGC TGTCGTGATG CTAGAGTAAG AAGTTCGCCA GTGAGTAGTT TCCGAAGAGT TGTGGCCATT GCTACTGGCA TCGTGGTATC ACGCTCGTCG TTCGGTATGG CTTCGTTCAA CTCTGGTTCC CAGCGGTCAA GCCGGGTCAC ATGATCACCC ATATTATGAA GAAATGCAGT CAGCTCCTTA GGGCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCGGTGT TGTCGCTCAT GGTAATGGCA GCACTACACA ATTCTCTTAC CGTCATGCCA TCCGTAAGAT GCTTTTCCGT GACCGGCGAG TACTCAACCA AGTCGTTTTG TGAGTAGTGT ATACGGCGAC CAAGCTGCTC TTGCCCGGCG TCTATACGGG ACAACACCGC GCCACATAGC AGTACTTTGA AAGTGCTCAT CATCGGGAAT CGTTCTTCGG GGCGGAAAGA CTCAAGGATC TTGCCGCTAT TGAGATCCAG TTCGATATAG CCCACTCTTG CACCCAGTTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCGGGGTGT GCAAAAACAG GCAAGCAAAA TGCCGCAAAG AAGGGAATGA GTGCGACACG AAAATGTTGG ATGCTCATAC TCGTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA CTAGTACGTC TCTCAAGGAT AAGTAAGTAA TATTAAGGTA CGGGAGGTAT TGGACAGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG TACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
